Progress in the application of silk fibroin in tissue engineered drug delivery system_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

LI Shengtang ^1,2 , SHI Xuewen ² , XU Bo ^1,2 , ZHEN Ping ² ,  LI Songkai ²

1. The Second Clinical Medical College of Lanzhou University, Lanzhou Gansu, 730000, P.R.China;
2. Department of Orthopaedics, the 940 Hospital of PLA Joint Logistics Support Force, Lanzhou Gansu, 730050, P.R.China;

Corresponding author：

LI Songkai, Email: lisongkai@gmail.com

Keywords：

Silk fibroin; sustained drug release; tissue engineering

DOI：

10.7507/1002-1892.202103066

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective The properties and characteristics of different types of silk fibroin (SF) drug-loaded sustained-release carriers and their effects on the drug release behavior were reviewed, and the existing problems and development prospects of SF drug-loaded sustained-release carriers in tissue engineering drug delivery system were discussed.Methods The literatures about drug-loaded SF sustained-release carriers in recent years were extensively consulted, and the types of sustained-release carriers, characteristics of drug release, range of applications, advantages and disadvantages, and solutions were summarized and analyzed.Results At present, the SF drug-loaded sustained-release carriers are mainly divided into SF microparticles, SF scaffolds, SF membranes, SF hydrogels, SF nanofibers, SF sponges, and so on. These types of SF drug-loaded sustained-release carriers have their own advantages and problems, of which the most prominent problem is the burst release of drugs at the initial stage. While, the initial burst release of drugs can be effectively solved by improving the preparation process and adjusting the material ratio. Different types of drug-loaded sustained-release carriers can be prepared by combining different materials to achieve different application scopes and drug release behaviors under different conditions.Conclusion SF is a good drug-loaded carrier for tissue engineering, the burst release of drugs at the initial stage can be solved by improving the preparation process and changing the material structure; through the combination of the advantages of various types of SF drug-loaded sustained-release carriers, it is expected to prepare SF drug-loaded sustained-release carriers that meet different clinical needs.

Citation： LI Shengtang, SHI Xuewen, XU Bo, ZHEN Ping, LI Songkai. Progress in the application of silk fibroin in tissue engineered drug delivery system. Chinese Journal of Reparative and Reconstructive Surgery, 2021, 35(9): 1192-1199. doi: 10.7507/1002-1892.202103066 Copy

1.	Huang W, Ling S, Li C, et al. Silkworm silk-based materials and devices generated using bio-nanotechnology. Chem Soc Rev, 2018, 47(17): 6486-6504.
2.	Tomeh MA, Hadianamrei R, Zhao X. Silk fibroin as a functional biomaterial for drug and gene delivery. Pharmaceutics, 2019, 11(10): 494. doi: 10.3390/pharmaceutics11100494.
3.	Tran SH, Wilson CG, Seib FP. A review of the emerging role of silk for the treatment of the eye. Pharm Res, 2018, 35(12): 248. doi: 10.1007/s11095-018-2534-y.
4.	Wani SUD, Gautam SP, Qadrie ZL, et al. Silk fibroin as a natural polymeric based bio-material for tissue engineering and drug delivery systems-A review. Int J Biol Macromol, 2020, 163: 2145-2161.
5.	Farokhi M, Mottaghitalab F, Reis RL, et al. Functionalized silk fibroin nanofibers as drug carriers: Advantages and challenges. J Control Release, 2020, 321: 324-347.
6.	Zheng H, Zuo B. Functional silk fibroin hydrogels: preparation, properties and applications. J Mater Chem B, 2021, 9(5): 1238-1258.
7.	Quartinello F, Tallian C, Auer J, et al. Smart textiles in wound care: functionalization of cotton/PET blends with antimicrobial nanocapsules. J Mater Chem B, 2019, 7(42): 6592-6603.
8.	Tallian C, Herrero-Rollett A, Stadler K, et al. Structural insights into pH-responsive drug release of self-assembling human serum albumin-silk fibroin nanocapsules. Eur J Pharm Biopharm, 2018, 133: 176-187.
9.	Yonesi M, Garcia-Nieto M, Guinea GV, et al. Silk fibroin: An ancient material for repairing the injured nervous system. Pharmaceutics, 2021, 13(3): 429. doi: 10.3390/pharmaceutics13030429.
10.	Galam N, Tulay P, Adali T. In vitro MCF-7 cells apoptosis analysis of carboplatin loaded silk fibroin particles. Molecules, 2020, 25(5): 1110. doi: 10.3390/molecules25051110.
11.	Shen X, Zhang Y, Gu Y, et al. Sequential and sustained release of SDF-1 and BMP-2 from silk fibroin-nanohydroxyapatite scaffold for the enhancement of bone regeneration. Biomaterials, 2016, 106: 205-216.
12.	Sharma S, Bano S, Ghosh AS, et al. Silk fibroin nanoparticles support in vitro sustained antibiotic release and osteogenesis on titanium surface. Nanomedicine, 2016, 12(5): 1193-1204.
13.	Zhao X, Chen Z, Liu Y, et al. Silk fibroin microparticles with hollow mesoporous silica nanocarriers encapsulation for abdominal wall repair. Adv Healthc Mater, 2018, 7(21): e1801005. doi: 10.1002/adhm.201801005.
14.	Luo J, Zhang H, Zhu J, et al. 3-D mineralized silk fibroin/polycaprolactone composite scaffold modified with polyglutamate conjugated with BMP-2 peptide for bone tissue engineering. Colloids Surf B Biointerfaces, 2018, 163: 369-378.
15.	Zhang L, Xu L, Li G, et al. Fabrication of high-strength mecobalamin loaded aligned silk fibroin scaffolds for guiding neuronal orientation. Colloids Surf B Biointerfaces, 2019, 173: 689-697.
16.	Xu R, Zhang Z, Toftdal MS, et al. Synchronous delivery of hydroxyapatite and connective tissue growth factor derived osteoinductive peptide enhanced osteogenesis. J Control Release, 2019, 301: 129-139.
17.	Ou L, Lan Y, Feng Z, et al. Functionalization of SF/HAP scaffold with GO-PEI-miRNA inhibitor complexes to enhance bone regeneration through activating transcription factor 4. Theranostics, 2019, 9(15): 4525-4541.
18.	Yang Q, Teng BH, Wang LN, et al. Silk fibroin/cartilage extracellular matrix scaffolds with sequential delivery of TGF-β₃ for chondrogenic differentiation of adipose-derived stem cells. Int J Nanomedicine, 2017, 12: 6721-6733.
19.	Wang Q, Cao L, Liu Y, et al. Evaluation of synergistic osteogenesis between icariin and BMP2 through a micro/meso hierarchical porous delivery system. Int J Nanomedicine, 2017, 12: 7721-7735.
20.	Xin X, Wu J, Zheng A, et al. Delivery vehicle of muscle-derived irisin based on silk/calcium silicate/sodium alginate composite scaffold for bone regeneration. Int J Nanomedicine, 2019, 14: 1451-1467.
21.	Shera SS, Sahu S, Banik RM. Preparation of drug eluting natural composite scaffold using response surface methodology and artificial neural network approach. Tissue Eng Regen Med, 2018, 15(2): 131-143.
22.	黄文良, 叶鹏, 莫刚, 等. 制备缓释骨形态发生蛋白2的丝素蛋白/壳聚糖/纳米羟基磷灰石生物支架. 中国组织工程研究, 2017, 21(22): 3488-3493.
23.	Ramadass SK, Nazir LS, Thangam R, et al. Type Ⅰ collagen peptides and nitric oxide releasing electrospun silk fibroin scaffold: A multifunctional approach for the treatment of ischemic chronic wounds. Colloids Surf B Biointerfaces, 2019, 175: 636-643.
24.	Li M, Ai M, Yang Y, et al. Silk-coated dexamethasone non-spherical microcrystals for local drug delivery to inner ear. Eur J Pharm Sci, 2020, 150: 105336. doi: 10.1016/j.ejps.2020.105336.
25.	Li G, Che MT, Zhang K, et al. Graft of the NT-3 persistent delivery gelatin sponge scaffold promotes axon regeneration, attenuates inflammation, and induces cell migration in rat and canine with spinal cord injury. Biomaterials, 2016, 83: 233-248.
26.	Li G, Che MT, Zeng X, et al. Neurotrophin-3 released from implant of tissue-engineered fibroin scaffolds inhibits inflammation, enhances nerve fiber regeneration, and improves motor function in canine spinal cord injury. J Biomed Mater Res A, 2018, 106(8): 2158-2170.
27.	Bhattacharjee P, Naskar D, Maiti TK, et al. Non-mulberry silk fibroin grafted poly (Є-caprolactone)/nano hydroxyapatite nanofibrous scaffold for dual growth factor delivery to promote bone regeneration. J Colloid Interface Sci, 2016, 472: 16-33.
28.	Zhou WH, Zhang T, Yan JL, et al. In vitro and in vivo evaluation of structurally-controlled silk fibroin coatings for orthopedic infection and in-situ osteogenesis. Acta Biomater, 2020, 116: 223-245.
29.	Xiao W, Zhang J, Qu X, et al. Fabrication of protease ⅩⅣ-loaded microspheres for cell spreading in silk fibroin hydrogels. J Mater Sci Mater Med, 2020, 31(12): 128. doi: 10.1007/s10856-020-06466-7.
30.	Shao J, Ding Z, Li L, et al. Improved accumulation of TGF-β by photopolymerized chitosan/silk protein bio-hydrogel matrix to improve differentiations of mesenchymal stem cells in articular cartilage tissue regeneration. J Photochem Photobiol B, 2020, 203: 111744. doi: 10.1016/j.jphotobiol.2019.111744.
31.	Ziadlou R, Rotman S, Teuschl A, et al. Optimization of hyaluronic acid-tyramine/silk-fibroin composite hydrogels for cartilage tissue engineering and delivery of anti-inflammatory and anabolic drugs. Mater Sci Eng C Mater Biol Appl, 2021, 120: 111701. doi: 10.1016/j.msec.2020.111701.
32.	He S, Shi D, Han Z, et al. Heparinized silk fibroin hydrogels loading FGF1 promote the wound healing in rats with full-thickness skin excision. Biomed Eng Online, 2019, 18(1): 97. doi: 10.1186/s12938-019-0716-4.
33.	Oliveira IM, Gonçalves C, Shin ME, et al. Anti-inflammatory properties of injectable betamethasone-loaded tyramine-modified gellan gum/silk fibroin hydrogels. Biomolecules, 2020, 10(10): 1456. doi: 10.3390/biom10101456.
34.	Laomeephol C, Ferreira H, Kanokpanont S, et al. Dual-functional liposomes for curcumin delivery and accelerating silk fibroin hydrogel formation. Int J Pharm, 2020, 589: 119844. doi: 10.1016/j.ijpharm.2020.119844.
35.	Youn J, Choi JH, Lee S, et al. Pluronic F-127/silk fibroin for enhanced mechanical property and sustained release drug for tissue engineering biomaterial. Materials (Basel), 2021, 14(5): 1287. doi: 10.3390/ma14051287.
36.	Yan S, Wang Q, Tariq Z, et al. Facile preparation of bioactive silk fibroin/hyaluronic acid hydrogels. Int J Biol Macromol, 2018, 118(Pt A): 775-782.
37.	Wu J, Zheng K, Huang X, et al. Thermally triggered injectable chitosan/silk fibroin/bioactive glass nanoparticle hydrogels for in-situ bone formation in rat calvarial bone defects. Acta Biomater, 2019, 91: 60-71.
38.	Xu X, Wang X, Qin C, et al. Silk fibroin/poly-(L-lactide-co-caprolactone) nanofiber scaffolds loaded with Huangbai Liniment to accelerate diabetic wound healing. Colloids Surf B Biointerfaces, 2021, 199: 111557. doi: 10.1016/j.colsurfb.2021.111557.
39.	Hadisi Z, Farokhi M, Bakhsheshi-Rad HR, et al. Hyaluronic acid (HA)-based silk fibroin/zinc oxide core-shell electrospun dressing for burn wound management. Macromol Biosci, 2020, 20(4): e1900328. doi: 10.1002/mabi.201900328.
40.	Wang Z, Song X, Cui Y, et al. Silk fibroin H-fibroin/poly(ε-caprolactone) core-shell nanofibers with enhanced mechanical property and long-term drug release. J Colloid Interface Sci, 2021, 593: 142-151.
41.	Khan AUR, Nadeem M, Bhutto MA, et al. Physico-chemical and biological evaluation of PLCL/SF nanofibers loaded with oregano essential oil. Pharmaceutics, 2019, 11(8): 386. doi: 10.3390/pharmaceutics11080386.
42.	Dadras Chomachayi M, Solouk A, Akbari S, et al. Electrospun nanofibers comprising of silk fibroin/gelatin for drug delivery applications: Thyme essential oil and doxycycline monohydrate release study. J Biomed Mater Res A, 2018, 106(4): 1092-1103.
43.	Chouhan D, Chakraborty B, Nandi SK, et al. Role of non-mulberry silk fibroin in deposition and regulation of extracellular matrix towards accelerated wound healing. Acta Biomater, 2017, 48: 157-174.
44.	Song J, Klymov A, Shao J, et al. Electrospun nanofibrous silk fibroin membranes containing gelatin nanospheres for controlled delivery of biomolecules. Adv Healthc Mater, 2017, 6(14). doi: 10.1002/adhm.201700014.
45.	Kalani MM, Nourmohammadi J, Negahdari B, et al. Electrospun core-sheath poly (vinyl alcohol)/silk fibroin nanofibers with Rosuvastatin release functionality for enhancing osteogenesis of human adipose-derived stem cells. Mater Sci Eng C Mater Biol Appl, 2019, 99: 129-139.
46.	Xu R, Zhao H, Muhammad H, et al. Dual-delivery of FGF-2/CTGF from silk fibroin/PLCL-PEO coaxial fibers enhances MSC proliferation and fibrogenesis. Sci Rep, 2017, 7(1): 8509. doi: 10.1038/s41598-017-08226-0.
47.	Silva SS, Oliveira NM, Oliveira MB, et al. Fabrication and characterization of Eri silk fibers-based sponges for biomedical application. Acta Biomater, 2016, 32: 178-189.
48.	Wang Q, Qian Z, Liu B, et al. In vitro and in vivo evaluation of new PRP antibacterial moisturizing dressings for infectious wound repair. J Biomater Sci Polym Ed, 2019, 30(6): 462-485.
49.	Naskar D, Ghosh AK, Mandal M, et al. Dual growth factor loaded nonmulberry silk fibroin/carbon nanofiber composite 3D scaffolds for in vitro and in vivo bone regeneration. Biomaterials, 2017, 136: 67-85.
50.	Liu M, Zhang Y, Liu K, et al. Biomimicking antibacterial opto-electro sensing sutures made of regenerated silk proteins. Adv Mater, 2021, 33(1): e2004733. doi: 10.1002/adma.202004733.
51.	Jin D, Hu J, Xia D, et al. Evaluation of a simple off-the-shelf bi-layered vascular scaffold based on poly(L-lactide-co-ε-caprolactone)/silk fibroin in vitro and in vivo. Int J Nanomedicine, 2019, 14: 4261-4276.
52.	Liu Z, Zheng Z, Chen K, et al. A heparin-functionalized woven stent graft for endovascular exclusion. Colloids Surf B Biointerfaces, 2019, 180: 118-126.

1. Huang W, Ling S, Li C, et al. Silkworm silk-based materials and devices generated using bio-nanotechnology. Chem Soc Rev, 2018, 47(17): 6486-6504.
2. Tomeh MA, Hadianamrei R, Zhao X. Silk fibroin as a functional biomaterial for drug and gene delivery. Pharmaceutics, 2019, 11(10): 494. doi: 10.3390/pharmaceutics11100494.
3. Tran SH, Wilson CG, Seib FP. A review of the emerging role of silk for the treatment of the eye. Pharm Res, 2018, 35(12): 248. doi: 10.1007/s11095-018-2534-y.
4. Wani SUD, Gautam SP, Qadrie ZL, et al. Silk fibroin as a natural polymeric based bio-material for tissue engineering and drug delivery systems-A review. Int J Biol Macromol, 2020, 163: 2145-2161.
5. Farokhi M, Mottaghitalab F, Reis RL, et al. Functionalized silk fibroin nanofibers as drug carriers: Advantages and challenges. J Control Release, 2020, 321: 324-347.
6. Zheng H, Zuo B. Functional silk fibroin hydrogels: preparation, properties and applications. J Mater Chem B, 2021, 9(5): 1238-1258.
7. Quartinello F, Tallian C, Auer J, et al. Smart textiles in wound care: functionalization of cotton/PET blends with antimicrobial nanocapsules. J Mater Chem B, 2019, 7(42): 6592-6603.
8. Tallian C, Herrero-Rollett A, Stadler K, et al. Structural insights into pH-responsive drug release of self-assembling human serum albumin-silk fibroin nanocapsules. Eur J Pharm Biopharm, 2018, 133: 176-187.
9. Yonesi M, Garcia-Nieto M, Guinea GV, et al. Silk fibroin: An ancient material for repairing the injured nervous system. Pharmaceutics, 2021, 13(3): 429. doi: 10.3390/pharmaceutics13030429.
10. Galam N, Tulay P, Adali T. In vitro MCF-7 cells apoptosis analysis of carboplatin loaded silk fibroin particles. Molecules, 2020, 25(5): 1110. doi: 10.3390/molecules25051110.
11. Shen X, Zhang Y, Gu Y, et al. Sequential and sustained release of SDF-1 and BMP-2 from silk fibroin-nanohydroxyapatite scaffold for the enhancement of bone regeneration. Biomaterials, 2016, 106: 205-216.
12. Sharma S, Bano S, Ghosh AS, et al. Silk fibroin nanoparticles support in vitro sustained antibiotic release and osteogenesis on titanium surface. Nanomedicine, 2016, 12(5): 1193-1204.
13. Zhao X, Chen Z, Liu Y, et al. Silk fibroin microparticles with hollow mesoporous silica nanocarriers encapsulation for abdominal wall repair. Adv Healthc Mater, 2018, 7(21): e1801005. doi: 10.1002/adhm.201801005.
14. Luo J, Zhang H, Zhu J, et al. 3-D mineralized silk fibroin/polycaprolactone composite scaffold modified with polyglutamate conjugated with BMP-2 peptide for bone tissue engineering. Colloids Surf B Biointerfaces, 2018, 163: 369-378.
15. Zhang L, Xu L, Li G, et al. Fabrication of high-strength mecobalamin loaded aligned silk fibroin scaffolds for guiding neuronal orientation. Colloids Surf B Biointerfaces, 2019, 173: 689-697.
16. Xu R, Zhang Z, Toftdal MS, et al. Synchronous delivery of hydroxyapatite and connective tissue growth factor derived osteoinductive peptide enhanced osteogenesis. J Control Release, 2019, 301: 129-139.
17. Ou L, Lan Y, Feng Z, et al. Functionalization of SF/HAP scaffold with GO-PEI-miRNA inhibitor complexes to enhance bone regeneration through activating transcription factor 4. Theranostics, 2019, 9(15): 4525-4541.
18. Yang Q, Teng BH, Wang LN, et al. Silk fibroin/cartilage extracellular matrix scaffolds with sequential delivery of TGF-β₃ for chondrogenic differentiation of adipose-derived stem cells. Int J Nanomedicine, 2017, 12: 6721-6733.
19. Wang Q, Cao L, Liu Y, et al. Evaluation of synergistic osteogenesis between icariin and BMP2 through a micro/meso hierarchical porous delivery system. Int J Nanomedicine, 2017, 12: 7721-7735.
20. Xin X, Wu J, Zheng A, et al. Delivery vehicle of muscle-derived irisin based on silk/calcium silicate/sodium alginate composite scaffold for bone regeneration. Int J Nanomedicine, 2019, 14: 1451-1467.
21. Shera SS, Sahu S, Banik RM. Preparation of drug eluting natural composite scaffold using response surface methodology and artificial neural network approach. Tissue Eng Regen Med, 2018, 15(2): 131-143.
22. 黄文良, 叶鹏, 莫刚, 等. 制备缓释骨形态发生蛋白2的丝素蛋白/壳聚糖/纳米羟基磷灰石生物支架. 中国组织工程研究, 2017, 21(22): 3488-3493.
23. Ramadass SK, Nazir LS, Thangam R, et al. Type Ⅰ collagen peptides and nitric oxide releasing electrospun silk fibroin scaffold: A multifunctional approach for the treatment of ischemic chronic wounds. Colloids Surf B Biointerfaces, 2019, 175: 636-643.
24. Li M, Ai M, Yang Y, et al. Silk-coated dexamethasone non-spherical microcrystals for local drug delivery to inner ear. Eur J Pharm Sci, 2020, 150: 105336. doi: 10.1016/j.ejps.2020.105336.
25. Li G, Che MT, Zhang K, et al. Graft of the NT-3 persistent delivery gelatin sponge scaffold promotes axon regeneration, attenuates inflammation, and induces cell migration in rat and canine with spinal cord injury. Biomaterials, 2016, 83: 233-248.
26. Li G, Che MT, Zeng X, et al. Neurotrophin-3 released from implant of tissue-engineered fibroin scaffolds inhibits inflammation, enhances nerve fiber regeneration, and improves motor function in canine spinal cord injury. J Biomed Mater Res A, 2018, 106(8): 2158-2170.
27. Bhattacharjee P, Naskar D, Maiti TK, et al. Non-mulberry silk fibroin grafted poly (Є-caprolactone)/nano hydroxyapatite nanofibrous scaffold for dual growth factor delivery to promote bone regeneration. J Colloid Interface Sci, 2016, 472: 16-33.
28. Zhou WH, Zhang T, Yan JL, et al. In vitro and in vivo evaluation of structurally-controlled silk fibroin coatings for orthopedic infection and in-situ osteogenesis. Acta Biomater, 2020, 116: 223-245.
29. Xiao W, Zhang J, Qu X, et al. Fabrication of protease ⅩⅣ-loaded microspheres for cell spreading in silk fibroin hydrogels. J Mater Sci Mater Med, 2020, 31(12): 128. doi: 10.1007/s10856-020-06466-7.
30. Shao J, Ding Z, Li L, et al. Improved accumulation of TGF-β by photopolymerized chitosan/silk protein bio-hydrogel matrix to improve differentiations of mesenchymal stem cells in articular cartilage tissue regeneration. J Photochem Photobiol B, 2020, 203: 111744. doi: 10.1016/j.jphotobiol.2019.111744.
31. Ziadlou R, Rotman S, Teuschl A, et al. Optimization of hyaluronic acid-tyramine/silk-fibroin composite hydrogels for cartilage tissue engineering and delivery of anti-inflammatory and anabolic drugs. Mater Sci Eng C Mater Biol Appl, 2021, 120: 111701. doi: 10.1016/j.msec.2020.111701.
32. He S, Shi D, Han Z, et al. Heparinized silk fibroin hydrogels loading FGF1 promote the wound healing in rats with full-thickness skin excision. Biomed Eng Online, 2019, 18(1): 97. doi: 10.1186/s12938-019-0716-4.
33. Oliveira IM, Gonçalves C, Shin ME, et al. Anti-inflammatory properties of injectable betamethasone-loaded tyramine-modified gellan gum/silk fibroin hydrogels. Biomolecules, 2020, 10(10): 1456. doi: 10.3390/biom10101456.
34. Laomeephol C, Ferreira H, Kanokpanont S, et al. Dual-functional liposomes for curcumin delivery and accelerating silk fibroin hydrogel formation. Int J Pharm, 2020, 589: 119844. doi: 10.1016/j.ijpharm.2020.119844.
35. Youn J, Choi JH, Lee S, et al. Pluronic F-127/silk fibroin for enhanced mechanical property and sustained release drug for tissue engineering biomaterial. Materials (Basel), 2021, 14(5): 1287. doi: 10.3390/ma14051287.
36. Yan S, Wang Q, Tariq Z, et al. Facile preparation of bioactive silk fibroin/hyaluronic acid hydrogels. Int J Biol Macromol, 2018, 118(Pt A): 775-782.
37. Wu J, Zheng K, Huang X, et al. Thermally triggered injectable chitosan/silk fibroin/bioactive glass nanoparticle hydrogels for in-situ bone formation in rat calvarial bone defects. Acta Biomater, 2019, 91: 60-71.
38. Xu X, Wang X, Qin C, et al. Silk fibroin/poly-(L-lactide-co-caprolactone) nanofiber scaffolds loaded with Huangbai Liniment to accelerate diabetic wound healing. Colloids Surf B Biointerfaces, 2021, 199: 111557. doi: 10.1016/j.colsurfb.2021.111557.
39. Hadisi Z, Farokhi M, Bakhsheshi-Rad HR, et al. Hyaluronic acid (HA)-based silk fibroin/zinc oxide core-shell electrospun dressing for burn wound management. Macromol Biosci, 2020, 20(4): e1900328. doi: 10.1002/mabi.201900328.
40. Wang Z, Song X, Cui Y, et al. Silk fibroin H-fibroin/poly(ε-caprolactone) core-shell nanofibers with enhanced mechanical property and long-term drug release. J Colloid Interface Sci, 2021, 593: 142-151.
41. Khan AUR, Nadeem M, Bhutto MA, et al. Physico-chemical and biological evaluation of PLCL/SF nanofibers loaded with oregano essential oil. Pharmaceutics, 2019, 11(8): 386. doi: 10.3390/pharmaceutics11080386.
42. Dadras Chomachayi M, Solouk A, Akbari S, et al. Electrospun nanofibers comprising of silk fibroin/gelatin for drug delivery applications: Thyme essential oil and doxycycline monohydrate release study. J Biomed Mater Res A, 2018, 106(4): 1092-1103.
43. Chouhan D, Chakraborty B, Nandi SK, et al. Role of non-mulberry silk fibroin in deposition and regulation of extracellular matrix towards accelerated wound healing. Acta Biomater, 2017, 48: 157-174.
44. Song J, Klymov A, Shao J, et al. Electrospun nanofibrous silk fibroin membranes containing gelatin nanospheres for controlled delivery of biomolecules. Adv Healthc Mater, 2017, 6(14). doi: 10.1002/adhm.201700014.
45. Kalani MM, Nourmohammadi J, Negahdari B, et al. Electrospun core-sheath poly (vinyl alcohol)/silk fibroin nanofibers with Rosuvastatin release functionality for enhancing osteogenesis of human adipose-derived stem cells. Mater Sci Eng C Mater Biol Appl, 2019, 99: 129-139.
46. Xu R, Zhao H, Muhammad H, et al. Dual-delivery of FGF-2/CTGF from silk fibroin/PLCL-PEO coaxial fibers enhances MSC proliferation and fibrogenesis. Sci Rep, 2017, 7(1): 8509. doi: 10.1038/s41598-017-08226-0.
47. Silva SS, Oliveira NM, Oliveira MB, et al. Fabrication and characterization of Eri silk fibers-based sponges for biomedical application. Acta Biomater, 2016, 32: 178-189.
48. Wang Q, Qian Z, Liu B, et al. In vitro and in vivo evaluation of new PRP antibacterial moisturizing dressings for infectious wound repair. J Biomater Sci Polym Ed, 2019, 30(6): 462-485.
49. Naskar D, Ghosh AK, Mandal M, et al. Dual growth factor loaded nonmulberry silk fibroin/carbon nanofiber composite 3D scaffolds for in vitro and in vivo bone regeneration. Biomaterials, 2017, 136: 67-85.
50. Liu M, Zhang Y, Liu K, et al. Biomimicking antibacterial opto-electro sensing sutures made of regenerated silk proteins. Adv Mater, 2021, 33(1): e2004733. doi: 10.1002/adma.202004733.
51. Jin D, Hu J, Xia D, et al. Evaluation of a simple off-the-shelf bi-layered vascular scaffold based on poly(L-lactide-co-ε-caprolactone)/silk fibroin in vitro and in vivo. Int J Nanomedicine, 2019, 14: 4261-4276.
52. Liu Z, Zheng Z, Chen K, et al. A heparin-functionalized woven stent graft for endovascular exclusion. Colloids Surf B Biointerfaces, 2019, 180: 118-126.

Chinese Journal of Reparative and Reconstructive Surgery

Progress in the application of silk fibroin in tissue engineered drug delivery system

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content