RESEARCH PROGRESS OF URINE-DERIVED STEM CELLS_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

CHENChunyuan ^1,2 , NIUXin ¹ ,  WANGYang ^1,2

1. Institute of Microsurgery on Extremities, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai, 200233, P. R. China;
2. Graduate School of Nanchang University;

Corresponding author：

WANGYang, Email: wangy63cn@126.com

Keywords：

Urine-derived stem cells; Isolation and culture; Biological character; Tissue engineering; Seed cell

DOI：

10.7507/1002-1892.20150078

Video：

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Objective To review the current progresses in purification strategies, biological characters, and the uses in tissue engineering of urine-derived stem cells (USCs). Methods Recent relevant publications on the USCs were extensively reviewed, analyzed, and summarized. Results USCs, usually isolated by adherence screening method, are a population of mesenchymal stem cells (MSCs)-like somatic stem cells possessing robust self-renew and multi-potential differentiation ability. Combined with using appropriate biomaterials and biological molecules, USCs can be used as a good cell source for tissue engineering. Conclusion An alluring prospect exists on the USCs-related research. Further studies are required to investigate the origin, individual differences, and the therapeutic values of USCs.

Citation： CHENChunyuan, NIUXin, WANGYang. RESEARCH PROGRESS OF URINE-DERIVED STEM CELLS. Chinese Journal of Reparative and Reconstructive Surgery, 2015, 29(3): 372-376. doi: 10.7507/1002-1892.20150078 Copy

1.	Colombo JS, Carley A, Fleming GJ, et al. Osteogenic potential of bone marrow stromal cells on smooth, roughened, and tricalcium phosphate-modified titanium alloy surfaces. Int J Oral Maxillofac Implants, 2012, 27(5):1029-1042.
2.	Salomone R, Bento RF, Costa HJ, et al. Bone marrow stem cells in facial nerve regeneration from isolated stumps. Muscle Nerve, 2013, 48(3):423-429.
3.	Mohammadi R, Azizi S, Delirezh N, et al. The use of undifferentiated bone marrow stromal cells for sciatic nerve regeneration in rats. Int J Oral Maxillofac Surg, 2012, 41(5):650-656.
4.	Bharadwaj S, Liu G, Shi Y, et al. Multipotential differentiation of human urine-derived stem cells:potential for therapeutic applications in urology. Stem Cells, 2013, 31(9):1840-1856.
5.	Zhang Y, Mcneill E, Tian H, et al. Urine derived cells are a potential source for urological tissue reconstruction. J Urol, 2008, 180(5):2226-2233.
6.	汪泱, 关俊杰, 邓志锋, 等. 尿液间充质干细胞的提取及扩增培养方法和应用. 中国:CN201210470118.1. 2013-02-13.
7.	Atala A, Bauer SB, Soker S, et al. Tissue-engineered autologous bladders for patients needing cystoplasty. Lancet, 2006, 367(9518):1241-1246.
8.	Lang R, Liu G, Shi Y, et al. Self-renewal and differentiation capacity of urine-derived stem cells after urine preservation for 24 hours. PLoS One, 2013, 8(1):e53980.
9.	Bharadwaj S, Liu G, Shi Y, et al. Characterization of urine-derived stem cells obtained from upper urinary tract for use in cell-based urological tissue engineering. Tissue Eng Part A, 2011, 17(15-16):2123-2132.
10.	Chun SY, Kim HT, Lee JS, et al. Characterization of urine-derived cells from upper urinary tract in patients with bladder cancer. Urology, 2012, 79(5):1186.e1-7.
11.	Sutherland GR, Bain AD. Culture of cells from the urine of newborn children. Nature, 1972, 239(5369):231.
12.	Crandall L, Lalande M. Is urine the next source of stem cells? Regen Med, 2013, 8(3):235-236.
13.	Zhang D, Wei G, Li P, et al. Urine-derived stem cells:A novel and versatile progenitor source for cell-based therapy and regenerative medicine. Genes Dis, 2014, 1(1):8-17.
14.	Qin D, Long T, Deng J, et al. Urine-derived stem cells for potential use in bladder repair. Stem Cell Res Ther, 2014, 5(3):69.
15.	Racusen LC, Fivush BA, Andersson H, et al. Culture of renal tubular cells from the urine of patients with nephropathic cystinosis. J Am Soc Nephrol, 1991, 1(8):1028-1033.
16.	Guan JJ, Niu X, Gong FX, et al. Biological characteristics of human-urine-derived stem cells:potential for cell-based therapy in neurology. Tissue Eng Part A, 2014, 20(13-14):1794-1806.
17.	Benda C, Zhou T, Wang X, et al. Urine as a source of stem cells. Adv Biochem Eng Biotechnol, 2013, 129:19-32.
18.	Wu S, Wang Z, Bharadwaj S, et al. Implantation of autologous urine derived stem cells expressing vascular endothelial growth factor for potential use in genitourinary reconstruction. J Urol, 2011, 186(2):640-647.
19.	赵阳, 张明, 王忠, 等. 组织工程支架材料在膀胱修复中的应用进展. 组织工程与重建外科杂志, 2013, 9(5):289-291.
20.	Wu S, Liu Y, Bharadwaj S, et al. Human urine-derived stem cells seeded in a modified 3D porous small intestinal submucosa scaffold for urethral tissue engineering. Biomaterials, 2011, 32(5):1317-1326.
21.	Liu G, Wang X, Sun X, et al. The effect of urine-derived stem cells expressing VEGF loaded in collagen hydrogels on myogenesis and innervation following after subcutaneous implantation in nude mice. Biomaterials, 2013, 34(34):8617-8629.
22.	Liu G, Pareta RA, Wu R, et al. Skeletal myogenic differentiation of urine-derived stem cells and angiogenesis using microbeads loaded with growth factors. Biomaterials, 2013, 34(4):1311-1326.
23.	Pei M, Li J, Zhang Y, et al. Expansion on a matrix deposited by nonchondrogenic urine stem cells strengthens the chondrogenic capacity of repeated-passage bone marrow stromal cells. Cell Tissue Res, 2014, 356(2):391-403.
24.	Lin HK, Godiwalla SY, Palmer B, et al. Understanding roles of porcine small intestinal submucosa in urinary bladder regeneration:identification of variable regenerative characteristics of small intestinal submucosa. Tissue Eng Part B Rev, 2014, 20(1):73-83.
25.	Luo JC, Chen W, Chen XH, et al. A multi-step method for preparation of porcine small intestinal submucosa (SIS). Biomaterials, 2011, 32(3):706-713.
26.	Zhang Y, Kropp BP, Lin HK, et al. Bladder regeneration with cell-seeded small intestinal submucosa. Tissue Eng, 2004, 10(1-2):181-187.
27.	Bodin A, Bharadwaj S, Wu S, et al. Tissue-engineered conduit using urine-derived stem cells seeded bacterial cellulose polymer in urinary reconstruction and diversion. Biomaterials, 2010, 31(34):8889-8901.
28.	张帅, 赵维明, 修有成. 压力性尿失禁干细胞治疗的新进展. 中华临床医师杂志(电子版), 2013, 7(22):10290-10293.
29.	李颖, 董武. 干细胞治疗女性压力性尿失禁研究进展. 中国实用妇科与产科杂志, 2011, 27(5):398-400.
30.	Deasy BM, Feduska JM, Payne TR, et al. Effect of VEGF on the regenerative capacity of muscle stem cells in dystrophic skeletal muscle. Mol Ther, 2009, 17(10):1788-1798.
31.	Gnavi S, di Blasio L, Tonda-Turo C, et al. Gelatin-based hydrogel for vascular endothelial growth factor release in peripheral nerve tissue engineering. J Tissue Eng Regen Med, 2014.[Epub ahead of print].
32.	Nikolaev SI, Gallyamov AR, Mamin GV, et al. Poly(epsilon-caprolactone) nerve conduit and local delivery of vegf and fgf2 genes stimulate neuroregeneration. Bull Exp Biol Med, 2014, 157(1):155-158.
33.	Hou S, Xu Q, Tian W, et al. The repair of brain lesion by implantation of hyaluronic acid hydrogels modified with laminin. J Neurosci Methods, 2005, 148(1):60-70.
34.	Fu Y, Guan J, Guo S, et al. Human urine-derived stem cells in combination with polycaprolactone/gelatin nanofibrous membranes enhance wound healing by promoting angiogenesis. J Transl Med,.

1. Colombo JS, Carley A, Fleming GJ, et al. Osteogenic potential of bone marrow stromal cells on smooth, roughened, and tricalcium phosphate-modified titanium alloy surfaces. Int J Oral Maxillofac Implants, 2012, 27(5):1029-1042.
2. Salomone R, Bento RF, Costa HJ, et al. Bone marrow stem cells in facial nerve regeneration from isolated stumps. Muscle Nerve, 2013, 48(3):423-429.
3. Mohammadi R, Azizi S, Delirezh N, et al. The use of undifferentiated bone marrow stromal cells for sciatic nerve regeneration in rats. Int J Oral Maxillofac Surg, 2012, 41(5):650-656.
4. Bharadwaj S, Liu G, Shi Y, et al. Multipotential differentiation of human urine-derived stem cells:potential for therapeutic applications in urology. Stem Cells, 2013, 31(9):1840-1856.
5. Zhang Y, Mcneill E, Tian H, et al. Urine derived cells are a potential source for urological tissue reconstruction. J Urol, 2008, 180(5):2226-2233.
6. 汪泱, 关俊杰, 邓志锋, 等. 尿液间充质干细胞的提取及扩增培养方法和应用. 中国:CN201210470118.1. 2013-02-13.
7. Atala A, Bauer SB, Soker S, et al. Tissue-engineered autologous bladders for patients needing cystoplasty. Lancet, 2006, 367(9518):1241-1246.
8. Lang R, Liu G, Shi Y, et al. Self-renewal and differentiation capacity of urine-derived stem cells after urine preservation for 24 hours. PLoS One, 2013, 8(1):e53980.
9. Bharadwaj S, Liu G, Shi Y, et al. Characterization of urine-derived stem cells obtained from upper urinary tract for use in cell-based urological tissue engineering. Tissue Eng Part A, 2011, 17(15-16):2123-2132.
10. Chun SY, Kim HT, Lee JS, et al. Characterization of urine-derived cells from upper urinary tract in patients with bladder cancer. Urology, 2012, 79(5):1186.e1-7.
11. Sutherland GR, Bain AD. Culture of cells from the urine of newborn children. Nature, 1972, 239(5369):231.
12. Crandall L, Lalande M. Is urine the next source of stem cells? Regen Med, 2013, 8(3):235-236.
13. Zhang D, Wei G, Li P, et al. Urine-derived stem cells:A novel and versatile progenitor source for cell-based therapy and regenerative medicine. Genes Dis, 2014, 1(1):8-17.
14. Qin D, Long T, Deng J, et al. Urine-derived stem cells for potential use in bladder repair. Stem Cell Res Ther, 2014, 5(3):69.
15. Racusen LC, Fivush BA, Andersson H, et al. Culture of renal tubular cells from the urine of patients with nephropathic cystinosis. J Am Soc Nephrol, 1991, 1(8):1028-1033.
16. Guan JJ, Niu X, Gong FX, et al. Biological characteristics of human-urine-derived stem cells:potential for cell-based therapy in neurology. Tissue Eng Part A, 2014, 20(13-14):1794-1806.
17. Benda C, Zhou T, Wang X, et al. Urine as a source of stem cells. Adv Biochem Eng Biotechnol, 2013, 129:19-32.
18. Wu S, Wang Z, Bharadwaj S, et al. Implantation of autologous urine derived stem cells expressing vascular endothelial growth factor for potential use in genitourinary reconstruction. J Urol, 2011, 186(2):640-647.
19. 赵阳, 张明, 王忠, 等. 组织工程支架材料在膀胱修复中的应用进展. 组织工程与重建外科杂志, 2013, 9(5):289-291.
20. Wu S, Liu Y, Bharadwaj S, et al. Human urine-derived stem cells seeded in a modified 3D porous small intestinal submucosa scaffold for urethral tissue engineering. Biomaterials, 2011, 32(5):1317-1326.
21. Liu G, Wang X, Sun X, et al. The effect of urine-derived stem cells expressing VEGF loaded in collagen hydrogels on myogenesis and innervation following after subcutaneous implantation in nude mice. Biomaterials, 2013, 34(34):8617-8629.
22. Liu G, Pareta RA, Wu R, et al. Skeletal myogenic differentiation of urine-derived stem cells and angiogenesis using microbeads loaded with growth factors. Biomaterials, 2013, 34(4):1311-1326.
23. Pei M, Li J, Zhang Y, et al. Expansion on a matrix deposited by nonchondrogenic urine stem cells strengthens the chondrogenic capacity of repeated-passage bone marrow stromal cells. Cell Tissue Res, 2014, 356(2):391-403.
24. Lin HK, Godiwalla SY, Palmer B, et al. Understanding roles of porcine small intestinal submucosa in urinary bladder regeneration:identification of variable regenerative characteristics of small intestinal submucosa. Tissue Eng Part B Rev, 2014, 20(1):73-83.
25. Luo JC, Chen W, Chen XH, et al. A multi-step method for preparation of porcine small intestinal submucosa (SIS). Biomaterials, 2011, 32(3):706-713.
26. Zhang Y, Kropp BP, Lin HK, et al. Bladder regeneration with cell-seeded small intestinal submucosa. Tissue Eng, 2004, 10(1-2):181-187.
27. Bodin A, Bharadwaj S, Wu S, et al. Tissue-engineered conduit using urine-derived stem cells seeded bacterial cellulose polymer in urinary reconstruction and diversion. Biomaterials, 2010, 31(34):8889-8901.
28. 张帅, 赵维明, 修有成. 压力性尿失禁干细胞治疗的新进展. 中华临床医师杂志(电子版), 2013, 7(22):10290-10293.
29. 李颖, 董武. 干细胞治疗女性压力性尿失禁研究进展. 中国实用妇科与产科杂志, 2011, 27(5):398-400.
30. Deasy BM, Feduska JM, Payne TR, et al. Effect of VEGF on the regenerative capacity of muscle stem cells in dystrophic skeletal muscle. Mol Ther, 2009, 17(10):1788-1798.
31. Gnavi S, di Blasio L, Tonda-Turo C, et al. Gelatin-based hydrogel for vascular endothelial growth factor release in peripheral nerve tissue engineering. J Tissue Eng Regen Med, 2014.[Epub ahead of print].
32. Nikolaev SI, Gallyamov AR, Mamin GV, et al. Poly(epsilon-caprolactone) nerve conduit and local delivery of vegf and fgf2 genes stimulate neuroregeneration. Bull Exp Biol Med, 2014, 157(1):155-158.
33. Hou S, Xu Q, Tian W, et al. The repair of brain lesion by implantation of hyaluronic acid hydrogels modified with laminin. J Neurosci Methods, 2005, 148(1):60-70.
34. Fu Y, Guan J, Guo S, et al. Human urine-derived stem cells in combination with polycaprolactone/gelatin nanofibrous membranes enhance wound healing by promoting angiogenesis. J Transl Med,.

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