COMPARISON OF TWO ANIMAL MODELS WITH CARTILAGE DEFECT_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

DUANXin ¹ , CHENQiang ^2,3 , LILang ⁴ , XUZhao ¹ , FANZhaoxin ² , ZHAOXue ⁴ , ZHANGLinhao ⁴ ,  XIANGZhou ¹

1. Department of Orthopedics, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, P. R. China;
2. Sichuan Cord Blood Bank, Sichuan Neo-life Stem Cell Biotech Inc;
3. Institude of Blood Transfusion, Chinese Academy of Medical Science;
4. West China School of Medicine, Sichuan University;

Corresponding author：

XIANGZhou, Email: xiangzhou15@hotmail.com

Keywords：

Cartilage excavation; Osteoarthritis; Cartilage defect; Animal model; Beagle

DOI：

10.7507/1002-1892.20160250

Video：

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Objective To compare difference in the establishment of animal model of cartilage defect by resection of medial collateral ligament and meniscus and by cartilage excavation so as to provide a proper way for the choose of animal model preparation of catilage defect. Methods Ten healthy beagles, male or female, weighing 5.0-10.0 kg, were randomly divided into 3 groups. Resection of knee collateral ligament and meniscus was performed on 4 beagles of group A, cartilage excavation of knee-joints in 4 beagles of group B, and no treatment on 2 beagles of group C as controls. At 16 weeks after modeling, MRI, gross observation, HE staining, Safranin O staining, and toluidine blue staining were performed, and Osteoarthritis Research Society International (OARSI) score was recorded. Results MRI and histology observation showed no obvious cartilage defect in group A; obvious cartilage defects were observed in group B and gross observation showed dramatic dark red cartilage defects. OARSI score was significantly lower in group A (0.940±0.574) than group B (4.500±0.516) (t=18.461, P=0.000). Conclusion The cartilage excavation is better than resection of both meniscus and medial collateral ligament, which provides a good method of establishing an animal model of cartilage defect at 16 weeks after operation.

Citation： DUAN Xin, CHEN Qiang, LI Lang, XU Zhao, FAN Zhaoxin, ZHAO Xue, ZHANG Linhao, XIANG Zhou. COMPARISON OF TWO ANIMAL MODELS WITH CARTILAGE DEFECT. Chinese Journal of Reparative and Reconstructive Surgery, 2016, 30(10): 1220-1224. doi: 10.7507/1002-1892.20160250 Copy

1.	Tsuboguchi S, Matsui N, Taneda Y, et al. Arthroscopic findings of cartilage changes in knees with rheumatoid arthritis. Ryumachi, 1989, 29(2):110-117.
2.	Frisbie DD, Cross MW, McIlwraith CW. A comparative study of articular cartilage thickness in the stifle of animal species used in human pre-clinical studies compared to articular cartilage thickness in the human knee. Vet Comp Orthop Traumatol, 2006, 19(3):142-146.
3.	Pritzker KP, Gay S, Jimenez SA, et al. Osteoarthritis cartilage histopathology:grading and staging. Osteoarthritis and Cartilage, 2006, 14(1):13-29.
4.	Gore M, Tai KS, Sadosky A, et al. Clinical comorbidities, treatment patterns, and direct medical costs of patients with osteoarthritis in usual care:a retrospective claims database analysis. J Med Econ, 2011, 14(4):497-507.
5.	Kuyinu EL, Narayanan G, Nair LS, et al. Animal models of osteoarthritis:classification, update, and measurement of outcomes. J Orthop Surg Res, 2016, 11:19.
6.	宁志刚, 杨柳, 王富友, 等. 保留钙化层结构的猪股骨滑车全厚软骨缺损模型建立. 中国修复重建外科杂志, 2012, 26(5):527-531.
7.	王斌. 骨性关节炎的动物模型研究进展. 中医临床研究, 2013, 5(10):120-122.
8.	翟吉良, 翁习生, 邱贵兴. 骨关节炎动物模型的建立及选择. 中国矫形外科杂志, 2007, 15(11):843-845.
9.	马玉峰, 祁印泽, 王庆甫, 等. 关节内注射药物建立骨性关节炎动物模型研究进展. 中国骨伤, 2015, 28(1):90-95.
10.	Lampropoulou-Adamidou K, Lelovas P, Karadimas EV, et al. Useful animal models for the research of osteoarthritis. Eur J Orthop Surg Traumatol, 2014, 24(3):263-271.
11.	Libicher M, Ivancic M, Hoffmann M, et al. Early changes in experimental osteoarthritis using the Pond-Nuki dog model:technical procedure and initial results of in vivo MR imaging. Eur Radiol, 2005, 15(2):390-394.
12.	Boileau C, Martel-Pelletier J, Abram F, et al. Magnetic resonance imaging can accurately assess the long-term progression of knee structural changes in experimental dog osteoarthritis. Ann Rheum Dis, 2008, 67(7):926-932.
13.	Shortkroff S, Barone L, Hsu HP, et al. Healing of chondral and osteochondral defects in a canine model:the role of cultured chondrocytes in regeneration of articular cartilage. Biomaterials, 1996, 17(2):147-154.
14.	Breinan HA, Minas T, Hsu HP, et al. Effect of cultured autologous chondrocytes on repair of chondral defects in a canine model. J Bone Joint Surg (Am), 1997, 79(10):1439-1451.
15.	项舟, 胡炜, 孔清泉, 等. 骨髓间充质干细胞种植Ⅰ型胶原支架材料修复关节软骨缺损的初步研究. 中国修复重建外科杂志, 2006, 20(2):148-154.
16.	D'Anjou MA, Moreau M, Troncy E, et al. Osteophytosis, subchondral bone sclerosis, joint effusion and soft tissue thickening in canine experimental stifle osteoarthritis:comparison between 1.5 T magnetic resonance imaging and computed radiography. Vet Surg, 2008, 37(2):166-177.
17.	Joseph GB, Hou SW, Nardo L, et al. MRI findings associated with development of incident knee pain over 48 months:data from the osteoarthritis initiative. Skeletal Radiol, 2016, 45(5):653-660.
18.	Podlipska J, Guermazi A, Lehenkari P, et al. Comparison of diagnostic performance of semi-quantitative knee ultrasound and knee radiography with MRI:oulu knee osteoarthritis study. Sci Rep, 2016, 6:22365.
19.	Roemer FW, Hunter DJ, Crema MD, et al. An illlustrative overview of semi-quantitative MRI scoring of knee osteoarthritis:lessons learned from longitudinal observational studies. Osteoarthritis Cartilage, 2016, 24(2):274-289.
20.	Teeple E, Jay GD, Elsaid KA, et al. Animal models of osteoarthritis:challenges of model selection and analysis. AAPS J, 2013, 15(2):438-446.

1. Tsuboguchi S, Matsui N, Taneda Y, et al. Arthroscopic findings of cartilage changes in knees with rheumatoid arthritis. Ryumachi, 1989, 29(2):110-117.
2. Frisbie DD, Cross MW, McIlwraith CW. A comparative study of articular cartilage thickness in the stifle of animal species used in human pre-clinical studies compared to articular cartilage thickness in the human knee. Vet Comp Orthop Traumatol, 2006, 19(3):142-146.
3. Pritzker KP, Gay S, Jimenez SA, et al. Osteoarthritis cartilage histopathology:grading and staging. Osteoarthritis and Cartilage, 2006, 14(1):13-29.
4. Gore M, Tai KS, Sadosky A, et al. Clinical comorbidities, treatment patterns, and direct medical costs of patients with osteoarthritis in usual care:a retrospective claims database analysis. J Med Econ, 2011, 14(4):497-507.
5. Kuyinu EL, Narayanan G, Nair LS, et al. Animal models of osteoarthritis:classification, update, and measurement of outcomes. J Orthop Surg Res, 2016, 11:19.
6. 宁志刚, 杨柳, 王富友, 等. 保留钙化层结构的猪股骨滑车全厚软骨缺损模型建立. 中国修复重建外科杂志, 2012, 26(5):527-531.
7. 王斌. 骨性关节炎的动物模型研究进展. 中医临床研究, 2013, 5(10):120-122.
8. 翟吉良, 翁习生, 邱贵兴. 骨关节炎动物模型的建立及选择. 中国矫形外科杂志, 2007, 15(11):843-845.
9. 马玉峰, 祁印泽, 王庆甫, 等. 关节内注射药物建立骨性关节炎动物模型研究进展. 中国骨伤, 2015, 28(1):90-95.
10. Lampropoulou-Adamidou K, Lelovas P, Karadimas EV, et al. Useful animal models for the research of osteoarthritis. Eur J Orthop Surg Traumatol, 2014, 24(3):263-271.
11. Libicher M, Ivancic M, Hoffmann M, et al. Early changes in experimental osteoarthritis using the Pond-Nuki dog model:technical procedure and initial results of in vivo MR imaging. Eur Radiol, 2005, 15(2):390-394.
12. Boileau C, Martel-Pelletier J, Abram F, et al. Magnetic resonance imaging can accurately assess the long-term progression of knee structural changes in experimental dog osteoarthritis. Ann Rheum Dis, 2008, 67(7):926-932.
13. Shortkroff S, Barone L, Hsu HP, et al. Healing of chondral and osteochondral defects in a canine model:the role of cultured chondrocytes in regeneration of articular cartilage. Biomaterials, 1996, 17(2):147-154.
14. Breinan HA, Minas T, Hsu HP, et al. Effect of cultured autologous chondrocytes on repair of chondral defects in a canine model. J Bone Joint Surg (Am), 1997, 79(10):1439-1451.
15. 项舟, 胡炜, 孔清泉, 等. 骨髓间充质干细胞种植Ⅰ型胶原支架材料修复关节软骨缺损的初步研究. 中国修复重建外科杂志, 2006, 20(2):148-154.
16. D'Anjou MA, Moreau M, Troncy E, et al. Osteophytosis, subchondral bone sclerosis, joint effusion and soft tissue thickening in canine experimental stifle osteoarthritis:comparison between 1.5 T magnetic resonance imaging and computed radiography. Vet Surg, 2008, 37(2):166-177.
17. Joseph GB, Hou SW, Nardo L, et al. MRI findings associated with development of incident knee pain over 48 months:data from the osteoarthritis initiative. Skeletal Radiol, 2016, 45(5):653-660.
18. Podlipska J, Guermazi A, Lehenkari P, et al. Comparison of diagnostic performance of semi-quantitative knee ultrasound and knee radiography with MRI:oulu knee osteoarthritis study. Sci Rep, 2016, 6:22365.
19. Roemer FW, Hunter DJ, Crema MD, et al. An illlustrative overview of semi-quantitative MRI scoring of knee osteoarthritis:lessons learned from longitudinal observational studies. Osteoarthritis Cartilage, 2016, 24(2):274-289.
20. Teeple E, Jay GD, Elsaid KA, et al. Animal models of osteoarthritis:challenges of model selection and analysis. AAPS J, 2013, 15(2):438-446.

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COMPARISON OF TWO ANIMAL MODELS WITH CARTILAGE DEFECT

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