EFFECT OF SIMULATED IN VIVO PHYSIOLOGICAL ENVIRONMENT SEVERED LIMB FOSTERING SYSTEM APPLYING REMOTE ISCHEMIC CONDITIONING PERFUSION ON PRESERVING SEVERED LIMB_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

LITengfei ¹ ,  WANGJiangning ² , GAOLei ² , YINYefeng ²

1. Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, P. R. China;
2. Department of Plastic and Reconstructive Surgery, Beijing Shijitan Hospital, Capital Medical University;

Corresponding author：

WANGJiangning, Email: wangjiangning1234@163.com

Keywords：

Simulated in vivo physiological environment severed limb fostering system; Remote ischemic conditioning; Ischemia/reperfusion-induced injury; Pig

DOI：

10.7507/1002-1892.20160019

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the effect of simulated in vivo physiological environment severed limb fostering system applying remote ischemic conditioning (RIC) perfusion on preserving severed limb. Method Eighteen adult Bama mini pigs (24-30 kg in weight) were randomly divided into 3 groups (n=6) . No ischemic treatment was given in group A as normal control group; the right lower limbs were completely amputated and preserved at room temperature for 3 hours to make ischemic models in groups B and C, and then the severed limbs were put into the simulated in vivo physiological environment severed limb fostering system. Continuous blood perfusion was performed in group B, and continuous blood perfusion was performed after RIC perfusion in group C. After 8 hours of perfusion, the skeletal muscle samples were harvested for the morphology observation by transmission electron microscopy. The protein levels of B-cell lymphoma-2(Bcl-2) and Caspase-3 were detected by Western blot. The content of cytochrome C in both mitochondria and cytoplasm was determined by ELISA. Results Transmission electron microscopy results illustrated that the muscle fibers arranged more orderly and the mitochondria swelling was slighter in group C than group B. Western blot analysis showed that the protein levels of Bcl-2 and Caspase-3 were significantly higher in groups B and C than group A (P<0.05) ; the protein level of Bcl-2 significantly increased and the protein level of Caspase-3 significantly decreased in group C when compared with those in group B (P<0.05) . ELISA detection implicated that mitochondrial cytochrome C significantly reduced and cytosolic cytochrome C significantly increased in group B when compared with those in groups A and C (P<0.05) , but no significant difference was found between group A and group C (P>0.05) . Conclusions The ischemia/reperfusion-induced injury to skeletal muscle could be considerably inhibited by RIC perfusion. The simulated in vivo physiological environment severed limb fostering system applying RIC perfusion can significantly prolong the severed limb preserving time.

Citation： LITengfei, WANGJiangning, GAOLei, YINYefeng. EFFECT OF SIMULATED IN VIVO PHYSIOLOGICAL ENVIRONMENT SEVERED LIMB FOSTERING SYSTEM APPLYING REMOTE ISCHEMIC CONDITIONING PERFUSION ON PRESERVING SEVERED LIMB. Chinese Journal of Reparative and Reconstructive Surgery, 2016, 30(1): 91-94. doi: 10.7507/1002-1892.20160019 Copy

1.	Wang JN, Wang SY, Wang ZJ, et al. Temporary ectopic implantation for salvage of amputated lower extremities:case reports. Microsurgery, 2005, 25(5) :385-389.
2.	王江宁,童致虹,张铁慧,等.暂时性异位断足寄养再回植术.中国修复重建外科杂志, 2003, 17(1) :46-49.
3.	王江宁,尹叶锋,高磊,等.不同氧体积分数气体对体外模拟体内生理环境寄养断肢系统血氧分压的影响.中国组织工程研究, 2012, 16(5) :855-858.
4.	Tengfei L, Jinagning W. Remote ischemic conditioning:a novel way to treat ischemia-related injury of limbs. Med Hypotheses, 2015, 84(5) :504-505.
5.	Kharbanda RK, Nielsen TT, Redington AN. Translation of remote ischaemic preconditioning into clinical practice. Lancet, 2009, 374(9700) :1557-1565.
6.	Kleinbongard P, Neuhauser M, Thielmann M, et al. Confounders of cardioprotection by remote ischemic preconditioning in patients undergoing coronary artery bypass grafting. Cardiology, 2016, 133(2) :128-133.
7.	Hausenloy DJ, Mwamure PK, Venugopal V, et al. Effect of remote ischaemic preconditioning on myocardial injury in patients undergoing coronary artery bypass graft surgery:a randomised controlled trial. Lancet, 2007, 370(9587) :575-579.
8.	Sharma R, Randhawa PK, Singh N, et al. Bradykinin in ischemic conditioning-induced tissue protection:Evidences and possible mechanisms. Eur J Pharmacol, 2015, 768:58-70.
9.	Barbosa TC, Machado AC, Braz ID, et al. Remote ischemic preconditioning delays fatigue development during handgrip exercise. Scandinavian Journal of Medicine & Science in Sports, 2015, 25(3) :365-364.
10.	Wang WZ, Baynosa RC, Zamboni WA. Therapeutic interventions against reperfusion injury in skeletal muscle. J Orthop Res, 2011, 171(1) :175-182.
11.	韦正超,金文涛,张峻峰.四肢血管损伤的手术治疗.中国修复重建外科杂志, 2002, 16(3) :191-192.
12.	Arumugam TV, Shiels IA, Woodruff TM, et al. The role of the complement system in ischemia-reperfusion injury. Shock, 2004, 21(5) :401-409.
13.	Bøtker HE, Schmidt MR. The potential for remote ischemic conditioning to improve outcomes in heart failure. Expert Rev Cardiovasc Ther, 2015, 13(11) :1173-1176.
14.	Aimo A, Borrelli C, Giannoni A, et al. Cardioprotection by remote ischemic conditioning:Mechanisms and clinical evidences. World J Cardiol, 2015, 7(10) :621-632.
15.	尹叶锋,王江宁,高磊,等.不同温度对体外模拟体内生理环境寄养断肢系统的影响研究.中国修复重建外科杂志, 2013, 27(1) :72-76.
16.	张绍春,秦新愿,左有为,等.脉络宁对体外模拟体内生理环境寄养断肢系统缺血/再灌注损伤模型的作用.中国组织工程研究, 2014, 18(36) :5825-5829.
17.	Meeprom A, Sompong W, Suantawee T, et al. Isoferulic acid prevents methylglyoxal-induced protein glycation and DNA damage by free radical scavenging activity. BMC Complement Altern Med, 2015, 15(1) :346.
18.	洪念国,田中伟,李涛,等.断肢再植缺血再灌注损伤的保护机制.河南科技大学学报:医学版, 2006, 24(3) :171-172.
19.	Yu W, Shen T, Liu BY, et al. Cardiac shock wave therapy attenuates H9c2 myoblast apoptosis by activating the AKT signal. Cell Physiol Biochem, 2014, 33(5) :1293-1303.
20.	Zhang G, Han M, Wang X, et al. GRP75 involves in retinal ganglion cell apoptosis after rat pptic nerve crush. J Mol Neurosci, 2015, 56(2) :422-430.
21.	Wei P, Zhiyu C, Xu T, et al. Antitumor effect and apoptosis induction of Alocasia cucullata (Lour.) G. Don in human gastric cancer cells in vitro and in vivo. BMC Complement Altern Med, 2015, 15:33.
22.	王洪刚,刘小林,李智勇. UW液动脉预灌洗对缺血肢体再灌注损伤的影响.中山大学学报:医学科学版, 2013, 34(6) :856-860.

1. Wang JN, Wang SY, Wang ZJ, et al. Temporary ectopic implantation for salvage of amputated lower extremities:case reports. Microsurgery, 2005, 25(5) :385-389.
2. 王江宁,童致虹,张铁慧,等.暂时性异位断足寄养再回植术.中国修复重建外科杂志, 2003, 17(1) :46-49.
3. 王江宁,尹叶锋,高磊,等.不同氧体积分数气体对体外模拟体内生理环境寄养断肢系统血氧分压的影响.中国组织工程研究, 2012, 16(5) :855-858.
4. Tengfei L, Jinagning W. Remote ischemic conditioning:a novel way to treat ischemia-related injury of limbs. Med Hypotheses, 2015, 84(5) :504-505.
5. Kharbanda RK, Nielsen TT, Redington AN. Translation of remote ischaemic preconditioning into clinical practice. Lancet, 2009, 374(9700) :1557-1565.
6. Kleinbongard P, Neuhauser M, Thielmann M, et al. Confounders of cardioprotection by remote ischemic preconditioning in patients undergoing coronary artery bypass grafting. Cardiology, 2016, 133(2) :128-133.
7. Hausenloy DJ, Mwamure PK, Venugopal V, et al. Effect of remote ischaemic preconditioning on myocardial injury in patients undergoing coronary artery bypass graft surgery:a randomised controlled trial. Lancet, 2007, 370(9587) :575-579.
8. Sharma R, Randhawa PK, Singh N, et al. Bradykinin in ischemic conditioning-induced tissue protection:Evidences and possible mechanisms. Eur J Pharmacol, 2015, 768:58-70.
9. Barbosa TC, Machado AC, Braz ID, et al. Remote ischemic preconditioning delays fatigue development during handgrip exercise. Scandinavian Journal of Medicine & Science in Sports, 2015, 25(3) :365-364.
10. Wang WZ, Baynosa RC, Zamboni WA. Therapeutic interventions against reperfusion injury in skeletal muscle. J Orthop Res, 2011, 171(1) :175-182.
11. 韦正超,金文涛,张峻峰.四肢血管损伤的手术治疗.中国修复重建外科杂志, 2002, 16(3) :191-192.
12. Arumugam TV, Shiels IA, Woodruff TM, et al. The role of the complement system in ischemia-reperfusion injury. Shock, 2004, 21(5) :401-409.
13. Bøtker HE, Schmidt MR. The potential for remote ischemic conditioning to improve outcomes in heart failure. Expert Rev Cardiovasc Ther, 2015, 13(11) :1173-1176.
14. Aimo A, Borrelli C, Giannoni A, et al. Cardioprotection by remote ischemic conditioning:Mechanisms and clinical evidences. World J Cardiol, 2015, 7(10) :621-632.
15. 尹叶锋,王江宁,高磊,等.不同温度对体外模拟体内生理环境寄养断肢系统的影响研究.中国修复重建外科杂志, 2013, 27(1) :72-76.
16. 张绍春,秦新愿,左有为,等.脉络宁对体外模拟体内生理环境寄养断肢系统缺血/再灌注损伤模型的作用.中国组织工程研究, 2014, 18(36) :5825-5829.
17. Meeprom A, Sompong W, Suantawee T, et al. Isoferulic acid prevents methylglyoxal-induced protein glycation and DNA damage by free radical scavenging activity. BMC Complement Altern Med, 2015, 15(1) :346.
18. 洪念国,田中伟,李涛,等.断肢再植缺血再灌注损伤的保护机制.河南科技大学学报:医学版, 2006, 24(3) :171-172.
19. Yu W, Shen T, Liu BY, et al. Cardiac shock wave therapy attenuates H9c2 myoblast apoptosis by activating the AKT signal. Cell Physiol Biochem, 2014, 33(5) :1293-1303.
20. Zhang G, Han M, Wang X, et al. GRP75 involves in retinal ganglion cell apoptosis after rat pptic nerve crush. J Mol Neurosci, 2015, 56(2) :422-430.
21. Wei P, Zhiyu C, Xu T, et al. Antitumor effect and apoptosis induction of Alocasia cucullata (Lour.) G. Don in human gastric cancer cells in vitro and in vivo. BMC Complement Altern Med, 2015, 15:33.
22. 王洪刚,刘小林,李智勇. UW液动脉预灌洗对缺血肢体再灌注损伤的影响.中山大学学报:医学科学版, 2013, 34(6) :856-860.

Chinese Journal of Reparative and Reconstructive Surgery

EFFECT OF SIMULATED IN VIVO PHYSIOLOGICAL ENVIRONMENT SEVERED LIMB FOSTERING SYSTEM APPLYING REMOTE ISCHEMIC CONDITIONING PERFUSION ON PRESERVING SEVERED LIMB

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