Effects of short hairpin RNA-mediated CNTN1 gene silencing on growth of transplanted tumors in nude mice_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

HE Sai ¹ , GENG Jie ² , YANG Xiaomin ¹ , WANG Huxia ¹ , HAN Pihua ¹ , HOU Yanni ¹ , FAN Yongguo ¹ ,  CHEN Nan ¹

1. Department of Breast Oncology, Shaanxi Provincial Cancer Hospital, Xi’an 710061, P. R. China;
2. Department of Cardiovascular Internal Medicine, The Second Affiliated Hospital of Medical College of Xi’an Jiaotong University, Xi’an 710004, P. R. China;

Corresponding author：

CHEN Nan, Email: 20795846@qq.com

Keywords：

breast cancer; MDA-MB-468 cell; nude mice; xenograft tumor; contactin-1; Ki-67 protein

DOI：

10.7507/1007-9424.202002056

Video：

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Objective To investigate the inhibitory effect of short hairpin RNA (shRNA) mediated contactin-1 (CNTN1) gene silencing on growth of human breast cancer cell line MDA-MB-468 transplanted tumors in nude mice.Methods Eighteen nude mice (4-week-old male BALB/c) were randomly equally divided into three groups: blank control group, empty vector group, and silencing group. The MDA-MB-468 cells (blank control group), MDA-MB-468 cells transfected by nonsense shRNA (empty vector group), and MDA-MB-468 cells transfected by shRNA (silencing group) were collected in the logarithmic growth period, respectively. The subcutaneous tumor models of nude mice were prepared by the subcutaneous injection of the different group cells. The tumor growth was observed and the expressions of CNTN1 and Ki-67 proteins in the transplanted tumor were detected by the immunohistochemistry.Results The xenograft models of human breast cancer cells were established successfully. The tumor growth in the silencing group was significantly slower than that of the other two groups at every 3 d point (P<0.05). The tumor volume and the tumor weight in the silencing group were significantly smaller or slighter than those of the other two groups at day 18 (P<0.05). The positive rates of CNTN1 and Ki-67 protein expressions in the tumor tissues of the silencing group were lower than those of the other two groups (P<0.05), respectively.Conclusion Silencing expression of CNTN1 gene might inhibit growth of breast cancer cell line MDA-MB-468 transplanted tumors in mude mice.

Citation： HE Sai, GENG Jie, YANG Xiaomin, WANG Huxia, HAN Pihua, HOU Yanni, FAN Yongguo, CHEN Nan. Effects of short hairpin RNA-mediated CNTN1 gene silencing on growth of transplanted tumors in nude mice. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2020, 27(11): 1371-1374. doi: 10.7507/1007-9424.202002056 Copy

1.	Hooper RC, Hsu J, Duncan A, et al. Breast cancer knowledge and decisions made for contralateral prophylactic mastectomy: a survey of surgeons and women in the General population. Plast Reconstr Surg, 2019, 143(5): 936e-945e.
2.	Lee J. A meta-analysis of the association between physical activity and breast cancer mortality. Cancer Nurs, 2019, 42(4): 271-285.
3.	李宗熹, 种姝伊, 古丽米然·阿里同别克, 等. 舒林酸衍生物K-80003与MEK抑制剂考比替尼联合用药对乳腺癌的效果研究. 中国药理学通报, 2019, 35(2): 251-254.
4.	Haenisch C, Diekmann H, Klinger M, et al. The neuronal growth and regeneration associated Cntn1(F3/F11/Contactin) gene is duplicated in fish: expression during development and retinal axon regeneration. Mol Cell Neurosci, 2005, 28(2): 361-374.
5.	Yan J, Ojo D, Kapoor A, et al. Neural cell adhesion protein CNTN1 promotes the metastatic progression of prostate cancer. Cancer Research, 2016, 76(6): 1603-14.
6.	Chen DH, Wei J, Jiang BJ. Contactin 1: A potential therapeutic target and biomarker in gastric cancer. World J Gastroenterol, 2015, 33: 9707-9716.
7.	Xu S, Lam S, Cheng P, et al. Contactin 1 modulates pegylated arginase resistance in small cell lung cancer through induction of epithelial-mesenchymal transition. Scientific Reports, 2019, 9(1): 2031-2044.
8.	Chen N, He S, Ding YN, et al. Effects of recombinant plasmid of pEGFP-N1-CNTN1 on proliferation, clone formation, migration and invasion of Hs578T cells. Modern Oncol, 2016, 24(19): 3017-3020.
9.	Span PN, Sweep FC, Wiegerinck ET, et al. Survivin is an independent prognostic marker for risk stratification of breast cancer patients. Clin Chem, 2004, 50(11): 1986-1993.
10.	Stone JK, Kim JH, Vukadin L, et al. Hypoxia induces cancer cell-specific chromatin interactions and increases MALAT1 expression in breast cancer cells. J Biol Chem, 2019, 294(29): 11213-11224.
11.	Révillion F, Hornez L, Peyrat JP. Quantification of c-erbB-2 gene expression in breast cancer by competitive RT-PCR. Clin Chem, 1997, 43(11): 2114-2120.
12.	Ramirez JM, Fehm T, Orsini M, et al. Prognostic relevance of viable circulating tumor cells detected by EPISPOT in metastatic breast cancer patients. Clin Chem, 2014, 60(1): 214-221.
13.	任云晓, 肖茹丹, 娄晓敏, 等. 基因编辑技术及其在基因治疗中的应用. 遗传, 2019, 41(1): 18-27.
14.	Lei JT, Shao J, Zhang J, et al. Abstract PD8-03: ESR1 gene fusions drive endocrine therapy resistance and metastasis in breast cancer. Cancer Res, 2018, 78(4 Supplement): PD8-03-PD8-03.
15.	Srinivas PR, Srivastava S, Hanash S, et al. Proteomics in early detection of cancer. Clin Chem, 2001, 47(10): 1901-1911.
16.	Illa I. ARTHUR ASBURY LECTURE: Chronic inflammatory demyelinating polyradiculoneuropathy: clinical aspects and new animal models of auto-immunity to nodal components. J Peripher Nerv Syst, 2017, 22(4): 418-424.
17.	Poot M. Intragenic CNTNAP2 deletions: A bridge too far. Mol Syndromol, 2017, 8(3): 118-130.
18.	Anderson C, Gerding WM, Fraenz C, et al. PLP1 and CNTN1 gene variation modulates the microstructure of human white matter in the corpus callosum. Brain Struct Funct, 2018, 223(8): 3875-3887.
19.	Berglund EO, Ranscht B. Molecular cloning and in situ localization of the human contactin gene (CNTN1) on chromosome 12q11-q12. Genomics, 1994, 21(3): 571-582.
20.	陈德虎, 俞继卫, 姜波健. 接触蛋白-1在实体肿瘤中的作用. 世界华人消化杂志, 2015, 23(30): 4785-4791.
21.	Su JL, Yang PC, Shih JY, et al. The VEGF-C/Flt-4 axis promotes invasion and metastasis of cancer cells. Cancer Cell, 2006, 9(3): 209-223.
22.	Wu HM, Cao W, Ye DX, et al. Contactin 1(CNTN1) expression associates with regional lymph node metastasis and is a novel predictor of prognosis in patients with oral squamous cell carcinoma. Mol Med Rep, 2012, 6(2): 265-270.
23.	Liu PF, Chen S, Wu WT, et al. Contactin-1(CNTN-1) overexpression is correlated with advanced clinical stage and lymph node metastasis in oesophageal squamous cell carcinomas. Jpn J Clin Oncol, 2012, 42(7): 612-618.
24.	Yu JW, Wu SH, Lu RQ, et al. Expression and significances of contactin-1 in human gastric cancer. Gastroenterol Res Pract, 2013, 2013: 210205.
25.	Benson JR, Jatoi I. The global breast cancer burden. Future Oncol, 2012, 8(6): 697-702.

1. Hooper RC, Hsu J, Duncan A, et al. Breast cancer knowledge and decisions made for contralateral prophylactic mastectomy: a survey of surgeons and women in the General population. Plast Reconstr Surg, 2019, 143(5): 936e-945e.
2. Lee J. A meta-analysis of the association between physical activity and breast cancer mortality. Cancer Nurs, 2019, 42(4): 271-285.
3. 李宗熹, 种姝伊, 古丽米然·阿里同别克, 等. 舒林酸衍生物K-80003与MEK抑制剂考比替尼联合用药对乳腺癌的效果研究. 中国药理学通报, 2019, 35(2): 251-254.
4. Haenisch C, Diekmann H, Klinger M, et al. The neuronal growth and regeneration associated Cntn1(F3/F11/Contactin) gene is duplicated in fish: expression during development and retinal axon regeneration. Mol Cell Neurosci, 2005, 28(2): 361-374.
5. Yan J, Ojo D, Kapoor A, et al. Neural cell adhesion protein CNTN1 promotes the metastatic progression of prostate cancer. Cancer Research, 2016, 76(6): 1603-14.
6. Chen DH, Wei J, Jiang BJ. Contactin 1: A potential therapeutic target and biomarker in gastric cancer. World J Gastroenterol, 2015, 33: 9707-9716.
7. Xu S, Lam S, Cheng P, et al. Contactin 1 modulates pegylated arginase resistance in small cell lung cancer through induction of epithelial-mesenchymal transition. Scientific Reports, 2019, 9(1): 2031-2044.
8. Chen N, He S, Ding YN, et al. Effects of recombinant plasmid of pEGFP-N1-CNTN1 on proliferation, clone formation, migration and invasion of Hs578T cells. Modern Oncol, 2016, 24(19): 3017-3020.
9. Span PN, Sweep FC, Wiegerinck ET, et al. Survivin is an independent prognostic marker for risk stratification of breast cancer patients. Clin Chem, 2004, 50(11): 1986-1993.
10. Stone JK, Kim JH, Vukadin L, et al. Hypoxia induces cancer cell-specific chromatin interactions and increases MALAT1 expression in breast cancer cells. J Biol Chem, 2019, 294(29): 11213-11224.
11. Révillion F, Hornez L, Peyrat JP. Quantification of c-erbB-2 gene expression in breast cancer by competitive RT-PCR. Clin Chem, 1997, 43(11): 2114-2120.
12. Ramirez JM, Fehm T, Orsini M, et al. Prognostic relevance of viable circulating tumor cells detected by EPISPOT in metastatic breast cancer patients. Clin Chem, 2014, 60(1): 214-221.
13. 任云晓, 肖茹丹, 娄晓敏, 等. 基因编辑技术及其在基因治疗中的应用. 遗传, 2019, 41(1): 18-27.
14. Lei JT, Shao J, Zhang J, et al. Abstract PD8-03: ESR1 gene fusions drive endocrine therapy resistance and metastasis in breast cancer. Cancer Res, 2018, 78(4 Supplement): PD8-03-PD8-03.
15. Srinivas PR, Srivastava S, Hanash S, et al. Proteomics in early detection of cancer. Clin Chem, 2001, 47(10): 1901-1911.
16. Illa I. ARTHUR ASBURY LECTURE: Chronic inflammatory demyelinating polyradiculoneuropathy: clinical aspects and new animal models of auto-immunity to nodal components. J Peripher Nerv Syst, 2017, 22(4): 418-424.
17. Poot M. Intragenic CNTNAP2 deletions: A bridge too far. Mol Syndromol, 2017, 8(3): 118-130.
18. Anderson C, Gerding WM, Fraenz C, et al. PLP1 and CNTN1 gene variation modulates the microstructure of human white matter in the corpus callosum. Brain Struct Funct, 2018, 223(8): 3875-3887.
19. Berglund EO, Ranscht B. Molecular cloning and in situ localization of the human contactin gene (CNTN1) on chromosome 12q11-q12. Genomics, 1994, 21(3): 571-582.
20. 陈德虎, 俞继卫, 姜波健. 接触蛋白-1在实体肿瘤中的作用. 世界华人消化杂志, 2015, 23(30): 4785-4791.
21. Su JL, Yang PC, Shih JY, et al. The VEGF-C/Flt-4 axis promotes invasion and metastasis of cancer cells. Cancer Cell, 2006, 9(3): 209-223.
22. Wu HM, Cao W, Ye DX, et al. Contactin 1(CNTN1) expression associates with regional lymph node metastasis and is a novel predictor of prognosis in patients with oral squamous cell carcinoma. Mol Med Rep, 2012, 6(2): 265-270.
23. Liu PF, Chen S, Wu WT, et al. Contactin-1(CNTN-1) overexpression is correlated with advanced clinical stage and lymph node metastasis in oesophageal squamous cell carcinomas. Jpn J Clin Oncol, 2012, 42(7): 612-618.
24. Yu JW, Wu SH, Lu RQ, et al. Expression and significances of contactin-1 in human gastric cancer. Gastroenterol Res Pract, 2013, 2013: 210205.
25. Benson JR, Jatoi I. The global breast cancer burden. Future Oncol, 2012, 8(6): 697-702.

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Effects of short hairpin RNA-mediated CNTN1 gene silencing on growth of transplanted tumors in nude mice

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