EXPERIMENTAL STUDY ON LONG-TERM PREVENTION EFFECT OF CHITOSAN ELECTROSPUN MEMBRANE ON CEREBROSPINAL FLUID LEAKAGE_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

GUOXingfeng ¹ ,  HOUChunlin ¹ , DOUYuandong ¹ , LINYe ² , LEIDeqiao ¹

1. Department of Orthopedic Surgery, Changzheng Hospital of the Second Military Medical University, Shanghai, 200003, P. R. China;
2. Institute of Nephrology, the Second Military Medical University, Shanghai, 200003, P. R. China;

Corresponding author：

HOUChunlin, Email: chunlin_hou@163.com

Keywords：

Chitosan electrospun membrane; Cerebrospinal fluid leakage; Dural defect; Long-term effect; Rabbit

DOI：

10.7507/1002-1892.20140219

Video：

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Objective To study the long-term prevention effect of self-developed chitosan electrospun membrane on cerebrospinal fluid leakage. Methods Twenty-five healthy adult New Zealand rabbits were selected to prepare the bilateral dural defect (0.8 cm×0.8 cm in size) via midline incision of head.Defect of the right was repaired with chitosan electrospun membrane as the experimental group; defect of the left was not repaired as the control group.At 2-16 weeks after operation,one rabbit was sacrificed for the general observation of inflammatory response surrounding bone window and absorption of chitosan electrospun membrane; at 3 and 6 weeks after operation,5 rabbits were sacrificed for sampling to observe histological change and collagen expression by HE and Masson staining,and to measure the expressions of epidermal growth factor receptor (EGFR) and basic fibroblast growth factor (bFGF) by immunohistochemical staining. Results No inflammatory reaction of swelling,exudation,and sppuration appeared in the skin and subcutaneous tissue after operation in 2 groups.There was no adhesion around the chitosan electrospun membrane,and new fiber membrane formed under the chitosan electrospun membrane in the experimental group; no cerebrospinal fluid leakage happened; the chitosan electrospun membrane was gradually degraded with time,and was completely absorbed at 16 weeks.There was uneven scar around the dural detect in control group.Histological observation showed less inflammatory cell infiltration in the experimental group,showing significant difference in the number of inflammatory cells compared with control group at 3,6 weeks (P<0.05); capillary,granulation tissue and collagen fiber massively proliferated; collagen fiber arranged in line,and there was a clear borderline between chitosan electrospun membrane and adjacent collagen fiber.The immunohistochemical staining showed that there were high expressions of bFGF and EGFR in the experimental group,and low expressions of bFGF and EGFR in the control group. Conclusion Chitosan electrospun membrane for dural defect of rabbit can effectively reconstruct the dura,and it has exact long-term prevention effect on cerebrospinal fluid leakage.

Citation： GUOXingfeng, HOUChunlin, DOUYuandong, LINYe, LEIDeqiao. EXPERIMENTAL STUDY ON LONG-TERM PREVENTION EFFECT OF CHITOSAN ELECTROSPUN MEMBRANE ON CEREBROSPINAL FLUID LEAKAGE. Chinese Journal of Reparative and Reconstructive Surgery, 2014, 28(8): 993-997. doi: 10.7507/1002-1892.20140219 Copy

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2.	Kim DW,Eum WS,Jang SH,et al.A transparent artificial dura mater made of silk fibroin as an inhibitor of inflammation in craniotomized rats.J Neurosurg,2011,114(2):485-490.
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6.	肖海军,侯春林,顾其胜,等.几丁糖膜预防术后粘连的研究进展.上海生物医学工程,2004,25(3):47-52.
7.	Xu C,Lei C,Meng L,et al.Chitosan as a barrier membrane material in periodontal tissue regeneration.J Biomed Mater Res Part B Appl Biomater,2012,100(5):1435-1443.
8.	郭兴锋,窦源东,侯春林,等.几丁糖电纺膜封堵脑脊液漏即刻效果的实验研究.生物骨科材料与临床研究,2014,11(4):1-4.
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10.	Hurt AP,Getti G,Coleman NJ.Bioactivity and biocompatibility of a chitosan-tobermorite composite membrane for guided tissue regeneration.Int J Biol Macromol,2014,64:11-16.
11.	Hughes SA,Ozgur BM,German M,et al.Prolonged Jackson-Pratt drainage in the management of lumbar cerebrospinal fluid leaks.Surg Neurol,2006,65(4):410-415.
12.	Kievit FM,Cooper A,Jana S,et al.Aligned chitosan-polycaprolactone polyblend nanofibers promote the migration of glioblastoma cells.Adv Healthc Mater,2013,2(12):1651-1659.
13.	夏平光,侯春林,王万宏.几丁糖抑制人成纤维细胞增殖的实验研究.中国修复重建外科杂志,2007,21(8):833-836.
14.	Zhou Y,Yang H,Liu X,et al.Electrospinning of carboxyethyl chitosan/poly(vinyl alcohol)/silk fibroin nanoparticles for wound dressings.Inter J Biol Macromol,2013,53:88-92.
15.	Bai W,Wang X,Yuan W,et al.Application of PLGA/type I collagen/chitosan artificial composite dura mater in the treatment of dural injury.J Materials Sc Mater Med,2013,24(9):2247-2254.

1. Parlato C,di Nuzzo G,Luongo M,et al.Use of a collagen biomatrix (TissuDura) for dura repair:a long-term neuroradiological and neuropathological evaluation.Acta Neurochir (Wien),2011,153(1):142-147.
2. Kim DW,Eum WS,Jang SH,et al.A transparent artificial dura mater made of silk fibroin as an inhibitor of inflammation in craniotomized rats.J Neurosurg,2011,114(2):485-490.
3. Grigoryants V,Jane JA Jr,Lin KY.Salvage of a complicated myelomeningocele using collagen (Duragen) and dermal (Alloderm) matrix substitutes.Case report and review of the literature.Pediatr Neurosurg,2007,43(6):512-515.
4. 侯春林,卢建照,包聚良,等.几丁质生物学特性研究.中国修复重建外科杂志,1993,7(2):115-116.
5. Pogorielov M,Kalinkevich O,Gortinskaya E,et al.The experimental application of chitosan membrane for treating chemical burns of the skin.Georgian Med News,2014,(226):65-70.
6. 肖海军,侯春林,顾其胜,等.几丁糖膜预防术后粘连的研究进展.上海生物医学工程,2004,25(3):47-52.
7. Xu C,Lei C,Meng L,et al.Chitosan as a barrier membrane material in periodontal tissue regeneration.J Biomed Mater Res Part B Appl Biomater,2012,100(5):1435-1443.
8. 郭兴锋,窦源东,侯春林,等.几丁糖电纺膜封堵脑脊液漏即刻效果的实验研究.生物骨科材料与临床研究,2014,11(4):1-4.
9. Rosen CL,Steinberg GK,DeMonte F,et al.Results of the prospective,randomized,multicenter clinical trial evaluating a biosynthesized cellulose graft for repair of dural defects.Neurosurgery,2011,69(5):1093-1094.
10. Hurt AP,Getti G,Coleman NJ.Bioactivity and biocompatibility of a chitosan-tobermorite composite membrane for guided tissue regeneration.Int J Biol Macromol,2014,64:11-16.
11. Hughes SA,Ozgur BM,German M,et al.Prolonged Jackson-Pratt drainage in the management of lumbar cerebrospinal fluid leaks.Surg Neurol,2006,65(4):410-415.
12. Kievit FM,Cooper A,Jana S,et al.Aligned chitosan-polycaprolactone polyblend nanofibers promote the migration of glioblastoma cells.Adv Healthc Mater,2013,2(12):1651-1659.
13. 夏平光,侯春林,王万宏.几丁糖抑制人成纤维细胞增殖的实验研究.中国修复重建外科杂志,2007,21(8):833-836.
14. Zhou Y,Yang H,Liu X,et al.Electrospinning of carboxyethyl chitosan/poly(vinyl alcohol)/silk fibroin nanoparticles for wound dressings.Inter J Biol Macromol,2013,53:88-92.
15. Bai W,Wang X,Yuan W,et al.Application of PLGA/type I collagen/chitosan artificial composite dura mater in the treatment of dural injury.J Materials Sc Mater Med,2013,24(9):2247-2254.

Chinese Journal of Reparative and Reconstructive Surgery

EXPERIMENTAL STUDY ON LONG-TERM PREVENTION EFFECT OF CHITOSAN ELECTROSPUN MEMBRANE ON CEREBROSPINAL FLUID LEAKAGE

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