Value of serum LncRNA ANRIL level in the diagnosis of ulcerative colitis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 WANG Yanqiu ¹ , XU Lili ² , JIANG Tao ¹ , XUE Juan ¹

1. Department of Laboratory, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471000, P. R. China;
2. Institute of Genetics and Reproductive Medicine, Nantong Maternal and Child Health Hospital, Nantong, Jiangsu 226000, P. R. China;

Corresponding author：

WANG Yanqiu, Email: wjm7410z@163.com

Keywords：

ulcerative colitis; long non-coding RNA ANRIL; procalcitonin; diagnostic value

DOI：

10.7507/1007-9424.202103100

Video：

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Objective To investigate the level of serum long non-coding RNA antisense non-coding RNA INK4 locus (LncRNA ANRIL) in patients with ulcerative colitis (UC), and to analyze the diagnostic value of serum LncRNA ANRIL level in UC. Methods A total of 143 UC patients admitted to the First Affiliated Hospital of Henan University of Science and Technology from February 2015 to November 2019 were retrospectively analyzed, and 145 healthy people with normal physical examination in the First Affiliated Hospital of Henan University of Science and Technology were selected as the control group. The relationship between serum LncRNA ANRIL level and PCT/IL-17 level was analyzed, the serum levels of LncRNA ANRIL, PCT, and IL-17 were compared between the two groups, and their diagnostic value for UC was explored.Results The disease degree of 143 UC patients: 41 cases were mild, 59 cases were moderate, and 43 cases were severe; endoscopic grade: 38 cases were grade Ⅰ, 65 cases were grade Ⅱ, and 40 cases were grade Ⅲ. Compared with the control group, the serum levels of LncRNA ANRIL, PCT, and IL-17 were increased in the UC group (P<0.05); the levels of serum LncRNA ANRIL, PCT, and IL-17 in the UC group increased gradually with the increase of disease severity and endoscopic grade (P<0.05). The serum levels of LncRNA ANRIL were positively correlated with the levels of PCT and IL-17 in the UC patients (r=0.596, P<0.001; r=0.492, P<0.001). The area under the curve (AUC) of serum LncRNA ANRIL level in the diagnosis of UC was 0.851, the cut-off value was 1.29, the sensitivity and specificity were 75.5% and 83.4%, respectively. The AUC of serum LncRNA ANRIL combined with PCT in the diagnosis of UC was 0.898, the corresponding sensitivity and specificity were 81.8% and 87.6%, respectively. The sensitivity and diagnostic value of combination of LncRNA ANRIL and PCT were higher than that of serum LncRNA ANRIL alone (Z=2.102, P=0.036). Conclusions The serum level of LncRNA ANRIL in UC patients is increased, which has a certain diagnostic value, and it combines with PCT can better predict UC.

Citation： WANG Yanqiu, XU Lili, JIANG Tao, XUE Juan. Value of serum LncRNA ANRIL level in the diagnosis of ulcerative colitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2021, 28(11): 1439-1444. doi: 10.7507/1007-9424.202103100 Copy

1.	Fischer S, Rath T, Neurath MF. Inflammatory bowel diseases: Crohn’s disease and ulcerative colitis. Internist (Berl), 2018, 59(7): 681-693.
2.	蒋丽琳, 肖南平, 杨兴平, 等. CTGF mRNA在溃疡性结肠炎组织中的表达及与病情和预后的关系. 国际消化病杂志, 2020, 40(2): 125-129.
3.	Mohammed Vashist N, Samaan M, Mosli MH, et al. Endoscopic scoring indices for evaluation of disease activity in ulcerative colitis. Cochrane Database Syst Rev, 2018, 1(1): 11450-11456.
4.	刘丽, 柳云恩. 溃疡性结肠炎患者血清miR-15表达水平与炎性反应状态及预后的关系. 国际消化病杂志, 2020, 40(1): 41-46.
5.	Yarani R, Mirza AH, Kaur S, et al. The emerging role of lncRNAs in inflammatory bowel disease. Exp Mol Med, 2018, 50(12): 1-14.
6.	Qiao C, Yang L, Wan J, et al. Long noncoding RNA ANRIL contributes to the development of ulcerative colitis by miR-323b-5p/TLR4/MyD88/NF-κB pathway. Biochem Biophys Res Commun, 2019, 508(1): 217-224.
7.	中华中医药学会. 溃疡性结肠炎诊疗指南. 中国中医药现代远程教育, 2011, 9(10): 126-128.
8.	Jain S, Kedia S, Bopanna S, et al. Are Truelove and Witts criteria for diagnosing acute severe colitis relevant for the Indian population? A prospective study. Intest Res, 2018, 16(1): 69-74.
9.	Landy J, Wahed M, Peake ST, et al. Oral tacrolimus as maintenance therapy for refractory ulcerative colitis–an analysis of outcomes in two London tertiary centres. J Crohns Colitis, 2013, 7(11): e516-e521.
10.	Berends SE, Strik AS, Löwenberg M, et al. Clinical pharmacokinetic and pharmacodynamic considerations in the treatment of ulcerative colitis. Clin Pharmacokinet, 2019, 58(1): 15-37.
11.	李晶莹, 孙斐, 刘智娟. 受体相互作用蛋白激酶1在溃疡性结肠炎患者组织中的表达及意义. 中国临床药理学杂志, 2020, 36(23): 3888-3891.
12.	Gonzalez DO, Nwomeh BC. Complications in children with ulcerative colitis undergoing ileal pouch-anal anastomosis. Semin Pediatr Surg, 2017, 26(6): 384-390.
13.	高成超, 程永波. 血小板异常与溃疡性结肠炎关系的研究进展. 山东医药, 2020, 60(35): 109-111.
14.	Lu C, Yang M, Luo F, et al. Prediction of lncRNA-disease associations based on inductive matrix completion. Bioinformatics, 2018, 34(19): 3357-3364.
15.	贺林, 程龙, 周程继, 等. 长链非编码RNA结肠癌相关转录因子1与胃癌的研究进展. 中国普外基础与临床杂志, 2020, 27(9): 1180-1183.
16.	Zacharopoulou E, Gazouli M, Tzouvala M, et al. The contribution of long non-coding RNAs in inflammatory bowel diseases. Dig Liver Dis, 2017, 49(10): 1067-1072.
17.	孟云超, 牛桂军. 炎症性肠病中长链非编码RNA的研究进展. 重庆医学, 2019, 48(10): 1741-1744.
18.	彭志浩, 李玉明. 长链非编码RNA与结肠癌发生、发展关系的研究进展. 医学研究杂志, 2020, 49(3): 9-11.
19.	Chen SW, Wang PY, Liu YC, et al. Effect of long noncoding RNA H19 overexpression on intestinal barrier function and its potential role in the pathogenesis of ulcerative colitis. Inflamm Bowel Dis, 2016, 22(11): 2582-2592.
20.	Zhou T, Wu L, Zong Z, et al. Long non-coding RNA mortal obligate RNA transcript inhibits the migration and invasion of colon cancer cells by inactivating transforming growth factor β1. Oncol Lett, 2020, 19(2): 1131-1136.
21.	Sun Y, Zheng ZP, Li H, et al. ANRIL is associated with the survival rate of patients with colorectal cancer, and affects cell migration and invasion in vitro. Mol Med Rep, 2016, 14(2): 1714-1720.
22.	Liu XW, Ma T, Liu W, et al. Sustained increase in angiopoietin-2, heparin-binding protein, and procalcitonin is associated with severe sepsis. J Crit Care, 2018, 45(1): 14-19.
23.	张涛, 朱孝明, 杨俊玲. D-二聚体、PCT和CRP检测在重症胰腺炎中的临床价值. 解放军预防医学杂志, 2020, 38(1): 17-18.
24.	Wu HM, Wei J, Li J, et al. Serum procalcitonin as a potential early predictor of short-term outcomes in acute severe ulcerative colitis. Dig Dis Sci, 2019, 64(11): 3263-3273.
25.	Lee MH, Tung-Chieh Chang J, Liao CT, et al. Interleukin 17 and peripheral IL-17-expressing T cells are negatively correlated with the overall survival of head and neck cancer patients. Oncotarget, 2018, 9(11): 9825-9837.
26.	马乾章, 李云, 丁原全. 白头翁汤加味联合美沙拉嗪缓释片治疗热毒炽盛型溃疡性结肠炎的临床疗效观察. 海南医学院学报, 2019, 25(12): 918-922.

1. Fischer S, Rath T, Neurath MF. Inflammatory bowel diseases: Crohn’s disease and ulcerative colitis. Internist (Berl), 2018, 59(7): 681-693.
2. 蒋丽琳, 肖南平, 杨兴平, 等. CTGF mRNA在溃疡性结肠炎组织中的表达及与病情和预后的关系. 国际消化病杂志, 2020, 40(2): 125-129.
3. Mohammed Vashist N, Samaan M, Mosli MH, et al. Endoscopic scoring indices for evaluation of disease activity in ulcerative colitis. Cochrane Database Syst Rev, 2018, 1(1): 11450-11456.
4. 刘丽, 柳云恩. 溃疡性结肠炎患者血清miR-15表达水平与炎性反应状态及预后的关系. 国际消化病杂志, 2020, 40(1): 41-46.
5. Yarani R, Mirza AH, Kaur S, et al. The emerging role of lncRNAs in inflammatory bowel disease. Exp Mol Med, 2018, 50(12): 1-14.
6. Qiao C, Yang L, Wan J, et al. Long noncoding RNA ANRIL contributes to the development of ulcerative colitis by miR-323b-5p/TLR4/MyD88/NF-κB pathway. Biochem Biophys Res Commun, 2019, 508(1): 217-224.
7. 中华中医药学会. 溃疡性结肠炎诊疗指南. 中国中医药现代远程教育, 2011, 9(10): 126-128.
8. Jain S, Kedia S, Bopanna S, et al. Are Truelove and Witts criteria for diagnosing acute severe colitis relevant for the Indian population? A prospective study. Intest Res, 2018, 16(1): 69-74.
9. Landy J, Wahed M, Peake ST, et al. Oral tacrolimus as maintenance therapy for refractory ulcerative colitis–an analysis of outcomes in two London tertiary centres. J Crohns Colitis, 2013, 7(11): e516-e521.
10. Berends SE, Strik AS, Löwenberg M, et al. Clinical pharmacokinetic and pharmacodynamic considerations in the treatment of ulcerative colitis. Clin Pharmacokinet, 2019, 58(1): 15-37.
11. 李晶莹, 孙斐, 刘智娟. 受体相互作用蛋白激酶1在溃疡性结肠炎患者组织中的表达及意义. 中国临床药理学杂志, 2020, 36(23): 3888-3891.
12. Gonzalez DO, Nwomeh BC. Complications in children with ulcerative colitis undergoing ileal pouch-anal anastomosis. Semin Pediatr Surg, 2017, 26(6): 384-390.
13. 高成超, 程永波. 血小板异常与溃疡性结肠炎关系的研究进展. 山东医药, 2020, 60(35): 109-111.
14. Lu C, Yang M, Luo F, et al. Prediction of lncRNA-disease associations based on inductive matrix completion. Bioinformatics, 2018, 34(19): 3357-3364.
15. 贺林, 程龙, 周程继, 等. 长链非编码RNA结肠癌相关转录因子1与胃癌的研究进展. 中国普外基础与临床杂志, 2020, 27(9): 1180-1183.
16. Zacharopoulou E, Gazouli M, Tzouvala M, et al. The contribution of long non-coding RNAs in inflammatory bowel diseases. Dig Liver Dis, 2017, 49(10): 1067-1072.
17. 孟云超, 牛桂军. 炎症性肠病中长链非编码RNA的研究进展. 重庆医学, 2019, 48(10): 1741-1744.
18. 彭志浩, 李玉明. 长链非编码RNA与结肠癌发生、发展关系的研究进展. 医学研究杂志, 2020, 49(3): 9-11.
19. Chen SW, Wang PY, Liu YC, et al. Effect of long noncoding RNA H19 overexpression on intestinal barrier function and its potential role in the pathogenesis of ulcerative colitis. Inflamm Bowel Dis, 2016, 22(11): 2582-2592.
20. Zhou T, Wu L, Zong Z, et al. Long non-coding RNA mortal obligate RNA transcript inhibits the migration and invasion of colon cancer cells by inactivating transforming growth factor β1. Oncol Lett, 2020, 19(2): 1131-1136.
21. Sun Y, Zheng ZP, Li H, et al. ANRIL is associated with the survival rate of patients with colorectal cancer, and affects cell migration and invasion in vitro. Mol Med Rep, 2016, 14(2): 1714-1720.
22. Liu XW, Ma T, Liu W, et al. Sustained increase in angiopoietin-2, heparin-binding protein, and procalcitonin is associated with severe sepsis. J Crit Care, 2018, 45(1): 14-19.
23. 张涛, 朱孝明, 杨俊玲. D-二聚体、PCT和CRP检测在重症胰腺炎中的临床价值. 解放军预防医学杂志, 2020, 38(1): 17-18.
24. Wu HM, Wei J, Li J, et al. Serum procalcitonin as a potential early predictor of short-term outcomes in acute severe ulcerative colitis. Dig Dis Sci, 2019, 64(11): 3263-3273.
25. Lee MH, Tung-Chieh Chang J, Liao CT, et al. Interleukin 17 and peripheral IL-17-expressing T cells are negatively correlated with the overall survival of head and neck cancer patients. Oncotarget, 2018, 9(11): 9825-9837.
26. 马乾章, 李云, 丁原全. 白头翁汤加味联合美沙拉嗪缓释片治疗热毒炽盛型溃疡性结肠炎的临床疗效观察. 海南医学院学报, 2019, 25(12): 918-922.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Value of serum LncRNA ANRIL level in the diagnosis of ulcerative colitis

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