Expression of NGAL in Pancreatic Cancer Tissues_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 LIUYan-sen , YUJing-xi , ZENGHong-wen

Department of General Surgery, The Third People's Hospital of Huizhou City, Huizhou 516000, Guangdong Province, China;

Corresponding author：

LIUYan-sen, Email: eroico@163.com

Keywords：

Pancreatic cancer; Neutrophil gelatinase associated lipocalin; Gene expression; Pprotein expression; Nude mice

DOI：

10.7507/1007-9424.20160008

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To study the expression of lipid associated with neutrophil gelatinase associated lipocalin (NGAL) in nude mice orthotopic pancreatic cancer tissues and the relationship between the occurred and development of pancreatic cancer. Methods The expressions of NGAL mRNA and protein of pancreatic cancer tissues and their adjacent tissues, and normal pancreatic tissues in nude mice were detected by using RT-PCR and immunohistochemical methods. Results The expressions of NGAL mRNA in pancreatic cancer tissues and adjacent tissues were significantly higher than that in normal pancreatic tissues (P < 0.05), and the expression of NGAL mRNA in pancreatic carcinoma tissues was significantly higher than that in para carcinoma tissues (P < 0.05). The strong positive expression rate of NGAL protein in pancreatic carcinoma tissues was significantly higher than thoes in para carcinoma tissues and normal pancreatic tissues (P < 0.05). Conclusions NGAL is highly expressed in pancreatic cancer tissues, and NGAL may be an important regulatory factor in the development of pancreatic cancer.

Citation： LIUYan-sen, YUJing-xi, ZENGHong-wen. Expression of NGAL in Pancreatic Cancer Tissues. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(1): 33-37. doi: 10.7507/1007-9424.20160008 Copy

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2.	Raimondi S, Maisonneuve P, Lowenfels AB. Epidemiology of panc-reatic cancer:an overview. Nat Rev Gastroenterol Hepatol, 2009, 6(12):699-670.
3.	Khan SA, Toledano MB, Taylor-Robinson SD. Epidemiology, risk factors, and pathogenesis of cholangiocarcinoma. HPB (Oxford), 2008, 10(2):77-82.
4.	蒋奶贵, 许丽艳, 郭明洲, 等, 肿瘤相关蛋白NGAL的生物学特征.胃肠病学和肝病学杂志, 2010, 19(4):297-231.
5.	Kjeldsen L, Johnsen AH, Sengeløv H, et al. Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase. J Biol Chem, 1993, 268(14):10425-10432.
6.	Friedl A, Stoesz SP, Buckley P, et al. Neutrophil gelatinase-associated lipocalin in normal and neoplastic human tissues. Cell type-specific pattern of expression. Histochem J, 1999. 31(7):433-441.
7.	Kjeldsen L, Cowland JB, Borregaard N. Human neutrophil gelatinase-associated lipocalin and homologous proteins in rat and mouse. Biochim Biophys Acta, 2000, 1482(1-2):272-283.
8.	Chu ST, Lin HJ, Huang HL, et al. The hydrophobic pocket of 24p3 protein from mouse uterine luminal fluid:fatty acid and retinol binding activity and predicted structural similarity to lipocalins. J Pept Res, 1998, 52(5):390-397.
9.	Bratt T. Lipocalins and cancer. Biochim Biophys Acta, 2000, 1482 (1-2):318-326.
10.	Chakraborty S, Kaur S, Guha S, et al. The multifaceted roles of neutrophil gelatinase associated lipocalin (NGAL) in inflammation and cancer. Biochim Biophys Acta, 2012, 1826(1):129-169.
11.	Fidler IJ. Tumor heterogeneity and the biology of cancer invasion and metastasis. Cancer Res, 1978. 38(9):2651-2660.
12.	马建霞, 司佩任, 吴洪玉, 等, 原位注射法建立人胰腺癌SWl990裸鼠种植瘤模型及高频内镜超声探头对其的监测.中华胰腺病杂志, 2011, 11(1):37-39.
13.	刘娜, 杨文彬, 曹罡, 等, 三种裸鼠胰腺癌建模方法的比较.西安交通大学学报:医学版, 2012, 33(3):300-303.
14.	Linnerth NM, Sirbovan K, Moorehead RA. Use of a transgenic mouse model to identify markers of human lung tumors. Int J Cancer, 2005, 114(6):977-982.
15.	Iacobuzio-Donahue CA, Maitra A, Olsen M, et al. Exploration of global gene expression patterns in pancreatic adenocarcinoma using cDNA microarrays. Am J Pathol, 2003, 162(4):1151-1162.
16.	Fluckinger M, Haas H, Merschak P, et al. Human tear lipocalin exhibits antimicrobial activity by scavenging microbial siderop-hores. Antimicrob Agents Chemother, 2004, 48(9):3367-3372.
17.	Furutani M, Arii S, Mizumoto M, et al. Identification of a neutro-phil gelatinase-associated lipocalin mRNA in human pancreatic cancers using a modified signal sequence trap method. Cancer Lett, 1998, 122(1-2):209-214.
18.	Argani P, Iacobuzio-Donahue C, Ryu B, et al. Mesothelin is overexpressed in the vast majority of ductal adenocarcinomas of the pancreas:identification of a new pancreatic cancer marker by serial analysis of gene expression (SAGE). Clin Cancer Res, 2001, 7(12):3862-3868.
19.	Han H, Bearss DJ, Browne LW, et al. Identification of differentially expressed genes in pancreatic cancer cells using cDNA microarray. Cancer Res, 2002, 62(10):2890-2896.
20.	Maire F, Hammel P, Terris B, et al. Prognosis of malignant intra-ductal papillary mucinous tumours of the pancreas after surgical resection. Comparison with pancreatic ductal adenocarcinoma. Gut, 2002, 51(5):717-722.
21.	Couvelard A, Terris B, Hammel P, et al. Acinar cystic transforma-tion of the pancreas (or acinar cell cystadenoma), a rare and recently described entity. Ann Pathol, 2002, 22(5):397-400.
22.	Iacobuzio-Donahue CA, Maitra A, Olsen M, et al. Exploration of global gene expression patterns in pancreatic adenocarcinoma using cDNA microarrays. Am J Pathol, 2003, 162(4):1151-1162.
23.	Missiaglia E, Blaveri E, Terris B, et al. Analysis of gene expression in cancer cell lines identifies candidate markers for pancreatic tumorigenesis and metastasis. Int J Cancer, 2004, 112(1):100-112.
24.	Tong Z, Kunnumakkara AB, Wang H, et al. Neutrophil gelatinase-associated lipocalin:a novel suppressor of invasion and angiogenesis in pancreatic cancer. Cancer Res, 2008, 68(15):6100-6108.
25.	Pan ZG, Wang B. Anaplastic carcinoma of the pancreas associated with a mucinous cystic adenocarcinoma. A case report and review of the literature. JOP, 2007, 8(6):775-782.
26.	Moniaux N, Chakraborty S, Yalniz M, et al. Early diagnosis of pancreatic cancer:neutrophil gelatinase associated lipocalin as a marker of pancreatic intraepithelial neoplasia. Br J Cancer, 2008, 98(9):1540-1547.

1. Dong M, Dong Q, Zhang H, et al. Expression of Gadd45a and p53 proteins in human pancreatic cancer:potential effects on clinical outcomes. J Surg Oncol, 2007, 95(4):332-336.
2. Raimondi S, Maisonneuve P, Lowenfels AB. Epidemiology of panc-reatic cancer:an overview. Nat Rev Gastroenterol Hepatol, 2009, 6(12):699-670.
3. Khan SA, Toledano MB, Taylor-Robinson SD. Epidemiology, risk factors, and pathogenesis of cholangiocarcinoma. HPB (Oxford), 2008, 10(2):77-82.
4. 蒋奶贵, 许丽艳, 郭明洲, 等, 肿瘤相关蛋白NGAL的生物学特征.胃肠病学和肝病学杂志, 2010, 19(4):297-231.
5. Kjeldsen L, Johnsen AH, Sengeløv H, et al. Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase. J Biol Chem, 1993, 268(14):10425-10432.
6. Friedl A, Stoesz SP, Buckley P, et al. Neutrophil gelatinase-associated lipocalin in normal and neoplastic human tissues. Cell type-specific pattern of expression. Histochem J, 1999. 31(7):433-441.
7. Kjeldsen L, Cowland JB, Borregaard N. Human neutrophil gelatinase-associated lipocalin and homologous proteins in rat and mouse. Biochim Biophys Acta, 2000, 1482(1-2):272-283.
8. Chu ST, Lin HJ, Huang HL, et al. The hydrophobic pocket of 24p3 protein from mouse uterine luminal fluid:fatty acid and retinol binding activity and predicted structural similarity to lipocalins. J Pept Res, 1998, 52(5):390-397.
9. Bratt T. Lipocalins and cancer. Biochim Biophys Acta, 2000, 1482 (1-2):318-326.
10. Chakraborty S, Kaur S, Guha S, et al. The multifaceted roles of neutrophil gelatinase associated lipocalin (NGAL) in inflammation and cancer. Biochim Biophys Acta, 2012, 1826(1):129-169.
11. Fidler IJ. Tumor heterogeneity and the biology of cancer invasion and metastasis. Cancer Res, 1978. 38(9):2651-2660.
12. 马建霞, 司佩任, 吴洪玉, 等, 原位注射法建立人胰腺癌SWl990裸鼠种植瘤模型及高频内镜超声探头对其的监测.中华胰腺病杂志, 2011, 11(1):37-39.
13. 刘娜, 杨文彬, 曹罡, 等, 三种裸鼠胰腺癌建模方法的比较.西安交通大学学报:医学版, 2012, 33(3):300-303.
14. Linnerth NM, Sirbovan K, Moorehead RA. Use of a transgenic mouse model to identify markers of human lung tumors. Int J Cancer, 2005, 114(6):977-982.
15. Iacobuzio-Donahue CA, Maitra A, Olsen M, et al. Exploration of global gene expression patterns in pancreatic adenocarcinoma using cDNA microarrays. Am J Pathol, 2003, 162(4):1151-1162.
16. Fluckinger M, Haas H, Merschak P, et al. Human tear lipocalin exhibits antimicrobial activity by scavenging microbial siderop-hores. Antimicrob Agents Chemother, 2004, 48(9):3367-3372.
17. Furutani M, Arii S, Mizumoto M, et al. Identification of a neutro-phil gelatinase-associated lipocalin mRNA in human pancreatic cancers using a modified signal sequence trap method. Cancer Lett, 1998, 122(1-2):209-214.
18. Argani P, Iacobuzio-Donahue C, Ryu B, et al. Mesothelin is overexpressed in the vast majority of ductal adenocarcinomas of the pancreas:identification of a new pancreatic cancer marker by serial analysis of gene expression (SAGE). Clin Cancer Res, 2001, 7(12):3862-3868.
19. Han H, Bearss DJ, Browne LW, et al. Identification of differentially expressed genes in pancreatic cancer cells using cDNA microarray. Cancer Res, 2002, 62(10):2890-2896.
20. Maire F, Hammel P, Terris B, et al. Prognosis of malignant intra-ductal papillary mucinous tumours of the pancreas after surgical resection. Comparison with pancreatic ductal adenocarcinoma. Gut, 2002, 51(5):717-722.
21. Couvelard A, Terris B, Hammel P, et al. Acinar cystic transforma-tion of the pancreas (or acinar cell cystadenoma), a rare and recently described entity. Ann Pathol, 2002, 22(5):397-400.
22. Iacobuzio-Donahue CA, Maitra A, Olsen M, et al. Exploration of global gene expression patterns in pancreatic adenocarcinoma using cDNA microarrays. Am J Pathol, 2003, 162(4):1151-1162.
23. Missiaglia E, Blaveri E, Terris B, et al. Analysis of gene expression in cancer cell lines identifies candidate markers for pancreatic tumorigenesis and metastasis. Int J Cancer, 2004, 112(1):100-112.
24. Tong Z, Kunnumakkara AB, Wang H, et al. Neutrophil gelatinase-associated lipocalin:a novel suppressor of invasion and angiogenesis in pancreatic cancer. Cancer Res, 2008, 68(15):6100-6108.
25. Pan ZG, Wang B. Anaplastic carcinoma of the pancreas associated with a mucinous cystic adenocarcinoma. A case report and review of the literature. JOP, 2007, 8(6):775-782.
26. Moniaux N, Chakraborty S, Yalniz M, et al. Early diagnosis of pancreatic cancer:neutrophil gelatinase associated lipocalin as a marker of pancreatic intraepithelial neoplasia. Br J Cancer, 2008, 98(9):1540-1547.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of NGAL in Pancreatic Cancer Tissues

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