SUNKai 1,2,3 , NIANZhenghao 1,2,3 , XUCheng 2 , LIRuixin 2 , LIHui 3
  • 1. Graduate School of Tianjin Medical University, Tianjin, 300070, P. R. China;
  • 2. Institute of Medical Equipment, Academy of Military and Medical Sciences;
  • 3. Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, 300070, P. R. China;
LIRuixin, Email: limxinxin@163.com; LIHui, Email: ortholivea@126.com
Cartilage tissue engineering; Silk fibroin; Collagen; Composite scaffold; Bone marrow mesenchymal stem cells; Rat
10.7507/1002-1892.20140198
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Objective To prepare composite scaffold of different quality ratio of silk fibroin to collagen,analyze the scaffold performance,and optimize the quality ratio for chondrocyte tissue engineering. Methods The silk fibroin/collagen composite scaffold was made using a freeze-drying technique in different quality ratios of silk fibroin to collagen:4:2(group A),4:4(group B),and 4:8(group C).The porosity,water absorption expansion rate,mechanical properties,and pore size of composite scaffold were detected.The bone marrow mesenchymal stem cells (BMSCs) were isolated from 4-week-old male Wistar rats by density gradient centrifugation,and the third generation BMSCs were seeded onto the scaffolds at 2×107 cells/mL density,and were cultured for 14 days.The cell proliferation was detected using MTT assay at 1,3,5,7,9,11,and 13 days,the cell morphology and distribution were observed by HE staining and scanning electron microscopy (SEM). Results The porosity of groups A,B,and C was 94.6%±1.6%,80.6%±1.1%,and 60.6%±1.0% respectively;and significant differences were found between group A and groups B and C,and between group B and group C (P<0.05).The water absorption expansion rates of groups A,B,and C were 1 523.7%±186.6%,1 091.0%±151.6%,and 659.6%±161.4% respectively,showing no significant difference among 3 groups (F=6.67,P=0.08).The elasticity modulus of groups A,B,and C were (23.1±2.5),(25.1±2.3),and (29.8±2.6) kPa respectively,showing no significant difference among 3 groups (F=2.00,P=0.28).The pore size of groups A,B,and C was (103±12),(80±15),and (60±16)μm respectively,showing no significant difference among 3 groups (F=2.22,P=0.26).MTT results showed that the cell proliferation in the group A at 7,9,11,and 13 days were better than those in groups B and C (P<0.05);at 14 days after cultivation,even pore size,good intercommunicating of holes,and good cells growth on the scaffolds with full extension and more extracellular matrix were seen under SEM in group A,but small pore size,poor intercommunicating of holes and poor cell growth on the scaffolds in groups B and C.HE staining and SEM results showed that the cells on the scaffold in group A was obviously more than those in groups B and C. Conclusion The scaffold prepared in a quality ratio 4:2 of silk fibroin to collagen has better porosity,water absorption expansion rate,elasticity modulus,and pore size,on which the cells can grow well,so it is more suitable for cartilage tissue engineering.

Citation： SUNKai, NIANZhenghao, XUCheng, LIRuixin, LIHui. PREPARATION AND PERFORMANCE RESEARCH OF SILK FIBROIN COLLAGEN BLEND SCAFFOLD. Chinese Journal of Reparative and Reconstructive Surgery, 2014, 28(7): 903-908. doi: 10.7507/1002-1892.20140198 Copy

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