Study The Expressions of FN and PTEN in Hepatocellular Carcinoma and Its Clinical Significance_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 WUJin-zhu ,  CAIWei-hua , GUChun-yan , LURen-fei , WUJian-jun , XIAOXu , WANGJian-xin , ZHURen-fei

Department of Hepatobiliary Surgery, The Third Affiliated Hospital, Nantong University, Nantong 226010, Jiangsu Province, China;

Corresponding author：

CAIWei-hua, Email: 39235978@qq.com

Keywords：

Fibronectin; Phosphatase and tensin homology deleted on chromosome ten; Hepatocellular carcinoma; Gene expression

DOI：

10.7507/1007-9424.20150212

Video：

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Objective To explore the relationship between the expressions of fibronectin (FN) and phosphatase and tensin homology deleted on ehromosome ten (PTEN) in hepatocellular carcinoma (HCC) tissues and the clinical pathological features. Methods The expressions of FN and PTEN were detected by using Western blot and immunohistochemistry respectively in 83 HCC tissues and para-carcinoma tissues, 47 hepatic cirrhosis tissues and 11 normal hepatic tissues. The correlations between the expressions of FN and PTEN and the clinicopathologic features of HCC patients were analyzed. Results The positive expression rate of FN protein in HCC tissues was significantly higher than those in para-carcinoma tissue, normal hepatic tissue, and liver cirrhosis tissues (P<0.05); meanwhile the expression of PTEN was opposite (P<0.05). The positive expression rate of FN protein in para-carcinoma tissues was also obviously higher than that liver cirrhosis tissues and normal hepatic tissues (P<0.05), meanwhile the expression of PTEN was opposite (P<0.05). The positive expression rate of FN protein was higher in HCC tissues with cancer embolus, lymphatic metastasis, positive AFP, and multiple tumor (P<0.05), but there were no statistically significant differences in FN protein expression, gender, age, HBsAg, degree of tumor differentiation, and size of tumor (P>0.05). The positive expression rate of PTEN was lower in HCC tissues with high-medium differentiation, cancer embolus, positive AFP, lymphatic metastasis, and tumor diameter ≥2 cm (P<0.05), there were no statistically significant differences in PTEN expression, gender, age, HBsAg, and the number of tumor (P>0.05). Conclusions The abnormal expressions of FN and PTEN in HCC tissues which may play a role in promoting or inhibiting occurrence, development, invasion, and metastasis of HCC. The abnormal expressions of both can be used as molecular biological markers for the malignant degree, invasion, and metastasis of HCC.

Citation： WUJin-zhu, CAIWei-hua, GUChun-yan, LURen-fei, WUJian-jun, XIAOXu, WANGJian-xin, ZHURen-fei. Study The Expressions of FN and PTEN in Hepatocellular Carcinoma and Its Clinical Significance. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(7): 822-827. doi: 10.7507/1007-9424.20150212 Copy

1.	Silveira AP, Da Silva Manoel-Caetano F, Aoki S, et al. Gene mutations and polymorphisms of TP53 and FHIT in chronic esophagitis and esophageal carcinoma. Anticancer Res, 2011, 31(5):1685-1690.
2.	Pollicino T, Saitta C, Raimondo G. Hepatocellular carcinoma:the point of view of the hepatitis B virus. Carcinogenesis, 2011, 32(8):1122-1132.
3.	康凯夫, 张鑫, 陈小伍, 等. MMP-7和PTEN蛋白在原发性肝细胞性肝癌中的表达及其意义. 肿瘤防治研究, 2010, 37(6):652-655.
4.	刘梅, 杨胜利, 杨付叶, 等. 一个新的与纤粘连蛋白相互作用促进肝癌细胞迁移的基因. 中国肿瘤临床, 2006, 33 (14):781-783.
5.	Obara M, Sakuma T, Fujikawa K. The third type Ⅲ module of human fibroectin mediates cell adhesion and migration. J Biochem, 2010, 147(3):327-335.
6.	Antonyak MA, Li B, Boroughs JL, et al. Cancer cell-derived microvesicles induce transformation by transferring tissue tran sglutaminase and fibronectin to recipient cues. Proc Natl Acad Sci USA, 2011, 108(12):4852-4857.
7.	王丽. 抑癌基因PTEN在肝癌组织中的表达及HCV核心蛋白对PTEN作用的研. 西安:第四军医大学, 2007.
8.	秦权林. PTEN基因多态性及PTEN蛋白表达与家族聚集性肝癌遗传易感性研究. 南宁:广西医科大学, 2009.
9.	Liu H, Huang X, Liu X, et al. miR-21 promotes human nucleus pulposus cell proliferation through PTEN/AKT signaling. Int J Mol Sci, 2014, 15(3):4007-4018.
10.	Athnassiadou P, Athanassiadesy P, Grapsa D, et al. The prognostic value of PTEN, p53, and beta-catenin in endometrial carcinoma:a prospective immunocytochemical study. Int J Gynecol Cancer, 2007, 17(3):697-704.
11.	Kechagioglou P, Papi RM, Provatopoulou X, et al. Tumor suppressor PTEN in breast cancer:heterozygosity, mutations and protein expression. Anticancer Res, 2014, 34(3):1387-1400.
12.	Stanich PP, Pilarski R, Rock J, et al. Colonic manifestations of PTEN hamartoma tumor syndrome:case series and systematic review. World J Gastroenterol, 2014, 20(7):1833-1838.
13.	Nogueira C, Kim KH, Sung H, et al. Cooperative interactions of PTEN deficiency and RAS activation in melanoma metastasis. Oncogene, 2010, 29(47):6222-6232.
14.	张缨, 贾绍昌, 项方, 等. miR-21在肝癌细胞中的表达及其与抑癌基因PTEN的关系. 临床肿瘤学杂志, 2014, 19(6):490-493.
15.	Zhang M, Shan BE, Yuan NF, et al. Effect of topotecan on retinocytoma cell apoptosis and expression of Livin and PTEN. Chin Med J (Engl), 2013, 126(2):340-344.
16.	白丽, 任锋, 郑素军, 等. 抑制PTEN对小鼠肝缺血再灌注损伤的保护作用及其机制. 中华肝脏病杂志, 2014, 22(6):451-455.
17.	郝礼森, 张晓岚, 任昌镇, 等. PTEN过表达抑制体外活化肝星状细胞Akt的磷酸化. 实用医学杂志, 2014, 30(7):1069-1072.
18.	张蕾, 杨芳, 张晓敏, 等. HBsAg阳性肝癌PTEN基因表达的研究. 滨州医学院学报. 2014, 37(4):241-243.
19.	张鸣杰, 张国雷, 魏云海, 等. FHIT、FN和PTEN在肝癌组织中的表达及临床意义. 中华普通外科杂志, 2012, 27(6):487-490.
20.	李秋阳, 陈惠丽, 李宁, 等. MMP-9和PTEN蛋白在胶质瘤中的表达特点及意义. 中国实验诊断学, 2013, 17(10):1811-1814.
21.	Chen F, Yang D, Wang S, et al. Livin regulates prostate cancer cell invasion by impacting the NF-kβsignaling pathway and the expression of FN and CXCR4. IUBMB Life, 2012, 64(3):274-283.
22.	Zhang Z, Pan J, Li L, et al. Survey of risk factors contributed to lymphatic metastasis in patients with oral tongue squamous cell carcinoma by immunohistochemistry. J Oral Pathol Med, 2011, 40(2):127-134.
23.	黄宗明, 余英豪, 于媛媛, 等. 胃癌组织中CD44v6、VEGF、PTEN的表达与临床意义. 临床误诊误治杂志, 2012, 25(3):39-42.
24.	王鹏, 郭琳, 王强. PTEN、Fas/FasL和MMP-2在胃癌组织的表达及其临床意义. 中国普外基础与临床杂志, 2009, 16(12):991-995.
25.	丛玲华, 高志安, 钟国平, 等. 人肺癌组织E-cadherin和PTEN及HIF-1α蛋白表达临床意义的探讨. 中华肿瘤防治杂志, 2013, 20(3):196-199.
26.	钟育波, 孙凯, 张晓槟, 等. X线辐射剂量对结直肠癌细胞microRNA-221和PTEN表达的影响. 中国普外基础与临床杂志, 2013, 20(10):1113-1116.

1. Silveira AP, Da Silva Manoel-Caetano F, Aoki S, et al. Gene mutations and polymorphisms of TP53 and FHIT in chronic esophagitis and esophageal carcinoma. Anticancer Res, 2011, 31(5):1685-1690.
2. Pollicino T, Saitta C, Raimondo G. Hepatocellular carcinoma:the point of view of the hepatitis B virus. Carcinogenesis, 2011, 32(8):1122-1132.
3. 康凯夫, 张鑫, 陈小伍, 等. MMP-7和PTEN蛋白在原发性肝细胞性肝癌中的表达及其意义. 肿瘤防治研究, 2010, 37(6):652-655.
4. 刘梅, 杨胜利, 杨付叶, 等. 一个新的与纤粘连蛋白相互作用促进肝癌细胞迁移的基因. 中国肿瘤临床, 2006, 33 (14):781-783.
5. Obara M, Sakuma T, Fujikawa K. The third type Ⅲ module of human fibroectin mediates cell adhesion and migration. J Biochem, 2010, 147(3):327-335.
6. Antonyak MA, Li B, Boroughs JL, et al. Cancer cell-derived microvesicles induce transformation by transferring tissue tran sglutaminase and fibronectin to recipient cues. Proc Natl Acad Sci USA, 2011, 108(12):4852-4857.
7. 王丽. 抑癌基因PTEN在肝癌组织中的表达及HCV核心蛋白对PTEN作用的研. 西安:第四军医大学, 2007.
8. 秦权林. PTEN基因多态性及PTEN蛋白表达与家族聚集性肝癌遗传易感性研究. 南宁:广西医科大学, 2009.
9. Liu H, Huang X, Liu X, et al. miR-21 promotes human nucleus pulposus cell proliferation through PTEN/AKT signaling. Int J Mol Sci, 2014, 15(3):4007-4018.
10. Athnassiadou P, Athanassiadesy P, Grapsa D, et al. The prognostic value of PTEN, p53, and beta-catenin in endometrial carcinoma:a prospective immunocytochemical study. Int J Gynecol Cancer, 2007, 17(3):697-704.
11. Kechagioglou P, Papi RM, Provatopoulou X, et al. Tumor suppressor PTEN in breast cancer:heterozygosity, mutations and protein expression. Anticancer Res, 2014, 34(3):1387-1400.
12. Stanich PP, Pilarski R, Rock J, et al. Colonic manifestations of PTEN hamartoma tumor syndrome:case series and systematic review. World J Gastroenterol, 2014, 20(7):1833-1838.
13. Nogueira C, Kim KH, Sung H, et al. Cooperative interactions of PTEN deficiency and RAS activation in melanoma metastasis. Oncogene, 2010, 29(47):6222-6232.
14. 张缨, 贾绍昌, 项方, 等. miR-21在肝癌细胞中的表达及其与抑癌基因PTEN的关系. 临床肿瘤学杂志, 2014, 19(6):490-493.
15. Zhang M, Shan BE, Yuan NF, et al. Effect of topotecan on retinocytoma cell apoptosis and expression of Livin and PTEN. Chin Med J (Engl), 2013, 126(2):340-344.
16. 白丽, 任锋, 郑素军, 等. 抑制PTEN对小鼠肝缺血再灌注损伤的保护作用及其机制. 中华肝脏病杂志, 2014, 22(6):451-455.
17. 郝礼森, 张晓岚, 任昌镇, 等. PTEN过表达抑制体外活化肝星状细胞Akt的磷酸化. 实用医学杂志, 2014, 30(7):1069-1072.
18. 张蕾, 杨芳, 张晓敏, 等. HBsAg阳性肝癌PTEN基因表达的研究. 滨州医学院学报. 2014, 37(4):241-243.
19. 张鸣杰, 张国雷, 魏云海, 等. FHIT、FN和PTEN在肝癌组织中的表达及临床意义. 中华普通外科杂志, 2012, 27(6):487-490.
20. 李秋阳, 陈惠丽, 李宁, 等. MMP-9和PTEN蛋白在胶质瘤中的表达特点及意义. 中国实验诊断学, 2013, 17(10):1811-1814.
21. Chen F, Yang D, Wang S, et al. Livin regulates prostate cancer cell invasion by impacting the NF-kβsignaling pathway and the expression of FN and CXCR4. IUBMB Life, 2012, 64(3):274-283.
22. Zhang Z, Pan J, Li L, et al. Survey of risk factors contributed to lymphatic metastasis in patients with oral tongue squamous cell carcinoma by immunohistochemistry. J Oral Pathol Med, 2011, 40(2):127-134.
23. 黄宗明, 余英豪, 于媛媛, 等. 胃癌组织中CD44v6、VEGF、PTEN的表达与临床意义. 临床误诊误治杂志, 2012, 25(3):39-42.
24. 王鹏, 郭琳, 王强. PTEN、Fas/FasL和MMP-2在胃癌组织的表达及其临床意义. 中国普外基础与临床杂志, 2009, 16(12):991-995.
25. 丛玲华, 高志安, 钟国平, 等. 人肺癌组织E-cadherin和PTEN及HIF-1α蛋白表达临床意义的探讨. 中华肿瘤防治杂志, 2013, 20(3):196-199.
26. 钟育波, 孙凯, 张晓槟, 等. X线辐射剂量对结直肠癌细胞microRNA-221和PTEN表达的影响. 中国普外基础与临床杂志, 2013, 20(10):1113-1116.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Study The Expressions of FN and PTEN in Hepatocellular Carcinoma and Its Clinical Significance

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