HEALING MODEL RESEARCH OF ROTATOR CUFF INJURY IN CANINE_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

YEWei , BAONirong ,  ZHAOJianning

Department of Orthopedics, Nanjing General Hospital of Nanjing Military Command of Chinese PLA, Affiliated Hospital of Nanjing Medical College of Southern Medical University, Nanjing Jiangsu, 210000, P. R. China;

Corresponding author：

ZHAOJianning, Email: zhaojianning.0207@163.com

Keywords：

Rotator cuff injury; Animal model; Autologous semitendinosus expansion; Damage repair; Immobilization; Canine

DOI：

10.7507/1002-1892.20160093

Video：

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Objective To compare the difference of rotator cuff healing between different types of injury and between different repair methods, and to explore the animal model to accurately simulate the restorative process after repair of rotator cuff injury. Method Twelve adult male beagle dogs (weighing, 10-15 kg) were divided into 3 groups (n=4) according to different processing methods:acute rotator cuff injury+Mason-Allen suture repair (group A), huge rotator cuff injury+Mason-Allen suture repair (group B), and huge rotator cuff injury+Mason-Allen combined with autogenous semitendinosus expansion suture repair (group C). The external fixation was used for immobilization after repair. After operation, the general situation of the animals was observed, and the infraspinatus tendon was harvested for gross observation at 6 weeks after operation. The biomechanical test of limit load and histological observation of tendon fibers were carried out. Results All the animals survived to the end of the experiment. All incisions healed well and no infection occurred. Gross observation showed more scar tissues at the end of infraspinatus muscle tendon than normal tendon in group A; no obvious tendon tissue was observed at the end of infraspinatus muscle tendon in group B; the infraspinatus muscle tendon was covered with some white scar tissue, but the tendon and the general direction could be observed in group C. The limit load of groups A, B, and C were (223.75±24.28) , (159.25±34.87) , and (233.25±14.24) N respectively, group B was significantly lower than groups A and C (P<0.05) , and no significant differnce was found between group A and group C (P>0.05) . Histological observation showed normal arrangement of tendon fibers in group A; tendon fibers arranged disorderly in group B and tendon cells were significantly less than those of group A; tendon fibers arranged in neat in group C and tendon cells were more than those of group B. Conclusions Canine autologous semitendinosus expansion repair of massive rotator cuff injury immobilization model can better simulate the clinical rotator cuff injury healing process, so it can be used as an ideal animal model for related research.

Citation： YEWei, BAONirong, ZHAOJianning. HEALING MODEL RESEARCH OF ROTATOR CUFF INJURY IN CANINE. Chinese Journal of Reparative and Reconstructive Surgery, 2016, 30(4): 461-465. doi: 10.7507/1002-1892.20160093 Copy

1.	Gilot GJ, Attia AK, Alvarez AM. Arthroscopic repair of rotator cuff tears using extracellular matrix graft. Arthrosc Tech, 2014, 3(4) :e487-e489.
2.	Wagenhäuser MU, Pietschmann MF, Docheva D, et al. Assessment of essential characteristics of two different scaffolds for tendon in situ regeneration. Knee Surg Sports Traumatol Arthrosc, 2015, 23(4) :1239-1246.
3.	van der Meijden OA, Wijdicks CA, Gaskill TR, et al. Biomechanical analysis of two tendon posterosuperior rotator cuff tear repairs:extended linked repairs and augmented repairs. Arthroscopy, 2013, 29(1) :37-45.
4.	Shea KP, Obopilwe E, Sperling JW, et al. A biomechanical analysis of gap formation and failure mechanics of a xenograft-reinforced rotator cuff repair in a cadaveric model. J Shoulder Elbow Surg, 2012, 21(8) :1072-1079.
5.	Beason DP, Connizzo BK, Dourte LM, et al. Fiber-aligned polymer scaffolds for rotator cuff repair in a rat model. J Shoulder Elbow Surg, 2012, 21(2) :245-250.
6.	Adams JE, Zobitz ME, Reach JS Jr, et al. Rotator cuff repair using an acellular dermal matrix graft:an in vivo study in a canine model. Arthroscopy, 2006, 22(7) :700-709.
7.	Schlegel TF, Hawkins RJ, Lewis CW, et al. An in vivo comparison of the modified Mason-Allen suture technique versus an inclined horizontal mattress suture technique with regard to tendon-to-bone healing:a biomechanical and histologic study in sheep. J Shoulder Elbow Surg, 2007, 16(1) :115-121.
8.	Nicholson GP, Breur GJ, Van Sickle D, et al. Evaluation of a cross-linked acellular porcine dermal patch for rotator cuff repair augmentation in an ovine model. J Shoulder Elbow Surg, 2007, 16(5 Suppl):S184-190.
9.	Killian ML, Cavinatto LM, Ward SR, et al. Chronic degeneration leads to poor healing of repaired massive rotator cuff tears in rats. Am J Sports Med, 2015, 43(10) :2401-2410.
10.	Plate JF, Bates CM, Mannava S, et al. Age-related degenerative functional, radiographic, and histological changes of the shoulder in nonhuman primates. J Shoulder Elbow Surg, 2013, 22(8) :1019-1029.
11.	Xu H, Sandor M, Qi S, et al. Implantation of a porcine acellular dermal graft in a primate model of rotator cuff repair. J Shoulder Elbow Surg, 2012, 21(21) :580-588.
12.	Derwin KA, Baker AR, Codsi MJ, et al. Assessment of the canine model of rotator cuff injury and repair. J Shoulder Elbow Surg, 2007, 16(5 Suppl):S140-S148.
13.	Uezono K, Ide J, Tokunaga T, et al. Effect of immobilization on rotator cuff reconstruction with acellular dermal matrix grafts in an animal model. J Shoulder Elbow Surg, 2013, 22(9) :1290-1297.
14.	Kukkonen J, Joukainen A, Lehtinen J, et al. Treatment of nontraumatic rotator cuff tears:a randomized controlled trial with two years of clinical and imaging follow-up. J Bone Joint Surg (Am), 2015, 97(21) :1729-1737.
15.	Moosmayer S, Lund G, Seljom US, et al. Tendon repair compared with physiotherapy in the treatment of rotator cuff tears:a randomized controlled study in 103 cases with a five-year follow-up. J Bone Joint Surg (Am), 2014, 96(18) :1504-1514.
16.	MacDonald P, McRae S, Leiter J, et al. Arthroscopic rotator cuff repair with and without acromioplasty in the treatment of full-thickness rotator cuff tears:a multicenter, randomized controlled trial. J Bone Joint Surg (Am), 2011, 93(21) :1953-1960.
17.	Carbonel I, Martinez AA, Calvo A, et al. Single-row versus double-row arthroscopic repair in the treatment of rotator cuff tears:a prospective randomized clinical study. Int Orthop, 2012, 36(9) :1877-1883.
18.	Malavolta EA, Gracitelli ME, Ferreira Neto AA, et al. Platelet-rich plasma in rotator cuff repair:a prospective randomized study. Am J Sports Med, 2014, 42(10) :2446-2454.
19.	Derwin KA, Badylak SF, Steinmann SP, et al. Extracellular matrix scaffold devices for rotator cuff repair. J Shoulder Elbow Surg, 2012, 19(3) :467-476.
20.	Ide J, Kikukawa K, Hirose J, et al. Reconstruction of large rotator-cuff tears with acellular dermal matrix grafts in rats. J Shoulder Elbow Surg, 2009, 18(2) :288-295.
21.	Derwin KA, Baker AR, Spragg RK, et al. Commercial extracellular matrix scaffolds for rotator cuff tendon repair. Biomechanical, biochemical, and cellular properties. J Bone Joint Surg (Am), 2006, 88(12) :2665-2672.

1. Gilot GJ, Attia AK, Alvarez AM. Arthroscopic repair of rotator cuff tears using extracellular matrix graft. Arthrosc Tech, 2014, 3(4) :e487-e489.
2. Wagenhäuser MU, Pietschmann MF, Docheva D, et al. Assessment of essential characteristics of two different scaffolds for tendon in situ regeneration. Knee Surg Sports Traumatol Arthrosc, 2015, 23(4) :1239-1246.
3. van der Meijden OA, Wijdicks CA, Gaskill TR, et al. Biomechanical analysis of two tendon posterosuperior rotator cuff tear repairs:extended linked repairs and augmented repairs. Arthroscopy, 2013, 29(1) :37-45.
4. Shea KP, Obopilwe E, Sperling JW, et al. A biomechanical analysis of gap formation and failure mechanics of a xenograft-reinforced rotator cuff repair in a cadaveric model. J Shoulder Elbow Surg, 2012, 21(8) :1072-1079.
5. Beason DP, Connizzo BK, Dourte LM, et al. Fiber-aligned polymer scaffolds for rotator cuff repair in a rat model. J Shoulder Elbow Surg, 2012, 21(2) :245-250.
6. Adams JE, Zobitz ME, Reach JS Jr, et al. Rotator cuff repair using an acellular dermal matrix graft:an in vivo study in a canine model. Arthroscopy, 2006, 22(7) :700-709.
7. Schlegel TF, Hawkins RJ, Lewis CW, et al. An in vivo comparison of the modified Mason-Allen suture technique versus an inclined horizontal mattress suture technique with regard to tendon-to-bone healing:a biomechanical and histologic study in sheep. J Shoulder Elbow Surg, 2007, 16(1) :115-121.
8. Nicholson GP, Breur GJ, Van Sickle D, et al. Evaluation of a cross-linked acellular porcine dermal patch for rotator cuff repair augmentation in an ovine model. J Shoulder Elbow Surg, 2007, 16(5 Suppl):S184-190.
9. Killian ML, Cavinatto LM, Ward SR, et al. Chronic degeneration leads to poor healing of repaired massive rotator cuff tears in rats. Am J Sports Med, 2015, 43(10) :2401-2410.
10. Plate JF, Bates CM, Mannava S, et al. Age-related degenerative functional, radiographic, and histological changes of the shoulder in nonhuman primates. J Shoulder Elbow Surg, 2013, 22(8) :1019-1029.
11. Xu H, Sandor M, Qi S, et al. Implantation of a porcine acellular dermal graft in a primate model of rotator cuff repair. J Shoulder Elbow Surg, 2012, 21(21) :580-588.
12. Derwin KA, Baker AR, Codsi MJ, et al. Assessment of the canine model of rotator cuff injury and repair. J Shoulder Elbow Surg, 2007, 16(5 Suppl):S140-S148.
13. Uezono K, Ide J, Tokunaga T, et al. Effect of immobilization on rotator cuff reconstruction with acellular dermal matrix grafts in an animal model. J Shoulder Elbow Surg, 2013, 22(9) :1290-1297.
14. Kukkonen J, Joukainen A, Lehtinen J, et al. Treatment of nontraumatic rotator cuff tears:a randomized controlled trial with two years of clinical and imaging follow-up. J Bone Joint Surg (Am), 2015, 97(21) :1729-1737.
15. Moosmayer S, Lund G, Seljom US, et al. Tendon repair compared with physiotherapy in the treatment of rotator cuff tears:a randomized controlled study in 103 cases with a five-year follow-up. J Bone Joint Surg (Am), 2014, 96(18) :1504-1514.
16. MacDonald P, McRae S, Leiter J, et al. Arthroscopic rotator cuff repair with and without acromioplasty in the treatment of full-thickness rotator cuff tears:a multicenter, randomized controlled trial. J Bone Joint Surg (Am), 2011, 93(21) :1953-1960.
17. Carbonel I, Martinez AA, Calvo A, et al. Single-row versus double-row arthroscopic repair in the treatment of rotator cuff tears:a prospective randomized clinical study. Int Orthop, 2012, 36(9) :1877-1883.
18. Malavolta EA, Gracitelli ME, Ferreira Neto AA, et al. Platelet-rich plasma in rotator cuff repair:a prospective randomized study. Am J Sports Med, 2014, 42(10) :2446-2454.
19. Derwin KA, Badylak SF, Steinmann SP, et al. Extracellular matrix scaffold devices for rotator cuff repair. J Shoulder Elbow Surg, 2012, 19(3) :467-476.
20. Ide J, Kikukawa K, Hirose J, et al. Reconstruction of large rotator-cuff tears with acellular dermal matrix grafts in rats. J Shoulder Elbow Surg, 2009, 18(2) :288-295.
21. Derwin KA, Baker AR, Spragg RK, et al. Commercial extracellular matrix scaffolds for rotator cuff tendon repair. Biomechanical, biochemical, and cellular properties. J Bone Joint Surg (Am), 2006, 88(12) :2665-2672.

Chinese Journal of Reparative and Reconstructive Surgery

HEALING MODEL RESEARCH OF ROTATOR CUFF INJURY IN CANINE

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