CURATIVE EFFECT OF HUMAN UMBILICAL CORD MESENCHYMAL STEM CELLS BY INTRA-ARTICULAR INJECTION FOR DEGENERATIVE KNEE OSTEOARTHRITIS_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

WANGYali ^1,2 , JINWenxiao ² , LIUHaiyan ² , CUIYuhua ² , MAOQingcong ² , FEIZhongying ² ,  XIANGChunsheng ¹

1. Medicine School of Zhejiang University, Hangzhou Zhejiang, 310058, P. R. China;
2. Biological Therapy Center of Zhejiang Armed Police Corps Jiaxing Hospital;

Corresponding author：

XIANGChunsheng, Email: cxiang@zju.edu.cn

Keywords：

Knee; Degenerative osteoarthritis; Human umbilical cord mesenchymal stem cells; Sodium hyaluronate; Intra-articular injection

DOI：

10.7507/1002-1892.20160305

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the safety and efficacy of human umbilical cord mesenchymal stem cells (MSCs) by intra-articular injection for degenerative knee osteoarthritis. Methods Between January 2015 and January 2016, 36 patients with moderate or severe degenerative knee osteoarthritis were randomly devided into 2 groups (n=18). Intra-articular injection of 2.5-3.0 mL human umbilical cord MSCs suspension containing (2-3)×10⁷ cells was performed once a month for 2 times as a course of treatment in the cell treatment group; sodium hyaluronate by intra-articular injection was used once a week for 5 times as a course of treatment in the control group. There was no significant difference in gender, age, body mass index, side, stage of osteoarthritis, course of disease, and preoperative Lysholm score of the knee joint, the Western Ontario and McMaster Universities osteoarthritis index (WOMAC), and SF-36 scale score between 2 groups (P > 0.05). The clinical efficacy was evaluated by SF-36 scale score, Lysholm score, and WOMAC score. Results All patients of 2 groups received a course of treatment. The patients were followed up for 6 months. After injection, the incidences of pain and swelling in the cell treatment group were significantly higher than those in the control group (χ²=16.200, P=0.000; χ²=11.688, P=0.000), but no significant difference was found in the incidence of effusion (χ²=2.118, P=0.146). In the cell treatment group, Lysholm score at 1-6 months after treatment, WOMAC score and SF-36 scale score at 2-6 months after treatment were significantly better when compared with scores before treatment (P < 0.05), and no recurrence of knee pain was observed during follow-up. In the control group, there was no significant difference in Lysholm score and SF-36 scale score between pre-and post-treatment (P > 0.05); there were significant differences in WOMAC score between pre-treatment and at 1, 2, 3 months after treatment (P < 0.05); at 3 months after treatment, 11 patients had joint pain symptoms again. No significant difference was found in the knee joint function score and SF-36 scale score at 1 and 2 months after treatment between 2 groups (P > 0.05), but the scores of the cell treatment group were significantly better than those of the control group at 3 and 6 months (P < 0.05). Conclusion It can significantly improve the joint function and quality of life to use intra-articular injection of human umbilical cord MSCs for treating degenerative knee osteoarthritis. It takes effect after 1 month and the treatment effect can be sustained for 6 months.

Citation： WANGYali, JINWenxiao, LIUHaiyan, CUIYuhua, MAOQingcong, FEIZhongying, XIANGChunsheng. CURATIVE EFFECT OF HUMAN UMBILICAL CORD MESENCHYMAL STEM CELLS BY INTRA-ARTICULAR INJECTION FOR DEGENERATIVE KNEE OSTEOARTHRITIS. Chinese Journal of Reparative and Reconstructive Surgery, 2016, 30(12): 1472-1477. doi: 10.7507/1002-1892.20160305 Copy

1.	吴成爱, 赵丹慧, 张砚卓, 等.骨科退行性疾病临床与生物样本整合平台的建立.中华骨科杂志, 2016, 36(5):271-276.
2.	路莹, 车明学, 张震宇, 等.玻璃酸钠联合曲安奈德治疗中老年膝关节炎的疗效.中国老年学杂志, 2014, 34(21):6208-6209.
3.	王黎明, 王立华, 李铭, 等.脐带间充质干细胞移植对类风湿性关节炎患者行管炎性因子影响的探讨.中国免疫学杂志, 2014, 30(8):1059-1063.
4.	王亚莉, 齐江彤, 崔玉华, 等.人脐带间充质干细胞移植治疗强直性脊柱炎.免疫学杂志, 2015, 31(1):90-92.
5.	Uth K, Trifonov D. Stem cell application for osteoarthritis in the knee joint:A minireview. World J Stem Cells, 2014, 6(5):629-636.
6.	Orth P, Rey-Rico A, Venkatesan JK, et al. Current perspectives in stem cell research for knee cartilage repair. Stem Cells Cloning, 2014, 7:1-17.
7.	王向辉, 丁琼浩.膝骨关节炎的骨髓间充质干细胞疗法.现代中西医结合杂志, 2015, 24(4):451-453.
8.	于洋, 李鸿斌.透明质酸诱导骨髓间充质干细胞治疗骨性关节炎软骨分化研究进展.内蒙古医学杂志, 2013, 45(4):438-441.
9.	Freitag J, Bates D, Boyd R, et al. Mesenchymal stem cell therapy in the treatment of osteoarthritis:reparative pathways, safety and efficacy-a review. BMC Musculoskelet Disord, 2016, 17:230.
10.	Wolfstadt JI, Cole BJ, Ogilvie-Harris DJ, et al. Current concepts:the role of mesenchymal stem cells in the management of knee osteoarthritis. Sports Health, 2015, 7(1):38-44.
11.	Chen G, Wang Y, Xu Z, et al. Neural stem cell-like cells derived from autologous bone mesenchymal stem cells for the treatment of patients with cerebral palsy. J Transl Med, 2013, 11:21.
12.	王亚莉, 陈国军, 方凤, 等.脑瘫患者骨髓间充质干细胞体外培养特性分析.山东医药, 2012, 52(23):8-10.
13.	刘时璋, 凌鸣, 易智, 等.间充质干细胞在兔骨关节炎关节腔内移植的研究.陕西医学杂志, 2013, 42(11):1450-1452.
14.	罗玉明, 郑维篷, 魏合伟.骨关节炎与细胞因子TNF-α、IL-6关系的研究进展.现代诊断与治疗, 2013, 24(2):326-327.
15.	潘建科, 郭达, 刘军, 等.白细胞介素-10与骨关节炎相关性的研究进展.广东医学, 2016, 37(1):146-148.
16.	康丽莉, 于立耘, 倪军, 等.脐带间充质干细胞移植对胶原诱导型关节炎大鼠炎性趋化因子表达的影响.血栓与止血学, 2012, 18(6):247-250.
17.	林传明, 顾健, 张育, 等.脐带间充质干细胞移植对胶原诱导型关节炎大鼠免疫相关易栓状态的干预作用.中华血液学杂志, 2012, 33(3):215-219.
18.	金瑛, 李豫皖, 张承昊, 等.体外诱导人羊膜间充质干细胞向韧带细胞分化的实验研究.中国修复重建外科杂志, 2016, 30(2):237-244.

1. 吴成爱, 赵丹慧, 张砚卓, 等.骨科退行性疾病临床与生物样本整合平台的建立.中华骨科杂志, 2016, 36(5):271-276.
2. 路莹, 车明学, 张震宇, 等.玻璃酸钠联合曲安奈德治疗中老年膝关节炎的疗效.中国老年学杂志, 2014, 34(21):6208-6209.
3. 王黎明, 王立华, 李铭, 等.脐带间充质干细胞移植对类风湿性关节炎患者行管炎性因子影响的探讨.中国免疫学杂志, 2014, 30(8):1059-1063.
4. 王亚莉, 齐江彤, 崔玉华, 等.人脐带间充质干细胞移植治疗强直性脊柱炎.免疫学杂志, 2015, 31(1):90-92.
5. Uth K, Trifonov D. Stem cell application for osteoarthritis in the knee joint:A minireview. World J Stem Cells, 2014, 6(5):629-636.
6. Orth P, Rey-Rico A, Venkatesan JK, et al. Current perspectives in stem cell research for knee cartilage repair. Stem Cells Cloning, 2014, 7:1-17.
7. 王向辉, 丁琼浩.膝骨关节炎的骨髓间充质干细胞疗法.现代中西医结合杂志, 2015, 24(4):451-453.
8. 于洋, 李鸿斌.透明质酸诱导骨髓间充质干细胞治疗骨性关节炎软骨分化研究进展.内蒙古医学杂志, 2013, 45(4):438-441.
9. Freitag J, Bates D, Boyd R, et al. Mesenchymal stem cell therapy in the treatment of osteoarthritis:reparative pathways, safety and efficacy-a review. BMC Musculoskelet Disord, 2016, 17:230.
10. Wolfstadt JI, Cole BJ, Ogilvie-Harris DJ, et al. Current concepts:the role of mesenchymal stem cells in the management of knee osteoarthritis. Sports Health, 2015, 7(1):38-44.
11. Chen G, Wang Y, Xu Z, et al. Neural stem cell-like cells derived from autologous bone mesenchymal stem cells for the treatment of patients with cerebral palsy. J Transl Med, 2013, 11:21.
12. 王亚莉, 陈国军, 方凤, 等.脑瘫患者骨髓间充质干细胞体外培养特性分析.山东医药, 2012, 52(23):8-10.
13. 刘时璋, 凌鸣, 易智, 等.间充质干细胞在兔骨关节炎关节腔内移植的研究.陕西医学杂志, 2013, 42(11):1450-1452.
14. 罗玉明, 郑维篷, 魏合伟.骨关节炎与细胞因子TNF-α、IL-6关系的研究进展.现代诊断与治疗, 2013, 24(2):326-327.
15. 潘建科, 郭达, 刘军, 等.白细胞介素-10与骨关节炎相关性的研究进展.广东医学, 2016, 37(1):146-148.
16. 康丽莉, 于立耘, 倪军, 等.脐带间充质干细胞移植对胶原诱导型关节炎大鼠炎性趋化因子表达的影响.血栓与止血学, 2012, 18(6):247-250.
17. 林传明, 顾健, 张育, 等.脐带间充质干细胞移植对胶原诱导型关节炎大鼠免疫相关易栓状态的干预作用.中华血液学杂志, 2012, 33(3):215-219.
18. 金瑛, 李豫皖, 张承昊, 等.体外诱导人羊膜间充质干细胞向韧带细胞分化的实验研究.中国修复重建外科杂志, 2016, 30(2):237-244.

Chinese Journal of Reparative and Reconstructive Surgery

CURATIVE EFFECT OF HUMAN UMBILICAL CORD MESENCHYMAL STEM CELLS BY INTRA-ARTICULAR INJECTION FOR DEGENERATIVE KNEE OSTEOARTHRITIS

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content