MORPHOLOGY AND PATHOLOGICAL CHANGES OF POLYTETRAFLUOROETHYLENE ARTIFICIAL TRACHEAL TRANSPLANTATION_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

LIGang ¹ , ZHANGJing ¹ , CUIXiaohai ¹ , ZHAOYang ⁴ , CAOPeilong ² , LULu ³ ,  LIXiaojun ¹

1. The 2nd Department of Thoracic Surgery the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an Shaanxi, 710061, P. R. China;
2. Department of Pathology, Xi'an Shaanxi the First Affiliated Hospital of Xi'an Jiaotong University, 710061, P. R. China;
3. Department of Operating Room, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an Shaanxi, 710061, P. R. China;
4. Department of Tumor Surgery, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an Shaanxi, 710061, P. R. China;

Corresponding author：

LIXiaojun, Email: dooopenit@163.com

Keywords：

Artificial tracheal prosthesis; Tracheal reconstruction; Polytetrafluoroethylene; EpithelizationRabbit

DOI：

10.7507/1002-1892.20140194

Video：

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Objective To observe the morphological and pathological changes after transplantation of polytetrafluoroethylene (PTFE) in vivo. Methods PTFE microporous polypropylene tube which was encircled by spiral steel wire was used to prepare the artificial trachea.Forty New Zealand white rabbits (weighing,4-5 kg) were selected,and were divided into 2 groups.After the cervical trachea (2 cm in length) was removed,the end-to-end anastomosis between the trachea and PTFE artificial trachea was performed in the experimental group (n=20),and end-to-end anastomosis of the trachea in the control group (n=20).The survival of the rabbits was observed after operation;the X-ray,gross,and histological observations were carried out at 2,4,and 6 months after operation.The longitudinal tensile and radial support biomechanical tests were performed before and after transplantation. Results The survival time was more than 2 months and the artificial airway was patency in 15 rabbits of the experimental group;the tissue outside the artificial trachea was like tracheal tissue,which filled in the defect,but it was more than 4 months.X-ray observation showed that the PTFE artificial trachea had no obvious displacement in the experimental group,and no tracheostenosis was observed in the control group.After 2 months,there was no epithelial tissue on the artificial airway wall;after 4 months,there was some epithelial cells on the artificial airway wall,incomplete endothelialization and trachea layer structure were seen with no tracheal ciliated columnar epithelium;after 6 months,the artificial trachea wall was covered with epithelium basically,and some ciliated columnar epithelium cells were found,which had the physiological function of the trachea.The transplanted PTFE artificial trachea could keep the stability of the biological mechanics performance,and could be used for the rabbit tracheal reconstruction. Conclusion PTFE artificial trachea can induce to form a tracheal tissue in the trachea tissues of recipients,each layer of the trachea is relatively complete and the experiment animals can be short-term survival.

Citation： LIGang, ZHANGJing, CUIXiaohai, ZHAOYang, CAOPeilong, LULu, LIXiaojun. MORPHOLOGY AND PATHOLOGICAL CHANGES OF POLYTETRAFLUOROETHYLENE ARTIFICIAL TRACHEAL TRANSPLANTATION. Chinese Journal of Reparative and Reconstructive Surgery, 2014, 28(7): 879-884. doi: 10.7507/1002-1892.20140194 Copy

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17.	陈万生,焦英华,郝雁冰,等.人工气管移植的病理学变化.中华胸心血管外科杂志,2013,29(2):99-101.
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1. Delaere PR.Tracheal transplantation.Curr Opin Pulm Med,2012,18(4):313-320.
2. Fishman JM,De Coppi P,Elliott MJ,et al.Airway tissue engineering.Expert Opin Biol Ther,2011,11(12):1623-1635.
3. 王嵩,史宏灿.人工气管研究进展.中国修复重建外科杂志,2011,25(3):365-368.
4. 姚佳,李晓军,崔晓海,等.兔人工气管移植后成活时间与C-反应蛋白的相关性.中国组织工程研究,2014,18(10):718-723.
5. Osada H.Artificial trachea.J Bronchol,2006,13(1):39-43.
6. 刘鑫,姜召阳,秦永.碳-碳复合材料气管重建的实验研究.中华耳鼻咽喉头颈外科杂志,2010,45(8):669-673.
7. Jungebluth P,Bader A,Baiguera S,et al.The concept of in vivo airway tissue engineering.Biomaterials,2012,33(17):4319-4326.
8. Du XF,Kwon SK,Song JJ,et al.Tracheal reconstruction by mesenchymal stem cells with small intestine submucosa in rabbits.Int J Pediatr Otorhinolaryngol,2012,76(3):345-351.
9. Haag J,Baiguera S,Jungebluth P,et al.Biomechanical and angiogenic properties of tissue-engineered rat trachea using genipin cross-linked decellularized tissue.Biomaterials,2012,33(3):780-789.
10. Baiguera S,Del Gaudio C,Jaus MO,et al.Long-term changes to in vitro preserved bioengineered human trachea and their implications for decellularized tissues.Biomaterials,2012,33(14):3662-3672.
11. Zhao F,Zhang Y,Liu S.Artificial trachea reconstruction with two-stage approach using memory-alloy mesh.Chin Med J (Engl),2003,116(12):1949-1951.
12. 袁海根,曾金芳,杨杰.聚四氟乙烯改性研究进展.塑料工业,2005,3(8):7-10.
13. 史宏灿,徐志飞,赵学维,等.人工气管重建气管的实验研究.中华胸心血管外科杂志,2004,20(2):70-72.
14. 林海平,曹子昂,顾旭东,等.胶原涂层记忆合金人工气管的构建.上海交通大学学报:医学版,2007,27(11):1331-1334.
15. Neville WE,Bolanowski JP,Kotia GG.Clinical experience with the silicone tracheal prothesis.J Thorac Cardiovasc Surg,1990,99(4):604-613.
16. Kanemaru S,Hirano S,Umeda H,et al.A tissue-engineering approach for stenosis of the trachea and/or cricoid.Acta Otolaryngol Suppl,2010,(563):79-83.
17. 陈万生,焦英华,郝雁冰,等.人工气管移植的病理学变化.中华胸心血管外科杂志,2013,29(2):99-101.
18. 赵凤瑞,张银合,刘德若,等.记忆合金网二期成形人工气管置换术.中华外科杂志,2003,4(3):201-204.
19. 寇秀娟,陈晓红.长段气管缺损后重建术的研究进展.国际耳鼻喉头颈外科杂志,2012,36(5):266-269.
20. 陈坚伟,张迪.3D打印技术医学应用综述与展望.电脑知识与技术,2013,19(15):3632-3633.

Chinese Journal of Reparative and Reconstructive Surgery

MORPHOLOGY AND PATHOLOGICAL CHANGES OF POLYTETRAFLUOROETHYLENE ARTIFICIAL TRACHEAL TRANSPLANTATION

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