• Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, 610041, P.R.China;
HU Jia, Email: humanjia@msn.com
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Objective To investigate the role and potential mechanisms of neuropilin-1 (NRP1) in the pathogenesis of vein graft failure.Methods The rat vascular smooth muscle cells (VSMCs) were transfected with NRP1-shRNA adenovirus and negative control adenovirus respectively. Cell counting kit-8, flow cytometry, Transwell and Western blot were used to investigate the effects of inhibition of NRP1 on VSMCs proliferation viability, apoptosis, migration capacity and its downstream signaling pathway protein expression.Results The proliferation and migration of rat VSMCs could be inhibited after down-regulation of NRP1, and the increase of apoptosis was also observed. Moreover, inhibition of NRP1 significantly reduced Akt and NF-κB phosphorylation in rat VSMCs, but had little effect on activation of ERK1/2.Conclusion NRP1 may promote vein graft hyperplastic remodeling by regulating the proliferation and migration of VSMCs through PI3K/Akt and NF-κB pathways, but further animal study is required.

Citation: LIU Yu, ZHANG Hongwei, YANG Peng, HUANG Yao, LU Chen, ZHANG Yu, HU Jia. Functional role and underlying mechanisms of neuropilin-1 in proliferation and migration of rat vascular smooth muscle cells. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2021, 28(7): 849-857. doi: 10.7507/1007-4848.202005091 Copy

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