Analysis of protein differences in aortic aneurysm/dissection based on tandem mass tag proteomics_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

ZHANG Yu , ZHANG Hongwei , YANG Peng , LU Chen , LIU Yu , WANG Haiyue ,  HU Jia

Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, 610041, P.R.China;

Corresponding author：

HU Jia, Email: humanjia@msn.com

Keywords：

Tandem mass tag proteomics; aortic aneurysm; aortic dissection; medial degeneration

DOI：

10.7507/1007-4848.202101075

Video：

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Objective To analyze the differences in proteins between aneurysm/dissection patients and healthy subjects, and subsequently figure out differential proteins related to medial degeneration of aortic aneurysm/dissection.Methods Aortic wall samples were collected from 6 male aortic aneurysm patients (an aortic aneurysm group, mean age 56.50±8.19 years), 6 male aortic dissection patients (an aortic dissection group, mean age 54.17±6.68 years) and 6 male healthy subjects (a normal group, mean age 40.50±9.31 years) between December 2019 and May 2020 in West China Hospital of Sichuan University. Quantitative proteomics was performed using tandem mass tag (TMT) techniques, followed by gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.Results A total of 63 differential proteins were obtained both in the aortic aneurysm group and the aortic dissection group compared with the normal group, with 30 up-regulating and 33 down-regulating. The differential proteins were involved in multiple biological processes and clusted on peroxisome proliferators-activated receptor (PPAR) signaling pathway, extracellular matrix-receptor interaction signaling pathway and complement and coagulation cascades signaling pathway.Conclusion The identified proteins may help to demonstrate new molecular mechanisms related to medial degeneration of aortic aneurysm/dissection.

Citation： ZHANG Yu, ZHANG Hongwei, YANG Peng, LU Chen, LIU Yu, WANG Haiyue, HU Jia. Analysis of protein differences in aortic aneurysm/dissection based on tandem mass tag proteomics. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2021, 28(10): 1222-1228. doi: 10.7507/1007-4848.202101075 Copy

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3.	中国心血管健康与疾病报告编写组. 中国心血管健康与疾病报告2019概要. 中国循环杂志, 2020, 35(9): 833-854.
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11.	Graham SH, Liu H. Life and death in the trash heap: The ubiquitin proteasome pathway and UCHL1 in brain aging, neurodegenerative disease and cerebral Ischemia. Ageing Res Rev, 2017, 34: 30-38.
12.	Takami Y, Nakagami H, Morishita R, et al. Ubiquitin carboxyl-terminal hydrolase L1, a novel deubiquitinating enzyme in the vasculature, attenuates NF-kappaB activation. Arterioscler Thromb Vasc Biol, 2007, 27(10): 2184-2190.
13.	Reinicke AT, Laban K, Sachs M, et al. Ubiquitin C-terminal hydrolase L1 (UCH-L1) loss causes neurodegeneration by altering protein turnover in the first postnatal weeks. Proc Natl Acad Sci U S A, 2019, 116(16): 7963-7972.
14.	Gao X, Wu L, Wang K, et al. Ubiquitin carboxyl terminal hydrolase L1 attenuates TNF-α-mediated vascular smooth muscle cell migration through suppression of NF-κB activation. Int Heart J, 2018, 59(6): 1409-1415.
15.	Cui GH, Shao SJ, Yang JJ, et al. Designer self-assemble peptides maximize the therapeutic benefits of neural stem cell transplantation for Alzheimer's disease via enhancing neuron differentiation and paracrine action. Mol Neurobiol, 2016, 53(2): 1108-1123.
16.	Chen ZL, Yao Y, Norris EH, et al. Ablation of astrocytic laminin impairs vascular smooth muscle cell function and leads to hemorrhagic stroke. J Cell Biol, 2013, 202(2): 381-395.
17.	Kleinhenz JM, Murphy TC, Pokutta-Paskaleva AP, et al. Smooth muscle-targeted overexpression of peroxisome proliferator activated receptor-γ disrupts vascular wall structure and function. PLoS One, 2015, 10(10): e0139756.
18.	Wang J, Zhang Q, Li S, et al. Low molecular weight fucoidan alleviates diabetic nephropathy by binding fibronectin and inhibiting ECM-receptor interaction in human renal mesangial cells. Int J Biol Macromol, 2020, 150: 304-314.
19.	Yan X, Li Y, Yu H, et al. Epigallocatechin-3-gallate inhibits H₂O₂-induced apoptosis in mouse vascular smooth muscle cells via 67 kD laminin receptor. Sci Rep, 2017, 7(1): 7774.
20.	Wasiak S, Gilham D, Tsujikawa LM, et al. Downregulation of the complement cascade in vitro, in mice and in patients with cardiovascular disease by the BET protein inhibitor apabetalone (RVX-208). J Cardiovasc Transl Res, 2017, 10(4): 337-347.
21.	Chen L, Fukuda N, Otsuki T, et al. Increased complement 3 with suppression of miR-145 induces the synthetic phenotype in vascular smooth muscle cells from spontaneously hypertensive rats. J Am Heart Assoc, 2019, 8(10): e012327.

1. Evangelista A, Isselbacher EM, Bossone E, et al. Insights from the International Registry of Acute Aortic Dissection: A 20-year experience of collaborative clinical research. Circulation, 2018, 137(17): 1846-1860.
2. Czerny M, Schmidli J, Adler S, et al. Current options and recommendations for the treatment of thoracic aortic pathologies involving the aortic arch: An expert consensus document of the european association for cardio-thoracic surgery (EACTS) and the european society for vascular surgery (ESVS). Eur J Cardiothorac Surg, 2019, 55(1): 133-162.
3. 中国心血管健康与疾病报告编写组. 中国心血管健康与疾病报告2019概要. 中国循环杂志, 2020, 35(9): 833-854.
4. Humphrey JD, Schwartz MA, Tellides G, et al. Role of mechanotransduction in vascular biology: Focus on thoracic aortic aneurysms and dissections. Circ Res, 2015, 116(8): 1448-1461.
5. Sherifova S, Holzapfel GA. Biomechanics of aortic wall failure with a focus on dissection and aneurysm: A review. Acta Biomater, 2019, 99: 1-17.
6. Saeyeldin AA, Velasquez CA, Mahmood SUB, et al. Thoracic aortic aneurysm: Unlocking the "silent killer" secrets. Gen Thorac Cardiovasc Surg, 2019, 67(1): 1-11.
7. Geyer PE, Holdt LM, Teupser D, et al. Revisiting biomarker discovery by plasma proteomics. Mol Syst Biol, 2017, 13(9): 942.
8. Takeda N, Hara H, Fujiwara T, et al. TGF-β signaling-related genes and thoracic aortic aneurysms and dissections. Int J Mol Sci, 2018, 19(7): 2125-2144.
9. Cifani N, Proietta M, Tritapepe L, et al. Stanford-A acute aortic dissection, inflammation, and metalloproteinases: A review. Ann Med, 2015, 47(6): 441-446.
10. Mevissen TET, Komander D. Mechanisms of deubiquitinase specificity and regulation. Annu Rev Biochem, 2017, 86: 159-192.
11. Graham SH, Liu H. Life and death in the trash heap: The ubiquitin proteasome pathway and UCHL1 in brain aging, neurodegenerative disease and cerebral Ischemia. Ageing Res Rev, 2017, 34: 30-38.
12. Takami Y, Nakagami H, Morishita R, et al. Ubiquitin carboxyl-terminal hydrolase L1, a novel deubiquitinating enzyme in the vasculature, attenuates NF-kappaB activation. Arterioscler Thromb Vasc Biol, 2007, 27(10): 2184-2190.
13. Reinicke AT, Laban K, Sachs M, et al. Ubiquitin C-terminal hydrolase L1 (UCH-L1) loss causes neurodegeneration by altering protein turnover in the first postnatal weeks. Proc Natl Acad Sci U S A, 2019, 116(16): 7963-7972.
14. Gao X, Wu L, Wang K, et al. Ubiquitin carboxyl terminal hydrolase L1 attenuates TNF-α-mediated vascular smooth muscle cell migration through suppression of NF-κB activation. Int Heart J, 2018, 59(6): 1409-1415.
15. Cui GH, Shao SJ, Yang JJ, et al. Designer self-assemble peptides maximize the therapeutic benefits of neural stem cell transplantation for Alzheimer's disease via enhancing neuron differentiation and paracrine action. Mol Neurobiol, 2016, 53(2): 1108-1123.
16. Chen ZL, Yao Y, Norris EH, et al. Ablation of astrocytic laminin impairs vascular smooth muscle cell function and leads to hemorrhagic stroke. J Cell Biol, 2013, 202(2): 381-395.
17. Kleinhenz JM, Murphy TC, Pokutta-Paskaleva AP, et al. Smooth muscle-targeted overexpression of peroxisome proliferator activated receptor-γ disrupts vascular wall structure and function. PLoS One, 2015, 10(10): e0139756.
18. Wang J, Zhang Q, Li S, et al. Low molecular weight fucoidan alleviates diabetic nephropathy by binding fibronectin and inhibiting ECM-receptor interaction in human renal mesangial cells. Int J Biol Macromol, 2020, 150: 304-314.
19. Yan X, Li Y, Yu H, et al. Epigallocatechin-3-gallate inhibits H₂O₂-induced apoptosis in mouse vascular smooth muscle cells via 67 kD laminin receptor. Sci Rep, 2017, 7(1): 7774.
20. Wasiak S, Gilham D, Tsujikawa LM, et al. Downregulation of the complement cascade in vitro, in mice and in patients with cardiovascular disease by the BET protein inhibitor apabetalone (RVX-208). J Cardiovasc Transl Res, 2017, 10(4): 337-347.
21. Chen L, Fukuda N, Otsuki T, et al. Increased complement 3 with suppression of miR-145 induces the synthetic phenotype in vascular smooth muscle cells from spontaneously hypertensive rats. J Am Heart Assoc, 2019, 8(10): e012327.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Analysis of protein differences in aortic aneurysm/dissection based on tandem mass tag proteomics

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