The relationship between miR-3187-5p and postoperative atrial fibrillation after coronary artery bypass grafting: A prospective cohort study_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

YAN Ziyi ¹ , ZHAO Haibo ² , HE Chunyue ² ,  LIU Yisi ¹

1. School of Nursing, Capital Medical University, Beijing, 100069, P. R. China;
2. Heart Center, Chao-Yang Hospital, Capital Medical University, Beijing, 100020, P. R. China;

Corresponding author：

LIU Yisi, Email: asceticsisi@aliyun.com

Keywords：

Postoperative atrial fibrillation; miR-3187-5p; coronary artery bypass grafting; bioinformatics; prospective cohort study

DOI：

10.7507/1007-4848.202206018

Video：

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Objective To investigate the relationship between miR-3187-5p in peripheral blood and pericardial drainage after coronary artery bypass grafting (CABG) and postoperative atrial fibrillation (POAF). Methods Patients who underwent CABG in the Heart Center of Beijing Chao-Yang Hospital from March to May 2022 were enrolled. Peripheral blood and pericardial drainage were collected at 0 h after surgery (immediate time for patients to return to ICU from operating room) to detect miR-3187-5p, and perioperative confounding factors were also collected. The miR-3187-5p was measured by quantitative real-time PCR and its regulated target genes were analyzed by bioinformatics. Results A total of 15 patients were enrolled, including 9 males and 6 females with an average age of 65.6±8.2 years. The incidence rate of POAF was 40.0%. miR-3187-5p in pericardial drainage at 0 h after surgery was an independent predictor for POAF. A total of 1 642 target genes of miR-3187-5p were predicted. GO function enrichment analysis and KEGG signal pathway enrichment analysis showed that target genes of miR-3187-5p were enriched in TGF-β, MAPK, Wnt and other classical collagen metabolic signal pathways, which might activate collagen metabolism by negatively regulating SMAD6 and other inhibitors of the pathways. Conclusion This study is the first to find that miR-3187-5p in pericardial drainage at 0 h after surgery is a potential, novel, and predictive factor for POAF, which may be related to the regulation of myocardial fibrosis signal pathways like TGF-β, MAPK and Wnt pathways, promoting the early collagen metabolism imbalance after CABG, increasing the collagen deposition in the atrium, and then promoting the early structural reconstruction after CABG and leading to the occurrence of POAF. The result provides a research basis for the accurate prediction and prevention of clinical POAF.

Citation： YAN Ziyi, ZHAO Haibo, HE Chunyue, LIU Yisi. The relationship between miR-3187-5p and postoperative atrial fibrillation after coronary artery bypass grafting: A prospective cohort study. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2023, 30(7): 1038-1047. doi: 10.7507/1007-4848.202206018 Copy

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2.	张凯, 王联群. 单纯非体外循环冠状动脉旁路移植术后新发房颤术前相关危险因素分析. 天津医药, 2018, 46(7): 708-711.
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13.	Gao F, Wang FG, Lyu RR, et al. SLC35E3 identified as a target of novel-m1061-5p via microRNA profiling of patients with cardiovascular disease. Mol Med Rep, 2018, 17(4): 5159-5167.
14.	中华医学会心血管病学分会介入心脏病学组, 中国医师协会心血管内科医师分会血栓防治专业委员会, 中华心血管病杂志编辑委员会. 中国经皮冠状动脉介入治疗指南(2016). 中华心血管病杂志, 2016, 44(5): 382-400.
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16.	Zhang ZJ, Song XG, Xie L, et al. Circulating serum exosomal miR-20b-5p and miR-3187-5p as efficient diagnostic biomarkers for early-stage non-small cell lung cancer. Exp Biol Med (Maywood), 2020, 245(16): 1428-1436.
17.	Zhang D, Cai X, Cai S, et al. Long intergenic non-protein coding RNA 01089 weakens tumor proliferation, migration, and invasion by sponging miR-3187-3p in non-small cell lung cancer. Cancer Manag Res, 2020, 12: 12151-12162.
18.	Zhang Z, Huang X, Yang J, et al. Identification and functional analysis of a three-miRNA ceRNA network in hypertrophic scars. J Transl Med, 2021, 19(1): 451.
19.	周羽靖. 体外循环下心脏瓣膜置换术后心包及纵膈引流液量变化规律的临床观察. 山西医科大学, 2019.
20.	李孟婷, 邓江, 杨丹莉, 等. 心肌肥厚过程中TGF-β和MAPK信号通路间的交互调节. 中国新药与临床杂志, 2022, 41(5): 257-263.
21.	陈智慧, 田海萍, 刘艳阳. 心肌纤维化相关通路的研究进展. 医学综述, 2022, 28(2): 259-264.
22.	余程. RNA乙酰化酶NAT10调控血管新生内膜形成的作用及机制研究. 福建医科大学, 2021.
23.	李雪. RRP12在胃癌发生中的作用及机制研究. 山东大学, 2020.
24.	边帅. GNL3L在食管鳞癌进展中的作用及机制的研究. 吉林大学, 2021.
25.	刘培军. RBM19、RBM23、RBM34和RBM47在肾透明细胞癌中的临床意义及对肿瘤发生发展的影响. 华中科技大学, 2017.
26.	魏峰. HEATR1基因与胃癌细胞增殖的相关性研究. 皖南医学院, 2018.
27.	阙冬冬, 宋旭东. 心房重构在房颤中的作用. 实用心电学杂志, 2021, 30(6): 404-407.
28.	Siontis GCM, Praz F, Lanz J, et al. New-onset arrhythmias following transcatheter aortic valve implantation: A systematic review and meta-analysis. Heart, 2018, 104(14): 1208-1215.
29.	Tao W, Wu F, Tang H, et al. Abstract 18260: Local interleukin-6 and pericardial drainage volume are associated with atrial fibrillation after coronary artery bypass grafting. Circulation, 2011, 21: A18260.
30.	Rader F, Pujara AC, Pattakos G, et al. Perioperative heart-type fatty acid binding protein levels in atrial fibrillation after cardiac surgery. Heart Rhythm, 2013, 10(2): 153-157.
31.	张泽生, 田荣成, 刘抗, 等. 冠状动脉旁路移植术后并发房颤的研究进展. 赣南医学院学报, 2019, 39(3): 283-287.

1. 王芯, 陶杰. 心脏直视手术术后房颤的研究进展. 山东医药, 2020, 60(13): 93-96.
2. 张凯, 王联群. 单纯非体外循环冠状动脉旁路移植术后新发房颤术前相关危险因素分析. 天津医药, 2018, 46(7): 708-711.
3. 张晓峰. OPCABG术前NLR、PLR与术后新发房颤的相关性研究. 山东大学, 2018.
4. 徐殊. RAAS、Apelin/APJ及MtDNA拷贝数与冠状动脉旁路移植术后新发房颤的相关性研究. 第四军医大学, 2017.
5. 孙殿珉. 心脏能量代谢障碍与CABG术后房颤易感性的研究. 北京协和医学院, 2015.
6. 赵乃时, 梅举. 冠状动脉搭桥术后早期新发房颤的临床研究进展. 中国心血管病研究, 2014, 12(2): 156-159.
7. 赵清, 杨文涛, 李向东, 等. MicroRNA与心房颤动心房纤维化关系的研究进展. 解放军医学院学报, 2021, 42(11): 1220-1224.
8. Feldman A, Moreira DAR, Gun C, et al. Analysis of circulating miR-1, miR-23a, and miR-26a in atrial fibrillation patients undergoing coronary bypass artery grafting surgery. Ann Hum Genet, 2017, 81(3): 99-105.
9. Tsoporis JN, Fazio A, Rizos IK, et al. Increased right atrial appendage apoptosis is associated with differential regulation of candidate microRNAs 1 and 133A in patients who developed atrial fibrillation after cardiac surgery. J Mol Cell Cardiol, 2018, 121: 25-32.
10. St-Onge S, Perrault LP, Demers P, et al. Pericardial blood as a trigger for postoperative atrial fibrillation after cardiac surgery. Ann Thorac Surg, 2018, 105(1): 321-328.
11. Xiao L, Liu C, Zhang S, et al. miR-3187-3p enhances migration and invasion by targeting PER2 in head and neck squamous cell carcinomas. J Cancer, 2021, 12(17): 5231-5240.
12. 苏晓明, 韩喆, 王洋, 等. 心脏术后心房颤动的研究进展. 中国医药, 2021, 16(11): 1731-1734.
13. Gao F, Wang FG, Lyu RR, et al. SLC35E3 identified as a target of novel-m1061-5p via microRNA profiling of patients with cardiovascular disease. Mol Med Rep, 2018, 17(4): 5159-5167.
14. 中华医学会心血管病学分会介入心脏病学组, 中国医师协会心血管内科医师分会血栓防治专业委员会, 中华心血管病杂志编辑委员会. 中国经皮冠状动脉介入治疗指南(2016). 中华心血管病杂志, 2016, 44(5): 382-400.
15. 王乃迪, 张海澄. 《2020ESC心房颤动诊断和管理指南》更新要点解读. 中国心血管病研究, 2020, 18(11): 966-973.
16. Zhang ZJ, Song XG, Xie L, et al. Circulating serum exosomal miR-20b-5p and miR-3187-5p as efficient diagnostic biomarkers for early-stage non-small cell lung cancer. Exp Biol Med (Maywood), 2020, 245(16): 1428-1436.
17. Zhang D, Cai X, Cai S, et al. Long intergenic non-protein coding RNA 01089 weakens tumor proliferation, migration, and invasion by sponging miR-3187-3p in non-small cell lung cancer. Cancer Manag Res, 2020, 12: 12151-12162.
18. Zhang Z, Huang X, Yang J, et al. Identification and functional analysis of a three-miRNA ceRNA network in hypertrophic scars. J Transl Med, 2021, 19(1): 451.
19. 周羽靖. 体外循环下心脏瓣膜置换术后心包及纵膈引流液量变化规律的临床观察. 山西医科大学, 2019.
20. 李孟婷, 邓江, 杨丹莉, 等. 心肌肥厚过程中TGF-β和MAPK信号通路间的交互调节. 中国新药与临床杂志, 2022, 41(5): 257-263.
21. 陈智慧, 田海萍, 刘艳阳. 心肌纤维化相关通路的研究进展. 医学综述, 2022, 28(2): 259-264.
22. 余程. RNA乙酰化酶NAT10调控血管新生内膜形成的作用及机制研究. 福建医科大学, 2021.
23. 李雪. RRP12在胃癌发生中的作用及机制研究. 山东大学, 2020.
24. 边帅. GNL3L在食管鳞癌进展中的作用及机制的研究. 吉林大学, 2021.
25. 刘培军. RBM19、RBM23、RBM34和RBM47在肾透明细胞癌中的临床意义及对肿瘤发生发展的影响. 华中科技大学, 2017.
26. 魏峰. HEATR1基因与胃癌细胞增殖的相关性研究. 皖南医学院, 2018.
27. 阙冬冬, 宋旭东. 心房重构在房颤中的作用. 实用心电学杂志, 2021, 30(6): 404-407.
28. Siontis GCM, Praz F, Lanz J, et al. New-onset arrhythmias following transcatheter aortic valve implantation: A systematic review and meta-analysis. Heart, 2018, 104(14): 1208-1215.
29. Tao W, Wu F, Tang H, et al. Abstract 18260: Local interleukin-6 and pericardial drainage volume are associated with atrial fibrillation after coronary artery bypass grafting. Circulation, 2011, 21: A18260.
30. Rader F, Pujara AC, Pattakos G, et al. Perioperative heart-type fatty acid binding protein levels in atrial fibrillation after cardiac surgery. Heart Rhythm, 2013, 10(2): 153-157.
31. 张泽生, 田荣成, 刘抗, 等. 冠状动脉旁路移植术后并发房颤的研究进展. 赣南医学院学报, 2019, 39(3): 283-287.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

The relationship between miR-3187-5p and postoperative atrial fibrillation after coronary artery bypass grafting: A prospective cohort study

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