Experimental Study on The Cell Culture and Secretory Function of Human Parathyroid Cells_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 SUNHai-chen , LIUShuang , CUIYe-qing , WANGXiao-hui ,  LUOBin

Departmengt of General Surgery, Xuanwu Hospital of Capital Medical University, Beijing 100053, China;

Corresponding author：

LUOBin, Email: binluobj@sina.Com

Keywords：

Parathyroid cell; Cell culture; Cell transplantation; Parathyroid hormone

DOI：

10.7507/1007-9424.20140269

Video：

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Objective To investigate the way to culture human parathyroid cells and to investigate its secretory function. Methods After digested by collagenase, parathyroid cells were isolated to get the original generation cells, then the cells were cultured and passaged, and morphological changes of original generation cells and passage cells were observed on every day. The parathyroid hormone(PTH) level secreted by the original generation cells and passage cells were measured on the 1^st, 5^th, 10^th, 15^th, and 20^th day(original generation cells only) respectively. Results The cellular morphology was complete after digestion. On the 2^nd day, most of the parathyroid cells had adhered and spreaded, on the 3^rd day, all cells had spread. There was no very obvious changes on these cells after cultured for 4-15 days. From 16 to 20 days, some parathyroid cells went senescence. On the 1^st day, all of the passage cells, which were fusiform and little bigger than those of the original generation cells, had adhered and spreaded. From 2 to 15 days, there was no very obvious changes. The concentration of PTH in original generation cells begin to decreased significantly on the 10^th day (P < 0.01). The concentration of PTH in passage cells were all lower than those of original generation cells at the same corresponding time, but there were no significant difference on the PTH level on 5^th day and 1^st day, 10^th day and 5^th day, 15^th day and 10^th day in passage cells (P > 0.05). Conclusion Parathyroid cells which were cultured within 10 days possess well morphologic structure and have the strongest secretory function. Although the passage cells still possess secretory function, it is greatly inferior to original generation cells. At last, we consider that original generation cells cultured within 10 days can be regarded as the source of allogeneic cell transplantation.

Citation： SUNHai-chen, LIUShuang, CUIYe-qing, WANGXiao-hui, LUOBin. Experimental Study on The Cell Culture and Secretory Function of Human Parathyroid Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(9): 1120-1123. doi: 10.7507/1007-9424.20140269 Copy

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2.	鄂征. 组织培养和分子细胞学技术[M]. 北京:北京出版社, 1994:87-89.
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12.	宋纯, 宋一民, 武林枫, 等. 人胚甲状旁腺细胞移植治疗甲状旁腺功能低下症[J]. 中华外科杂志, 2000, 38(9):690-692.
13.	段秀庆, 宋纯, 王江滨, 等. 大鼠甲状旁腺细胞经培养后再移植的实验研究[J]. 中国普外基础与临床杂志, 2004, 11(6):486-488.
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19.	段秀庆, 李永宁, 宋纯. 大鼠甲状旁腺细胞体外培养及功能的实验研究[J]. 中国现代普通外科进展, 2008, 11(5):389-391.
20.	Picariello L, Benvenuti S, Recenti R, et al. Microencapsulation of human parathyroid cells:an "in vitro" study[J]. J Surg Res, 2001, 96(1):81-89.
21.	唐功耀, 张凌. 微囊免疫隔离技术在医学中的应用[J]. 临床药物治疗杂志, 2012, 10(2):27-30, 40.
22.	杨晓昀, 陈儿同, 周燕, 等. 微囊化技术在医学领域中的应用与进展[J]. 中国组织工程研究与临床康复, 2010, 14(29):5483-5488.
23.	刘春蓉, 苗军, 余鹏, 等. 微囊化人工细胞技术及其应用[J]. 中国组织工程研究与临床康复, 2007, 11(5):949-952.
24.	沈阳, 汤亭亭. 细胞微囊技术在再生医学领域的应用[J]. 国际骨科学杂志, 2011, 32(2):81-82, 86.

1. 黄家驷. 外科学[M]. 北京:人民卫生出版社, 1994:887.
2. 鄂征. 组织培养和分子细胞学技术[M]. 北京:北京出版社, 1994:87-89.
3. 马冬冬, 季冠芳, 徐志惠, 等. 甲状旁腺激素PTH (1-34)的二级结构及其功能研究[J]. 天津医科大学学报, 2002, 8(3):314-315, 373.
4. 李成, 张伟辉. 甲状旁腺功能低下症治疗现状[J]. 哈尔滨医科大学学报, 2004, 38(4):405-407.
5. 高恒元, 周冬仙, 刘新杰, 等. Ⅰ期甲状旁腺自体移植的临床研究进展[J]. 中国医师进修杂志, 2010, 33(8):73-74.
6. 涂刚, 罗浩军, 姚榛祥. 甲状旁腺移植现状[J]. 内分泌外科杂志, 2008, 2(5):331-334, 351.
7. 曹利平, 汪亮. 甲状旁腺移植现状及趋势[J]. 中国实用外科杂志, 2008, 28(3):227-229.
8. 高庆军, 赵代伟, 王南鹏, 等. 同种异体甲状旁腺组织移植治疗甲状旁腺功能低下的实验研究[J]. 中国普外基础与临床杂志, 2012, 19(10):1079-1084.
9. Schachter P, Christy MD, Leight GS, et al. Function in athymic nude mice of parathyroid heterografts from patients with primary hyperparathyroidism and secondary hyperparathyroidism[J]. Surgery, 1990, 108(6):1040-1046.
10. Kadowaki MH, Fulton N, Schark C, et al. Difficulties of parathyroidectomy after previous thyroidectomy[J]. Surgery, 1989, 106(6):1018-1024.
11. Tsuji K, Fuchinoue S, Kai K, et al. Culture of human parathyroid cells for transplantation[J]. Transplant Proc, 1999, 31(7):2697.
12. 宋纯, 宋一民, 武林枫, 等. 人胚甲状旁腺细胞移植治疗甲状旁腺功能低下症[J]. 中华外科杂志, 2000, 38(9):690-692.
13. 段秀庆, 宋纯, 王江滨, 等. 大鼠甲状旁腺细胞经培养后再移植的实验研究[J]. 中国普外基础与临床杂志, 2004, 11(6):486-488.
14. 马凤贤, 王江滨, 张亚仙. 甲状旁腺功能低下患者细胞移植及其护理[J]. 护理学杂志, 2005, 20(14):42-43.
15. 杨冰, 王梅. 高磷对大鼠甲状旁腺组织及原代培养细胞的刺激作用[J]. 中国血液净化, 2008, 7(6):317-320.
16. 宋纯, 张伟辉, 宋春芳, 等. 体外培养甲状旁腺细胞形态与功能的实验研究[J]. 局解手术学杂志, 1997, 6(4):42-44.
17. 宋一民, 周毅, 武林枫, 等. 人胚甲状旁腺细胞移植供体胎龄的选择[J]. 伤残医学杂志, 2001, 9(1):4-6.
18. 邰升, 宋一民, 宋纯, 等. 人胎甲状旁腺细胞培养形态与功能的研究[J]. 哈尔滨医科大学学报, 2000, 34(6):409-411.
19. 段秀庆, 李永宁, 宋纯. 大鼠甲状旁腺细胞体外培养及功能的实验研究[J]. 中国现代普通外科进展, 2008, 11(5):389-391.
20. Picariello L, Benvenuti S, Recenti R, et al. Microencapsulation of human parathyroid cells:an "in vitro" study[J]. J Surg Res, 2001, 96(1):81-89.
21. 唐功耀, 张凌. 微囊免疫隔离技术在医学中的应用[J]. 临床药物治疗杂志, 2012, 10(2):27-30, 40.
22. 杨晓昀, 陈儿同, 周燕, 等. 微囊化技术在医学领域中的应用与进展[J]. 中国组织工程研究与临床康复, 2010, 14(29):5483-5488.
23. 刘春蓉, 苗军, 余鹏, 等. 微囊化人工细胞技术及其应用[J]. 中国组织工程研究与临床康复, 2007, 11(5):949-952.
24. 沈阳, 汤亭亭. 细胞微囊技术在再生医学领域的应用[J]. 国际骨科学杂志, 2011, 32(2):81-82, 86.

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Experimental Study on The Cell Culture and Secretory Function of Human Parathyroid Cells

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