PREPARATION OF PERSONALIZED BRAIN CAVITY SCAFFOLD WITH THREE-DIMENSIONAL PRINTING TECHNOLOGY BASED ON MAGNETIC RESONANCE IMAGING_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

FUFeng , ZHAOMingliang , LIXiaohong , CHENChong , WANGLina , SUNHongtao , TUYue ,  ZHANGSai

Tianjin Institute of Traumatic Brain Injury and Neurology, Brain Hospital of Affiliated Hospital of Logistics College of Chinese People's Armed Police Forces, Tianjin Key Laboratory of Neurotrauma Repair, Tianjin, 300162, P.R.China;

Corresponding author：

ZHANGSai, Email: zhangsai718@vip.126.com

Keywords：

Traumatic brain injury; Tissue engineering; Three-dimensional printing; Collagen-chitosan; Cavity scaffold; Rat

DOI：

10.7507/1002-1892.20160294

Video：

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Objective To explore a method of three-dimensional (3D) printing technology for preparation of personalized rat brain tissue cavity scaffolds so as to lay the foundation for the repair of traumatic brain injury (TBI) with tissue engineered customized cavity scaffolds. Methods Five male Sprague Dawley rats[weighing (300±10) g] were induced to TBI models by electric controlled cortical impactor. Mimics software was used to reconstruct the surface profile of the damaged cavity based on the MRI data, computer aided design to construct the internal structure. Then collagen-chitosan composite was prepared for 3D bioprinter of bionic brain cavity scaffold. Results MRI scans showed the changes of brain tissue injury in the injured side, and the position of the cavity was limited to the right side of the rat brain cortex. The 3D model of personalized cavity containing the internal structure was successfully constructed, and cavity scaffolds were prepared by 3D printing technology. The external contour of cavity scaffolds was similar to that of the injured zone in the rat TBI; the inner positive crossing structure arranged in order, and the pore connectivity was good. Conclusion Combined with 3D reconstruction based on MRI data, the appearance of cavity scaffolds by 3D printing technology is similar to that of injured cavity of rat brain tissue, and internal positive cross structure can simulate the topological structure of the extracellular matrix, and printing materials are collagen-chitosan complexes having good biocompatibility, so it will provide a new method for customized cavity scaffolds to repair brain tissue cavity after TBI.

Citation： FUFeng, ZHAOMingliang, LIXiaohong, CHENChong, WANGLina, SUNHongtao, TUYue, ZHANGSai. PREPARATION OF PERSONALIZED BRAIN CAVITY SCAFFOLD WITH THREE-DIMENSIONAL PRINTING TECHNOLOGY BASED ON MAGNETIC RESONANCE IMAGING. Chinese Journal of Reparative and Reconstructive Surgery, 2016, 30(11): 1425-1430. doi: 10.7507/1002-1892.20160294 Copy

1.	Stocchetti N, Zanier ER. Chronic impact of traumatic brain injury on outcome and quality of life: a narrative review. Crit Care, 2016, 20(1): 148.
2.	Bramlett HM, Dietrich WD. Long-term consequences of traumatic brain injury: current status of potential mechanisms of injury and neurological outcomes. J Neurotrauma, 2015, 32(23): 1834-1848.
3.	Johnson BN, Lancaster KZ, Zhen G, et al. 3D printed anatomical nerve regeneration pathways. Adv Funct Mater, 2015, 25(39): 6205-6217.
4.	Wong DY, Hollister SJ, Krebsbach PH, et al. Poly (epsilon-caprolactone) and poly (L-lactic-co-glycolic acid) degradable polymer sponges attenuate astrocyte response and lesion growth in acute traumatic brain injury. Tissue Eng, 2007, 13(10): 2515-2523.
5.	Yasuda H, Kuroda S, Shichinohe H, et al. Effect of biodegradable fibrin scaffold on survival, migration, and differentiation of transplanted bone marrow stromal cells after cortical injury in rats. J Neurosurg, 2010, 112(2): 336-344.
6.	Zhu W, O'Brien C, O'Brien JR, et al. 3D nano/microfabrication techniques and nanobiomaterials for neural tissue regeneration. Nanomedicine (Lond), 2014, 9(6): 859-875.
7.	Radenkovic D, Solouk A, Seifalian A. Personalized development of human organs using 3D printing technology. Med Hypotheses, 2016, 87: 30-33.
8.	Mitsouras D, Liacouras P, Imanzadeh A, et al. Medical 3D printing for the radiologist. Radiographics, 2015, 35(7): 1965-1988.
9.	Marro A, Bandukwala T, Mak W. Three-dimensional printing and medical imaging: areview of the methods and applications. Curr Probl Diagn Radiol, 2016, 45(1): 2-9.
10.	Rubenstein R, Chang B, Davies P, et al. A novel, ultrasensitive assay for tau: potential for assessing traumatic brain injury in tissues and biofluids. J Neurotrauma, 2015, 32(5):342-352.
11.	Johnstone VP, Wright DK, Wong K, et al. Experimental traumatic brain injury results in long-term recovery of functional responsiveness in sensory cortex but persisting structural changes and sensorimotor, cognitive, and emotional deficits. J Neurotrauma, 2015, 32(17): 1333-1346.
12.	樊继宏. Mimics建模到Marc有限元分析的系统工程和实例.广州:南方医科大学, 2010.
13.	Xu YY, Wu JM, Guan J, et al. Physiochemical and biological properties of modified collagen sponge from porcine skin.武汉理工大学学报(材料科学版):英文版, 2009, 24(4): 619-626.
14.	Bigler ED. Neuroinflammation and the dynamic lesion in traumatic brain injury. Brain, 2013, 136(Pt 1): 9-11.
15.	Leung GK, Wang YC, Wu W. Peptide nanofiber scaffold for brain tissue reconstruction. Methods Enzymol, 2012, 508: 177-190.
16.	Álvarez Z, Castaño O, Castells AA, et al. Neurogenesis and vascularization of the damaged brain using a lactate-releasing biomimetic scaffold. Biomaterials, 2014, 35(17): 4769-4781.
17.	Ju R, Wen Y, Gou R, et al. The experimental therapy on brain ischemia by improvement of local angiogenesis with tissue engineering in the mouse. Cell Transplant, 2014, 23 Suppl 1: S83-95.
18.	Klein GT, Lu Y, Wang MY. 3D printing and neurosurgery-ready for prime time? World Neurosurg, 2013, 80(3-4): 233-235.
19.	Wong DY, Krebsbach PH, Hollister SJ. Brain cortex regeneration affected by scaffold architectures. J Neurosurg, 2008, 109(4): 715-722.
20.	Maravelakis E, David K, Antoniadis A, et al. Reverse engineering techniques for cranioplasty: a case study. J Med Eng Technol, 2008, 32(2): 115-121.
21.	Naghdi P, Tiraihi T, Ganji F, et al. Survival, proliferation and differentiation enhancement of neural stem cells cultured in three-dimensional polyethylene glycol-RGD hydrogel with tenascin. J Tissue Eng Regen Med, 2016, 10(3):199-208.
22.	Chia HN, Wu BM. Recent advances in 3D printing of biomaterials. J Biol Eng, 2015, 9: 4.
23.	Ventola CL. Medical applications for 3D printing: current and projected uses. P T, 2014, 39(10): 704-711.
24.	Wüst S, Müller R, Hofmann S. 3D Bioprinting of complex channels-Effects of material, orientation, geometry, and cell embedding. J Biomed Mater Res A, 2015, 103(8): 2558-2570.
25.	Domingos M, Intranuovo F, Russo T, et al. The first systematic analysis of 3D rapid prototyped poly (epsilon-caprolactone) scaffolds manufactured through BioCell printing: the effect of pore size and geometry on compressive mechanical behaviour and in vitro hMSC viability. Biofabrication, 2013, 5(4): 045004.
26.	Mahmoud AA, Salama AH. Norfloxacin-loaded collagen/chitosan scaffolds for skin reconstruction: Preparation, evaluation and in-vivo wound healing assessment. Eur J Pharm Sci, 2016, 83: 155-165.
27.	Banglmaier RF, Sander EA, VandeVord PJ. Induction and quantification of collagen fiber alignment in a three-dimensional hydroxyapatite-collagen composite scaffold. Acta Biomater, 2015, 17:26-35.
28.	Zaminy A, Shokrgozar MA, Sadeghi Y, et al. Transplantation of schwann cells differentiated from adipose stem cells improves functional recovery in rat spinal cord injury. Arch Iran Med, 2013, 16(9): 533-541.
29.	Lee JE, Kim KE, Kwon IC, et al. Effects of the controlled-released TGF-beta 1 from chitosan microspheres on chondrocytes cultured in a collagen/chitosan/glycosaminoglycan scaffold. Biomaterials, 2004, 25(18): 4163-4173.
30.	陈淼彬, 李晓红, 吴森, 等.基于原子力显微镜的损伤星形胶质细胞弹性模量变化的研究.天津医药, 2016, 44(6): 665-668, 801-802.
31.	Sanz-Herrera JA, García-Aznar JM, Doblaré M. On scaffold designing for bone regeneration: a computational multiscale approach. Acta Biomater, 2009, 5(1): 219-229.
32.	Drury JL, Mooney DJ. Hydrogels for tissue engineering: scaffold design variables and applications. Biomaterials, 2003, 24(24): 4337-4351.

1. Stocchetti N, Zanier ER. Chronic impact of traumatic brain injury on outcome and quality of life: a narrative review. Crit Care, 2016, 20(1): 148.
2. Bramlett HM, Dietrich WD. Long-term consequences of traumatic brain injury: current status of potential mechanisms of injury and neurological outcomes. J Neurotrauma, 2015, 32(23): 1834-1848.
3. Johnson BN, Lancaster KZ, Zhen G, et al. 3D printed anatomical nerve regeneration pathways. Adv Funct Mater, 2015, 25(39): 6205-6217.
4. Wong DY, Hollister SJ, Krebsbach PH, et al. Poly (epsilon-caprolactone) and poly (L-lactic-co-glycolic acid) degradable polymer sponges attenuate astrocyte response and lesion growth in acute traumatic brain injury. Tissue Eng, 2007, 13(10): 2515-2523.
5. Yasuda H, Kuroda S, Shichinohe H, et al. Effect of biodegradable fibrin scaffold on survival, migration, and differentiation of transplanted bone marrow stromal cells after cortical injury in rats. J Neurosurg, 2010, 112(2): 336-344.
6. Zhu W, O'Brien C, O'Brien JR, et al. 3D nano/microfabrication techniques and nanobiomaterials for neural tissue regeneration. Nanomedicine (Lond), 2014, 9(6): 859-875.
7. Radenkovic D, Solouk A, Seifalian A. Personalized development of human organs using 3D printing technology. Med Hypotheses, 2016, 87: 30-33.
8. Mitsouras D, Liacouras P, Imanzadeh A, et al. Medical 3D printing for the radiologist. Radiographics, 2015, 35(7): 1965-1988.
9. Marro A, Bandukwala T, Mak W. Three-dimensional printing and medical imaging: areview of the methods and applications. Curr Probl Diagn Radiol, 2016, 45(1): 2-9.
10. Rubenstein R, Chang B, Davies P, et al. A novel, ultrasensitive assay for tau: potential for assessing traumatic brain injury in tissues and biofluids. J Neurotrauma, 2015, 32(5):342-352.
11. Johnstone VP, Wright DK, Wong K, et al. Experimental traumatic brain injury results in long-term recovery of functional responsiveness in sensory cortex but persisting structural changes and sensorimotor, cognitive, and emotional deficits. J Neurotrauma, 2015, 32(17): 1333-1346.
12. 樊继宏. Mimics建模到Marc有限元分析的系统工程和实例.广州:南方医科大学, 2010.
13. Xu YY, Wu JM, Guan J, et al. Physiochemical and biological properties of modified collagen sponge from porcine skin.武汉理工大学学报(材料科学版):英文版, 2009, 24(4): 619-626.
14. Bigler ED. Neuroinflammation and the dynamic lesion in traumatic brain injury. Brain, 2013, 136(Pt 1): 9-11.
15. Leung GK, Wang YC, Wu W. Peptide nanofiber scaffold for brain tissue reconstruction. Methods Enzymol, 2012, 508: 177-190.
16. Álvarez Z, Castaño O, Castells AA, et al. Neurogenesis and vascularization of the damaged brain using a lactate-releasing biomimetic scaffold. Biomaterials, 2014, 35(17): 4769-4781.
17. Ju R, Wen Y, Gou R, et al. The experimental therapy on brain ischemia by improvement of local angiogenesis with tissue engineering in the mouse. Cell Transplant, 2014, 23 Suppl 1: S83-95.
18. Klein GT, Lu Y, Wang MY. 3D printing and neurosurgery-ready for prime time? World Neurosurg, 2013, 80(3-4): 233-235.
19. Wong DY, Krebsbach PH, Hollister SJ. Brain cortex regeneration affected by scaffold architectures. J Neurosurg, 2008, 109(4): 715-722.
20. Maravelakis E, David K, Antoniadis A, et al. Reverse engineering techniques for cranioplasty: a case study. J Med Eng Technol, 2008, 32(2): 115-121.
21. Naghdi P, Tiraihi T, Ganji F, et al. Survival, proliferation and differentiation enhancement of neural stem cells cultured in three-dimensional polyethylene glycol-RGD hydrogel with tenascin. J Tissue Eng Regen Med, 2016, 10(3):199-208.
22. Chia HN, Wu BM. Recent advances in 3D printing of biomaterials. J Biol Eng, 2015, 9: 4.
23. Ventola CL. Medical applications for 3D printing: current and projected uses. P T, 2014, 39(10): 704-711.
24. Wüst S, Müller R, Hofmann S. 3D Bioprinting of complex channels-Effects of material, orientation, geometry, and cell embedding. J Biomed Mater Res A, 2015, 103(8): 2558-2570.
25. Domingos M, Intranuovo F, Russo T, et al. The first systematic analysis of 3D rapid prototyped poly (epsilon-caprolactone) scaffolds manufactured through BioCell printing: the effect of pore size and geometry on compressive mechanical behaviour and in vitro hMSC viability. Biofabrication, 2013, 5(4): 045004.
26. Mahmoud AA, Salama AH. Norfloxacin-loaded collagen/chitosan scaffolds for skin reconstruction: Preparation, evaluation and in-vivo wound healing assessment. Eur J Pharm Sci, 2016, 83: 155-165.
27. Banglmaier RF, Sander EA, VandeVord PJ. Induction and quantification of collagen fiber alignment in a three-dimensional hydroxyapatite-collagen composite scaffold. Acta Biomater, 2015, 17:26-35.
28. Zaminy A, Shokrgozar MA, Sadeghi Y, et al. Transplantation of schwann cells differentiated from adipose stem cells improves functional recovery in rat spinal cord injury. Arch Iran Med, 2013, 16(9): 533-541.
29. Lee JE, Kim KE, Kwon IC, et al. Effects of the controlled-released TGF-beta 1 from chitosan microspheres on chondrocytes cultured in a collagen/chitosan/glycosaminoglycan scaffold. Biomaterials, 2004, 25(18): 4163-4173.
30. 陈淼彬, 李晓红, 吴森, 等.基于原子力显微镜的损伤星形胶质细胞弹性模量变化的研究.天津医药, 2016, 44(6): 665-668, 801-802.
31. Sanz-Herrera JA, García-Aznar JM, Doblaré M. On scaffold designing for bone regeneration: a computational multiscale approach. Acta Biomater, 2009, 5(1): 219-229.
32. Drury JL, Mooney DJ. Hydrogels for tissue engineering: scaffold design variables and applications. Biomaterials, 2003, 24(24): 4337-4351.

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PREPARATION OF PERSONALIZED BRAIN CAVITY SCAFFOLD WITH THREE-DIMENSIONAL PRINTING TECHNOLOGY BASED ON MAGNETIC RESONANCE IMAGING

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