Effect of Echinococcus Cyst Fluid on Proliferation and Cell Cycle Progression of Rat Hepatic Stellate Cell_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 RENLi ^1,2 , WANGZhi-xin ^1,2 , LIUZhi-sheng ³ , HOULi-zhao ^1,2 , YANGDANCairang ^1,2 , WANGHai-jiu ^1,2 , ZHOUYing ^1,2 , WENHao ⁴ ,  FANHai-ning ^1,2

1. Department of Hepatopancreatobiliary Surgery, Affiliated Hospital of Qinghai University, Xining 810001, Qinghai Province, China;
2. Qinghai Province Key Laboratory of Hydatid Disease Research, Xining 810001, Qinghai Province, China;
3. Medical College of Qinghai University, Xining 810001, Qinghai Province, China;
4. The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang, China;

Corresponding author：

FANHai-ning, Email: fanhaining@medmail.com.cn

Keywords：

Echinococcus cyst fluid; HSC-T6 cell; Proliferation; Cell cycle

DOI：

10.7507/1007-9424.20160137

Video：

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Objective To observe effect of echinococcus cyst fluid on proliferation and cell cycle progression of rat hepatic stellate cells (HSC-T6), and to preliminarily reveal a new mechanisms of pro-fibrogenic effect of alveolar echinococcosis. Methods HSC-T6 cells were treated with different concentrations (0.00-0.90 mg/mL) of echinococcus cyst fluid. Then, the morphological changes were observed under the inverted microscope, the impacts on proliferation and cell cycle were tested by the CCK-8 assay and flow cytometry respectively. Results After treated by echinococcus cyst fluids with different concentrations (0.00-0.90 mg/mL) for 24 h, the most cells shrinked fusiform shape with more slender synapses, and the proliferation activities were increased with the concentration of echinococcus cyst fluid increasing when it was higher than 0.05 mg/mL (P<0.05), the proportion of G0/G1 was decreased (P<0.05) and those of S and G2/M were increased (P<0.05) with the concentration of echinococcus cyst fluid increasing. Conclusions Echinococcus cyst fluid could promote proliferation of HSC-T6 cells in a dose-dependent manner, which might due to its impact on cell cycle progression. Therefore, alveolar echinococcosis might promote hepatic fibrosis through regulating hepatic stellate cells, but further research on detail needs to be done in future.

Citation： RENLi, WANGZhi-xin, LIUZhi-sheng, HOULi-zhao, YANGDANCairang, WANGHai-jiu, ZHOUYing, WENHao, FANHai-ning. Effect of Echinococcus Cyst Fluid on Proliferation and Cell Cycle Progression of Rat Hepatic Stellate Cell. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(5): 517-520. doi: 10.7507/1007-9424.20160137 Copy

1.	Liu YH, Wang XG, Chen YT, et al. Computer tomography of liver in alveolar echinococcosis treated with albendazole. Trans R Soc Trop Med Hyg, 1993, 87(3):319-321.
2.	Craig PS, Li T, Qiu J, et al. Echinococcosis and Tibetan communities. Emerg Infect Dis, 2008, 14(10):1674-1675.
3.	Morakote N, Thamprasert K, Lojanapiwat B, et al. Cystic echinococcosis in Thailand with a special note on detection by serology in one family. Southeast Asian J Trop Med Public Health, 2007, 38(5):796-798.
4.	Maoz D, Greif F, Chen J. Operative treatment of hepatic hydatid cysts:a single center experience in Israel, a nonendemic country. ISRN Surg, 2013, 2013:276807.
5.	Eckert J, Conraths FJ, Tackmann K. Echinococcosis:an emerging or re-emerging zoonosis? Int J Parasitol, 2000, 30(12-13):1283-1294.
6.	Polat P, Kantarci M, Alper F, et al. Hydatid disease from head to toe. Radiographics, 2003, 23(2):475-494.
7.	Yang YR, Craig PS, Sun T, et al. Echinococcosis in Ningxia Hui Autonomous Region, northwest China. Trans R Soc Trop Med Hyg, 2008, 102(4):319-328.
8.	Jiang CP, Don M, Jones M. Liver alveolar echinococcosis in China:clinical aspect with relative basic research. World J Gastroenterol, 2005, 11(30):4611-4617.
9.	周晓涛, 林仁勇, 张亚楼, 等. 泡球蚴囊液对原代培养大鼠肝细胞增殖影响的初步研究. 中国病原生物学杂志, 2008, 3(3):197-199.
10.	Geerts A. On the origin of stellate cells:mesodermal, endodermal or neuroectodermal. J Hepatol, 2004, 40(2):331-334.
11.	温浩, 徐明谦. 实用包虫病学. 北京:科学出版社, 2007.
12.	Parsons CJ, Takashima M, Rippe RA. Molecular mechanisms of hepatic fibrogenesis. J Gastroenterol Hepatol, 2008, 23(3):501-502.
13.	Tacke F, Weiskirchen R. Update on hepatic stellate cells:pathogenic role in liver fibrosis and novel isolation techniques. Expert Rev Gastroenterol Hepatol, 2012, 6(1):67-80.
14.	Ducal F, Moreno-Cuevas JE, Gonzalez-Garza MT, et al. Liver fibrosis and mechanisms of the protective action of medicinal plants targeting inflammation and the immune response. Int J Inflam, 2015, 2015:943497.
15.	Gonzalo T, Beljaars L, van de Bovenkamp M, et al. Local inhibition of liver fibrosis by specific delivery of a platelet-derived growth factorkinase inhibitor to hepatic stellate cells. J Pharmacol Exp Ther, 2007, 321(3):856-865.
16.	Parsons CJ, Takashima M, Rippe RA. Molecular mechanisms of hepatic fibrogenesis. J Gastroenterol Hepatol, 2007, 22 Suppl 1:S79-S84.
17.	Kisseleva T, Brenner DA. Role of hepatic stellate cells in fibrogenesis and the reversal of fibrosis. J Gastroenterol Hepatol, 2007, 22 Suppl 1:S73-S78.
18.	Rockey DC. Antifibrotic therapy in chronic liver disease. Clin Gastroenterol Hepatol, 2005, 3(2):95-107.
19.	李婧婷, 汪晖, 廖长秀. 肝星状细胞激活的分子机制与抗肝纤维化的治疗. 武汉大学学报, 2007, 28(2):256-261.
20.	Li J, Qiu SJ, She WM, et al. Significance of the balance between regulatory T (Treg) and T helper 17(Th17) cells during hepatitis B virus related liver fibrosis. PLoS One, 2012, 7(6):e39307.
21.	Florian W, Guido H, Ralf W, et al. Ito cells are liver-resident antigen-presenting cells for activating T cell responses. Immunity, 2007, 26(1):117-129.
22.	Weiskirchen R, Tacke F. Cellular and molecular functions of hepatic stellate cells in inflammatory responses and liver immunology. Hepatobiliary Surg Nutr, 2014, 3(6):344-363.
23.	Yu MC, Chen CH, Liang X, et al. Inhibition of T-cell responses by hepatic stellate cells via B7-H1-mediated T-cell apoptosis in mice. Hepatology, 2004, 40(6):1312-1321.
24.	Chen CH, Liang-Mou K, Chang Y, et al. In vivo immune modulatory activity of hepatic stellate cells in mice. Hepatology, 2006, 44(5):1171-1181.
25.	Guoping J, Horng-Ren Y, Lianfu W, et al. Hepatic stellate cells preferentially expand allogeneic CD4+ CD25+ FoxP3+ regulatory T cells in an IL-2-dependent manner. Transplantation, 2009, 86(11):1492-1502.
26.	Yang HR, Hong-Shuie C, Gu X, et al. Mechanistic insights into immunomodulation by hepatic stellate cells in mice:a critical role of interferon-gamma signaling. Hepatology, 2009, 50(6):1981-1991.
27.	Bastian H, Ormandy LA, Matthias B, et al. A new population of myeloid-derived suppressor cells in hepatocellular carcinoma patients induces CD4(+)CD25(+)Foxp3(+) T cells. Gastroenterology, 2008, 135(1):234-243.
28.	Chen CH, Yu MC, Lu L, et al.215 Hepatic stellate cells regulate immune responses by induction of activated T cells apoptosis. Hepatology, 2003, 38(38):260.

1. Liu YH, Wang XG, Chen YT, et al. Computer tomography of liver in alveolar echinococcosis treated with albendazole. Trans R Soc Trop Med Hyg, 1993, 87(3):319-321.
2. Craig PS, Li T, Qiu J, et al. Echinococcosis and Tibetan communities. Emerg Infect Dis, 2008, 14(10):1674-1675.
3. Morakote N, Thamprasert K, Lojanapiwat B, et al. Cystic echinococcosis in Thailand with a special note on detection by serology in one family. Southeast Asian J Trop Med Public Health, 2007, 38(5):796-798.
4. Maoz D, Greif F, Chen J. Operative treatment of hepatic hydatid cysts:a single center experience in Israel, a nonendemic country. ISRN Surg, 2013, 2013:276807.
5. Eckert J, Conraths FJ, Tackmann K. Echinococcosis:an emerging or re-emerging zoonosis? Int J Parasitol, 2000, 30(12-13):1283-1294.
6. Polat P, Kantarci M, Alper F, et al. Hydatid disease from head to toe. Radiographics, 2003, 23(2):475-494.
7. Yang YR, Craig PS, Sun T, et al. Echinococcosis in Ningxia Hui Autonomous Region, northwest China. Trans R Soc Trop Med Hyg, 2008, 102(4):319-328.
8. Jiang CP, Don M, Jones M. Liver alveolar echinococcosis in China:clinical aspect with relative basic research. World J Gastroenterol, 2005, 11(30):4611-4617.
9. 周晓涛, 林仁勇, 张亚楼, 等. 泡球蚴囊液对原代培养大鼠肝细胞增殖影响的初步研究. 中国病原生物学杂志, 2008, 3(3):197-199.
10. Geerts A. On the origin of stellate cells:mesodermal, endodermal or neuroectodermal. J Hepatol, 2004, 40(2):331-334.
11. 温浩, 徐明谦. 实用包虫病学. 北京:科学出版社, 2007.
12. Parsons CJ, Takashima M, Rippe RA. Molecular mechanisms of hepatic fibrogenesis. J Gastroenterol Hepatol, 2008, 23(3):501-502.
13. Tacke F, Weiskirchen R. Update on hepatic stellate cells:pathogenic role in liver fibrosis and novel isolation techniques. Expert Rev Gastroenterol Hepatol, 2012, 6(1):67-80.
14. Ducal F, Moreno-Cuevas JE, Gonzalez-Garza MT, et al. Liver fibrosis and mechanisms of the protective action of medicinal plants targeting inflammation and the immune response. Int J Inflam, 2015, 2015:943497.
15. Gonzalo T, Beljaars L, van de Bovenkamp M, et al. Local inhibition of liver fibrosis by specific delivery of a platelet-derived growth factorkinase inhibitor to hepatic stellate cells. J Pharmacol Exp Ther, 2007, 321(3):856-865.
16. Parsons CJ, Takashima M, Rippe RA. Molecular mechanisms of hepatic fibrogenesis. J Gastroenterol Hepatol, 2007, 22 Suppl 1:S79-S84.
17. Kisseleva T, Brenner DA. Role of hepatic stellate cells in fibrogenesis and the reversal of fibrosis. J Gastroenterol Hepatol, 2007, 22 Suppl 1:S73-S78.
18. Rockey DC. Antifibrotic therapy in chronic liver disease. Clin Gastroenterol Hepatol, 2005, 3(2):95-107.
19. 李婧婷, 汪晖, 廖长秀. 肝星状细胞激活的分子机制与抗肝纤维化的治疗. 武汉大学学报, 2007, 28(2):256-261.
20. Li J, Qiu SJ, She WM, et al. Significance of the balance between regulatory T (Treg) and T helper 17(Th17) cells during hepatitis B virus related liver fibrosis. PLoS One, 2012, 7(6):e39307.
21. Florian W, Guido H, Ralf W, et al. Ito cells are liver-resident antigen-presenting cells for activating T cell responses. Immunity, 2007, 26(1):117-129.
22. Weiskirchen R, Tacke F. Cellular and molecular functions of hepatic stellate cells in inflammatory responses and liver immunology. Hepatobiliary Surg Nutr, 2014, 3(6):344-363.
23. Yu MC, Chen CH, Liang X, et al. Inhibition of T-cell responses by hepatic stellate cells via B7-H1-mediated T-cell apoptosis in mice. Hepatology, 2004, 40(6):1312-1321.
24. Chen CH, Liang-Mou K, Chang Y, et al. In vivo immune modulatory activity of hepatic stellate cells in mice. Hepatology, 2006, 44(5):1171-1181.
25. Guoping J, Horng-Ren Y, Lianfu W, et al. Hepatic stellate cells preferentially expand allogeneic CD4+ CD25+ FoxP3+ regulatory T cells in an IL-2-dependent manner. Transplantation, 2009, 86(11):1492-1502.
26. Yang HR, Hong-Shuie C, Gu X, et al. Mechanistic insights into immunomodulation by hepatic stellate cells in mice:a critical role of interferon-gamma signaling. Hepatology, 2009, 50(6):1981-1991.
27. Bastian H, Ormandy LA, Matthias B, et al. A new population of myeloid-derived suppressor cells in hepatocellular carcinoma patients induces CD4(+)CD25(+)Foxp3(+) T cells. Gastroenterology, 2008, 135(1):234-243.
28. Chen CH, Yu MC, Lu L, et al.215 Hepatic stellate cells regulate immune responses by induction of activated T cells apoptosis. Hepatology, 2003, 38(38):260.

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Effect of Echinococcus Cyst Fluid on Proliferation and Cell Cycle Progression of Rat Hepatic Stellate Cell

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