EXPERIMENTAL STUDY ON EFFECT OF BURN ON BROWN ADIPOSE TISSUE IN MICE_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

ZHUANGShubo ^1,2 ,  CHAIJiake ¹ , YUYonghui ¹ , YINHuinan ¹ , LIULingying ¹ , FANJun ¹ , WANGYihe ² , HOUYusen ²

1. Department of Burn and Plastic Surgery, the First Hospital Affiliated to Chinese PLA General Hospital, Beijing, 100048, P. R. China;
2. Medical School of Chinese PLA, Beijing, 100048, P. R. China;

Corresponding author：

CHAIJiake, Email: cjk304@126.com

Keywords：

Burn; Hypermetabolism; Brown adipose tissue; Uncoupling protein 1; Mice

DOI：

10.7507/1002-1892.20140218

Video：

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Objective To investigate the effect of burn on brown adipose tissue (BAT) in BALB/c mice. Methods Forty 3-4 months old male BALB/c mice with initial body weight of (20±3) g were randomly divided into control group and burn group (n=20).BALB/c mice in burn group were subjected to a 30% total body surface area (TBSA) full-thickness thermal injury.BALB/c mice in control group were not treated.The body weight and temperature were observed before and after burn.At 7 days after burn,morphological changes of white adipose tissue (WAT) and BAT were observed,the gene and protein expressions of uncoupling protein 1 (UCP-1) were detected. Results There was no significant difference in the body weight and body temperature before burn (P>0.05).At 1,2,3,and 4 weeks after burn,the body weight was significantly lower in burn group than in control group (P<0.05).At 1,2,3,and 7 days after burn,the body temperature was significantly higher in burn group than in control group (P<0.05).At 7 days after burn,the weight of WAT was significantly reduced,and the weight of BAT was significantly increased in burn group (P<0.05); WAT and BAT cells became smaller,cell number increased,the cytoplasm and mitochondria appeared as compact.The UCP-1 gene and protein expressions of burn group were significantly higher than those of control group (P<0.05). Conclusion BAT plays an important role in burn-induced hypermetabolism.

Citation： ZHUANGShubo, CHAIJiake, YUYonghui, YINHuinan, LIULingying, FANJun, WANGYihe, HOUYusen. EXPERIMENTAL STUDY ON EFFECT OF BURN ON BROWN ADIPOSE TISSUE IN MICE. Chinese Journal of Reparative and Reconstructive Surgery, 2014, 28(8): 988-992. doi: 10.7507/1002-1892.20140218 Copy

1.	柴家科,申传安,盛志勇,等.严重烧伤脓毒症患者骨骼肌蛋白分解代谢的临床研究.中华医学杂志,2005,85(41):2895-2898.
2.	Ko Y,Yu YM,Zhao G,et al.Brown adipose tissue and its modulation by a mitochondria-targeted peptide in rat burn injury-induced hypermetabolism.Am J Physiol Endocrinol Metab,2013,304(4):E331-E341.
3.	Marzolla V,Armani A,Zennaro MC,et al.The role of the mineralocorticoid receptor in adipocyte biology and fat metabolism.Mol Cell Endocrinol,2012,350(2):281-288.
4.	Tam CS,Lecoultre V,Ravussin E.Brown adipose tissue:mechanisms and potential therapeutic targets.Circulation,2012,125(22):2782-2791.
5.	Drubach LA,Palmer EL 3rd,Connolly LP,et al.Pediatric brown adipose tissue:detection,epidemiology,and differences from adults.J Pediatr,2011,159(6):939-944.
6.	Virtanen KA,Nuutila P.Brown adipose tissue in humans.Curr Opin Lipidol,2011,22(1):49-54.
7.	Zhang Q,Ma B,Fischman AJ,et al.Increased uncoupling protein 1 mRNA expression in mice brown adipose tissue after burn injury.J Burn Care Res,2008,29(2):358-362.
8.	Bartelt A,Merkel M,Heeren J.A new,powerful player in lipoprotein metabolism:brown adipose tissue.J Mol Med (Berl),2012,90(8):887-893.
9.	Kraft R,Kulp GA,Herndon DN,et al.Is there a difference in clinical outcomes,inflammation,and hypermetabolism between scald and flame burn? Pediatr Criti Care Med,2011,12(6):e275-281.
10.	Pawlik TM,Carter EA,Bode BP,et al.Central role of interleukin-6 in burn induced stimulation of hepatic amino acid transport.Int J Mol Med,2003,12(4):541-548.
11.	Zhang Q,Carter EA,Ma BY,et al.Molecular mechanism(s) of burn-induced insulin resistance in murine skeletal muscle:role of IRS phosphorylation.Life Sci,2005,77(24):3068-3077.
12.	郝岱峰,柴家科,吴焱秋.烫伤大鼠创面脓毒症发生后肝组织中脂代谢相关基因表达水平的变化.中华烧伤杂志,2006,22(4):250-253.
13.	Izamis ML,Uygun K,Uygun B,et al.Effects of burn injury on markers of hypermetabolism in rats.J Burn Care Res,2009,30(6):993-1001.
14.	解伟光,汪仕良,黎鳌.中国人烧伤后能量消耗中三大营养素的分布.第三军医大学学报,1992,14 (4):319-321.
15.	柴家科,盛志勇.应重视严重烧伤脓毒症患者骨骼肌蛋白高分解代谢的研究.中华医学杂志,2005,85(41):2883-2885.
16.	Bajzer M,Olivieri M,Haas MK,et al.Cannabinoid receptor 1 (CB1) antagonism enhances glucose utilisation and activates brown adipose tissue in diet-induced obese mice.Diabetologia,2011,54(12):3121-3131.
17.	Chaves VE,Frasson D,Garófalo MA,et al.Increased glyceride-glycerol synthesis in liver and brown adipose tissue of rat:in-vivo contribution of glycolysis and glyceroneogenesis.Lipids,2012,47(8):773-780.
18.	Merritt EK,Cross JM,Bamman MM.Inflammatory and protein metabolism signaling responses in human skeletal muscle after burn injury.J Burn Care Res,2012,33(2):291-297.
19.	Carter EA,Paul KW,Barrow SA,et al.Previous burn injury predisposes mice to lipopolysaccharide-induced changes in glucose metabolism.J Burn Care Res,2012,33(5):683-689.
20.	Yasuhara S,Kaneki M,Sugita H,et al.Adipocyte apoptosis after burn injury is associated with altered fat metabolism.J Burn Care Res,2006,27(3):367-376.
21.	Leone TC,Lehman JJ,Finck BN,et al.PGC-1alpha deficiency causes multi-system energy metabolic derangements:muscle dysfunction,abnormal weight control and hepatic steatosis.PLoS Biol,2005,3(4):e101.
22.	Wu J,Boström P,Sparks LM,et al.Beige adipocytes are a distinct type of thermogenic fat cell in mouse and human.Cell,2012,150(2):366-376.
23.	Bartelt A,Bruns OT,Reimer R,et al.Brown adipose tissue activity controls triglyceride clearance.Nat Med,2011,17(2):200-205.
24.	Chang Y,She ZG,Sakimura K,et al.Ablation of NG2 proteoglycan leads to deficits in brown fat function and to adult onset obesity.PLoS One,2012,7(1):e30637.
25.	Timmons JA,Wennmalm K,Larsson O,et al.Myogenic gene expression signature establishes that brown and white adipocytes originate from distinct cell lineages.Proc Natl Acad Sci U S A,2007,104(11):4401-4406.
26.	Cronin CG,Prakash P,Daniels GH,et al.Brown fat at PET/CT:correlation with patient characteristics.Radiology,2012,263(3):836-842.
27.	Kajimura S,Seale P,Kubota K,et al.Initiation of myoblast to brown fat switch by a PRDM16-C/EBP-beta transcriptional complex.Nature,2009,460(7259):1154-1158.
28.	Cypess AM,Kahn CR.Brown fat as a therapy for obesity and diabetes.Curr Opin Endocrinol Diabetes Obes,2010,17(2):143-149.
29.	Farmer SR.Molecular determinants of brown adipocyte formation and function.Genes Dev,2008,22(10):1269-1275.
30.	Okita N,Hayashida Y,Kojima Y,et al.Differential responses of white adipose tissue and brown adipose tissue to caloric restriction in rats.Mech Ageing Dev,2012,133(5):255-266.
31.	Sharon L,Jennifer H,Yuan X,et al.Cold-induced activation of brown adipose tissue and adipose angiogenesis in mice.Nature Protocols,2012,3(7):606-615.
32.	Lim S,Honek J,Xue Y,et al.Cold-induced activation of brown adipose tissue and adipose angiogenesis in mice.Nat Protoc,2012,7(3):606-615.
33.	Schulz TJ,Huang TL,Tran TT.Identification of inducible brown adipocyte progenitors residing in skeletal muscle and white fat.Proc Natl Acad Sci U S A,2011,108(1):143-148.

1. 柴家科,申传安,盛志勇,等.严重烧伤脓毒症患者骨骼肌蛋白分解代谢的临床研究.中华医学杂志,2005,85(41):2895-2898.
2. Ko Y,Yu YM,Zhao G,et al.Brown adipose tissue and its modulation by a mitochondria-targeted peptide in rat burn injury-induced hypermetabolism.Am J Physiol Endocrinol Metab,2013,304(4):E331-E341.
3. Marzolla V,Armani A,Zennaro MC,et al.The role of the mineralocorticoid receptor in adipocyte biology and fat metabolism.Mol Cell Endocrinol,2012,350(2):281-288.
4. Tam CS,Lecoultre V,Ravussin E.Brown adipose tissue:mechanisms and potential therapeutic targets.Circulation,2012,125(22):2782-2791.
5. Drubach LA,Palmer EL 3rd,Connolly LP,et al.Pediatric brown adipose tissue:detection,epidemiology,and differences from adults.J Pediatr,2011,159(6):939-944.
6. Virtanen KA,Nuutila P.Brown adipose tissue in humans.Curr Opin Lipidol,2011,22(1):49-54.
7. Zhang Q,Ma B,Fischman AJ,et al.Increased uncoupling protein 1 mRNA expression in mice brown adipose tissue after burn injury.J Burn Care Res,2008,29(2):358-362.
8. Bartelt A,Merkel M,Heeren J.A new,powerful player in lipoprotein metabolism:brown adipose tissue.J Mol Med (Berl),2012,90(8):887-893.
9. Kraft R,Kulp GA,Herndon DN,et al.Is there a difference in clinical outcomes,inflammation,and hypermetabolism between scald and flame burn? Pediatr Criti Care Med,2011,12(6):e275-281.
10. Pawlik TM,Carter EA,Bode BP,et al.Central role of interleukin-6 in burn induced stimulation of hepatic amino acid transport.Int J Mol Med,2003,12(4):541-548.
11. Zhang Q,Carter EA,Ma BY,et al.Molecular mechanism(s) of burn-induced insulin resistance in murine skeletal muscle:role of IRS phosphorylation.Life Sci,2005,77(24):3068-3077.
12. 郝岱峰,柴家科,吴焱秋.烫伤大鼠创面脓毒症发生后肝组织中脂代谢相关基因表达水平的变化.中华烧伤杂志,2006,22(4):250-253.
13. Izamis ML,Uygun K,Uygun B,et al.Effects of burn injury on markers of hypermetabolism in rats.J Burn Care Res,2009,30(6):993-1001.
14. 解伟光,汪仕良,黎鳌.中国人烧伤后能量消耗中三大营养素的分布.第三军医大学学报,1992,14 (4):319-321.
15. 柴家科,盛志勇.应重视严重烧伤脓毒症患者骨骼肌蛋白高分解代谢的研究.中华医学杂志,2005,85(41):2883-2885.
16. Bajzer M,Olivieri M,Haas MK,et al.Cannabinoid receptor 1 (CB1) antagonism enhances glucose utilisation and activates brown adipose tissue in diet-induced obese mice.Diabetologia,2011,54(12):3121-3131.
17. Chaves VE,Frasson D,Garófalo MA,et al.Increased glyceride-glycerol synthesis in liver and brown adipose tissue of rat:in-vivo contribution of glycolysis and glyceroneogenesis.Lipids,2012,47(8):773-780.
18. Merritt EK,Cross JM,Bamman MM.Inflammatory and protein metabolism signaling responses in human skeletal muscle after burn injury.J Burn Care Res,2012,33(2):291-297.
19. Carter EA,Paul KW,Barrow SA,et al.Previous burn injury predisposes mice to lipopolysaccharide-induced changes in glucose metabolism.J Burn Care Res,2012,33(5):683-689.
20. Yasuhara S,Kaneki M,Sugita H,et al.Adipocyte apoptosis after burn injury is associated with altered fat metabolism.J Burn Care Res,2006,27(3):367-376.
21. Leone TC,Lehman JJ,Finck BN,et al.PGC-1alpha deficiency causes multi-system energy metabolic derangements:muscle dysfunction,abnormal weight control and hepatic steatosis.PLoS Biol,2005,3(4):e101.
22. Wu J,Boström P,Sparks LM,et al.Beige adipocytes are a distinct type of thermogenic fat cell in mouse and human.Cell,2012,150(2):366-376.
23. Bartelt A,Bruns OT,Reimer R,et al.Brown adipose tissue activity controls triglyceride clearance.Nat Med,2011,17(2):200-205.
24. Chang Y,She ZG,Sakimura K,et al.Ablation of NG2 proteoglycan leads to deficits in brown fat function and to adult onset obesity.PLoS One,2012,7(1):e30637.
25. Timmons JA,Wennmalm K,Larsson O,et al.Myogenic gene expression signature establishes that brown and white adipocytes originate from distinct cell lineages.Proc Natl Acad Sci U S A,2007,104(11):4401-4406.
26. Cronin CG,Prakash P,Daniels GH,et al.Brown fat at PET/CT:correlation with patient characteristics.Radiology,2012,263(3):836-842.
27. Kajimura S,Seale P,Kubota K,et al.Initiation of myoblast to brown fat switch by a PRDM16-C/EBP-beta transcriptional complex.Nature,2009,460(7259):1154-1158.
28. Cypess AM,Kahn CR.Brown fat as a therapy for obesity and diabetes.Curr Opin Endocrinol Diabetes Obes,2010,17(2):143-149.
29. Farmer SR.Molecular determinants of brown adipocyte formation and function.Genes Dev,2008,22(10):1269-1275.
30. Okita N,Hayashida Y,Kojima Y,et al.Differential responses of white adipose tissue and brown adipose tissue to caloric restriction in rats.Mech Ageing Dev,2012,133(5):255-266.
31. Sharon L,Jennifer H,Yuan X,et al.Cold-induced activation of brown adipose tissue and adipose angiogenesis in mice.Nature Protocols,2012,3(7):606-615.
32. Lim S,Honek J,Xue Y,et al.Cold-induced activation of brown adipose tissue and adipose angiogenesis in mice.Nat Protoc,2012,7(3):606-615.
33. Schulz TJ,Huang TL,Tran TT.Identification of inducible brown adipocyte progenitors residing in skeletal muscle and white fat.Proc Natl Acad Sci U S A,2011,108(1):143-148.

Chinese Journal of Reparative and Reconstructive Surgery

EXPERIMENTAL STUDY ON EFFECT OF BURN ON BROWN ADIPOSE TISSUE IN MICE

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