Acute pancreas induced by tissue factor expression which regulated by Toll-like receptor 4 pathway in Kupffer cell_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LI Fangping ^1,2 , WEN Jian ¹ , HU Yingchun ¹ , OU Zhibing ¹ , ZHU Rongtao ¹ ,  GONG Jianping ¹

1. Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, P. R. China;
2. Department of Surgery, People’s Hospital in Yubei Country of Chongqing City, Chongqing 401120, P. R. China;

Corresponding author：

GONG Jianping, Email: gongjianping11@126.com

Keywords：

acute pancreatitis; Kupffer cell; Toll-like receptor 4; tissue factor; tissue factor-bearing microparticle

DOI：

10.7507/1007-9424.201712084

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To verify tissue factor (TF)-bearing microparticle (TF-MP) could be released from Kupffer cells (KCs) stimulated by lipopolysaccharide (LPS) and TF controlled by Toll-like receptor 4 (TLR4) could induce acute pancreatitis. Methods After the acute pancreatitis model completed, the wild type C57/BL6 mouse (WT group) and the TLR4^-/- mouse (TLR4^-/- group) received intraperitioneal injections of 10 mg/kg LPS. The degree of pathological lesion and the TF expression were detected in the pancreas tissue. The TF and TLR4 protein and mRNA expressions in the KCs were detected at 6, 12, and 24 h after the last injection of LPS. The survival rates were campared in these estabilshed acute pancreatitis model mice. The TF and TLR4 protein and mRNA expressions in the KCs stimulated with LPS (300 μg/L) were also detected at 0, 15, 30, 60, and 120 min. The TF and TF-MP levels were detected in the supernatants of the KCs at these time point. Results The injury of the pancreas in the TLR4^-/- group was slighter than that in the WT group. The TF proteins in the liver and pancreas tissues of the TLR4^-/- group were significantly lower than those of the WT group (P<0.05). The survival rate of the TLR4^-/- group was significantly higher than that of the WT group under the situation of the acute pancreatitis (P<0.05). The TLR4 and TF protien and mRNA expressions of the KCs were significantly decreased in the TLR4^-/- group as compared with the WT group at 30, 60, and 120 min (P<0.05). The levels of TF and TF-MP in the supernatant of the TLR4^-/- group were significantly lower than those of the WT group at 30, 60, and 120 min (P<0.05). Conclusion Acute pancretitis can be induced by TF and TF-MP expressions in KCs which could be regulated by TLR4 pathway.

Citation： LI Fangping, WEN Jian, HU Yingchun, OU Zhibing, ZHU Rongtao, GONG Jianping. Acute pancreas induced by tissue factor expression which regulated by Toll-like receptor 4 pathway in Kupffer cell. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2018, 25(7): 784-791. doi: 10.7507/1007-9424.201712084 Copy

1.	许多强. 重症急性胰腺炎临床路径探讨. 医药前沿, 2017, 7(8): 50-51.
2.	幸定培, 龚建平, 冯虎翼, 等. 枯否细胞通过组织因子对大鼠急性胰腺炎凝血系统变化的影响. 重庆医科大学学报, 2015, 40(3): 329-334.
3.	Cimmino G, Ciccarelli G, Golino P. Role of tissue factor in the coagulation network. Semin Thromb Hemost, 2015, 41(7): 708-717.
4.	Li P, Li M, He K, et al. The effects of Twist-2 on liver endotoxin tolerance induced by a low dose of lipopolysaccharide. Inflammation, 2014, 37(1): 55-64.
5.	Li PZ, Li JZ, Li M, et al. An efficient method to isolate and culture mouse Kupffer cells. Immunol Lett, 2014, 158(1-2): 52-56.
6.	Zhang XP, Li ZJ, Zhang J. Inflammatory mediators and microcirculatory disturbance in acute pancreatitis. Hepatobiliary Pancreat Dis Int, 2009, 8(4): 351-357.
7.	Andersson E, Axelsson J, Eckerwall G, et al. Tissue factor in predicted severe acute pancreatitis. World J Gastroenterol, 2010, 16(48): 6128-6134.
8.	Camera M, Toschi V, Brambilla M, et al. The role of tissue factor in atherothrombosis and coronary artery disease: insights into platelet tissue factor. Semin Thromb Hemost, 2015, 41(7): 737-746.
9.	Zhang J, Yang J, Xu X, et al. The influence of genetic polymorphisms in TLR4 and TIRAP, and their expression levels in peripheral blood, on susceptibility to sepsis. Exp Ther Med, 2016, 11(1): 131-139.
10.	Siemiatkowski A, Wereszczynska-Siemiatkowska U, Mroczko B, et al. Circulating endothelial mediators in human pancreatitis-associated lung injury. Eur J Gastroenterol Hepatol, 2015, 27(6): 728-734.
11.	Meher S, Mishra TS, Sasmal PK, et al. Role of biomarkers in diagnosis and prognostic evaluation of acute pancreatitis. J Biomark, 2015, 2015: 519534.
12.	Eisenreich A, Bolbrinker J, Leppert U. Tissue factor: a conventional or alternative target in cancer therapy. Clin Chem, 2016, 62(4): 563-570.
13.	Wang B, Xiong S, Hua Q, et al. Tissue factor is strongly expressed in pericarcinomatous tissue in patients with laryngeal carcinoma. Int J Clin Exp Pathol, 2015, 8(10): 13719-13724.
14.	Morgan CE, Dombrowski AW, Rubert Pérez CM, et al. Tissue-factor targeted peptide amphiphile nanofibers as an injectable therapy to control hemorrhage. ACS Nano, 2016, 10(1): 899-909.
15.	Chiva-Blanch G, Laake K, Myhre P, et al. Platelet-, monocyte-derived and tissue factor-carrying circulating microparticles are related to acute myocardial infarction severity. PLoS One, 2017, 12(2): e0172558.
16.	Witkowski M, Landmesser U, Rauch U. Tissue factor as a link between inflammation and coagulation. Trends Cardiovasc Med, 2016, 26(4): 297-303.
17.	Santilli F, Marchisio M, Lanuti P, et al. Microparticles as new markers of cardiovascular risk in diabetes and beyond. Thromb Haemost, 2016, 116(2): 220-234.
18.	Christersson C, Thulin Å, Siegbahn A. Microparticles during long-term follow-up after acute myocardial infarction. Association to atherosclerotic burden and risk of cardiovascular events. Thromb Haemost, 2017, 117(8): 1571-1581.
19.	Hisada Y, Alexander W, Kasthuri R, et al. Measurement of microparticle tissue factor activity in clinical samples: A summary of two tissue factor-dependent FXa generation assays. Thromb Res, 2016, 139: 90-97.
20.	Bala S, Csak T, Kodys K, et al. Alcohol-induced miR-155 and HDAC11 inhibit negative regulators of the TLR4 pathway and lead to increased LPS responsiveness of Kupffer cells in alcoholic liver disease. J Leukoc Biol, 2017, 102(2): 487-498.
21.	Peng Y, Sigua CA, Rideout D, et al. Deletion of toll-like receptor-4 downregulates protein kinase C-zeta and attenuates liver injury in experimental pancreatitis. Surgery, 2008, 143(5): 679-685.
22.	Kim JE, Yoo HJ, Gu JY, et al. Histones induce the procoagulant phenotype of endothelial cells through tissue factor up-regulation and thrombomodulin down-regulation. PLoS One, 2016, 11(6): e0156763.
23.	Siemiatkowski A, Wereszczynska-Siemiatkowska U, Mroczko B, et al. Circulating endothelial mediators in human pancreatitis-associated lung injury. Eur J Gastroenterol Hepatol, 2015, 27(6): 728-734.
24.	Pan LF, Yu L, Wang LM, et al. Augmenter of liver regeneration (ALR) regulates acute pancreatitis via inhibiting HMGB1/TLR4/NF-κB signaling pathway. Am J Transl Res, 2018, 10(2): 402-410.
25.	Wang B, Xu XB, Jin XX, et al. Effects of ω-3 fatty acids on Toll-like receptor 4 and nuclear factor κB p56 in the pancreas of rats with severe acute pancreatitis. Pancreas, 2017, 46(10): 1267-1274.
26.	Franco-Avilés L, Hernández-Rocha FI, Mercado U, et al. Segmental portal hypertension with splenic vein thrombosis caused by pancreatitis. Rev Med Inst Mex Seguro Soc, 2017, 55(6): 788-790.
27.	Fei Y, Hu J, Gao K, et al. Risk prediction for portal vein thrombosis in acute pancreatitis using radial basis function. Ann Vasc Surg, 2018, 47: 78-84.

1. 许多强. 重症急性胰腺炎临床路径探讨. 医药前沿, 2017, 7(8): 50-51.
2. 幸定培, 龚建平, 冯虎翼, 等. 枯否细胞通过组织因子对大鼠急性胰腺炎凝血系统变化的影响. 重庆医科大学学报, 2015, 40(3): 329-334.
3. Cimmino G, Ciccarelli G, Golino P. Role of tissue factor in the coagulation network. Semin Thromb Hemost, 2015, 41(7): 708-717.
4. Li P, Li M, He K, et al. The effects of Twist-2 on liver endotoxin tolerance induced by a low dose of lipopolysaccharide. Inflammation, 2014, 37(1): 55-64.
5. Li PZ, Li JZ, Li M, et al. An efficient method to isolate and culture mouse Kupffer cells. Immunol Lett, 2014, 158(1-2): 52-56.
6. Zhang XP, Li ZJ, Zhang J. Inflammatory mediators and microcirculatory disturbance in acute pancreatitis. Hepatobiliary Pancreat Dis Int, 2009, 8(4): 351-357.
7. Andersson E, Axelsson J, Eckerwall G, et al. Tissue factor in predicted severe acute pancreatitis. World J Gastroenterol, 2010, 16(48): 6128-6134.
8. Camera M, Toschi V, Brambilla M, et al. The role of tissue factor in atherothrombosis and coronary artery disease: insights into platelet tissue factor. Semin Thromb Hemost, 2015, 41(7): 737-746.
9. Zhang J, Yang J, Xu X, et al. The influence of genetic polymorphisms in TLR4 and TIRAP, and their expression levels in peripheral blood, on susceptibility to sepsis. Exp Ther Med, 2016, 11(1): 131-139.
10. Siemiatkowski A, Wereszczynska-Siemiatkowska U, Mroczko B, et al. Circulating endothelial mediators in human pancreatitis-associated lung injury. Eur J Gastroenterol Hepatol, 2015, 27(6): 728-734.
11. Meher S, Mishra TS, Sasmal PK, et al. Role of biomarkers in diagnosis and prognostic evaluation of acute pancreatitis. J Biomark, 2015, 2015: 519534.
12. Eisenreich A, Bolbrinker J, Leppert U. Tissue factor: a conventional or alternative target in cancer therapy. Clin Chem, 2016, 62(4): 563-570.
13. Wang B, Xiong S, Hua Q, et al. Tissue factor is strongly expressed in pericarcinomatous tissue in patients with laryngeal carcinoma. Int J Clin Exp Pathol, 2015, 8(10): 13719-13724.
14. Morgan CE, Dombrowski AW, Rubert Pérez CM, et al. Tissue-factor targeted peptide amphiphile nanofibers as an injectable therapy to control hemorrhage. ACS Nano, 2016, 10(1): 899-909.
15. Chiva-Blanch G, Laake K, Myhre P, et al. Platelet-, monocyte-derived and tissue factor-carrying circulating microparticles are related to acute myocardial infarction severity. PLoS One, 2017, 12(2): e0172558.
16. Witkowski M, Landmesser U, Rauch U. Tissue factor as a link between inflammation and coagulation. Trends Cardiovasc Med, 2016, 26(4): 297-303.
17. Santilli F, Marchisio M, Lanuti P, et al. Microparticles as new markers of cardiovascular risk in diabetes and beyond. Thromb Haemost, 2016, 116(2): 220-234.
18. Christersson C, Thulin Å, Siegbahn A. Microparticles during long-term follow-up after acute myocardial infarction. Association to atherosclerotic burden and risk of cardiovascular events. Thromb Haemost, 2017, 117(8): 1571-1581.
19. Hisada Y, Alexander W, Kasthuri R, et al. Measurement of microparticle tissue factor activity in clinical samples: A summary of two tissue factor-dependent FXa generation assays. Thromb Res, 2016, 139: 90-97.
20. Bala S, Csak T, Kodys K, et al. Alcohol-induced miR-155 and HDAC11 inhibit negative regulators of the TLR4 pathway and lead to increased LPS responsiveness of Kupffer cells in alcoholic liver disease. J Leukoc Biol, 2017, 102(2): 487-498.
21. Peng Y, Sigua CA, Rideout D, et al. Deletion of toll-like receptor-4 downregulates protein kinase C-zeta and attenuates liver injury in experimental pancreatitis. Surgery, 2008, 143(5): 679-685.
22. Kim JE, Yoo HJ, Gu JY, et al. Histones induce the procoagulant phenotype of endothelial cells through tissue factor up-regulation and thrombomodulin down-regulation. PLoS One, 2016, 11(6): e0156763.
23. Siemiatkowski A, Wereszczynska-Siemiatkowska U, Mroczko B, et al. Circulating endothelial mediators in human pancreatitis-associated lung injury. Eur J Gastroenterol Hepatol, 2015, 27(6): 728-734.
24. Pan LF, Yu L, Wang LM, et al. Augmenter of liver regeneration (ALR) regulates acute pancreatitis via inhibiting HMGB1/TLR4/NF-κB signaling pathway. Am J Transl Res, 2018, 10(2): 402-410.
25. Wang B, Xu XB, Jin XX, et al. Effects of ω-3 fatty acids on Toll-like receptor 4 and nuclear factor κB p56 in the pancreas of rats with severe acute pancreatitis. Pancreas, 2017, 46(10): 1267-1274.
26. Franco-Avilés L, Hernández-Rocha FI, Mercado U, et al. Segmental portal hypertension with splenic vein thrombosis caused by pancreatitis. Rev Med Inst Mex Seguro Soc, 2017, 55(6): 788-790.
27. Fei Y, Hu J, Gao K, et al. Risk prediction for portal vein thrombosis in acute pancreatitis using radial basis function. Ann Vasc Surg, 2018, 47: 78-84.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Acute pancreas induced by tissue factor expression which regulated by Toll-like receptor 4 pathway in Kupffer cell

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content