Near-infrared excited graphene oxide/silver nitrate/chitosan coating for improving antibacterial properties of titanium implants_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

WANG Yifan ¹ , XU Yingde ¹ , ZHANG Xuefeng ¹ , LIU Jingyu ¹ , HAN Jintong ¹ , ZHU Shengli ¹ , LIANG Yanqin ¹ , WU Shuilin ¹ , CUI Zhenduo ¹ , LÜ Weijia ² ,  LI Zhaoyang ¹

1. Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science and Engineering, Tianjin University, Tianjin, 300350, P. R. China;
2. Faculty of Pharmaceutical Sciences, Shenzhen Institute of Advanced Technology Chinese Academy of Sciences, Shenzhen Guangdong, 518055, P. R. China;

Corresponding author：

LI Zhaoyang, Email: zyli@tju.edu.cn

Keywords：

Bacterial infection; antibacterial coating; heterojunction; photothermal-photodynamic antibacterial

DOI：

10.7507/1002-1892.202303041

Video：

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Objective To design and construct a graphene oxide (GO)/silver nitrate (Ag₃PO₄)/chitosan (CS) composite coating for rapidly killing bacteria and preventing postoperative infection in implant surgery. Methods GO/Ag₃PO₄ composites were prepared by ion exchange method, and CS and GO/Ag₃PO₄ composites were deposited on medical titanium (Ti) sheets successively. The morphology, physical image, photothermal and photocatalytic ability, antibacterial ability, and adhesion to the matrix of the materials were characterized. Results The GO/Ag₃PO₄ composites were successfully prepared by ion exchange method and the heterogeneous structure of GO/Ag₃PO₄ was proved by morphology phase test. The heterogeneous structure formed by Ag₃PO₄ and GO reduced the band gap from 1.79 eV to 1.39 eV which could be excited by 808 nm near-infrared light. The photothermal and photocatalytic experiments proved that the GO/Ag₃PO₄/CS coating had excellent photothermal and photodynamic properties. In vitro antibacterial experiments showed that the antibacterial rate of the GO/Ag₃PO₄/CS composite coating against Staphylococcus aureus reached 99.81% after 20 minutes irradiation with 808 nm near-infrared light. At the same time, the composite coating had excellent light stability, which could provide stable and sustained antibacterial effect. Conclusion GO/Ag₃PO₄/CS coating can be excited by 808 nm near infrared light to produce reactive oxygen species, which has excellent antibacterial activity under light.

Citation： WANG Yifan, XU Yingde, ZHANG Xuefeng, LIU Jingyu, HAN Jintong, ZHU Shengli, LIANG Yanqin, WU Shuilin, CUI Zhenduo, LÜ Weijia, LI Zhaoyang. Near-infrared excited graphene oxide/silver nitrate/chitosan coating for improving antibacterial properties of titanium implants. Chinese Journal of Reparative and Reconstructive Surgery, 2023, 37(8): 937-944. doi: 10.7507/1002-1892.202303041 Copy

1.	Tan L, Li J, Liu X, et al. Rapid biofilm eradication on bone implants using red phosphorus and near-infrared light. Adv Mater, 2018, 30(31): e1801808.
2.	张超群, 吴一芃, 徐永清. 骨科内植物表面抗菌改性的研究进展. 中国修复重建外科杂志, 2022, 36(4): 511-516.
3.	Li J, Li Z, Liu X, et al. Interfacial engineering of Bi2S3/Ti3C2Tx MXene based on work function for rapid photo-excited bacteria-killing. Nat Commun, 2021, 12(1): 1224.
4.	丁思勰, 洪慧蕾, 徐凌寒, 等. 酚胺交联涂层接枝氯己定改善钛表面抗菌性能研究. 中国修复重建外科杂志, 2022, 36(3): 335-342.
5.	何宏燕, 郜彭阳, 乔忠乾, 等. 钛金属表面微纳结构协同抗菌肽的抗菌性能研究. 中国修复重建外科杂志, 2018, 32(9): 1116-1122.
6.	Hong L, Liu X, Tan L, et al. Rapid biofilm elimination on bone implants using near-infrared-activated inorganic semiconductor heterostructures. Adv Healthc Mater, 2019, 8(19): e1900835.
7.	陈媛媛, 唐晓宁, 崔帅, 等. 活性氧抗菌机理及其研究进展. 工程科学学报, 2023, 45(6): 967-978.
8.	Wang Y, Xu Y, Xia H, et al. Near-infrared light-responsive MnOx/Ag₃PO₄ heterojunction for rapidly eradicating sternal fracture implant infection. Colloid Interface Sci Commun, 2022, 51: 100679.
9.	Li J, Tian X, Hua T, et al. Chitosan natural polymer material for improving antibacterial properties of textiles. ACS Appl Bio Mater, 2021, 4(5): 4014-4038.
10.	Wang X, Su K, Tan L, et al. Rapid and highly effective noninvasive disinfection by hybrid Ag/CS@MnO₂ nanosheets using near-infrared light. ACS Appl Mater Interfaces, 2019, 11(16): 15014-15027.
11.	Xie X, Mao C, Liu X, et al. Synergistic bacteria killing through photodynamic and physical actions of graphene oxide/Ag/collagen coating. ACS Appl Mater Interfaces, 2017, 9(31): 26417-26428.
12.	Li M, Chen Z, Sun Y, et al. Preparation and antibacterial activity of graphene oxide/cuprous oxide/zinc oxide nanocomposite. Materials Research Express, 2021, 8(12): 125003.
13.	AlSalhi MS, Devanesan S, Asemi NN, et al. Construction of SnO₂/CuO/rGO nanocomposites for photocatalytic degradation of organic pollutants and antibacterial applications. Environ Res, 2023, 222: 115370.
14.	Zhang Q, Liu X, Tan L, et al. An UV to NIR-driven platform based on red phosphorus/graphene oxide film for rapid microbial inactivation. Chem Eng J, 2020, 383: 123088.
15.	Kichukova D, Spassova I, Kostadinova A, et al. Facile synthesized Cu-RGO and Ag-RGO nanocomposites with potential biomedical applications. Nanomaterials (Basel), 2022, 12(12): 2096.
16.	Shalini A, Priya K, Kothai S, et al. Synthesis and characteri-sation of graphene oxide decorated gold nano particles and their application towards antibacterial activity. Chem Pap, 2022, 76(11): 6861-6867.
17.	Su W, Liu X, Tan L, et al. Rapid sterilization by photocatalytic Ag₃PO₄/α-Fe₂O₃ composites using visible light. ACS Sustainable Chem Eng, 2020, 8(6): 2577-2585.
18.	Xu Y, Liu X, Zheng Y, et al. Ag₃PO₄ decorated black urchin-like defective TiO₂ for rapid and long-term bacteria-killing under visible light. Bioact Mater, 2020, 6(6): 1575-1587.
19.	姜柳, 高琴, 王震宇, 等. 有机阳离子型电荷杀菌材料研究现状及展望. 地球环境学报, 2022, 13(6): 679-701.
20.	Xie X, Mao C, Liu X, et al. Tuning the bandgap of photo-sensitive polydopamine/Ag₃PO₄/graphene oxide coating for rapid, noninvasive disinfection of implants. ACS Cent Sci, 2018, 4(6): 724-738.
21.	Rojas Daniel, Kuthanova Michaela, Dolezelikova Kristyna, et al. Facet nanoarchitectonics of visible-light driven Ag₃PO₄ photocatalytic micromotors: Tuning motion for biofilm eradication. NPG Asia Mater, 2022, 14(1). doi: 10.1038/s41427-022-00409-0.
22.	Nicolaou KC, Chen JS, Edmonds DJ, et al. Recent advances in the chemistry and biology of naturally occurring antibiotics. Angew Chem Int Ed Engl, 2009, 48(4): 660-719.
23.	Neoh KG, Hu X, Zheng D, et al. Balancing osteoblast functions and bacterial adhesion on functionalized titanium surfaces. Biomaterials, 2012, 33(10): 2813-2822.
24.	张若男, 吴迪, 高艺恬. 抗菌肽的设计与优化研究进展. 生物医学工程学杂志, 2022, 39(6): 1247-1253.
25.	Shibu Edakkattuparambil Sidharth, Hamada Morihiko, Murase Norio, et al. Nanomaterials formulations for photothermal and photodynamic therapy of cancer. J Photochem Photobiol C, 2013, 15: 53-72.
26.	Chen W, Chang H, Lu J, et al. Self-assembly of antimicrobial peptides on gold nanodots: Against multidrug-resistant bacteria and wound-healing application. Adv Funct Mater, 2015, 25(46): 7189-7199.
27.	Shao N, Wang J, Wang D, et al. Preparation of three-dimensional Ag₃PO₄/TiO₂@MoS₂ for enhanced visible-light photocatalytic activity and anti-photocorrosion. Appl Catal B, 2017, 203: 964-978.
28.	Muthukumar Pandi, Alex Vinotha, Pannipara Mehboobali, et al. Fabricating highly efficient Ag₃PO₄-Fe₃O₄-GO ternary nanocomposite photocatalyst: Effect of Fe₃O₄-GO preparation methods on photocatalytic activity. Materials Research Bulletin, 2021, 141: 111337.
29.	Xia MY, Xie Y, Yu CH, et al. Graphene-based nanomaterials: the promising active agents for antibiotics-independent antibacterial applications. J Control Release, 2019, 307: 16-31.
30.	Wang L, Gao F, Wang A, et al. Defect-rich adhesive molybdenum disulfide/rGO vertical heterostructures with enhanced nanozyme activity for smart bacterial killing application. Adv Mater, 2020, 32(48): e2005423.
31.	Liu G, Zhao X, Zhang J, et al. Z-scheme Ag₃PO₄/POM/GO heterojunction with enhanced photocatalytic performance for degradation and water splitting. Dalton Trans, 2018, 47(17): 6225-6232.

1. Tan L, Li J, Liu X, et al. Rapid biofilm eradication on bone implants using red phosphorus and near-infrared light. Adv Mater, 2018, 30(31): e1801808.
2. 张超群, 吴一芃, 徐永清. 骨科内植物表面抗菌改性的研究进展. 中国修复重建外科杂志, 2022, 36(4): 511-516.
3. Li J, Li Z, Liu X, et al. Interfacial engineering of Bi2S3/Ti3C2Tx MXene based on work function for rapid photo-excited bacteria-killing. Nat Commun, 2021, 12(1): 1224.
4. 丁思勰, 洪慧蕾, 徐凌寒, 等. 酚胺交联涂层接枝氯己定改善钛表面抗菌性能研究. 中国修复重建外科杂志, 2022, 36(3): 335-342.
5. 何宏燕, 郜彭阳, 乔忠乾, 等. 钛金属表面微纳结构协同抗菌肽的抗菌性能研究. 中国修复重建外科杂志, 2018, 32(9): 1116-1122.
6. Hong L, Liu X, Tan L, et al. Rapid biofilm elimination on bone implants using near-infrared-activated inorganic semiconductor heterostructures. Adv Healthc Mater, 2019, 8(19): e1900835.
7. 陈媛媛, 唐晓宁, 崔帅, 等. 活性氧抗菌机理及其研究进展. 工程科学学报, 2023, 45(6): 967-978.
8. Wang Y, Xu Y, Xia H, et al. Near-infrared light-responsive MnOx/Ag₃PO₄ heterojunction for rapidly eradicating sternal fracture implant infection. Colloid Interface Sci Commun, 2022, 51: 100679.
9. Li J, Tian X, Hua T, et al. Chitosan natural polymer material for improving antibacterial properties of textiles. ACS Appl Bio Mater, 2021, 4(5): 4014-4038.
10. Wang X, Su K, Tan L, et al. Rapid and highly effective noninvasive disinfection by hybrid Ag/CS@MnO₂ nanosheets using near-infrared light. ACS Appl Mater Interfaces, 2019, 11(16): 15014-15027.
11. Xie X, Mao C, Liu X, et al. Synergistic bacteria killing through photodynamic and physical actions of graphene oxide/Ag/collagen coating. ACS Appl Mater Interfaces, 2017, 9(31): 26417-26428.
12. Li M, Chen Z, Sun Y, et al. Preparation and antibacterial activity of graphene oxide/cuprous oxide/zinc oxide nanocomposite. Materials Research Express, 2021, 8(12): 125003.
13. AlSalhi MS, Devanesan S, Asemi NN, et al. Construction of SnO₂/CuO/rGO nanocomposites for photocatalytic degradation of organic pollutants and antibacterial applications. Environ Res, 2023, 222: 115370.
14. Zhang Q, Liu X, Tan L, et al. An UV to NIR-driven platform based on red phosphorus/graphene oxide film for rapid microbial inactivation. Chem Eng J, 2020, 383: 123088.
15. Kichukova D, Spassova I, Kostadinova A, et al. Facile synthesized Cu-RGO and Ag-RGO nanocomposites with potential biomedical applications. Nanomaterials (Basel), 2022, 12(12): 2096.
16. Shalini A, Priya K, Kothai S, et al. Synthesis and characteri-sation of graphene oxide decorated gold nano particles and their application towards antibacterial activity. Chem Pap, 2022, 76(11): 6861-6867.
17. Su W, Liu X, Tan L, et al. Rapid sterilization by photocatalytic Ag₃PO₄/α-Fe₂O₃ composites using visible light. ACS Sustainable Chem Eng, 2020, 8(6): 2577-2585.
18. Xu Y, Liu X, Zheng Y, et al. Ag₃PO₄ decorated black urchin-like defective TiO₂ for rapid and long-term bacteria-killing under visible light. Bioact Mater, 2020, 6(6): 1575-1587.
19. 姜柳, 高琴, 王震宇, 等. 有机阳离子型电荷杀菌材料研究现状及展望. 地球环境学报, 2022, 13(6): 679-701.
20. Xie X, Mao C, Liu X, et al. Tuning the bandgap of photo-sensitive polydopamine/Ag₃PO₄/graphene oxide coating for rapid, noninvasive disinfection of implants. ACS Cent Sci, 2018, 4(6): 724-738.
21. Rojas Daniel, Kuthanova Michaela, Dolezelikova Kristyna, et al. Facet nanoarchitectonics of visible-light driven Ag₃PO₄ photocatalytic micromotors: Tuning motion for biofilm eradication. NPG Asia Mater, 2022, 14(1). doi: 10.1038/s41427-022-00409-0.
22. Nicolaou KC, Chen JS, Edmonds DJ, et al. Recent advances in the chemistry and biology of naturally occurring antibiotics. Angew Chem Int Ed Engl, 2009, 48(4): 660-719.
23. Neoh KG, Hu X, Zheng D, et al. Balancing osteoblast functions and bacterial adhesion on functionalized titanium surfaces. Biomaterials, 2012, 33(10): 2813-2822.
24. 张若男, 吴迪, 高艺恬. 抗菌肽的设计与优化研究进展. 生物医学工程学杂志, 2022, 39(6): 1247-1253.
25. Shibu Edakkattuparambil Sidharth, Hamada Morihiko, Murase Norio, et al. Nanomaterials formulations for photothermal and photodynamic therapy of cancer. J Photochem Photobiol C, 2013, 15: 53-72.
26. Chen W, Chang H, Lu J, et al. Self-assembly of antimicrobial peptides on gold nanodots: Against multidrug-resistant bacteria and wound-healing application. Adv Funct Mater, 2015, 25(46): 7189-7199.
27. Shao N, Wang J, Wang D, et al. Preparation of three-dimensional Ag₃PO₄/TiO₂@MoS₂ for enhanced visible-light photocatalytic activity and anti-photocorrosion. Appl Catal B, 2017, 203: 964-978.
28. Muthukumar Pandi, Alex Vinotha, Pannipara Mehboobali, et al. Fabricating highly efficient Ag₃PO₄-Fe₃O₄-GO ternary nanocomposite photocatalyst: Effect of Fe₃O₄-GO preparation methods on photocatalytic activity. Materials Research Bulletin, 2021, 141: 111337.
29. Xia MY, Xie Y, Yu CH, et al. Graphene-based nanomaterials: the promising active agents for antibiotics-independent antibacterial applications. J Control Release, 2019, 307: 16-31.
30. Wang L, Gao F, Wang A, et al. Defect-rich adhesive molybdenum disulfide/rGO vertical heterostructures with enhanced nanozyme activity for smart bacterial killing application. Adv Mater, 2020, 32(48): e2005423.
31. Liu G, Zhao X, Zhang J, et al. Z-scheme Ag₃PO₄/POM/GO heterojunction with enhanced photocatalytic performance for degradation and water splitting. Dalton Trans, 2018, 47(17): 6225-6232.

Chinese Journal of Reparative and Reconstructive Surgery

Near-infrared excited graphene oxide/silver nitrate/chitosan coating for improving antibacterial properties of titanium implants

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