Effect of a new front opening liposuction cannula on survival of transplanted fat tissue_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

TAN Xi ¹ , LEI Xiaoxuan ² , ZHENG Zhifang ² , CHENG Biao ² , ZOU Yanlong ¹ , WANG Hongxia ¹ ,  WANG Zhenxiang ¹

1. Department of Plastic and Reconstructive Surgery, the First Affiliated Hospital, Army Medical University, Chongqing, 400038, P.R.China;
2. Department of Plastic Surgery, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou Guangdong, 510010, P.R.China;

Corresponding author：

WANG Zhenxiang, Email: 413040369@qq.com

Keywords：

Fat transplantation; new front opening liposuction cannula; adipocyte viability

DOI：

10.7507/1002-1892.201711026

Video：

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ObjectiveTo investigate the effect of a new front opening liposuction cannula on the survival of transplanted fat tissue.MethodsTwo groups of fats were obtained from the left and right sides of a female patient during the abdomen liposuction surgery, respectively. And the fats in experimental group and control group were harvested by the new front opening liposuction cannula and the side hole liposuction cannula, respectively. The differences of adipocyte activity in vitro between 2 groups were compared by observation under the electron scanning microscopy and the glucose transportation test. Then, the fats in 2 groups (n=20) were injected subcutaneously into the back of 20 nude mice (400 mg fats per injection zone). The differences of the injection area reactions, remaining weight, histological characteristics, and microvessel density (MVD) between 2 groups were compared after 4 weeks and 12 weeks.ResultsCompared with the control group, in vitro, the adipocytes were more plump and the vascular structures were more abundant. The glucose transportation quantities were (3.049±0.266) mmol/L and (2.668±0.250) mmol/L in experimental and control groups, showing significant difference between groups (t=2.956, P=0.010). There was only one fat liquefaction occurred in the injection zone of the control group after 4 weeks. The experimental group had more clear adipocytes, more vessels, and less inflammation and necrosis than the control group. The remaining weight and MVD were higher in the experimental group than in the control group after 4 and 12 weeks, showing significant differences (P<0.05).ConclusionThe new front opening liposuction cannula can reduce the damage of adipocytes and improve the survival of transplanted fat tissue.

Citation： TAN Xi, LEI Xiaoxuan, ZHENG Zhifang, CHENG Biao, ZOU Yanlong, WANG Hongxia, WANG Zhenxiang. Effect of a new front opening liposuction cannula on survival of transplanted fat tissue. Chinese Journal of Reparative and Reconstructive Surgery, 2018, 32(3): 363-368. doi: 10.7507/1002-1892.201711026 Copy

1.	Bellini E, Grieco MP, Raposio E. The science behind autologous fat grafting. Ann Med Surg (Lond), 2017, 24: 65-73.
2.	Sinno S, Wilson S, Brownstone N, et al. Current thoughts on fat grafting: Using the evidence to determine fact or fiction. Plast Reconstr Surg, 2016, 137(3): 818-824.
3.	Negenborn VL, Groen JW, Smit JM, et al. The use of autologous fat grafting for treatment of scar tissue and scar-related conditions: A systematic review. Plast Reconstr Surg, 2016, 137(1): 31e-43e.
4.	Moustaki M, Papadopoulos O, Verikokos C, et al. Application of adipose-derived stromal cells in fat grafting: Basic science and literature review. Exp Ther Med, 2017, 14(3): 2415-2423.
5.	Laloze J, Varin A, Gilhodes J, et al. Cell-assisted lipotransfer: friend or foe in fat grafting? Systematic review and meta-analysis. J Tissue Eng Regen Med, 2017.[Epub ahead of print].
6.	Kato H, Mineda K, Eto H, et al. Degeneration, regeneration, and cicatrization after fat grafting: dynamic total tissue remodeling during the first 3 months. Plast Reconstr Surg, 2014, 133(3): 303e-313e.
7.	Toyserkani NM, Quaade ML, Sørensen JA. Cell-assisted lipotransfer: A systematic review of its efficacy. Aesthetic Plast Surg, 2016, 40(2): 309-318.
8.	Gause TM 2nd, Kling RE, Sivak WN, et al. Particle size in fat graft retention: A review on the impact of harvesting technique in lipofilling surgical outcomes. Adipocyte, 2014, 3(4): 273-279.
9.	Eto H, Kato H, Suga H, et al. The fate of adipocytes after nonvascularized fat grafting: evidence of early death and replacement of adipocytes. Plast Reconstr Surg, 2012, 129(5): 1081-1092.
10.	Peltoniemi HH, Salmi A, Miettinen S, et al. Stem cell enrichment does not warrant a higher graft survival in lipofilling of the breast: a prospective comparative study. J Plast Reconstr Aesthet Surg, 2013, 66(11): 1494-1503.
11.	Del Vecchio D, Rohrich RJ. A classification of clinical fat grafting: different problems, different solutions. Plast Reconstr Surg, 2012, 130(3): 511-522.
12.	Pu LL, Coleman SR, Cui X, et al. Autologous fat grafts harvested and refined by the Coleman technique: a comparative study. Plast Reconstr Surg, 2008, 122(3): 932-937.
13.	Chen YW, Wang JR, Liao X, et al. Effect of suction pressures on cell yield and functionality of the adipose-derived stromal vascular fraction. J Plast Reconstr Aesthet Surg, 2017, 70(2): 257-266.
14.	杨加富, 宋瑜, 侯帅, 等. 医用注射器抽吸压力的测定. 中国美容整形外科杂志, 2010, 21(12): 734-736.
15.	赵玉坤, 于冬梅, 罗赛, 等. 体外纯化方法对脂肪颗粒细胞活性的影响. 中国美容整形外科杂志, 2016, 27(6): 378-380.
16.	雷华, 李青峰. 葡萄糖转移方法检测脂肪颗粒活性的研究. 中华整形外科杂志, 2005, 21(5): 375-378.Li L, Pan S, Ni B, et al. Improvement in autologous human fat transplant survival with SVF plus VEGF-PLA nano-sustained release microspheres. Cell Biol Int, 2014, 38(8): 962-970.
17.	Li K, Li F, Li J, et al. Increased survival of human free fat grafts with varying densities of human adipose-derived stem cells and platelet-rich plasma. J Tissue Eng Regen Med, 2017, 11(1): 209-219.

1. Bellini E, Grieco MP, Raposio E. The science behind autologous fat grafting. Ann Med Surg (Lond), 2017, 24: 65-73.
2. Sinno S, Wilson S, Brownstone N, et al. Current thoughts on fat grafting: Using the evidence to determine fact or fiction. Plast Reconstr Surg, 2016, 137(3): 818-824.
3. Negenborn VL, Groen JW, Smit JM, et al. The use of autologous fat grafting for treatment of scar tissue and scar-related conditions: A systematic review. Plast Reconstr Surg, 2016, 137(1): 31e-43e.
4. Moustaki M, Papadopoulos O, Verikokos C, et al. Application of adipose-derived stromal cells in fat grafting: Basic science and literature review. Exp Ther Med, 2017, 14(3): 2415-2423.
5. Laloze J, Varin A, Gilhodes J, et al. Cell-assisted lipotransfer: friend or foe in fat grafting? Systematic review and meta-analysis. J Tissue Eng Regen Med, 2017.[Epub ahead of print].
6. Kato H, Mineda K, Eto H, et al. Degeneration, regeneration, and cicatrization after fat grafting: dynamic total tissue remodeling during the first 3 months. Plast Reconstr Surg, 2014, 133(3): 303e-313e.
7. Toyserkani NM, Quaade ML, Sørensen JA. Cell-assisted lipotransfer: A systematic review of its efficacy. Aesthetic Plast Surg, 2016, 40(2): 309-318.
8. Gause TM 2nd, Kling RE, Sivak WN, et al. Particle size in fat graft retention: A review on the impact of harvesting technique in lipofilling surgical outcomes. Adipocyte, 2014, 3(4): 273-279.
9. Eto H, Kato H, Suga H, et al. The fate of adipocytes after nonvascularized fat grafting: evidence of early death and replacement of adipocytes. Plast Reconstr Surg, 2012, 129(5): 1081-1092.
10. Peltoniemi HH, Salmi A, Miettinen S, et al. Stem cell enrichment does not warrant a higher graft survival in lipofilling of the breast: a prospective comparative study. J Plast Reconstr Aesthet Surg, 2013, 66(11): 1494-1503.
11. Del Vecchio D, Rohrich RJ. A classification of clinical fat grafting: different problems, different solutions. Plast Reconstr Surg, 2012, 130(3): 511-522.
12. Pu LL, Coleman SR, Cui X, et al. Autologous fat grafts harvested and refined by the Coleman technique: a comparative study. Plast Reconstr Surg, 2008, 122(3): 932-937.
13. Chen YW, Wang JR, Liao X, et al. Effect of suction pressures on cell yield and functionality of the adipose-derived stromal vascular fraction. J Plast Reconstr Aesthet Surg, 2017, 70(2): 257-266.
14. 杨加富, 宋瑜, 侯帅, 等. 医用注射器抽吸压力的测定. 中国美容整形外科杂志, 2010, 21(12): 734-736.
15. 赵玉坤, 于冬梅, 罗赛, 等. 体外纯化方法对脂肪颗粒细胞活性的影响. 中国美容整形外科杂志, 2016, 27(6): 378-380.
16. 雷华, 李青峰. 葡萄糖转移方法检测脂肪颗粒活性的研究. 中华整形外科杂志, 2005, 21(5): 375-378.Li L, Pan S, Ni B, et al. Improvement in autologous human fat transplant survival with SVF plus VEGF-PLA nano-sustained release microspheres. Cell Biol Int, 2014, 38(8): 962-970.
17. Li K, Li F, Li J, et al. Increased survival of human free fat grafts with varying densities of human adipose-derived stem cells and platelet-rich plasma. J Tissue Eng Regen Med, 2017, 11(1): 209-219.

Chinese Journal of Reparative and Reconstructive Surgery

Effect of a new front opening liposuction cannula on survival of transplanted fat tissue

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