Effects of Resveratrol on Oxidative Damage in Central Nervous Systerm of Rats with Obstructive Jaundice_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 ZHANGLi-chao ¹ , WANGChen-yu ² , LIUBo ³ , ZHOUYuan-long ¹ , MALi-feng ¹ , LITao ¹ ,  LIUHuai-jun ⁴

1. Department of Minimally Invasive Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China;
2. Medical Department of First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, Hebei Province, China;
3. Department of Oncosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China;
4. Imaging Department, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China;

Corresponding author：

LIUHuai-jun, Email: 36202310@qq.com

Keywords：

Obstructive jaundice; Oxidative stress; Resveratrol

DOI：

10.7507/1007-9424.20160011

Video：

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Objective To investigate the effects of different levels of resveratrol on oxidative stress injury in central nervous system of rats with obstructive jaundice and its protective effect and mechanism of oxidative stress injury. Methods Thirty two female SD rats of 6 weeks old were used as experimental object. The animals were randomly divided into four groups, 8 rats in each group. Sham operation group (SO group), the common bile duct were seperated without ligation; while the models of obstructive jaundice of obstructive jaundice group (OJ group), obstructive jaundice+low dose resvera-trol (L-Res)treatment group (OJ+L-Res group), and obstructive jaundice+high dose resveratrol (H-Res) treatment group (OJ+H-Res group) were established by operation. After the operation, the rats in OJ+L-Res group and OJ+H-Res group were treated with different doses of resveratrol, the rats in SO group and OJ group were given the same dose of normal saline. On the 14th day after operation, blood were tested for total bilirubin (TBIL), direct bilirubin (DBIL), ALT, and AST. And cerebral cortex specimen were collected, then malondialdehyde (MDA), total superoxidedismutase (T-SOD) activity, and HO-1 protein expression in the rats brain of the four groups were measured. Results The levels rise of TBIL and DBIL after modeling suggested that obstructive jaundice model were estabilshed successfully, but there was no significant difference among the OJ group, OJ+L-Res group, and OJ+H-Res group. In the OJ group, OJ+L-Res group, and OJ+H-Res group, the levels of ALT, AST, and MDA were increased while levels of T-SOD and HO-1 protein expression were decreased when compared with SO group(P < 0.05). Among the OJ group, OJ+L-Res group, and OJ+H-Res group, levels of ALT, AST and MDA were lower in the treatment groups than in the OJ group(P < 0.05), while levels of T-SOD and HO-1 protein expression which reflects the oxidative stress were higher in the treatment group(P < 0.05). Different doses of resveratrol had different effects on T-SOD and HO-1 protein expression with statisticl significance (P < 0.05). Conclusions Resveratrol have little effect on TBIL and DBIL of obstrctive jaundice rats, but it can protect the liver function, and it has antioxidant properties of decreasing MDA and incresing SOD and HO-1 protein expression levels in the cerebral cortex cells of obstructive jaundiced rats.

Citation： ZHANGLi-chao, WANGChen-yu, LIUBo, ZHOUYuan-long, MALi-feng, LITao, LIUHuai-jun. Effects of Resveratrol on Oxidative Damage in Central Nervous Systerm of Rats with Obstructive Jaundice. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(1): 42-47. doi: 10.7507/1007-9424.20160011 Copy

1.	Chroni E, Patsoukis N, Karageorgos N, et al. Brain oxidative stress induced by obstructive jaundice in rats. J Neuropathol Exp Neurol, 2006, 65(2):193-198.
2.	Haider UG, Sorescu D, Griendling KK, et al. Resveratrol increases serine15-phosphorylated but transcriptionally impaired p53 and induces a reversible DNA replication block in serum-activated vascular smooth muscle cells. Mol Pharmacol, 2003, 63(4):925-932.
3.	余水平, 李坚, 侯冰宗, 等.急慢性大鼠梗阻性黄疸模型的建立.世界华人消化杂志, 2011, 19(12):1285-1289.
4.	Faropoulos K, Chroni E, Assimakopoulos SF, et al. Altered occludin expression in brain capillaries induced by obstructive jaundice in rats. Brain Res, 2010, 1325:121-127.
5.	Tang J, Yan H, Zhuang S. Inflammation and oxidative stress in obesity-related glomerulopathy. Int J Nephrol, 2012, 2012:608397.
6.	Mokni M, Elkahoui S, Limam F, et al. Effect of resveratrol on antioxidant enzyme activities in the brain of healthy rat. Neurochem Res, 2007, 32(6):981-987.
7.	Arichi H, Kimura Y, Okuda H, et al. Effects of stilbene components of the roots of polygonum cuspidatum sieb. et Zucc. on lipid meta-bolism. Chem Pharm Bull (Tokyo), 1982, 30(5):1766-1770.
8.	Leonard SS, Xia C, Jiang BH, et al. Resveratrol scavenges reactive oxygen species and effects radical-induced cellular responses. Biochem Biophys Res Commun, 2003, 309(4):1017-1026.
9.	侯媛媛刘丽萍, 王建斌, 等.白藜芦醇对力竭游泳大鼠肝脏组织的保护作用研究.河北体育学院学报, 2012, 26(5):72-76.
10.	Kitada M, Kume S, Imaizumi N, et al. Resveratrol improves oxidative stress and protects against diabetic nephropathy through normalization of mn-sod dysfunction in ampk/sirt1-independent pathway. Diabetes, 2011, 60(2):634-643.
11.	Start C. Some valuable lessons we've learned from mda members. J Mich Dent Assoc, 2011, 93(9):24.
12.	李东阳, 张天成, 让慰清, 等.烹调油烟对大鼠肺组织SOD和MDA及骨髓MN变化的研究.卫生研究, 1998, 2 7(6):382-384.
13.	Nourani MR, Yazdani S, Roudkenar MH, et al. Ho1 mrna and protein do not change in parallel in bronchial biopsies of patients after long term exposure to sulfur mustard. Gene Regul Syst Bio, 2009, 4:83-90.
14.	Shah ZA, Li RC, Ahmad AS, et al. The flavanol (-)-epicatechin prevents stroke damage through the nrf2/ho1 pathway. J Cereb Blood Flow Metab, 2010, 30(12):1951-1961.
15.	Hayashi S, Takamiya R, Yamaguchi T, et al. Induction of heme oxygenase-1 suppresses venular leukocyte adhesion elicited by oxidative stress:role of bilirubin generated by the enzyme. Circ Res, 1999, 85(8):663-671.
16.	Wagener FA, da Silva JL, Farley T, et al. Differential effects of heme oxygenase isoforms on heme mediation of endothelial intracellular adhesion molecule 1 expression. J Pharmacol Exp Ther, 1999, 291(1):416-423.
17.	Zhuang H, Kim YS, Koehler RC, et al. Potential mechanism by which resveratrol, a red wine constituent, protects neurons. Ann N Y Acad Sci, 2003, 993:276-286; discussion 287-278.
18.	Kavas GO, Ayral PA, Elhan AH. The effects of resveratrol on oxidant/antioxidant systems and their cofactors in rats. Adv Clin Exp Med, 2013, 22(2):151-155.
19.	Bastianetto S, Zheng WH, Quirion R. Neuroprotective abilities of resveratrol and other red wine constituents against nitric oxide-related toxicity in cultured hippocampal neurons. Br J Pharmacol, 2000, 131(4):711-720.
20.	Hamlaoui S, Mokni M, Limam N, et al. Resveratrol protects against acute chemotherapy toxicity induced by doxorubucin in rat erythrocyte and plasma. J Physiol Pharmacol, 2012, 63(3):293-301.
21.	Huang HM, Liang YC, Cheng TH, et al. Potential mechanism of blood vessel protection by resveratrol, a component of red wine. Ann N Y Acad Sci, 2005, 1042:349-356.
22.	Sakata Y, Zhuang H, Kwansa H, et al. Resveratrol protects against experimental stroke:Putative neuroprotective role of heme oxyge-nase 1. Exp Neurol, 2010, 224(1):325-329.
23.	Kim JW, Lim SC, Lee MY, et al. Inhibition of neointimal formation by trans-resveratrol:Role of phosphatidyl inositol 3-kinase-depen-dent nrf2 activation in heme oxygenase-1 induction. Mol Nutr Food Res, 2010, 54(10):1497-1505.
24.	Quincozes-Santos A, Bobermin LD, Latini A, et al. Resveratrol protects c6 astrocyte cell line against hydrogen peroxide-induced oxidative stress through heme oxygenase 1. PLoS One, 2013, 8(5):e64372.
25.	McBurney MW, Yang X, Jardine K, et al. The mammalian sir2alpha protein has a role in embryogenesis and gametogenesis. Mol Cell Biol, 2003, 23(1):38-54.

1. Chroni E, Patsoukis N, Karageorgos N, et al. Brain oxidative stress induced by obstructive jaundice in rats. J Neuropathol Exp Neurol, 2006, 65(2):193-198.
2. Haider UG, Sorescu D, Griendling KK, et al. Resveratrol increases serine15-phosphorylated but transcriptionally impaired p53 and induces a reversible DNA replication block in serum-activated vascular smooth muscle cells. Mol Pharmacol, 2003, 63(4):925-932.
3. 余水平, 李坚, 侯冰宗, 等.急慢性大鼠梗阻性黄疸模型的建立.世界华人消化杂志, 2011, 19(12):1285-1289.
4. Faropoulos K, Chroni E, Assimakopoulos SF, et al. Altered occludin expression in brain capillaries induced by obstructive jaundice in rats. Brain Res, 2010, 1325:121-127.
5. Tang J, Yan H, Zhuang S. Inflammation and oxidative stress in obesity-related glomerulopathy. Int J Nephrol, 2012, 2012:608397.
6. Mokni M, Elkahoui S, Limam F, et al. Effect of resveratrol on antioxidant enzyme activities in the brain of healthy rat. Neurochem Res, 2007, 32(6):981-987.
7. Arichi H, Kimura Y, Okuda H, et al. Effects of stilbene components of the roots of polygonum cuspidatum sieb. et Zucc. on lipid meta-bolism. Chem Pharm Bull (Tokyo), 1982, 30(5):1766-1770.
8. Leonard SS, Xia C, Jiang BH, et al. Resveratrol scavenges reactive oxygen species and effects radical-induced cellular responses. Biochem Biophys Res Commun, 2003, 309(4):1017-1026.
9. 侯媛媛刘丽萍, 王建斌, 等.白藜芦醇对力竭游泳大鼠肝脏组织的保护作用研究.河北体育学院学报, 2012, 26(5):72-76.
10. Kitada M, Kume S, Imaizumi N, et al. Resveratrol improves oxidative stress and protects against diabetic nephropathy through normalization of mn-sod dysfunction in ampk/sirt1-independent pathway. Diabetes, 2011, 60(2):634-643.
11. Start C. Some valuable lessons we've learned from mda members. J Mich Dent Assoc, 2011, 93(9):24.
12. 李东阳, 张天成, 让慰清, 等.烹调油烟对大鼠肺组织SOD和MDA及骨髓MN变化的研究.卫生研究, 1998, 2 7(6):382-384.
13. Nourani MR, Yazdani S, Roudkenar MH, et al. Ho1 mrna and protein do not change in parallel in bronchial biopsies of patients after long term exposure to sulfur mustard. Gene Regul Syst Bio, 2009, 4:83-90.
14. Shah ZA, Li RC, Ahmad AS, et al. The flavanol (-)-epicatechin prevents stroke damage through the nrf2/ho1 pathway. J Cereb Blood Flow Metab, 2010, 30(12):1951-1961.
15. Hayashi S, Takamiya R, Yamaguchi T, et al. Induction of heme oxygenase-1 suppresses venular leukocyte adhesion elicited by oxidative stress:role of bilirubin generated by the enzyme. Circ Res, 1999, 85(8):663-671.
16. Wagener FA, da Silva JL, Farley T, et al. Differential effects of heme oxygenase isoforms on heme mediation of endothelial intracellular adhesion molecule 1 expression. J Pharmacol Exp Ther, 1999, 291(1):416-423.
17. Zhuang H, Kim YS, Koehler RC, et al. Potential mechanism by which resveratrol, a red wine constituent, protects neurons. Ann N Y Acad Sci, 2003, 993:276-286; discussion 287-278.
18. Kavas GO, Ayral PA, Elhan AH. The effects of resveratrol on oxidant/antioxidant systems and their cofactors in rats. Adv Clin Exp Med, 2013, 22(2):151-155.
19. Bastianetto S, Zheng WH, Quirion R. Neuroprotective abilities of resveratrol and other red wine constituents against nitric oxide-related toxicity in cultured hippocampal neurons. Br J Pharmacol, 2000, 131(4):711-720.
20. Hamlaoui S, Mokni M, Limam N, et al. Resveratrol protects against acute chemotherapy toxicity induced by doxorubucin in rat erythrocyte and plasma. J Physiol Pharmacol, 2012, 63(3):293-301.
21. Huang HM, Liang YC, Cheng TH, et al. Potential mechanism of blood vessel protection by resveratrol, a component of red wine. Ann N Y Acad Sci, 2005, 1042:349-356.
22. Sakata Y, Zhuang H, Kwansa H, et al. Resveratrol protects against experimental stroke:Putative neuroprotective role of heme oxyge-nase 1. Exp Neurol, 2010, 224(1):325-329.
23. Kim JW, Lim SC, Lee MY, et al. Inhibition of neointimal formation by trans-resveratrol:Role of phosphatidyl inositol 3-kinase-depen-dent nrf2 activation in heme oxygenase-1 induction. Mol Nutr Food Res, 2010, 54(10):1497-1505.
24. Quincozes-Santos A, Bobermin LD, Latini A, et al. Resveratrol protects c6 astrocyte cell line against hydrogen peroxide-induced oxidative stress through heme oxygenase 1. PLoS One, 2013, 8(5):e64372.
25. McBurney MW, Yang X, Jardine K, et al. The mammalian sir2alpha protein has a role in embryogenesis and gametogenesis. Mol Cell Biol, 2003, 23(1):38-54.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Resveratrol on Oxidative Damage in Central Nervous Systerm of Rats with Obstructive Jaundice

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