Experimental Study of Relationship of Zonula Occludens-1 and Microvascular Injury in Rats with Severe Acute Pancreatitis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 JiangZhi-wei ,  WangTao , TangLi-jun , ChenGuang-yu , LiPeng-wu , ZhengXiao-bo , WangKe

Center of General Surgery, Chengdu General Hospital of Chengdu Military Command, Chengdu 610083, Sichuan Province, China;

Corresponding author：

WangTao, Email: watopo@163.com

Keywords：

Zona occludens-1; Severe acute pancreatitis; Tumor necrosis factor-α; Trypsin; Capillary barrier

DOI：

10.7507/1007-9424.20140267

Video：

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Objective To investigate the changes of expression of zonula occludens-1(ZO-1) in rats with severe acute pancreatitis (SAP), and to study the relationship between the ZO-1 protein and microvascular injury in rats with SAP. Methods Forty-eight Wistar rats were randomly divided into sham-operation (SO) group and SAP group, each group enrolled 24 rats. Pancreas of rats in SO group were flipped only after laparotomies, but rats of SAP group were injected with 5% sodium taurocholate by retrograding cholangiopancreatography micro pump to produce the SAP model. At 6, 12, and 24 hours after operation, 8 rats were sacrificed to get abdominal aortic blood for testing the levels of peripheral blood amylase, trypsin, interleukin-8(IL-8), tumor necrosis factor-α(TNF-α), and ZO-1 protein. At the same time, pancreatic tissues were got to perform HE staining and immunohistochemical staining for observation of the pathological changes and the expression of ZO-1 protein respectively. Results Compared with SO group at the same time, the levels of peripheral blood amylase, trypsin, IL-8, TNF-α, and ZO-1 protein were all higher in SAP group (P < 0.05). The level of amylase in SAP-24 hours group was higher than those of 6 hours group and 12 hours group(P < 0.05), the levels of trypsin, IL-8, and ZO-1 protein in SAP group increased over time (P < 0.05), but levels of TNF-αin 3 time points of SAP group did not differ with each other significantly(P > 0.05). Results of regression showed that in the SAP group, the level of ZO-1 protein in serum was significantly positive correlated with pathological score of pancreatic tissue(b=0.96, P < 0.05), levels of serum amylase(b=0.87, P < 0.05), trypsin(b=0.72, P < 0.05), and serum IL-8 (b=0.69, P < 0.05), but was not significantly correlated with level of TNF-α(P > 0.05). HE staining results showed that damage of pancreatic tissues became worse over time in SAP group, and the pathological score of SAP-6 hours group was lower than those of 12 hours group and 24 hours group (P < 0.05). Immunohistochemical staining results showed that, in SAP group, with the extension of time, the number of ZO-1 protein granules in pancreatic acinar cells and capillary wall reduced, and expressed in capillaries discontinuously. Conclusion During the course of SAP, the concentration of serum ZO-1 protein increase, but its expression in the pancreatic tissue degrade, which is closely associated with microvascular injury and progression of pancreatic tissues.

Citation： JiangZhi-wei, WangTao, TangLi-jun, ChenGuang-yu, LiPeng-wu, ZhengXiao-bo, WangKe. Experimental Study of Relationship of Zonula Occludens-1 and Microvascular Injury in Rats with Severe Acute Pancreatitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(9): 1114-1119. doi: 10.7507/1007-9424.20140267 Copy

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2.	Shaheen MA, Akhtar AJ. Organ failure associated with acute pancreatitis in African-American and Hispanic patients[J]. J Natl Med Assoc, 2007, 99(12):1402-1406.
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5.	Sawada N, Murata M, Kikuchi K, et al. Tight junctions and human diseases[J]. Med Electron Microsc, 2003, 36(3):147-156.
6.	Aho HJ, Koskensalo SM, Nevalainen TJ. Experimental pancreatitis in the rat. Sodium taurocholate-induced acute haemorrhagic pancreatitis[J]. Scand J Gastroenterol, 1980, 15(4):411-416.
7.	Schmidt J, Lewandrowsi K, Warshaw AL, et al. Morphometric characteristics and homogeneity of a new model of acute pancreatitis in the rat[J]. Int J Pancreatol, 1992, 12(1):41-51.
8.	Denker BM, Nigam SK. Molecular structure and assembly of the tight junction[J]. Am J Physiol, 1998, 274(1 Pt 2):F1-F9.
9.	Takeyama Y, Nishikawa J, Ueda T, et al. Involvement of peritoneal macrophage in the induction of cytotoxicity due to apoptosis in ascitic fluid associated with severe acute pancreatitis[J]. J Surg Res, 1999, 82(2):163-171.
10.	Zhou MT, Chen CS, Chen BC, et al. Acute lung injury and ARDS in acute pancreatitis:mechanisms and potential intervention[J]. World J Gastroenterol, 2010, 16(17):2094-2099.
11.	Kes P, Vucicević Z, Ratković-Gusić I, et al. Acute renal failure complicating severe acute pancreatitis[J]. Ren Fail, 1996, 18(4):621-628.
12.	Andrulli A, Perri F, Annese V. Guidelines for treatment of acute pancreatitis[J]. Gut, 1999, 44(4):579-580.
13.	Ljutić D, Piplović-Vuković T, Raos V, et al. Acute renal failure as a complication of acute pancreatitis[J]. Ren Fail, 1996, 18(4):629-633.
14.	Leppäniemi A, Johansson K, De Waele JJ. Abdominal compartment syndrome and acute pancreatitis[J]. Acta Clin Belg Suppl, 2007, (1):131-135.
15.	Katsuno T, Umeda K, Matsui T, et al. Deficiency of zonula occludens-1 causes embryonic lethal phenotype associated with defected yolk sac angiogenesis and apoptosis of embryonic cells[J]. Mol Biol Cell, 2008, 19(6):2465-2475.
16.	Blikslager AT, Moeser AJ, Gookin JL, et al. Restoration of barrier function in injured intestinal mucosa[J]. Physiol Rev, 2007, 87(2):545-564.
17.	彭波, 沙焕臣, 马清涌.一种新的胰腺炎脑损伤模型的建立[J].西安交通大学学报:医学版, 2010, 31(2):165-168.

1. Norman J. The role of cytokine in the pathogenesis of acute pancreatitis[J]. Am J Surg, 1998, 175(1):76-83.
2. Shaheen MA, Akhtar AJ. Organ failure associated with acute pancreatitis in African-American and Hispanic patients[J]. J Natl Med Assoc, 2007, 99(12):1402-1406.
3. Lytras D, Manes K, Triantopoulou C, et al. Persistent early organ failure:defining the high-risk group of patients with severe acute pancreatitis?[J]. Pancreas, 2008, 36(3):249-254.
4. Dobosz M, Mionskowska L, Hac S, et al. Heparin improves organ microcirculatory disturbances in caerulein-induced acute pancreatitis in rats[J]. World J Gastroenterol, 2004, 10(17):2553-2556.
5. Sawada N, Murata M, Kikuchi K, et al. Tight junctions and human diseases[J]. Med Electron Microsc, 2003, 36(3):147-156.
6. Aho HJ, Koskensalo SM, Nevalainen TJ. Experimental pancreatitis in the rat. Sodium taurocholate-induced acute haemorrhagic pancreatitis[J]. Scand J Gastroenterol, 1980, 15(4):411-416.
7. Schmidt J, Lewandrowsi K, Warshaw AL, et al. Morphometric characteristics and homogeneity of a new model of acute pancreatitis in the rat[J]. Int J Pancreatol, 1992, 12(1):41-51.
8. Denker BM, Nigam SK. Molecular structure and assembly of the tight junction[J]. Am J Physiol, 1998, 274(1 Pt 2):F1-F9.
9. Takeyama Y, Nishikawa J, Ueda T, et al. Involvement of peritoneal macrophage in the induction of cytotoxicity due to apoptosis in ascitic fluid associated with severe acute pancreatitis[J]. J Surg Res, 1999, 82(2):163-171.
10. Zhou MT, Chen CS, Chen BC, et al. Acute lung injury and ARDS in acute pancreatitis:mechanisms and potential intervention[J]. World J Gastroenterol, 2010, 16(17):2094-2099.
11. Kes P, Vucicević Z, Ratković-Gusić I, et al. Acute renal failure complicating severe acute pancreatitis[J]. Ren Fail, 1996, 18(4):621-628.
12. Andrulli A, Perri F, Annese V. Guidelines for treatment of acute pancreatitis[J]. Gut, 1999, 44(4):579-580.
13. Ljutić D, Piplović-Vuković T, Raos V, et al. Acute renal failure as a complication of acute pancreatitis[J]. Ren Fail, 1996, 18(4):629-633.
14. Leppäniemi A, Johansson K, De Waele JJ. Abdominal compartment syndrome and acute pancreatitis[J]. Acta Clin Belg Suppl, 2007, (1):131-135.
15. Katsuno T, Umeda K, Matsui T, et al. Deficiency of zonula occludens-1 causes embryonic lethal phenotype associated with defected yolk sac angiogenesis and apoptosis of embryonic cells[J]. Mol Biol Cell, 2008, 19(6):2465-2475.
16. Blikslager AT, Moeser AJ, Gookin JL, et al. Restoration of barrier function in injured intestinal mucosa[J]. Physiol Rev, 2007, 87(2):545-564.
17. 彭波, 沙焕臣, 马清涌.一种新的胰腺炎脑损伤模型的建立[J].西安交通大学学报:医学版, 2010, 31(2):165-168.

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Experimental Study of Relationship of Zonula Occludens-1 and Microvascular Injury in Rats with Severe Acute Pancreatitis

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