Effect of Over-Expression MiR-92a on Clinicopathological Features and Prognosis of Patients with Colorectal Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 LIJian-shui ^1,3 , LILi-fa ^2,3 , ZHANGJing-xiao ³ , CHENXiao-bo ² , WUbin ¹ , LUOXia ⁴ , YANGSong ⁴

1. Department of Hepatobiliary Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, Sichuan Province, China;
2. Gastrointestinal of Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, Sichuan Province, China;
3. Institute of Hepato-Biliary-Pancreas and Intestinal Disease, North Sichuan Medical College, Nanchong 637000, Sichuan Province, China;
4. Department of Clinical Medicine, North Sichuan Medical College, Nanchong 637000, Sichuan Province, China;

Corresponding author：

LIJian-shui, Email: ljs2005doctor@126.com

Keywords：

MicroRNA-92a; Colorectal cancer; Phosphatase and tensin homologue; Prognosis

DOI：

10.7507/1007-9424.20160220

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the impact and mechanism of over-expression microRNA-92a (miR-92a) in clinicopathologic feature and prognosis of patients with colorectal cancer (CRC). Methods The expression levels of miR-92a and phosphatase and tensin homologue (PTEN) gene in 108 cases of colorectal cancer tissues were detected by using real-time fluorescent quantitative PCR (RT-PCR), and they were categorized as low or high in relation to the median value. Then the association between different levels of miR-92a gene expression and clinicopathologic feature and prognosis of CRC patients were evaluated. Moreover, the relationship between the expressions of miR-92a and PTEN gene were analyzed. Results High expression of miR-92a not only associated with lymph node metastasis (χ²=8.045, P=0.007), distant metastasis (χ²=5.708, P=0.030), and TNM staging (χ²=6.366, P=0.019) of CRC patients, but also related to the down-regulated of PTEN gene expression (χ²=21.333, P < 0.001). However, up-regulated miR-92a expression was negatively correlated with age, gender, tumor size, tumor location, depth of invasion and tumor differentiation. In addition, Kaplan-Meier survival curves showed that over-expression of miR-92a and low-expression of PTEN gene could potentially predict poor overall survival of CRC patients. Conclusion The high expression of miR-92a can lead to a poor clinical prognosis of CRC patients through decreasing the expression level of PTEN gene.

Citation： LIJian-shui, LILi-fa, ZHANGJing-xiao, CHENXiao-bo, WUbin, LUOXia, YANGSong. Effect of Over-Expression MiR-92a on Clinicopathological Features and Prognosis of Patients with Colorectal Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(7): 851-854. doi: 10.7507/1007-9424.20160220 Copy

1.	Jemal A, Bray F, Center MM, et al. Global cancer statistics. CA Cancer J Clin, 2011, 61(2): 69-90.
2.	王飞, 董国礼.结直肠癌的影像学研究进展.川北医学院学报, 2014, 29(1): 107-112.
3.	王宁, 孙婷婷, 郑荣寿, 等.中国2009年结直肠癌发病和死亡资料分析.中国肿瘤, 2013, 22(7): 515-520.
4.	李利发, 周彤, 刘佳嘉, 等. microRNA-106b-25簇在结直肠癌中的作用研究进展.中华临床医师杂志:电子版, 2015, 9(12): 2384-2387.
5.	Muhammad S, Kaur K, Huang R, et al. MicroRNAs in colorectal cancer: role in metastasis and clinical perspectives. World J Gastroen-terol, 2014, 20(45): 17011-17019.
6.	Ji D, Chen Z, Li M, et al. MicroRNA-181a promotes tumor growth and liver metastasis in colorectal cancer by targeting the tumor suppre ssor WIF-1. Mol Cancer, 2014, 13: 86.
7.	Zhang GJ, Zhou H, Xiao HX, et al. MiR-378 is an independent prognostic factor and inhibits cell growth and invasion in colorectal cancer. BMC Cancer, 2014, 14: 109.
8.	Zhang GJ, Li Y, Zhou H, et al. miR20a is an independent prognostic factor in colorectal cancer and is involved in cell metastasis. Mol Med Rep, 2014, 10(1): 283-291.
9.	Olive V, Jiang I, He L. mir-17-92, a cluster of miRNAs in the midst of the cancer network. Int J Biochem Cell Biol, 2010, 42(8): 1348-1354.
10.	Ren P, Gong F, Zhang Y, et al. MicroRNA-92a promotes growth, metastasis, and chemoresistance in non-small cell lung cancer cells by targeting PTEN. Tumour Biol, 2016, 37(3): 3215-3225.
11.	Yang W, Dou C, Wang Y, et al. MicroRNA-92a contributes to tumor growth of human hepatocellular carcinoma by targeting FBXW7. Oncol Rep, 2015, 34(5): 2576-2584.
12.	Chen ZL, Zhao XH, Wang JW, et al. microRNA-92a promotes lymph node metastasis of human esophageal squamous cell carcinoma via E-cadherin. J Biol Chem, 2011, 286(12): 10725-10734.
13.	Si H, Sun X, Chen Y et al. Circulating microRNA-92a and micro-RNA-21 as novel minimally invasive biomarkers for primary breast cancer. J Cancer Res Clin Oncol, 2013, 139(2): 223-229.
14.	Nishida N, Nagahara M, Sato T, et al. Microarray analysis of colore-ctal cancer stromal tissue reveals upregulation of two oncogenic miRNA clusters. Clin Cancer Res, 2012, 18(11): 3054-3070.
15.	Yamada N, Nakagawa Y, Tsujimura N, et al. Role of intracellular and extracellular microRNA-92a in colorectal cancer. Transl Oncol, 2013, 6(4): 482-492.
16.	Huang Z, Huang D, Ni S, et al. Plasma microRNAs are promising novel biomarkers for early detection of colorectal cancer. Int J Cancer, 2010, 127(1): 118-126.
17.	Liu GH, Zhou ZG, Chen R, et al. Serum miR-21 and miR-92a as biomarkers in the diagnosis and prognosis of colorectal cancer. Tumour Biol, 2013, 34(4): 2175-2181.
18.	Ke TW, Wei PL, Yeh KT, et al. MiR-92a promotes cell metastasis of colorectal cancer through PTEN-mediated PI3K/AKT pathway. Ann Surg Oncol, 2015, 22(8): 2649-2655.
19.	Zhou T, Zhang G, Liu Z, et al. Over expression of miR-92a correlates with tumor metastasis and poor prognosis in patients with colorectal cancer. Int J Colorectal Dis, 2013, 28(1): 19-24.
20.	Schee K, Boye K, Abrahamsen TW, et al. Clinical relevance of microRNA miR-21, miR-31, miR-92a, miR-101, miR-106a and miR-145 in colorectal cancer. BMC Cancer, 2012, 12: 505.
21.	Zhang G, Zhou H, Xiao H, et al. MicroRNA-92a functions as an oncogene in colorectal cancer by targeting PTEN. Dig Dis Sci, 2014, 59(1): 98-107.

1. Jemal A, Bray F, Center MM, et al. Global cancer statistics. CA Cancer J Clin, 2011, 61(2): 69-90.
2. 王飞, 董国礼.结直肠癌的影像学研究进展.川北医学院学报, 2014, 29(1): 107-112.
3. 王宁, 孙婷婷, 郑荣寿, 等.中国2009年结直肠癌发病和死亡资料分析.中国肿瘤, 2013, 22(7): 515-520.
4. 李利发, 周彤, 刘佳嘉, 等. microRNA-106b-25簇在结直肠癌中的作用研究进展.中华临床医师杂志:电子版, 2015, 9(12): 2384-2387.
5. Muhammad S, Kaur K, Huang R, et al. MicroRNAs in colorectal cancer: role in metastasis and clinical perspectives. World J Gastroen-terol, 2014, 20(45): 17011-17019.
6. Ji D, Chen Z, Li M, et al. MicroRNA-181a promotes tumor growth and liver metastasis in colorectal cancer by targeting the tumor suppre ssor WIF-1. Mol Cancer, 2014, 13: 86.
7. Zhang GJ, Zhou H, Xiao HX, et al. MiR-378 is an independent prognostic factor and inhibits cell growth and invasion in colorectal cancer. BMC Cancer, 2014, 14: 109.
8. Zhang GJ, Li Y, Zhou H, et al. miR20a is an independent prognostic factor in colorectal cancer and is involved in cell metastasis. Mol Med Rep, 2014, 10(1): 283-291.
9. Olive V, Jiang I, He L. mir-17-92, a cluster of miRNAs in the midst of the cancer network. Int J Biochem Cell Biol, 2010, 42(8): 1348-1354.
10. Ren P, Gong F, Zhang Y, et al. MicroRNA-92a promotes growth, metastasis, and chemoresistance in non-small cell lung cancer cells by targeting PTEN. Tumour Biol, 2016, 37(3): 3215-3225.
11. Yang W, Dou C, Wang Y, et al. MicroRNA-92a contributes to tumor growth of human hepatocellular carcinoma by targeting FBXW7. Oncol Rep, 2015, 34(5): 2576-2584.
12. Chen ZL, Zhao XH, Wang JW, et al. microRNA-92a promotes lymph node metastasis of human esophageal squamous cell carcinoma via E-cadherin. J Biol Chem, 2011, 286(12): 10725-10734.
13. Si H, Sun X, Chen Y et al. Circulating microRNA-92a and micro-RNA-21 as novel minimally invasive biomarkers for primary breast cancer. J Cancer Res Clin Oncol, 2013, 139(2): 223-229.
14. Nishida N, Nagahara M, Sato T, et al. Microarray analysis of colore-ctal cancer stromal tissue reveals upregulation of two oncogenic miRNA clusters. Clin Cancer Res, 2012, 18(11): 3054-3070.
15. Yamada N, Nakagawa Y, Tsujimura N, et al. Role of intracellular and extracellular microRNA-92a in colorectal cancer. Transl Oncol, 2013, 6(4): 482-492.
16. Huang Z, Huang D, Ni S, et al. Plasma microRNAs are promising novel biomarkers for early detection of colorectal cancer. Int J Cancer, 2010, 127(1): 118-126.
17. Liu GH, Zhou ZG, Chen R, et al. Serum miR-21 and miR-92a as biomarkers in the diagnosis and prognosis of colorectal cancer. Tumour Biol, 2013, 34(4): 2175-2181.
18. Ke TW, Wei PL, Yeh KT, et al. MiR-92a promotes cell metastasis of colorectal cancer through PTEN-mediated PI3K/AKT pathway. Ann Surg Oncol, 2015, 22(8): 2649-2655.
19. Zhou T, Zhang G, Liu Z, et al. Over expression of miR-92a correlates with tumor metastasis and poor prognosis in patients with colorectal cancer. Int J Colorectal Dis, 2013, 28(1): 19-24.
20. Schee K, Boye K, Abrahamsen TW, et al. Clinical relevance of microRNA miR-21, miR-31, miR-92a, miR-101, miR-106a and miR-145 in colorectal cancer. BMC Cancer, 2012, 12: 505.
21. Zhang G, Zhou H, Xiao H, et al. MicroRNA-92a functions as an oncogene in colorectal cancer by targeting PTEN. Dig Dis Sci, 2014, 59(1): 98-107.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Over-Expression MiR-92a on Clinicopathological Features and Prognosis of Patients with Colorectal Cancer

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content