The creation of the reconstruction of right ventricular outflow tract in rats_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

CHENG Yue ,  KANG Kai , CHUAI Junbo , QIN Xionghai , TIAN Xin , YANG Feng , JIANG Shulin , XIE Baodong

Department of Cardiovascular Surgery, 2nd Affiliated Hospital of Harbin Medical University, Key Laboratory of Myocardial Ischemia Mechanism and Treatment, Ministry of Education, Harbin, 150086, P.R.China;

Corresponding author：

KANG Kai, Email: kangkai1975@sina.com

Keywords：

Right ventricular outflow tract; reconstruction; rat; cardiac patch; tissue engineering

DOI：

10.7507/1007-4848.201811045

Video：

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Objective To investigate the feasibility of animal model of the reconstruction of right ventricular outflow tract in rats.Methods A total of 15 female Sprague-Dawley (SD) rats underwent right ventricular outflow tract reconstruction surgery. Before the operation, the collagen scaffolds were treated with g 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride chemistry (EDC), and seeded with human bone marrow stem cells (h-MSCs). Three days after the surgery, 3 rats were randomly sacrificed to evaluate the transmural resection of right ventricular outflow tract. One or 3 months later, other 3 rats at each timepoint were sacrificed, stained with Masson’s Trichrome to observe the degradation of scaffold. Furthermore, 4 weeks after the surgery, 4 rats were sacrificed and the hearts were sliced. Anti-human mitochondria staining was used to identify the survival of seeding cells.Results The transmural resection of right ventricular outflow tract was feasible in rats at an acceptable mortality (13.3%). After EDC treatment, the degradation rate of collagen scaffold was extended greatly. The seeding cells were detected by anti-mitochandria immunofluorescent staining in all patches 4 weeks after the operation.Conclusion Rat model of right ventricular outflow tract reconstruction could be a stable, reliable and economical screening model for engineered heart tissue research.

Citation： CHENG Yue, KANG Kai, CHUAI Junbo, QIN Xionghai, TIAN Xin, YANG Feng, JIANG Shulin, XIE Baodong. The creation of the reconstruction of right ventricular outflow tract in rats. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2019, 26(3): 260-263. doi: 10.7507/1007-4848.201811045 Copy

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6.	康凯, 曲辉, 汤继权, 等. 共价结合生长因子的胶原补片修补大鼠左心室室壁瘤的实验研究. 中华胸心血管外科杂志, 2012, 28(7): 429-432.
7.	Wu H, Li JZ, Xie BD, et al. Lower senescence of adipose-derived stem cells than donor-matched bone marrow stem cells for surgical ventricular restoration. Stem Cells Dev, 2018, 27(9): 612-623.
8.	Zhang C, Hou J, Zheng S, et al. Vascularized atrial tissue patch cardiomyoplasty with omentopexy improves cardiac performance after myocardial infarction. Ann Thorac Surg, 2011, 92(4): 1435-1442.
9.	Yuan Ye K, Sullivan KE, Black LD. Encapsulation of cardiomyocytes in a fibrin hydrogel for cardiac tissue engineering. J Vis Exp, 2011, (55): 3251.
10.	Wang, B, Borazjani A, Tahai M, et al. Fabrication of cardiac patch with decellularized porcine myocardial scaffold and bone marrow mononuclear cells. J Biomed Mater Res A, 2010, 94(4): 1100-1110.
11.	Ratcliffe MB, Wallace AW, Salahieh A, et al. Ventricular volume, chamber stiffness, and function after anteroapical aneurysm plication in the sheep. J Thorac Cardiovasc Surg, 2000, 119(1): 115-124.
12.	Cui J, Li J, Mathison M, et al. A clinically relevant large-animal model for evaluation of tissue-engineered cardiac surgical patch materials. Cardiovasc Revasc Med, 2005, 6(3): 113-120.
13.	Kang K, Sun L, Xiao Y, et al. Aged human cells rejuvenated by cytokine enhancement of biomaterials for surgical ventricular restoration. J Am Coll Cardiol, 2012, 60(21): 2237-2249.
14.	Mantakaki A, Fakoya AOJ, Sharifpanah F. Recent advances and challenges on application of tissue engineering for treatment of congenital heart disease. Peer J, 2018, 6: e5805.
15.	Chen J, Zhan Y, Wang Y, et al. Chitosan/silk fibroin modified nanofibrous patches with mesenchymal stem cells prevent heart remodeling post-myocardial infarction in rats. Acta Biomater, 2018, 80: 154-168.
16.	Yang JM, Olanrele OS, Zhang X, et al. Fabrication of hydrogel materials for biomedical applications. Adv Exp Med Biol, 2018, 1077: 197-224.

1. Bhagra CJ, Hickey EJ, Van De BruaeneA, et al. Pulmonary valve procedures late after repair of tetralogy of Fallot: current perpectives and contemporary approaches to management. Can J Cardiol, 2017, 33(9): 1138-1149.
2. Kalfa D, Bacha E. New technologies for surgery of the congenital cardiac defect. Rambam Maimonides Med J, 2013, 4(3): e0019.
3. Iop L, Palmosi T, Dal Sasso E, et al. Bioengineered tissue solutions for repair, correction and reconstruction in cardiovascular surgery. J Thorac Dis, 2018, 10(Suppl 20): S2390-S2411.
4. Carrabba M, Madeddu P. Current strategies for the manufacture of small size tissue engineering vascular grafts. Front Bioeng Biotechnol, 2018, 6: 41.
5. 康凯, 曲辉, 汤继权, 等. 共价结合生长因子的胶原补片改善大鼠室壁瘤修补术后移植细胞生存率的实验研究. 中国胸心血管外科临床杂志, 2013, 20(4): 451-456.
6. 康凯, 曲辉, 汤继权, 等. 共价结合生长因子的胶原补片修补大鼠左心室室壁瘤的实验研究. 中华胸心血管外科杂志, 2012, 28(7): 429-432.
7. Wu H, Li JZ, Xie BD, et al. Lower senescence of adipose-derived stem cells than donor-matched bone marrow stem cells for surgical ventricular restoration. Stem Cells Dev, 2018, 27(9): 612-623.
8. Zhang C, Hou J, Zheng S, et al. Vascularized atrial tissue patch cardiomyoplasty with omentopexy improves cardiac performance after myocardial infarction. Ann Thorac Surg, 2011, 92(4): 1435-1442.
9. Yuan Ye K, Sullivan KE, Black LD. Encapsulation of cardiomyocytes in a fibrin hydrogel for cardiac tissue engineering. J Vis Exp, 2011, (55): 3251.
10. Wang, B, Borazjani A, Tahai M, et al. Fabrication of cardiac patch with decellularized porcine myocardial scaffold and bone marrow mononuclear cells. J Biomed Mater Res A, 2010, 94(4): 1100-1110.
11. Ratcliffe MB, Wallace AW, Salahieh A, et al. Ventricular volume, chamber stiffness, and function after anteroapical aneurysm plication in the sheep. J Thorac Cardiovasc Surg, 2000, 119(1): 115-124.
12. Cui J, Li J, Mathison M, et al. A clinically relevant large-animal model for evaluation of tissue-engineered cardiac surgical patch materials. Cardiovasc Revasc Med, 2005, 6(3): 113-120.
13. Kang K, Sun L, Xiao Y, et al. Aged human cells rejuvenated by cytokine enhancement of biomaterials for surgical ventricular restoration. J Am Coll Cardiol, 2012, 60(21): 2237-2249.
14. Mantakaki A, Fakoya AOJ, Sharifpanah F. Recent advances and challenges on application of tissue engineering for treatment of congenital heart disease. Peer J, 2018, 6: e5805.
15. Chen J, Zhan Y, Wang Y, et al. Chitosan/silk fibroin modified nanofibrous patches with mesenchymal stem cells prevent heart remodeling post-myocardial infarction in rats. Acta Biomater, 2018, 80: 154-168.
16. Yang JM, Olanrele OS, Zhang X, et al. Fabrication of hydrogel materials for biomedical applications. Adv Exp Med Biol, 2018, 1077: 197-224.

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The creation of the reconstruction of right ventricular outflow tract in rats

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