Development and validation of a preoperative predictive model for microvascular invasion in hepatocellular carcinoma based on lipid metabolism markers_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

XU Lingcong ¹ , ZHENG Yan ¹ , GONG Tianle ² , ZHOU Yongqiang ¹ , YAN Jiexi ³ , DING Fanghui ² ,  LI Xun ^1,2,3,4

1. The First Clinical Medical College of Lanzhou University, Lanzhou 730000, P. R. China;
2. Department of General Surgery, The First Hospital of Lanzhou University, Lanzhou 730000, P. R. China;
3. Precision Medicine Center, The First Hospital of Lanzhou University, Lanzhou 730000, P. R. China;
4. Gansu Province Key Laboratory of Biotherapy and Regenerative Medicine, Lanzhou 730000, P. R. China;

Corresponding author：

LI Xun, Email: lxdr21@126.com

Keywords：

lipid metabolism; microvascular invasion; predictive model; hepatocellular carcinoma

DOI：

10.7507/1007-9424.202310046

Video：

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Objective To investigate the association of lipid metabolism and other markers with microvascular invasion in hepatocellular carcinoma (HCC) and to develop a preoperative prediction model from it. Methods Data from 389 HCC patients who underwent hepatectomy at First Hospital of Lanzhou University between January 2017 and March 2023 were retrospectively analyzed. These patients were divided into training group (n=272) and validation group (n=117) with a ratio of 7 : 3. The independent risk factors of microvascular invasion (MVI) were determined by univariate and multivariate logistic regression analysis, and the MVI prediction model was established. The prediction efficiency of the model was verified by the analysis of calibration curve, receiver operating characteristic (ROC) curve and decision curve. Results Univariate and multivariate logistic regression analysis showed that the risk factors independently related to MVI before operation included total cholesterol, lactate dehydrogenase, body mass index, alpha-fetoprotein, carbohydrate antigen 125, hepatitis B DNA, maximum tumor diameter and albumin-bilirubin score. MVI prediction model was established based on the above eight risk factors, and its area under ROC curve in the training group and the validation group were 0.79 [95%CI (0.74, 0.84)] and 0.75 [95%CI (0.66, 0.84)] respectively. Calibration curve analysis showed that the prediction curve fitted well with the standard curve. ROC curve analysis showed that the MVI prediction model was efficient. Decision curve analysis confirmed that the MVI prediction model had significant clinical applications. Conclusion This study identified independent correlations between total cholesterol levels, among other things, and MVI, and successfully developed and validated novel predictive model based on these indicators that can help physicians effectively identify individuals at high risk for MVI in patients with hepatocellular carcinoma preoperatively, leading to more rational treatment choices.

Citation： XU Lingcong, ZHENG Yan, GONG Tianle, ZHOU Yongqiang, YAN Jiexi, DING Fanghui, LI Xun. Development and validation of a preoperative predictive model for microvascular invasion in hepatocellular carcinoma based on lipid metabolism markers. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2024, 31(3): 320-329. doi: 10.7507/1007-9424.202310046 Copy

1.	Cao W, Chen HD, Yu YW, et al. Changing profiles of cancer burden worldwide and in China: a secondary analysis of the global cancer statistics 2020. Chin Med J (Engl), 2021, 134(7): 783-791.
2.	Sung H, Ferlay J, Siegel RL, et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin, 2021, 71(3): 209-249.
3.	Vogel A, Meyer T, Sapisochin G, et al. Hepatocellular carcinoma. Lancet, 2022, 400(10360): 1345-1362.
4.	Chen ZH, Zhang XP, Wang H, et al. Effect of microvascular invasion on the postoperative long-term prognosis of solitary small HCC: a systematic review and meta-analysis. HPB (Oxford), 2019, 21(8): 935-944.
5.	Wu F, Sun H, Zhou C, et al. Prognostic factors for long-term outcome in bifocal hepatocellular carcinoma after resection. Eur Radiol, 2023, 33(5): 3604-3616.
6.	Han J, Li ZL, Xing H, et al. The impact of resection margin and microvascular invasion on long-term prognosis after curative resection of hepatocellular carcinoma: a multi-institutional study. HPB (Oxford), 2019, 21(8): 962-971.
7.	Yang P, Si A, Yang J, et al. A wide-margin liver resection improves long-term outcomes for patients with HBV-related hepatocellular carcinoma with microvascular invasion. Surgery, 2019, 165(4): 721-730.
8.	Zhou JM, Zhou CY, Chen XP, et al. Anatomic resection improved the long-term outcome of hepatocellular carcinoma patients with microvascular invasion: A prospective cohort study. World J Gastrointest Oncol, 2021, 13(12): 2190-2202.
9.	European Association for the Study of the Liver. EASL Clinical Practice Guidelines: Management of hepatocellular carcinoma. J Hepatol, 2018, 69(1): 182-236.
10.	Lee S, Kang TW, Song KD, et al. Effect of microvascular invasion risk on early recurrence of hepatocellular carcinoma after surgery and radiofrequency ablation. Ann Surg, 2021, 273(3): 564-571.
11.	Mazzaferro V, Llovet JM, Miceli R, et al. Predicting survival after liver transplantation in patients with hepatocellular carcinoma beyond the Milan criteria: a retrospective, exploratory analysis. Lancet Oncol, 2009, 10(1): 35-43.
12.	Iguchi T, Shirabe K, Aishima S, et al. New pathologic stratification of microvascular invasion in hepatocellular carcinoma: Predicting prognosis after living-donor liver transplantation. Transplantation, 2015, 99(6): 1236-1242.
13.	Bian X, Liu R, Meng Y, et al. Lipid metabolism and cancer. J Exp Med, 2021, 218(1): e20201606.
14.	Broadfield LA, Duarte JAG, Schmieder R, et al. Fat induces glucose metabolism in nontransformed liver cells and promotes liver tumorigenesis. Cancer Res, 2021, 81(8): 1988-2001.
15.	Guo Z, Liang J. Lipid-Based Factors: A promising new biomarker for predicting prognosis and conditional survival probability in hepatocellular carcinoma. J Hepatocell Carcinoma, 2022, 9: 869-883.
16.	Liang JQ, Teoh N, Xu L, et al. Dietary cholesterol promotes steatohepatitis related hepatocellular carcinoma through dysregulated metabolism and calcium signaling. Nat Commun, 2018, 9(1): 4490.
17.	中华人民共和国国家卫生健康委员会. 原发性肝癌诊疗指南(2022年版). 肿瘤综合治疗电子杂志, 2022, 8(2): 16-53.
18.	中国抗癌协会肝癌专业委员会, 中华医学会肝病学分会肝癌学组, 中国抗癌协会病理专业委员会, 等. 原发性肝癌规范化病理诊断指南(2015年版). 临床肝胆病杂志, 2015, 31(6): 833-839.
19.	Tian Y, Hua H, Peng Q, et al. Preoperative evaluation of Gd-EOB-DTPA-enhanced MRI radiomics-based nomogram in small solitary hepatocellular carcinoma (≤3 cm) with microvascular invasion: A two-center study. J Magn Reson Imaging, 2022, 56(5): 1459-1472.
20.	Lei Z, Li J, Wu D, et al. Nomogram for preoperative estimation of microvascular invasion risk in hepatitis B virus-related hepatocellular carcinoma within the milan criteria. JAMA Surg, 2016, 151(4): 356-363.
21.	Yan Y, Zhou Q, Zhang M, et al. Integrated nomograms for preoperative prediction of microvascular invasion and lymph node metastasis risk in hepatocellular carcinoma patients. Ann Surg Oncol, 2020, 27(5): 1361-1371.
22.	Kimura Y, Sumiyoshi M. High-fat, high-sucrose, and high-cholesterol diets accelerate tumor growth and metastasis in tumor-bearing mice. Nutr Cancer, 2007, 59(2): 207-216.
23.	Yang SY, Wang CC, Chen KD, et al. Statin use is associated with a lower risk of recurrence after curative resection in BCLC stage 0-A hepatocellular carcinoma. BMC Cancer, 2021, 21(1): 70.
24.	Mathur A, Franco ES, Leone JP, et al. Obesity portends increased morbidity and earlier recurrence following liver transplantation for hepatocellular carcinoma. HPB (Oxford), 2013, 15(7): 504-510.
25.	Huang Y, Huang X, Zeng J, et al. Knockdown of MUC16 (CA125) enhances the migration and invasion of hepatocellular carcinoma cells. Front Oncol, 2021, 1: 667669.
26.	Baert T, Van Camp J, Vanbrabant L, et al. Influence of CA125, platelet count and neutrophil to lymphocyte ratio on the immune system of ovarian cancer patients. Gynecol Oncol, 2018, 150(1): 31-37.
27.	Pérez-Tomás R, Pérez-Guillén I. Lactate in the tumor microenvironment: An essential molecule in cancer progression and treatment. Cancers (Basel), 2020, 12(11): 3244.
28.	Bağırsakçı E, Şahin E, Atabey N, et al. Role of albumin in growth inhibition in hepatocellular carcinoma. Oncology, 2017, 93(2): 136-142.
29.	Fujiwara R, Haag M, Schaeffeler E, et al. Systemic regulation of bilirubin homeostasis: Potential benefits of hyperbilirubinemia. Hepatology, 2018, 67(4): 1609-1619.
30.	Yao Q, Jiang X, Zhai YY, et al. Protective effects and mechanisms of bilirubin nanomedicine against acute pancreatitis. J Control Release, 2020, 322: 312-325.

1. Cao W, Chen HD, Yu YW, et al. Changing profiles of cancer burden worldwide and in China: a secondary analysis of the global cancer statistics 2020. Chin Med J (Engl), 2021, 134(7): 783-791.
2. Sung H, Ferlay J, Siegel RL, et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin, 2021, 71(3): 209-249.
3. Vogel A, Meyer T, Sapisochin G, et al. Hepatocellular carcinoma. Lancet, 2022, 400(10360): 1345-1362.
4. Chen ZH, Zhang XP, Wang H, et al. Effect of microvascular invasion on the postoperative long-term prognosis of solitary small HCC: a systematic review and meta-analysis. HPB (Oxford), 2019, 21(8): 935-944.
5. Wu F, Sun H, Zhou C, et al. Prognostic factors for long-term outcome in bifocal hepatocellular carcinoma after resection. Eur Radiol, 2023, 33(5): 3604-3616.
6. Han J, Li ZL, Xing H, et al. The impact of resection margin and microvascular invasion on long-term prognosis after curative resection of hepatocellular carcinoma: a multi-institutional study. HPB (Oxford), 2019, 21(8): 962-971.
7. Yang P, Si A, Yang J, et al. A wide-margin liver resection improves long-term outcomes for patients with HBV-related hepatocellular carcinoma with microvascular invasion. Surgery, 2019, 165(4): 721-730.
8. Zhou JM, Zhou CY, Chen XP, et al. Anatomic resection improved the long-term outcome of hepatocellular carcinoma patients with microvascular invasion: A prospective cohort study. World J Gastrointest Oncol, 2021, 13(12): 2190-2202.
9. European Association for the Study of the Liver. EASL Clinical Practice Guidelines: Management of hepatocellular carcinoma. J Hepatol, 2018, 69(1): 182-236.
10. Lee S, Kang TW, Song KD, et al. Effect of microvascular invasion risk on early recurrence of hepatocellular carcinoma after surgery and radiofrequency ablation. Ann Surg, 2021, 273(3): 564-571.
11. Mazzaferro V, Llovet JM, Miceli R, et al. Predicting survival after liver transplantation in patients with hepatocellular carcinoma beyond the Milan criteria: a retrospective, exploratory analysis. Lancet Oncol, 2009, 10(1): 35-43.
12. Iguchi T, Shirabe K, Aishima S, et al. New pathologic stratification of microvascular invasion in hepatocellular carcinoma: Predicting prognosis after living-donor liver transplantation. Transplantation, 2015, 99(6): 1236-1242.
13. Bian X, Liu R, Meng Y, et al. Lipid metabolism and cancer. J Exp Med, 2021, 218(1): e20201606.
14. Broadfield LA, Duarte JAG, Schmieder R, et al. Fat induces glucose metabolism in nontransformed liver cells and promotes liver tumorigenesis. Cancer Res, 2021, 81(8): 1988-2001.
15. Guo Z, Liang J. Lipid-Based Factors: A promising new biomarker for predicting prognosis and conditional survival probability in hepatocellular carcinoma. J Hepatocell Carcinoma, 2022, 9: 869-883.
16. Liang JQ, Teoh N, Xu L, et al. Dietary cholesterol promotes steatohepatitis related hepatocellular carcinoma through dysregulated metabolism and calcium signaling. Nat Commun, 2018, 9(1): 4490.
17. 中华人民共和国国家卫生健康委员会. 原发性肝癌诊疗指南(2022年版). 肿瘤综合治疗电子杂志, 2022, 8(2): 16-53.
18. 中国抗癌协会肝癌专业委员会, 中华医学会肝病学分会肝癌学组, 中国抗癌协会病理专业委员会, 等. 原发性肝癌规范化病理诊断指南(2015年版). 临床肝胆病杂志, 2015, 31(6): 833-839.
19. Tian Y, Hua H, Peng Q, et al. Preoperative evaluation of Gd-EOB-DTPA-enhanced MRI radiomics-based nomogram in small solitary hepatocellular carcinoma (≤3 cm) with microvascular invasion: A two-center study. J Magn Reson Imaging, 2022, 56(5): 1459-1472.
20. Lei Z, Li J, Wu D, et al. Nomogram for preoperative estimation of microvascular invasion risk in hepatitis B virus-related hepatocellular carcinoma within the milan criteria. JAMA Surg, 2016, 151(4): 356-363.
21. Yan Y, Zhou Q, Zhang M, et al. Integrated nomograms for preoperative prediction of microvascular invasion and lymph node metastasis risk in hepatocellular carcinoma patients. Ann Surg Oncol, 2020, 27(5): 1361-1371.
22. Kimura Y, Sumiyoshi M. High-fat, high-sucrose, and high-cholesterol diets accelerate tumor growth and metastasis in tumor-bearing mice. Nutr Cancer, 2007, 59(2): 207-216.
23. Yang SY, Wang CC, Chen KD, et al. Statin use is associated with a lower risk of recurrence after curative resection in BCLC stage 0-A hepatocellular carcinoma. BMC Cancer, 2021, 21(1): 70.
24. Mathur A, Franco ES, Leone JP, et al. Obesity portends increased morbidity and earlier recurrence following liver transplantation for hepatocellular carcinoma. HPB (Oxford), 2013, 15(7): 504-510.
25. Huang Y, Huang X, Zeng J, et al. Knockdown of MUC16 (CA125) enhances the migration and invasion of hepatocellular carcinoma cells. Front Oncol, 2021, 1: 667669.
26. Baert T, Van Camp J, Vanbrabant L, et al. Influence of CA125, platelet count and neutrophil to lymphocyte ratio on the immune system of ovarian cancer patients. Gynecol Oncol, 2018, 150(1): 31-37.
27. Pérez-Tomás R, Pérez-Guillén I. Lactate in the tumor microenvironment: An essential molecule in cancer progression and treatment. Cancers (Basel), 2020, 12(11): 3244.
28. Bağırsakçı E, Şahin E, Atabey N, et al. Role of albumin in growth inhibition in hepatocellular carcinoma. Oncology, 2017, 93(2): 136-142.
29. Fujiwara R, Haag M, Schaeffeler E, et al. Systemic regulation of bilirubin homeostasis: Potential benefits of hyperbilirubinemia. Hepatology, 2018, 67(4): 1609-1619.
30. Yao Q, Jiang X, Zhai YY, et al. Protective effects and mechanisms of bilirubin nanomedicine against acute pancreatitis. J Control Release, 2020, 322: 312-325.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Development and validation of a preoperative predictive model for microvascular invasion in hepatocellular carcinoma based on lipid metabolism markers

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