STUDY ON MODIFICATION OF BIOMATERIALS OF ACELLULAR BOVINE PERICARDIUM WITH DIFFERENT CROSSLINKING REAGENTS_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

 XIAOHongtao , TIANShemin , ZHAXinjian , WEIYing , HUANGHongjun , LIYun , YANGHuanna , XIAChengde , NIUXihua

Department of Burns, No. 1 People's Hospital of Zhengzhou, Zhengzhou Henan, 450004, P. R. China;

Corresponding author：

XIAOHongtao, Email: htxiao917@126.com

Keywords：

Acellular bovine pericardium; Crosslinking reagent; Genipin; 1-ethyl-3-(3-dinethylami-nopropyl)carbodimide; Biological property

DOI：

10.7507/1002-1892.20150281

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the effects of modification of acellular bovine pericardium with 1-ethyl-3-(3-dinethylami-nopropyl) carbodimide (EDC)/N-hydroxysuccininide (NHS) or genipin and find out the best crosslinking reagent. Methods The cellular components of the bovine pericardiums were removed. The effects of decellularization were tested by HE staining. The acellular bovine pericardiums were crosslinked with EDC/NHS (EDC/NHS group) or genipin (genipin group). The properties of the crosslinked acellular matrix were evaluated by scanning electron microscope (SEM), matrix thickness, crosslinking index, mechanical property, denaturation temperature, enzymatic degradation, and cytotoxicity test before and after the crosslinking. Acellular bovine pericardium (ABP group) or normal bovine pericardium (control group) were harvested as controls. Results SEM showed that collagen fibers were reticulated in bovine pericardial tissues after crosslinked by EDC/NHS or genipin, and relative aperture of the collagen fiber was from 10 to 20 μm. The thickness and denaturation temperature of the scaffolds were increased significantly after crosslinking with EDC/NHS or genipin (P<0.05), while there was no significant difference between EDC/NHS group and genipin group (P>0.05). The difference had no statistical significance in crosslinking index between EDC/NHS group and genipin group (t=0.205, P=0.218). The degradation rate in EDC/NHS group and genipin group was significantly lower than that in ABP group and control group (P<0.05). Elastic modulus and fracture stress in EDC/NHS group and genipin group were significantly lower than those in ABP group (P<0.05), but there was no significant difference among EDC/NHS group, genipin group, and control group (P>0.05). The break elongation in EDC/NHS group and genipin group were significantly increased than those in ABP group and control group (P<0.05). The difference had no statistical significance in stability and mechanical properties between EDC/NHS group and genipin group (P>0.05). Cytotoxicity of genipin crosslinked tissue (grade 1) were much lower than that of EDC/NHS (grade 2) at 5 days. Conclusion Acellular bovine pericardium crosslinked with genipin has better biocompatibility than EDC/NHS.

Citation： XIAOHongtao, TIANShemin, ZHAXinjian, WEIYing, HUANGHongjun, LIYun, YANGHuanna, XIAChengde, NIUXihua. STUDY ON MODIFICATION OF BIOMATERIALS OF ACELLULAR BOVINE PERICARDIUM WITH DIFFERENT CROSSLINKING REAGENTS. Chinese Journal of Reparative and Reconstructive Surgery, 2015, 29(10): 1301-1306. doi: 10.7507/1002-1892.20150281 Copy

1.	Strange G, Brizard C, Karl TR, et al. An evaluation of Admedus' tissue engineering process-treated (ADAPT) bovine pericardium patch (CardioCel) for the repair of cardiac and vascular defects. Expert Rev Med Devices, 2015, 12(2):135-141.
2.	Dorweiler B, Kayser C, Zipp F, et al. Long-term performance of the bovine pericardium patch in conventional carotid endarterectomy. Thorac Cardiovasc Surg, 2015, 63(2):168-174.
3.	Gubitosi A, Docimo G, Parmeggiani D, et al. Acellular bovine pericardium dermal matrix in immediate breast reconstruction after Skin Sparing Mastectomy. Int J Surg, 2014, 12 Suppl 1:S205-208.
4.	Leon-Takahashi AM, Gomez-Pedraza A, Cruz-Rodriguez A, et al. Use of glutaraldehyde-preserved bovine pericardium patch in vascular repair of portal vein in a patient with pancreatic cancer. Indian J Surg, 2013, 75(Suppl 1):247-249.
5.	Barros JA, Filippin-Monteiro FB, de Oliveira EM, et al. Cytotoxicity of PVPAC-treated bovine pericardium:a potential replacement for glutaraldehyde in biological heart valves. J Biomed Mater Res B Appl Biomater, 2014, 102(3):574-582.
6.	Shepherd DV, Shepherd JH, Ghose S, et al. The process of EDCNHS cross-linking of reconstituted collagen fibres increases collagen fibrillar order and alignment. APL Mater, 2015, 3(1):014902.
7.	王旻, 笪琳萃, 谢艳, 等. 京尼平作为交联剂在天然生物材料改性中的应用. 中国修复重建外科杂志, 2013, 27(5):580-585.
8.	Maizato MJ, Higa OZ, Mathor MB, et al. Glutaraldehyde-treated bovine pericardium:effects of lyophilization on cytotoxicity and residual aldehydes. Artif Organs, 2003, 27(8):692-694.
9.	Chang Y, Tsai CC, Liang HC, et al. In vivo evaluation of cellular and acellular bovine pericardia fixed with a naturally occurring crosslinking agent (genipin). Biomaterials, 2002, 23(12):2447-2457.
10.	董小超. RGD交联去细胞牛心包构建组织工程心脏瓣膜支架的实验研究. 西安:第四军医大学, 2009.
11.	王迎军, 杨春蓉, 汪凌云. EDC/NHS交联对胶原物理化学性能的影响. 华南理工大学学报:自然科学版, 2007, 35(12):66-70, 96.
12.	姚芳莲, 李学强, 于潇, 等. 京尼平对壳聚糖及明胶的交联反应. 天津大学学报, 2007, 40(12):1485-1489.
13.	马兵, 翟万银, 张嘉敏, 等. PC交联脱细胞牛心包膜的力学性能、稳定性和血液相容性. 华东师范大学学报:自然科学版, 2013, 59(5):61-70, 79.
14.	吴彤, 王瑞明, 黄磊, 等. 蛋白酶K的研究进展. 食品工业科技, 2013, 34(14):380-384.
15.	陈平, 李新华, 邢万红. 京尼平交联的脱细胞牛心包生物支架材料的实验研究. 中国医药导报, 2010, 7(6):28-30.
16.	Brizard CP, Brink J, Horton SB, et al. New engineering treatment of bovine pericardium confers outstanding resistance to calcification in mitral and pulmonary implantations in a juvenile sheep model. J Thorac Cardiovasc Surg, 2014, 148(6):3194-3201.
17.	Scheffel D, Bianchi L, Soares D, et al. Transdentinal cytotoxicity of carbodiimide (EDC) and glutaraldehyde on odontoblast-like cells. Oper Dent, 2015, 40(1):44-54.
18.	田聪, 万荣欣, 刘欣, 等. 生物交联剂京尼平交联牛心包生物支架材料的性能. 中国生物医学工程学报, 2011, 30(2):281-286.
19.	汪红, 苏建, 高陪, 等. 不同pH值对京尼平交联胶原制备生物材料的影响. 四川大学学报:自然科学版, 2011, 48(2):481-485.
20.	曹峥, 胡蕰玉, 潘纬敏, 等. 交联温度对京尼平交联胶原/壳聚糖组织工程支架的影响. 中国矫形外科杂志, 2009, 17(3):217-220.
21.	Gratzer PF, Lee JM. Control of pH alters the type of cross-linking produced by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) treatment of acellular matrix vascular grafts. J Biomed Mater Res, 2001, 58(2):172-179.
22.	吴秀娟, 谢明均. 京尼平对去细胞组织修饰的研究进展. 中华临床医师杂志(电子版), 2014, 8(13):2532-2535.
23.	秦胜男, 陈鸿辉, 杨小红, 等. 经碳化二胺/N-羟基琥珀酰亚胺交联的Ⅱ型胶原海绵的制备. 中国矫形外科杂志, 2010, 18(12):1014-1018.

1. Strange G, Brizard C, Karl TR, et al. An evaluation of Admedus' tissue engineering process-treated (ADAPT) bovine pericardium patch (CardioCel) for the repair of cardiac and vascular defects. Expert Rev Med Devices, 2015, 12(2):135-141.
2. Dorweiler B, Kayser C, Zipp F, et al. Long-term performance of the bovine pericardium patch in conventional carotid endarterectomy. Thorac Cardiovasc Surg, 2015, 63(2):168-174.
3. Gubitosi A, Docimo G, Parmeggiani D, et al. Acellular bovine pericardium dermal matrix in immediate breast reconstruction after Skin Sparing Mastectomy. Int J Surg, 2014, 12 Suppl 1:S205-208.
4. Leon-Takahashi AM, Gomez-Pedraza A, Cruz-Rodriguez A, et al. Use of glutaraldehyde-preserved bovine pericardium patch in vascular repair of portal vein in a patient with pancreatic cancer. Indian J Surg, 2013, 75(Suppl 1):247-249.
5. Barros JA, Filippin-Monteiro FB, de Oliveira EM, et al. Cytotoxicity of PVPAC-treated bovine pericardium:a potential replacement for glutaraldehyde in biological heart valves. J Biomed Mater Res B Appl Biomater, 2014, 102(3):574-582.
6. Shepherd DV, Shepherd JH, Ghose S, et al. The process of EDCNHS cross-linking of reconstituted collagen fibres increases collagen fibrillar order and alignment. APL Mater, 2015, 3(1):014902.
7. 王旻, 笪琳萃, 谢艳, 等. 京尼平作为交联剂在天然生物材料改性中的应用. 中国修复重建外科杂志, 2013, 27(5):580-585.
8. Maizato MJ, Higa OZ, Mathor MB, et al. Glutaraldehyde-treated bovine pericardium:effects of lyophilization on cytotoxicity and residual aldehydes. Artif Organs, 2003, 27(8):692-694.
9. Chang Y, Tsai CC, Liang HC, et al. In vivo evaluation of cellular and acellular bovine pericardia fixed with a naturally occurring crosslinking agent (genipin). Biomaterials, 2002, 23(12):2447-2457.
10. 董小超. RGD交联去细胞牛心包构建组织工程心脏瓣膜支架的实验研究. 西安:第四军医大学, 2009.
11. 王迎军, 杨春蓉, 汪凌云. EDC/NHS交联对胶原物理化学性能的影响. 华南理工大学学报:自然科学版, 2007, 35(12):66-70, 96.
12. 姚芳莲, 李学强, 于潇, 等. 京尼平对壳聚糖及明胶的交联反应. 天津大学学报, 2007, 40(12):1485-1489.
13. 马兵, 翟万银, 张嘉敏, 等. PC交联脱细胞牛心包膜的力学性能、稳定性和血液相容性. 华东师范大学学报:自然科学版, 2013, 59(5):61-70, 79.
14. 吴彤, 王瑞明, 黄磊, 等. 蛋白酶K的研究进展. 食品工业科技, 2013, 34(14):380-384.
15. 陈平, 李新华, 邢万红. 京尼平交联的脱细胞牛心包生物支架材料的实验研究. 中国医药导报, 2010, 7(6):28-30.
16. Brizard CP, Brink J, Horton SB, et al. New engineering treatment of bovine pericardium confers outstanding resistance to calcification in mitral and pulmonary implantations in a juvenile sheep model. J Thorac Cardiovasc Surg, 2014, 148(6):3194-3201.
17. Scheffel D, Bianchi L, Soares D, et al. Transdentinal cytotoxicity of carbodiimide (EDC) and glutaraldehyde on odontoblast-like cells. Oper Dent, 2015, 40(1):44-54.
18. 田聪, 万荣欣, 刘欣, 等. 生物交联剂京尼平交联牛心包生物支架材料的性能. 中国生物医学工程学报, 2011, 30(2):281-286.
19. 汪红, 苏建, 高陪, 等. 不同pH值对京尼平交联胶原制备生物材料的影响. 四川大学学报:自然科学版, 2011, 48(2):481-485.
20. 曹峥, 胡蕰玉, 潘纬敏, 等. 交联温度对京尼平交联胶原/壳聚糖组织工程支架的影响. 中国矫形外科杂志, 2009, 17(3):217-220.
21. Gratzer PF, Lee JM. Control of pH alters the type of cross-linking produced by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) treatment of acellular matrix vascular grafts. J Biomed Mater Res, 2001, 58(2):172-179.
22. 吴秀娟, 谢明均. 京尼平对去细胞组织修饰的研究进展. 中华临床医师杂志(电子版), 2014, 8(13):2532-2535.
23. 秦胜男, 陈鸿辉, 杨小红, 等. 经碳化二胺/N-羟基琥珀酰亚胺交联的Ⅱ型胶原海绵的制备. 中国矫形外科杂志, 2010, 18(12):1014-1018.

Chinese Journal of Reparative and Reconstructive Surgery

STUDY ON MODIFICATION OF BIOMATERIALS OF ACELLULAR BOVINE PERICARDIUM WITH DIFFERENT CROSSLINKING REAGENTS

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content