Experimental Study on PHBHHx Co-culturing with Mouse Induced Pluripotent Stem Cells outside Body_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

XUShi-jun ,  MUJun-sheng , ZHANGJian-qun , BOPing

Department of Cardiac Surgery, Beijing Anzhen Hospital, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Capital Medical University, Beijing 100029, P. R. China;

Corresponding author：

MUJun-sheng, Email: wesleymu@hotmail.com

Keywords：

Mouse induced pluripotent stem cells; Myocardial Patches; Poly-3-hydroxybutyrate-co-3-hydroxyhex-anoate(PHBHHx)film; Stem cell therapy

DOI：

10.7507/1007-4848.20160196

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To study the external biocompatibility bewteen the mouse induced pluripotent stem cells (miPSCs) and poly-3-hydroxybutyrate-co-3-hydroxyhexanoate (PHBHHx). Methods After we recovered and subcultured miPSCs, we divided them into two groups. There was one group cultured with material of PHBHHx films outside the body. We observed the adhesive pattern of miPSCs on film by fluorescence of 4, 6-diamidino-2-phenylindole (DAPI) staining. The cell vitality was detected by cell counting kit-8 (CCK-8). The morphology of miPSCs attached on the film was visualized under scanning electron microscope (SEM). We used the traditional petri dish to culture miPSCs and detect the cell activity by CCK-8. Results MiPSCs can adhere and proliferate on PHBHHx films. The result of cell vitality which detected by CCK-8 showed that there was a statistical difference in OD value between culturing on PHBHHx films and traditional cultivation (0.617±0.019 vs. 0.312±0.004, P < 0.05). Conclusion There are adhesion and proliferation on the surface of cells patch made by miPSCs co-culturing with PHBHHx film. Compared with traditional culturing in the cell culture dish, culturing in PHBHHx films have great advantages in the process of adhesion and proliferation. PHBHHx can be used as one of the scaffold for stem cells treating various disease.

Citation： XUShi-jun, MUJun-sheng, ZHANGJian-qun, BOPing. Experimental Study on PHBHHx Co-culturing with Mouse Induced Pluripotent Stem Cells outside Body. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2016, 23(8): 822-826. doi: 10.7507/1007-4848.20160196 Copy

1.	穆军升, 李献帅, 袁树民, 等.直接贴壁法诱导人胚胎干细胞分化为心肌细胞.中国胸心血管外科临床杂志, 2013, 20(5):564-569.
2.	李献帅, 袁树民, 穆军升, 等.悬浮培养诱导人胚胎干细胞分化为心肌细胞的研究.心肺血管病杂志, 2014, 33(1):93-97.
3.	Kovacic JC, Fuster V. Cell therapy for patients with acute myocar-dial infarction:accured evidence to date. Circ Res, 2015, 116(8):1287-1290.
4.	Al KA, Asenjo JF, Ge Y, et al. Microencapsulation to reduce mecha-nical loss of microspheres:implications in myocardial cell therapy. Eur J Cardiothorac Surg, 2011, 39(2):241-247.
5.	Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell, 2006, 126(4):663-676.
6.	Yoshida Y, Yamanaka S. iPS cells:a source of cardiac regeneration. J Mol Cell Cardiol, 2011, 50(2):327-332.
7.	牛红星, 穆军升, 张健群, 等.骨髓间充质干细胞与聚3-羟基丁酸酯-co-4-羟基丁酸酯生物材料共培养细胞补片的初步研究.中国生物工程杂志, 2012, 32(10):74-79.
8.	Yu H, Wooley PH, Yang SY. Biocompatibility of Poly-epsilon-capro-lactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells. J Orthop Surg Res, 2009, 4(4):5.
9.	Jain K, Verma PJ, Liu J. Isolation and handling of mouse embryonic fibroblasts. Methods Mol Biol, 2014, 1194(6):247-252.
10.	吕海侠, 杨志倩, 卢晓云, 等. PHBHHx膜表面亲水改性及其与神经干细胞生物相容性.生物工程学报, 2012, 28(10):1216-1226.
11.	Matar AA, Chong JJ. Stem cell therapy for cardiac dysfunction. Springerplus, 2014, 3(3):440.
12.	刘丽, 张海波.诱导多能干细胞体外分化为心肌细胞研究现状.中华临床医师杂志(电子版), 2012, 28(10):1216-1226.
13.	Yu J, Vodyanik MA, Smuga-Otto K, et al. Induced pluripotent stem cell lines derived from human somatic cells. Science, 2007, 318(5858):1917-1920.
14.	Eminli S, Utikal J, Arnold K, et al. Reprogramming of neural progenitor cells into induced pluripotent stem cells in the absence of exogenous Sox2 expression. Stem Cells, 2008, 26(10):2467-2474.
15.	Nelson TJ, Martinez-Fernandez A, Yamada S, et al. Repair of acute myocardial infarction by human stemness factors induced pluripotent stem cells. Circulation, 2009, 120(5):408-416.
16.	Li X, Zhang F, Song G, et al. Intramyocardial injection of pig pluri-potent stem cells improves left ventricular function and perfusion:A dtudy in a porcine model of scute myocardial infarction. PLoS One, 2013, 8(6):e66688.
17.	Al KA, Asenjo JF, Ge Y, et al. Microencapsulation to reduce mecha-nical loss of microspheres:implications in myocardial cell therapy. Eur J Cardiothorac Surg, 2011, 39(2):241-247.
18.	Wu S, Liu Y, Cui B, et al. Intravascular biocompatibility of decellu-larized xenogenic vascular scaffolds/PHBHHx hybrid material for cardiovascular tissue engineering. Sheng Wu Gong Cheng Xue Bao, 2008, 24(4):610-616.
19.	Chen GQ, Wu Q. The application of polyhydroxyalkanoates as tissue engineering materials. Biomaterials, 2005, 26(33):6565-6578.
20.	Yu H, Wooley PH, Yang SY. Biocompatibility of Poly-epsilon-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells. J Orthop Surg Res, 2009, 4(6):5.

1. 穆军升, 李献帅, 袁树民, 等.直接贴壁法诱导人胚胎干细胞分化为心肌细胞.中国胸心血管外科临床杂志, 2013, 20(5):564-569.
2. 李献帅, 袁树民, 穆军升, 等.悬浮培养诱导人胚胎干细胞分化为心肌细胞的研究.心肺血管病杂志, 2014, 33(1):93-97.
3. Kovacic JC, Fuster V. Cell therapy for patients with acute myocar-dial infarction:accured evidence to date. Circ Res, 2015, 116(8):1287-1290.
4. Al KA, Asenjo JF, Ge Y, et al. Microencapsulation to reduce mecha-nical loss of microspheres:implications in myocardial cell therapy. Eur J Cardiothorac Surg, 2011, 39(2):241-247.
5. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell, 2006, 126(4):663-676.
6. Yoshida Y, Yamanaka S. iPS cells:a source of cardiac regeneration. J Mol Cell Cardiol, 2011, 50(2):327-332.
7. 牛红星, 穆军升, 张健群, 等.骨髓间充质干细胞与聚3-羟基丁酸酯-co-4-羟基丁酸酯生物材料共培养细胞补片的初步研究.中国生物工程杂志, 2012, 32(10):74-79.
8. Yu H, Wooley PH, Yang SY. Biocompatibility of Poly-epsilon-capro-lactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells. J Orthop Surg Res, 2009, 4(4):5.
9. Jain K, Verma PJ, Liu J. Isolation and handling of mouse embryonic fibroblasts. Methods Mol Biol, 2014, 1194(6):247-252.
10. 吕海侠, 杨志倩, 卢晓云, 等. PHBHHx膜表面亲水改性及其与神经干细胞生物相容性.生物工程学报, 2012, 28(10):1216-1226.
11. Matar AA, Chong JJ. Stem cell therapy for cardiac dysfunction. Springerplus, 2014, 3(3):440.
12. 刘丽, 张海波.诱导多能干细胞体外分化为心肌细胞研究现状.中华临床医师杂志(电子版), 2012, 28(10):1216-1226.
13. Yu J, Vodyanik MA, Smuga-Otto K, et al. Induced pluripotent stem cell lines derived from human somatic cells. Science, 2007, 318(5858):1917-1920.
14. Eminli S, Utikal J, Arnold K, et al. Reprogramming of neural progenitor cells into induced pluripotent stem cells in the absence of exogenous Sox2 expression. Stem Cells, 2008, 26(10):2467-2474.
15. Nelson TJ, Martinez-Fernandez A, Yamada S, et al. Repair of acute myocardial infarction by human stemness factors induced pluripotent stem cells. Circulation, 2009, 120(5):408-416.
16. Li X, Zhang F, Song G, et al. Intramyocardial injection of pig pluri-potent stem cells improves left ventricular function and perfusion:A dtudy in a porcine model of scute myocardial infarction. PLoS One, 2013, 8(6):e66688.
17. Al KA, Asenjo JF, Ge Y, et al. Microencapsulation to reduce mecha-nical loss of microspheres:implications in myocardial cell therapy. Eur J Cardiothorac Surg, 2011, 39(2):241-247.
18. Wu S, Liu Y, Cui B, et al. Intravascular biocompatibility of decellu-larized xenogenic vascular scaffolds/PHBHHx hybrid material for cardiovascular tissue engineering. Sheng Wu Gong Cheng Xue Bao, 2008, 24(4):610-616.
19. Chen GQ, Wu Q. The application of polyhydroxyalkanoates as tissue engineering materials. Biomaterials, 2005, 26(33):6565-6578.
20. Yu H, Wooley PH, Yang SY. Biocompatibility of Poly-epsilon-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells. J Orthop Surg Res, 2009, 4(6):5.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Experimental Study on PHBHHx Co-culturing with Mouse Induced Pluripotent Stem Cells outside Body

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