Role of Alpha Adrenoceptor on Modulating Water in Lung of Severe Acute Pancreatitis Rat Model_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 XUJin , XUWei-xue , LIANGHan-li , LIANGJie-jia , WANGHai-nan , ZHANGWei-ming

Department of Pancreatic Surgery, Affiliated Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China;

Corresponding author：

XUJin, Email: xujinwang@163.com

Keywords：

Severe acute pancreatitis; Alveolar fluid clearance; Pulmonary edema; Alpha adrenoceptor

DOI：

10.7507/1007-9424.20160045

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To explore effect of α-adrenoceptor on modulating water of lung in severe acute pancrea-titis (SAP) rat. Methods The SD rats were randomly divided into sham operation group (n=5) and SAP group,the SAP group was divided into subgroups of SAP-4 h (n=5) and SAP-24 h (n=5).SAP model was made by injecting taurocholate into bilopancreatic duct.The wet-to-dry ratio,alveolar fluid clearance (AFC),and AFC affected by α1-adrenoceptor inhibitor-prazosin and α2-adrenoceptor inhibitor-yohimbine separately or together were measured in the lungs.The α1-adrenoceptor and α2-adrenoceptor mRNA expressions in the lungs tissues were measured by real-time PCR. Results ① The wet-to-dry ratios in the SAP-4 h group and SAP-24 h group were obviously decreased as compared with the sham operation group (P<0.05),which in the SAP-24 h group was significantly lower than that in the SAP-4 h group (P<0.05).② The AFCs in the SAP-4 h group and SAP-24 h group were obviously increased as compared with the sham operation group (P<0.05).The AFCs in the SAP with α1-adrenoceptor inhibitor-prazosin or α2-adrenocpetor inhibitor-yohimbine or prazosin combined with yohimbine were all obviously decreased as compared with the SAP group (P<0.05).③ The α1 adrenoceptor and α2 adrenoceptor mRNAs in the SAP-4 h group and SAP-24 h group were obviously increased as compared with the sham operation group (P<0.05). Conclusion AFC might be modulated by α-adrenoceptor in SAP rat.

Citation： XUJin, XUWei-xue, LIANGHan-li, LIANGJie-jia, WANGHai-nan, ZHANGWei-ming. Role of Alpha Adrenoceptor on Modulating Water in Lung of Severe Acute Pancreatitis Rat Model. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(2): 167-171. doi: 10.7507/1007-9424.20160045 Copy

1.	Kan S, Zhou H, Jin C, et al. Effects of PDTC on NF-κB expression and apoptosis in rats with severe acute pancreatitis-associated lung injury. Int J Clin Exp Med, 2015, 8(3):3258-3270.
2.	Surbatović M, Jovanović K, Radaković S, et al. Pathophysiological aspects of severe acute pancreatitis-associated lung injury. Srp Arh Celok Lek, 2005, 133(1/2):76-81.
3.	Giakoustidis A, Mudan SS, Giakoustidis D. Dissecting the stress activating signaling pathways in acute pancreatitis. Hepatogastroen-terology, 2010, 57(99-100):653-656.
4.	Qu Z, Jiang Y, Wu BQ, et al. Cystathionine-gamma-lyase inhibitor attenuates acute lung injury induced by acute pancreatitis in rats. Arch Med Sci, 2014, 10(4):825-829.
5.	Gotts JE, Matthay MA. Endogenous and exogenous cell-based pathways for recovery from acute respiratory distress syndrome. Clin Chest Med, 2014, 35(4):797-809.
6.	Gao Smith F, Perkins GD, Gates S, et al. Effect of intravenous β-2 agonist treatment on clinical outcomes in acute respiratory distress syndrome (BALTI-2):a multicentre, randomised controlled trial. Lancet, 2012, 379(9812):229-235.
7.	National Heart, Lung, and Blood Institute ARDS Clinical Trials Network; Truwit JD, Bernard GR, Steingrub J, et al. Rosuvastatin for sepsis-associated acute respiratory distress syndrome. N Engl J Med, 2014, 370(23):2191-2200.
8.	Wagener BM, Roux J, Carles M, et al. Synergistic inhibition of β2-adrenergic receptor-mediated alveolar epithelial fluid transport by interleukin-8 and transforming growth factor-β. Anesthesiology, 2015, 122(5):1084-1092.
9.	Huang B, Wang DX, Deng W. Protective effects of dexamethasone on early acute lung injury induced by oleic acid in rats. Int J Clin Exp Med, 2014, 7(12):4698-4709.
10.	Wang Q, Zheng X, Cheng Y, et al. Resolvin D1 stimulates alveolar fluid clearance through alveolar epithelial sodium channel, Na, K-ATPase via ALX/cAMP/PI3K pathway in lipopolysaccharide-induced acute lung injury. J Immunol, 2014, 192(8):3765-3777.
11.	Lankisch PG, Apte M, Banks PA. Acute pancreatitis. Lancet, 2015, 386(9988):85-96.
12.	Algül H, Treiber M, Lesina M, et al. Pancreas-specific RelA/p65 truncation increases susceptibility of acini to inflammation-associated cell death following cerulein pancreatitis. J Clin Invest, 2007, 117(6):1490-1501.
13.	Talukdar R, Vege SS. Acute pancreatitis. Curr Opin Gastroenterol, 2015, 31(5):374-379.
14.	Sadowski SM, Andres A, Morel P, et al. Epidural anesthesia improves pancreatic perfusion and decreases the severity of acute pancreatitis. World J Gastroenterol, 2015, 21(43):12448-12456.
15.	Cui HX, Xu JY, Li MQ. Efficacy of continuous renal replacement therapy in the treatment of severe acute pancreatitis associated acute respiratory distress syndrome. Eur Rev Med Pharmacol Sci, 2014, 18(17):2523-2526.
16.	Matthay MA, Ware LB. Resolution of alveolar edema in acute respira-tory distress syndrome. Am J Respir Crit Care Med, 2015, 192(2):124-125.
17.	Kangelaris KN, Prakash A, Liu KD, et al. Increased expression of neutrophil-related genes in patients with early sepsis-induced ARDS. Am J Physiol Lung Cell Mol Physiol, 2015, 308(11):L1102-L1113.
18.	Maron-Gutierrez T, Laffey JG, Pelosi P, et al. Cell-based therapies for the acute respiratory distress syndrome. Curr Opin Crit Care, 2014, 20(1):122-131.
19.	Gotts JE, Abbott J, Matthay MA. Influenza causes prolonged disruption of the alveolar-capillary barrier in mice unresponsive to mesenchymal stem cell therapy. Am J Physiol Lung Cell Mol Physiol, 2014, 307(5):L395-L406.
20.	Gao Z, Xu J, Sun D, et al. Traditional Chinese medicine, Qing Ying Tang, ameliorates the severity of acute lung injury induced by severe acute pancreatitis in rats via the upregulation of aquaporin-1. Exp Ther Med, 2014, 8(6):1819-1824.
21.	Kondo T, Chen F, Ohsumi A, et al. β2-Adrenoreceptor agonist inhalation during Ex vivo lung perfusion attenuates lung injury. Ann Thorac Surg, 2015, 100(2):480-486.
22.	Woods PS, Tazi MF, Chesarino NM, et al. TGF-β-induced IL-6 prevents development of acute lung injury in influenza A virus-infected F508del CFTR-heterozygous mice. Am J Physiol Lung Cell Mol Physiol, 2015, 308(11):L1136-L1144.
23.	Budinger GR, Mutlu GM. β2-agonists and acute respiratory distress syndrome. Am J Respir Crit Care Med, 2014, 189(6):624-625.

1. Kan S, Zhou H, Jin C, et al. Effects of PDTC on NF-κB expression and apoptosis in rats with severe acute pancreatitis-associated lung injury. Int J Clin Exp Med, 2015, 8(3):3258-3270.
2. Surbatović M, Jovanović K, Radaković S, et al. Pathophysiological aspects of severe acute pancreatitis-associated lung injury. Srp Arh Celok Lek, 2005, 133(1/2):76-81.
3. Giakoustidis A, Mudan SS, Giakoustidis D. Dissecting the stress activating signaling pathways in acute pancreatitis. Hepatogastroen-terology, 2010, 57(99-100):653-656.
4. Qu Z, Jiang Y, Wu BQ, et al. Cystathionine-gamma-lyase inhibitor attenuates acute lung injury induced by acute pancreatitis in rats. Arch Med Sci, 2014, 10(4):825-829.
5. Gotts JE, Matthay MA. Endogenous and exogenous cell-based pathways for recovery from acute respiratory distress syndrome. Clin Chest Med, 2014, 35(4):797-809.
6. Gao Smith F, Perkins GD, Gates S, et al. Effect of intravenous β-2 agonist treatment on clinical outcomes in acute respiratory distress syndrome (BALTI-2):a multicentre, randomised controlled trial. Lancet, 2012, 379(9812):229-235.
7. National Heart, Lung, and Blood Institute ARDS Clinical Trials Network; Truwit JD, Bernard GR, Steingrub J, et al. Rosuvastatin for sepsis-associated acute respiratory distress syndrome. N Engl J Med, 2014, 370(23):2191-2200.
8. Wagener BM, Roux J, Carles M, et al. Synergistic inhibition of β2-adrenergic receptor-mediated alveolar epithelial fluid transport by interleukin-8 and transforming growth factor-β. Anesthesiology, 2015, 122(5):1084-1092.
9. Huang B, Wang DX, Deng W. Protective effects of dexamethasone on early acute lung injury induced by oleic acid in rats. Int J Clin Exp Med, 2014, 7(12):4698-4709.
10. Wang Q, Zheng X, Cheng Y, et al. Resolvin D1 stimulates alveolar fluid clearance through alveolar epithelial sodium channel, Na, K-ATPase via ALX/cAMP/PI3K pathway in lipopolysaccharide-induced acute lung injury. J Immunol, 2014, 192(8):3765-3777.
11. Lankisch PG, Apte M, Banks PA. Acute pancreatitis. Lancet, 2015, 386(9988):85-96.
12. Algül H, Treiber M, Lesina M, et al. Pancreas-specific RelA/p65 truncation increases susceptibility of acini to inflammation-associated cell death following cerulein pancreatitis. J Clin Invest, 2007, 117(6):1490-1501.
13. Talukdar R, Vege SS. Acute pancreatitis. Curr Opin Gastroenterol, 2015, 31(5):374-379.
14. Sadowski SM, Andres A, Morel P, et al. Epidural anesthesia improves pancreatic perfusion and decreases the severity of acute pancreatitis. World J Gastroenterol, 2015, 21(43):12448-12456.
15. Cui HX, Xu JY, Li MQ. Efficacy of continuous renal replacement therapy in the treatment of severe acute pancreatitis associated acute respiratory distress syndrome. Eur Rev Med Pharmacol Sci, 2014, 18(17):2523-2526.
16. Matthay MA, Ware LB. Resolution of alveolar edema in acute respira-tory distress syndrome. Am J Respir Crit Care Med, 2015, 192(2):124-125.
17. Kangelaris KN, Prakash A, Liu KD, et al. Increased expression of neutrophil-related genes in patients with early sepsis-induced ARDS. Am J Physiol Lung Cell Mol Physiol, 2015, 308(11):L1102-L1113.
18. Maron-Gutierrez T, Laffey JG, Pelosi P, et al. Cell-based therapies for the acute respiratory distress syndrome. Curr Opin Crit Care, 2014, 20(1):122-131.
19. Gotts JE, Abbott J, Matthay MA. Influenza causes prolonged disruption of the alveolar-capillary barrier in mice unresponsive to mesenchymal stem cell therapy. Am J Physiol Lung Cell Mol Physiol, 2014, 307(5):L395-L406.
20. Gao Z, Xu J, Sun D, et al. Traditional Chinese medicine, Qing Ying Tang, ameliorates the severity of acute lung injury induced by severe acute pancreatitis in rats via the upregulation of aquaporin-1. Exp Ther Med, 2014, 8(6):1819-1824.
21. Kondo T, Chen F, Ohsumi A, et al. β2-Adrenoreceptor agonist inhalation during Ex vivo lung perfusion attenuates lung injury. Ann Thorac Surg, 2015, 100(2):480-486.
22. Woods PS, Tazi MF, Chesarino NM, et al. TGF-β-induced IL-6 prevents development of acute lung injury in influenza A virus-infected F508del CFTR-heterozygous mice. Am J Physiol Lung Cell Mol Physiol, 2015, 308(11):L1136-L1144.
23. Budinger GR, Mutlu GM. β2-agonists and acute respiratory distress syndrome. Am J Respir Crit Care Med, 2014, 189(6):624-625.

Previous Article
肝血管瘤手术血流阻断方法的选择
Next Article
Brain Injury Biomarkers Monitoring for the Patients during Extracorporeal Membrane Oxygenation: A Systematic Review

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Role of Alpha Adrenoceptor on Modulating Water in Lung of Severe Acute Pancreatitis Rat Model

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content