Significance of Glucocorticoid for Rat Liver Transplantation Model_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 LIUBo-qiang ,  RANJiang-hua , LIWen-kai , ZHENGKe-pu

Department of Hepatobiliary Surgery, Affiliated Ganmei Hospital of Kunming Medical University(The First People's Hospital of Kunming), Kunming 650011, Yunnan Province, China;

Corresponding author：

RANJiang-hua, Email: rjh2u@163.com

Keywords：

Rat; Liver transplantation; Glucocorticoid

DOI：

10.7507/1007-9424.20160148

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To explore significance of glucocorticoid for rat liver transplantation model. Methods Two hundred rats were randomly divided into experimental group and control group. The experimental group rats were injected with sulfate atropine resulotion 0.1 mg/kg, cephazolin-Na 0.3 g/kg and hydrocortisone 5 mg/kg while the control group rats were injected with sulfate atropine resulotion 0.1 mg/kg, cephazolin-Na 0.3 g/kg and equal volume of normal saline with glucocorticoid at 30 min before operation. The donor surgery time, repairing liver time, recipient surgery time, anhepatic phase, and 1-day, 3-day and 7-day survival rates were compared between these two groups. Results The donor surgery time, repairing liver time, recipient surgery time, and anhepatic phase had no significant differences between the experimental group and control group (P>0.05), while the 1-day, 3-day and 7-day survival rates of the experimental group were significantly higher than those of the control group [96% (48/50) versus 80% (40/50), P<0.05; 92% (46/50) versus 72% (36/50), P<0.05; 90% (45/50) versus 54% (27/50), P<0.05]. Conclusion Usage of glucocorticoid might contribute to improve survival rate of rat liver transplantation model.

Citation： LIUBo-qiang, RANJiang-hua, LIWen-kai, ZHENGKe-pu. Significance of Glucocorticoid for Rat Liver Transplantation Model. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(5): 552-555. doi: 10.7507/1007-9424.20160148 Copy

1.	?Kamada N, Calne RY. A surgical experience with five hundred thirty liver transplants in the rat. Surgery, 1983, 93(1 Pt 1):64-69.
2.	李立. 大鼠肝移植图谱. 北京:人民卫生出版社, 2012:1-46.
3.	刘剑, 李立, 张升宁, 等. 大鼠原位肝移植术后早期死亡的原因分析及处理策略. 中国普外基础与临床杂志, 2014, 21(12):1524-1530.
4.	姚坤厚, 高毅, 潘明新, 等. 大鼠原位肝移植急性排斥模型的建立. 实用医学杂志, 2008, 24(8):1304-1306.
5.	Tan F, Chen Z, Zhao Y, et al. Novel technique for suprahepatic vena cava reconstruction in rat orthotopic liver transplantation. Microsurgery, 2005, 25(7):556-560.
6.	Matevossian E, Doll D, Hüser N, et al. Liver transplantation in the rat:single-center experience with technique, long-term survival, and functional and histologic findings.Transplant Proc, 2009, 41(6):2631-2636.
7.	Kamada N, Calne RY. Orthotopic liver transplantation in the rat:Technique using cuff for portal vein anastomosis and biliary drainage. Transplantation, 1979, 28(1):47-50.
8.	Miyata M, Fischer H, Fuhs M, et al. A simple method for orthotopic liver transplantation in the rat:Cuff technique for three vascular anastomoses. Transplantation, 1980, 30(5):335-338.
9.	Inoue S, Tahara K, Shimizu H, et al. Rat liver transplantation for total vascular reconstruction, using a suture method. Microsurgery, 2003, 23(5):470-475.
10.	李镜锋, 丁焕新, 白建华, 等. 无心跳供肝大鼠肝移植模型的手术操作技巧探讨. 中国普外基础与临床杂志, 2010, 17(8):785-788.
11.	刘静, 李江, 张升宁, 等. 改良法构建大鼠减体积肝移植模型. 中国组织工程研究与临床康复, 2010, 14(18):3252-3257.
12.	李军, 窦科峰, 张洪涛, 等. 供肝快速切取法大鼠原位肝移植模型的建立. 中国普通外科杂志, 2006, 15(7):500-503.
13.	史源, 蒋安, 张勇, 等. 一种犬肝移植大血管快速重建的新方法. 中国修复重建外科杂志, 2007, 21(4):420-423.
14.	李萌, 潘崎, 程颖, 等. 褪黑素对大鼠移植肝组织GRP-78及CHOP表达的影响. 器官移植, 2015, 6(4):262-267.
15.	任秋平, 罗艳丽. 肝移植受者延续性护理需求及相关因素分析. 华西医学, 2015, 30(9):1720-1723.
16.	Grogan TA. Liver transplantation:issues and nursing care requirements. Crit Care Nurs Clin North, 2011, 23(3):443-456.
17.	Bulatao IG, Heckman MG, Rawal B, et al. Avoiding stay in the intensive care unit after liver transplantation:a score to assign location of care. Am J Transplant, 2014, 14(9):2088-2096.
18.	俞栋栋, 周琳, 郑树森. 肝移植临床可操作性耐受的研究现状及展望. 中华移植杂志:电子版, 2013, 7(2):103-107.
19.	夏瑾, 夏洋, 龚建平. 肝移植免疫抑制治疗临床应用进展. 中华移植杂志:电子版, 2013, 7(3):162-167.
20.	Zitta S, Schaffellner S, Gutschi J, et al. The effect of mammalian target of rapamycin versus calcineurin inhibitor-based immunosuppression on measured versus estimated glomerular filtration rate after orthotopic liver transplantation. Transplantation, 2015, 99(6):1250-1256.
21.	Charlton MR. Improving long-term outcomes after liver transplantation. Clin Liver Dis, 2014, 18(3):717-730.
22.	潘颖, 杨劼, 刘国强, 等. 肝移植患者用药分析. 河北医药, 2015, 37(8):1244-1246.
23.	Mouloudi E, Massa E, Papadopoulos S, et al. Bloodstream infections caused by carbapenemase-producing Klebsiella pneumoniae among intensive care unit patients after orthotopic liver transplantation:risk factors for infection and impact of resistance on outcomes. Transplant Proc, 2014, 46(9):3216-3218.
24.	Kim SI. Bacterial infection after liver transplantation. World J Gastroenterol, 2014, 20(20):6211-6220.
25.	Largiadèr F. Immunosuppression during clinical and experimental organ transplantation. Naturwissenschaften, 1968, 55(9):429-434.
26.	Åberg F. Role of budesonide in liver transplantation. Transpl Immunol, 2014, 30(4):178-179.
27.	石炳毅,朱志军,朱晓峰. 他克莫司在临床肝移植中的应用指南. 实用器官移植电子杂志, 2015, 31(3):696-698.
28.	魏晓晨, 朱立勤, 王春革. 激素对肝移植患者术后感染影响的Meta分析. 中国医院药学杂志, 2014, 34(9):735-742.
29.	李锐锋. 糖皮质激素在阻止急性肝衰竭发生中的作用与机制研究. 第三军医大学, 2014:14-19.
30.	徐建国, 江伟. 肾上腺糖皮质激素在围术期应用的专家共识. 临床麻醉学杂志, 2013, 29(2):200-204.

1. ?Kamada N, Calne RY. A surgical experience with five hundred thirty liver transplants in the rat. Surgery, 1983, 93(1 Pt 1):64-69.
2. 李立. 大鼠肝移植图谱. 北京:人民卫生出版社, 2012:1-46.
3. 刘剑, 李立, 张升宁, 等. 大鼠原位肝移植术后早期死亡的原因分析及处理策略. 中国普外基础与临床杂志, 2014, 21(12):1524-1530.
4. 姚坤厚, 高毅, 潘明新, 等. 大鼠原位肝移植急性排斥模型的建立. 实用医学杂志, 2008, 24(8):1304-1306.
5. Tan F, Chen Z, Zhao Y, et al. Novel technique for suprahepatic vena cava reconstruction in rat orthotopic liver transplantation. Microsurgery, 2005, 25(7):556-560.
6. Matevossian E, Doll D, Hüser N, et al. Liver transplantation in the rat:single-center experience with technique, long-term survival, and functional and histologic findings.Transplant Proc, 2009, 41(6):2631-2636.
7. Kamada N, Calne RY. Orthotopic liver transplantation in the rat:Technique using cuff for portal vein anastomosis and biliary drainage. Transplantation, 1979, 28(1):47-50.
8. Miyata M, Fischer H, Fuhs M, et al. A simple method for orthotopic liver transplantation in the rat:Cuff technique for three vascular anastomoses. Transplantation, 1980, 30(5):335-338.
9. Inoue S, Tahara K, Shimizu H, et al. Rat liver transplantation for total vascular reconstruction, using a suture method. Microsurgery, 2003, 23(5):470-475.
10. 李镜锋, 丁焕新, 白建华, 等. 无心跳供肝大鼠肝移植模型的手术操作技巧探讨. 中国普外基础与临床杂志, 2010, 17(8):785-788.
11. 刘静, 李江, 张升宁, 等. 改良法构建大鼠减体积肝移植模型. 中国组织工程研究与临床康复, 2010, 14(18):3252-3257.
12. 李军, 窦科峰, 张洪涛, 等. 供肝快速切取法大鼠原位肝移植模型的建立. 中国普通外科杂志, 2006, 15(7):500-503.
13. 史源, 蒋安, 张勇, 等. 一种犬肝移植大血管快速重建的新方法. 中国修复重建外科杂志, 2007, 21(4):420-423.
14. 李萌, 潘崎, 程颖, 等. 褪黑素对大鼠移植肝组织GRP-78及CHOP表达的影响. 器官移植, 2015, 6(4):262-267.
15. 任秋平, 罗艳丽. 肝移植受者延续性护理需求及相关因素分析. 华西医学, 2015, 30(9):1720-1723.
16. Grogan TA. Liver transplantation:issues and nursing care requirements. Crit Care Nurs Clin North, 2011, 23(3):443-456.
17. Bulatao IG, Heckman MG, Rawal B, et al. Avoiding stay in the intensive care unit after liver transplantation:a score to assign location of care. Am J Transplant, 2014, 14(9):2088-2096.
18. 俞栋栋, 周琳, 郑树森. 肝移植临床可操作性耐受的研究现状及展望. 中华移植杂志:电子版, 2013, 7(2):103-107.
19. 夏瑾, 夏洋, 龚建平. 肝移植免疫抑制治疗临床应用进展. 中华移植杂志:电子版, 2013, 7(3):162-167.
20. Zitta S, Schaffellner S, Gutschi J, et al. The effect of mammalian target of rapamycin versus calcineurin inhibitor-based immunosuppression on measured versus estimated glomerular filtration rate after orthotopic liver transplantation. Transplantation, 2015, 99(6):1250-1256.
21. Charlton MR. Improving long-term outcomes after liver transplantation. Clin Liver Dis, 2014, 18(3):717-730.
22. 潘颖, 杨劼, 刘国强, 等. 肝移植患者用药分析. 河北医药, 2015, 37(8):1244-1246.
23. Mouloudi E, Massa E, Papadopoulos S, et al. Bloodstream infections caused by carbapenemase-producing Klebsiella pneumoniae among intensive care unit patients after orthotopic liver transplantation:risk factors for infection and impact of resistance on outcomes. Transplant Proc, 2014, 46(9):3216-3218.
24. Kim SI. Bacterial infection after liver transplantation. World J Gastroenterol, 2014, 20(20):6211-6220.
25. Largiadèr F. Immunosuppression during clinical and experimental organ transplantation. Naturwissenschaften, 1968, 55(9):429-434.
26. Åberg F. Role of budesonide in liver transplantation. Transpl Immunol, 2014, 30(4):178-179.
27. 石炳毅,朱志军,朱晓峰. 他克莫司在临床肝移植中的应用指南. 实用器官移植电子杂志, 2015, 31(3):696-698.
28. 魏晓晨, 朱立勤, 王春革. 激素对肝移植患者术后感染影响的Meta分析. 中国医院药学杂志, 2014, 34(9):735-742.
29. 李锐锋. 糖皮质激素在阻止急性肝衰竭发生中的作用与机制研究. 第三军医大学, 2014:14-19.
30. 徐建国, 江伟. 肾上腺糖皮质激素在围术期应用的专家共识. 临床麻醉学杂志, 2013, 29(2):200-204.

Previous Article
Progress of Clinical Treatment of Hepatic Alveolar Echinococcosis
Next Article
Expressions of EGFR, BRAF, and K-Ras Genes in Colorectal Carcinoma Tissues and Their Clinical Significances

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Significance of Glucocorticoid for Rat Liver Transplantation Model

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content