Inhibition Role ofγ-Ray on Echinococcus Granulosus Protoscoleces in Mice <i>In Vivo</i>_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 YUANQing ¹ ,  LIBo ² , JIANGShi-ping ¹ , LUOLan-lun ³ , LIGeng ¹ , ZHAOQiang ¹ , ZHANGLi-yong ¹

1. Department of General Surgery, People's Hospital of Yaan City, Yaan 625000, Sichuan Province, China;
2. Department of Hepatobiliary Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China;
3. Department of Hepatobiliary Surgery, Sichuan Provincial People's Hospital, Chengdu 610072, Sichuan Province, China;

Corresponding author：

LIBo, Email: liboer2002@126.com

Keywords：

Gamma ray; Echinococcosis granulosus protoscoleces; Radiotherapy; Inhibition of growth

DOI：

10.7507/1007-9424.20150039

Video：

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Objective To investigate the effect ofγ-ray irradiation on echinococcus protoscoleces before abdominal cavity implantation in mice. Methods Echinococcus protoscoleces were cultured in vitro, and then divided into 5 groups, four groups receivedγ-ray irradiation with 10 Gy, 20 Gy, 40 Gy, and 80 Gy, respectively. The remaining control group was not irradiated. The protoscoleces were then implanted into the abdominal cavity of mice. Four months later, the incidence of echinococcosis was measured by detection of echinococcus antibody in the blood and then abdominal laparotomy was performed to isolate intraabdominal cysts, and the weight and structure of the echinococcus generated from the protoscoleces were measured and observed. Results After implantation of irradiated echinococcus protoscoleces in mice, the incidence of echinococcosis was 100% in the control group, 80.0% in the 10 Gy group, 33.3% in the 20 Gy group, 33.3% in the 40 Gy group, and 26.7% in the 80 Gy group. The echinococci had lower weight in the irradiated groups with 10 Gy (35.80 mg), with 20 Gy (0.00 mg), with 40 Gy (0.00 mg), and with 80 Gy (0.00 mg) as compared with the control group (157.80 mg). The echinococcus generated from the protoscoleces appeared calcification. Conclusions γ-ray irradiation could inhibit the formation of echinococcus granulosus generated from protoscoleces, damage the structure of echinococcus generated from the protoscoleces. Irradiation might prevent echinococcosis recurrence after surgical removal of hydatid cysts.

Citation： YUANQing, LIBo, JIANGShi-ping, LUOLan-lun, LIGeng, ZHAOQiang, ZHANGLi-yong. Inhibition Role ofγ-Ray on Echinococcus Granulosus Protoscoleces in Mice In Vivo. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(2): 139-143. doi: 10.7507/1007-9424.20150039 Copy

1.	包根书, 张虹, 景涛, 等.阿苯达唑亚砜及其对映体体外抗细粒棘球绦虫原头蚴作用.中国寄生虫学与寄生虫病杂志, 2008, 26(6): 459-461, 465.
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3.	吕海龙, 彭心宇, 张士杰, 等.阿苯达唑脂质体及槐耳浸膏对小鼠肝细粒棘球蚴感染率影响的实验观察.中国寄生虫学与寄生虫病杂志, 2008, 26(5): 361-365.
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6.	吕西, 李徐生.腹腔镜治疗肝包虫的现状与展望.中华腔镜外科杂志:电子版, 2010, 3(1): 75-79.
7.	Gollackner B, Längle F, Auer H, et al. Radical surgical therapy of abdominal cystic hydatid disease: factors of recurrence. World J Surg, 2000, 24(6): 717-721.
8.	Uhl W, Loftier H, Zimmer MA, et al. Surgical treatment of echinococcosis of the liver. Swiss Surg, 1999, 5(1): 126-132.
9.	袁庆, 李波, 贺凯, 等.伽马射线体外照射对细粒棘球蚴原头节的杀灭作用.中国人兽共患病学报, 2013, 29(9): 904-908.
10.	赵春芳, 温浩, 王建华, 等. 3种药物体外抗细粒棘球蚴原头节作用的比较.中国寄生虫病防治杂志, 2002, 15(1): 35-38.
11.	袁丽英, 张壮志, 石保新, 等.细粒棘球蚴-原头蚴体外培养成囊模型的建立.畜牧与兽医, 2009, 41(7): 29-31.
12.	温浩, 丁兆勋.包虫病图谱第二版.北京:科学出版社, 2008: 28-29.
13.	曹得萍, 马祁生, 赵海龙.藏药治疗小鼠继发性棘球蚴超微结构的观察.中国人兽共患病学报, 2006, 22(7): 654-656.
14.	Smyth TD, Heath DD. Pathogeresis of larval cestodes in mammals. Helminthol Abs, 1970, 39: 1-23.
15.	张积仁, 刘端祺.肿瘤物理治疗新技术.北京:人民军医出版社, 2005: 224-225.

1. 包根书, 张虹, 景涛, 等.阿苯达唑亚砜及其对映体体外抗细粒棘球绦虫原头蚴作用.中国寄生虫学与寄生虫病杂志, 2008, 26(6): 459-461, 465.
2. 康金凤, 胡汉华, 白山别克, 等.过氧化氢体外诱导细粒棘球蚴原头节细胞凋亡的实验观察.中国寄生虫学与寄生虫病杂志, 2008, 26(5): 332-337.
3. 吕海龙, 彭心宇, 张士杰, 等.阿苯达唑脂质体及槐耳浸膏对小鼠肝细粒棘球蚴感染率影响的实验观察.中国寄生虫学与寄生虫病杂志, 2008, 26(5): 361-365.
4. Adas G, Arikan S, Kemik O, et al. Use of albendazole sulfoxide, albendazole sulfone, and combined solutions as scolicidal agents on hydatid cysts (in vitro study). World J Gastroenterol, 2009, 15(1): 112-116.
5. 蒋松, 贾斌, 陈玲香, 等.阿苯达唑及其脂质体治疗羊肝包虫病的病理学比较观察.黑龙江畜牧兽医:科技版, 2009, 10(10): 82-83.
6. 吕西, 李徐生.腹腔镜治疗肝包虫的现状与展望.中华腔镜外科杂志:电子版, 2010, 3(1): 75-79.
7. Gollackner B, Längle F, Auer H, et al. Radical surgical therapy of abdominal cystic hydatid disease: factors of recurrence. World J Surg, 2000, 24(6): 717-721.
8. Uhl W, Loftier H, Zimmer MA, et al. Surgical treatment of echinococcosis of the liver. Swiss Surg, 1999, 5(1): 126-132.
9. 袁庆, 李波, 贺凯, 等.伽马射线体外照射对细粒棘球蚴原头节的杀灭作用.中国人兽共患病学报, 2013, 29(9): 904-908.
10. 赵春芳, 温浩, 王建华, 等. 3种药物体外抗细粒棘球蚴原头节作用的比较.中国寄生虫病防治杂志, 2002, 15(1): 35-38.
11. 袁丽英, 张壮志, 石保新, 等.细粒棘球蚴-原头蚴体外培养成囊模型的建立.畜牧与兽医, 2009, 41(7): 29-31.
12. 温浩, 丁兆勋.包虫病图谱第二版.北京:科学出版社, 2008: 28-29.
13. 曹得萍, 马祁生, 赵海龙.藏药治疗小鼠继发性棘球蚴超微结构的观察.中国人兽共患病学报, 2006, 22(7): 654-656.
14. Smyth TD, Heath DD. Pathogeresis of larval cestodes in mammals. Helminthol Abs, 1970, 39: 1-23.
15. 张积仁, 刘端祺.肿瘤物理治疗新技术.北京:人民军医出版社, 2005: 224-225.

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Inhibition Role ofγ-Ray on Echinococcus Granulosus Protoscoleces in Mice In Vivo

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