The expression and clinical significance of miR-143-3p in gastric cancer tissues with bioinformatical analysis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

NIU Rong ¹ , WANG Zhuanxiong ² , BAI Yue ¹ , ZHAO Wentao ¹ , HU Yonghua ² , TIAN Yihong ² ,  LI Hailong ²

1. No.1 Department of Thoracic Surgery, Gansu Provincial Cancer Hospital, Lanzhou 730050, P. R. China;
2. School of Clinical Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, P. R. China;

Corresponding author：

LI Hailong, Email: gslz860931@163.com

Keywords：

gastric cancer; miR-143-3p; tumor molecular marker; bioinformatics analysis

DOI：

10.7507/1007-9424.201901023

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To observe the expressions of miR-143-3p in gastric cancer cells and gastric carcinoma tissues with its clinical significance, and to analyze the target genes with enriched pathway by using bioinformatics methods.Methods The expressions of miR-143-3p in different differentiation gastric cancer cells and normal gastric mucosa cell line, and the expressions in gastric cancer tissues and adjacent tissues were detected by real-time fluorescent quantitative PCR. In addition, OncomiR and YM500 databases were used to analyze the expression of miR-143-3p in gastric cancer tissues compared with adjacent tissues. Furthermore, the targets of miR-143-3p were predicted by using the software of miRecords website database, and at least three software-supported target genes were chosen to analyze the enriched the signal pathways in which the target gene was involved with DAVID 6.7 software.Results The expressions of miR-143-3p in the different differentiation degree of gastric cancer cells compared with normal gastric mucosa cell line were downregulated (P<0.001), and the expression of miR-143-3p in gastric cancer tissues compared with adjacent tissues was also downregulated (downregulated in 36 cases, upregulated in 18 cases, and no alteration in 4 cases). The expression of miR-143-3p in gastric cancer tissues was associated with lymph node metastasis and invasion depth (P<0.05). Bioinformatics analysis results showed that the target genes of miR-143-3p were enriched in 38 signaling pathways associated with cancer.Conclusion MiR-143-3p is a down-regulated molecular marker in gastric cancer and a potentially clinically related tumor suppressor gene, which may be involved in the cancerous phenotype in carcinogenesis and development of gastric cancer.

Citation： NIU Rong, WANG Zhuanxiong, BAI Yue, ZHAO Wentao, HU Yonghua, TIAN Yihong, LI Hailong. The expression and clinical significance of miR-143-3p in gastric cancer tissues with bioinformatical analysis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2019, 26(5): 538-544. doi: 10.7507/1007-9424.201901023 Copy

1.	Chen W, Zheng R, Baade PD, et al. Cancer statistics in China, 2015. CA Cancer J Clin, 2016, 66(2): 115-132.
2.	Obermannova R, Redova-Lojova M, Vychytilova-Faltejskova P, et al. Tumor expression of miR-10b, miR-21, miR-143 and miR-145 is related to clinicopathological features of gastric cancer in a central European population. Anticancer Res, 2018, 38(6): 3719-3724.
3.	Jiang X, Wang W, Yang Y, et al. Identification of circulating microRNA signatures as potential noninvasive biomarkers for prediction and prognosis of lymph node metastasis in gastric cancer. Oncotarget, 2017, 8(39): 65132-65142.
4.	Wang F, Liu J, Zou Y, et al. MicroRNA-143-3p, up-regulated in H. pylori-positive gastric cancer, suppresses tumor growth, migration and invasion by directly targeting AKT2. Oncotarget, 2017, 8(17): 28711-28724.
5.	邢晓芳, 李子禹. miR-143和miR-145在胃癌中的表达及功能研究. 中华胃肠外科杂志, 2015, 18(1): 50-53.
6.	Guo B, Li J, Liu L, et al. Dysregulation of miRNAs and their potential as biomarkers for the diagnosis of gastric cancer. Biomed Rep, 2013, 1(6): 907-912.
7.	李海龙, 宋耀辉, 陈兆峰, 等. miR-20a-5p/miR-20b-5p在胃癌细胞和组织中的表达及调控靶基因信号通路富集分析. 临床检验杂志, 2017, 35(11): 822-827.
8.	郗洪庆, 张珂诚, 卫勃, 等. 胃癌TNM分期第八版更新在临床诊断治疗中的意义和思考. 中华胃肠外科杂志, 2017, 20(2): 166-170.
9.	康博雄, 李海龙, 陈彻, 等. miR-106a-5p在胃癌细胞和胃癌组织中的表达及其调控靶基因信号通路富集分析. 中国普外基础与临床杂志, 2018, 25(8): 923-928.
10.	Li H, Xie S, Liu M, et al. The clinical significance of down-regulation of mir-124-3p, mir-146a-5p, mir-155-5p and mir-335-5p in gastric cancer tumorigenesis. Int J Oncol, 2014, 45(1): 197-208.
11.	Li X, Luo F, Li Q, et al. Identification of new aberrantly expressed miRNAs in intestinal-type gastric cancer and its clinical significance. Oncol Rep, 2011, 26(6): 1431-1439.
12.	Zhou LL, Dong JL, Huang G, et al. MicroRNA-143 inhibits cell growth by targeting ERK5 and MAP3K7 in breast cancer. Braz J Med Biol Res, 2017, 50(8): e5891.
13.	Jin YP, Hu YP, Wu XS, et al. miR-143-3p targeting of ITGA6 suppresses tumour growth and angiogenesis by downregulating PLGF expression via the PI3K/AKT pathway in gallbladder carcinoma. Cell Death Dis, 2018, 9(2): 182.
14.	Dong X, Lv B, Li Y, et al. MiR-143 regulates the proliferation and migration of osteosarcoma cells through targeting MAPK7. Arch Biochem Biophys, 2017, 630: 47-53.
15.	Sun X, Dai G, Yu L, et al. miR-143-3p inhibits the proliferation, migration and invasion in osteosarcoma by targeting FOSL2. Sci Rep, 2018, 8(1): 606.
16.	Shi H, Shen H, Xu J, et al. MiR-143-3p suppresses the progression of ovarian cancer. Am J Transl Res, 2018, 10(3): 866-874.
17.	Panza E, Ercolano G, De Cicco P, et al. MicroRNA-143-3p inhibits growth and invasiveness of melanoma cells by targeting cyclooxygenase-2 and inversely correlates with malignant melanoma progression. Biochem Pharmacol, 2018, 156: 52-59.
18.	Huang SJ, Zhu YF, Liu Z, et al. Study of miR-143 expression in stomach cancer. Oncol Lett, 2018, 16(4): 4367-4371.
19.	梁莹, 叶延程, 张文华, 等. 2008~2012年甘肃省武威市凉州区胃癌发病与死亡分析. 中国肿瘤, 2016, 25(7): 519-523.
20.	Lei C, Du F, Sun L, et al. miR-143 and miR-145 inhibit gastric cancer cell migration and metastasis by suppressing MYO6. Cell Death Dis, 2017, 8(10): e3101.
21.	Zhang Q, Feng Y, Liu P, et al. MiR-143 inhibits cell proliferation and invasion by targeting DNMT3A in gastric cancer. Tumour Biol, 2017, 39(7): 1010428317711312.
22.	Wu XL, Cheng B, Li PY, et al. MicroRNA-143 suppresses gastric cancer cell growth and induces apoptosis by targeting COX-2. World J Gastroenterol, 2013, 19(43): 7758-7765.
23.	Guoping M, Ran L, Yanru Q. miR-143 inhibits cell proliferation of gastric cancer cells through targeting GATA6. Oncol Res, 2018, 26(7): 1023-1029.
24.	Zhuang M, Shi Q, Zhang X, et al. Involvement of miR-143 in cisplatin resistance of gastric cancer cells via targeting IGF1R and BCL2. Tumour Biol, 2015, 36(4): 2737-2745.
25.	Xu B, Niu X, Zhang X, et al. miR-143 decreases prostate cancer cells proliferation and migration and enhances their sensitivity to docetaxel through suppression of KRAS. Mol Cell Biochem, 2011, 350(1-2): 207-213.

1. Chen W, Zheng R, Baade PD, et al. Cancer statistics in China, 2015. CA Cancer J Clin, 2016, 66(2): 115-132.
2. Obermannova R, Redova-Lojova M, Vychytilova-Faltejskova P, et al. Tumor expression of miR-10b, miR-21, miR-143 and miR-145 is related to clinicopathological features of gastric cancer in a central European population. Anticancer Res, 2018, 38(6): 3719-3724.
3. Jiang X, Wang W, Yang Y, et al. Identification of circulating microRNA signatures as potential noninvasive biomarkers for prediction and prognosis of lymph node metastasis in gastric cancer. Oncotarget, 2017, 8(39): 65132-65142.
4. Wang F, Liu J, Zou Y, et al. MicroRNA-143-3p, up-regulated in H. pylori-positive gastric cancer, suppresses tumor growth, migration and invasion by directly targeting AKT2. Oncotarget, 2017, 8(17): 28711-28724.
5. 邢晓芳, 李子禹. miR-143和miR-145在胃癌中的表达及功能研究. 中华胃肠外科杂志, 2015, 18(1): 50-53.
6. Guo B, Li J, Liu L, et al. Dysregulation of miRNAs and their potential as biomarkers for the diagnosis of gastric cancer. Biomed Rep, 2013, 1(6): 907-912.
7. 李海龙, 宋耀辉, 陈兆峰, 等. miR-20a-5p/miR-20b-5p在胃癌细胞和组织中的表达及调控靶基因信号通路富集分析. 临床检验杂志, 2017, 35(11): 822-827.
8. 郗洪庆, 张珂诚, 卫勃, 等. 胃癌TNM分期第八版更新在临床诊断治疗中的意义和思考. 中华胃肠外科杂志, 2017, 20(2): 166-170.
9. 康博雄, 李海龙, 陈彻, 等. miR-106a-5p在胃癌细胞和胃癌组织中的表达及其调控靶基因信号通路富集分析. 中国普外基础与临床杂志, 2018, 25(8): 923-928.
10. Li H, Xie S, Liu M, et al. The clinical significance of down-regulation of mir-124-3p, mir-146a-5p, mir-155-5p and mir-335-5p in gastric cancer tumorigenesis. Int J Oncol, 2014, 45(1): 197-208.
11. Li X, Luo F, Li Q, et al. Identification of new aberrantly expressed miRNAs in intestinal-type gastric cancer and its clinical significance. Oncol Rep, 2011, 26(6): 1431-1439.
12. Zhou LL, Dong JL, Huang G, et al. MicroRNA-143 inhibits cell growth by targeting ERK5 and MAP3K7 in breast cancer. Braz J Med Biol Res, 2017, 50(8): e5891.
13. Jin YP, Hu YP, Wu XS, et al. miR-143-3p targeting of ITGA6 suppresses tumour growth and angiogenesis by downregulating PLGF expression via the PI3K/AKT pathway in gallbladder carcinoma. Cell Death Dis, 2018, 9(2): 182.
14. Dong X, Lv B, Li Y, et al. MiR-143 regulates the proliferation and migration of osteosarcoma cells through targeting MAPK7. Arch Biochem Biophys, 2017, 630: 47-53.
15. Sun X, Dai G, Yu L, et al. miR-143-3p inhibits the proliferation, migration and invasion in osteosarcoma by targeting FOSL2. Sci Rep, 2018, 8(1): 606.
16. Shi H, Shen H, Xu J, et al. MiR-143-3p suppresses the progression of ovarian cancer. Am J Transl Res, 2018, 10(3): 866-874.
17. Panza E, Ercolano G, De Cicco P, et al. MicroRNA-143-3p inhibits growth and invasiveness of melanoma cells by targeting cyclooxygenase-2 and inversely correlates with malignant melanoma progression. Biochem Pharmacol, 2018, 156: 52-59.
18. Huang SJ, Zhu YF, Liu Z, et al. Study of miR-143 expression in stomach cancer. Oncol Lett, 2018, 16(4): 4367-4371.
19. 梁莹, 叶延程, 张文华, 等. 2008~2012年甘肃省武威市凉州区胃癌发病与死亡分析. 中国肿瘤, 2016, 25(7): 519-523.
20. Lei C, Du F, Sun L, et al. miR-143 and miR-145 inhibit gastric cancer cell migration and metastasis by suppressing MYO6. Cell Death Dis, 2017, 8(10): e3101.
21. Zhang Q, Feng Y, Liu P, et al. MiR-143 inhibits cell proliferation and invasion by targeting DNMT3A in gastric cancer. Tumour Biol, 2017, 39(7): 1010428317711312.
22. Wu XL, Cheng B, Li PY, et al. MicroRNA-143 suppresses gastric cancer cell growth and induces apoptosis by targeting COX-2. World J Gastroenterol, 2013, 19(43): 7758-7765.
23. Guoping M, Ran L, Yanru Q. miR-143 inhibits cell proliferation of gastric cancer cells through targeting GATA6. Oncol Res, 2018, 26(7): 1023-1029.
24. Zhuang M, Shi Q, Zhang X, et al. Involvement of miR-143 in cisplatin resistance of gastric cancer cells via targeting IGF1R and BCL2. Tumour Biol, 2015, 36(4): 2737-2745.
25. Xu B, Niu X, Zhang X, et al. miR-143 decreases prostate cancer cells proliferation and migration and enhances their sensitivity to docetaxel through suppression of KRAS. Mol Cell Biochem, 2011, 350(1-2): 207-213.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

The expression and clinical significance of miR-143-3p in gastric cancer tissues with bioinformatical analysis

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content