The Effects of Acute and Chronic Ozone Exposure on Inflammation,Structure and Function in Murine Lung_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

LiFeng , ZhangPengyu , ZhangMin , BaoAihua , ChenYuqing , TangYueqin ,  ZhouXin

Department of Respiratory Medicine, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai, 200080, China;

Corresponding author：

ZhouXin, Email: xzhou53@163.com

Keywords：

Ozone; Lung inflammation; Lung structure; Lung function

DOI：

10.7507/1671-6205.2014071

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the effects of acute and chronic ozone exposure on inflammation,structure and function in murine lung. Methods 32 C57/BL6 mice were randomly divided into a single (acute) ozone exposed group,a single air exposed group,a multiple (chronic) ozone exposed group (every three days over 6 weeks),and a multiple air exposed group with 8 mice in each group.The mice were exposed to 2.5 ppm of ozone or air for 3 hours per time and sacrificed 24 hours after the last time of ozone exposure.Lung volume,low attenuation area (LAA) percentage,lung function,cell counts and malondialdehyde (MDA) in bronchoalveolar lavage fluid (BALF),8-hydroxy-2'-deoxyguanosine (8-OHdG) in serum,inflammation scores and mean linear intercept (Lm) in lung section were assessed. Results Compared with the single air exposed group,single (acute) ozone exposure led to increases in inflammatory cells in BALF,inflammation scores in the lung tissue,MDA in BALF and 8-OHdG in serum,but had no effect on lung volume,LAA percentage,airflow or Lm.Compared with the single (acute) ozone exposed group,the single air exposed group and the multiple air exposed group,multiple (chronic) ozone exposure increased inflammatory cells in BALF,lung volume,LAA percentage,total lung capacity and lung compliance,mediated airflow obstruction,and also increased lung inflammation socres and Lm. Conclusion Acute ozone exposure induced airway/lung inflammation and oxidative stress,while chronic ozone exposure induced airway/lung inflammation,emphysema and airflow obstruction.

Citation： LiFeng, ZhangPengyu, ZhangMin, BaoAihua, ChenYuqing, TangYueqin, ZhouXin. The Effects of Acute and Chronic Ozone Exposure on Inflammation,Structure and Function in Murine Lung. Chinese Journal of Respiratory and Critical Care Medicine, 2014, 13(3): 295-299. doi: 10.7507/1671-6205.2014071 Copy

1.	Ko FW,Tam W,Wong TW,et al.Effects of air pollution on asthma hospitalization rates in different age groups in Hong Kong.Clin Exp Allergy,2007,37:1312-1319.
2.	Medina-Ramon MA,Zanobetti A,Schwartz J.The effect of ozone and PM10 on hospital admissions for pneumonia and chronic obstructive pulmonary disease:a national multicity study.Am J Epidemiol,2006,163:579-588.
3.	Mudway IS,Kelly FJ.An investigation of inhaled ozone dose and the magnitude of airway inflammation in healthy adults.Am J Respir Crit Care Med,2004,169:1089-1095.
4.	Alexeeff SE,Litonjua AA,Wright RO,et al.Ozone exposure,antioxidant genes,and lung function in an elderly cohort:VA normative aging study.Occup Environ Med,2008,65:736-742.
5.	Que LG,Stiles JV,Sundy JS,et al.Pulmonary function,bronchial reactivity,and epithelial permeability are response phenotypes to ozone and develop differentially in healthy humans.J Appl Physiol,2011,111:679-687.
6.	Yang W,Omaye ST.Air pollutants,oxidative stress and human health.Mutat Res,2009,674:45-54.
7.	李锋,张旻,周新.氧化应激与慢性气道疾病.中国呼吸与危重监护杂志,2012,11:97-100.
8.	Chung KF,Adcock IM.Multifaceted mechanisms in COPD:inflammation,immunity,and tissue repair and destruction.Eur Respir J,2008,31:1334-1356.
9.	Li F,Wiegman C,Seiffert J,et al. Effects of N-acetylcysteine in ozone-induced chronic obstructive pulmonary disease model.PLoS ONE,2013,8:e80782.
10.	Johnson KA.Imaging techniques for small animal imaging models of pulmonary disease:micro-CT.Toxicol Pathol,2007,35:59-64.
11.	Froese AR,Ask K,Labiris R,et al.Three-dimensional computed tomography imaging in an animal model of emphysema.Eur Respir J,2007,30:1082-1089.
12.	Knudsen L,Weibel ER,Gundersen HJ,et al.Assessment of air space size characteristics by intercept (chord) measurement:an accurate and efficient stereological approach.J Appl Physiol,2010,108:412-421.
13.	Hazucha MJ,Folinsbee LJ,Bromberg PA.Distribution and reproducibility of spirometric response to ozone by gender and age.J Appl Physiol,2003,95:1917-1925.
14.	Tager IB,Balmes J,Lurmann F,et al.Chronic exposure to ambient ozone and lung function in young adults.Epidemiology,2005,16:751-759.

1. Ko FW,Tam W,Wong TW,et al.Effects of air pollution on asthma hospitalization rates in different age groups in Hong Kong.Clin Exp Allergy,2007,37:1312-1319.
2. Medina-Ramon MA,Zanobetti A,Schwartz J.The effect of ozone and PM10 on hospital admissions for pneumonia and chronic obstructive pulmonary disease:a national multicity study.Am J Epidemiol,2006,163:579-588.
3. Mudway IS,Kelly FJ.An investigation of inhaled ozone dose and the magnitude of airway inflammation in healthy adults.Am J Respir Crit Care Med,2004,169:1089-1095.
4. Alexeeff SE,Litonjua AA,Wright RO,et al.Ozone exposure,antioxidant genes,and lung function in an elderly cohort:VA normative aging study.Occup Environ Med,2008,65:736-742.
5. Que LG,Stiles JV,Sundy JS,et al.Pulmonary function,bronchial reactivity,and epithelial permeability are response phenotypes to ozone and develop differentially in healthy humans.J Appl Physiol,2011,111:679-687.
6. Yang W,Omaye ST.Air pollutants,oxidative stress and human health.Mutat Res,2009,674:45-54.
7. 李锋,张旻,周新.氧化应激与慢性气道疾病.中国呼吸与危重监护杂志,2012,11:97-100.
8. Chung KF,Adcock IM.Multifaceted mechanisms in COPD:inflammation,immunity,and tissue repair and destruction.Eur Respir J,2008,31:1334-1356.
9. Li F,Wiegman C,Seiffert J,et al. Effects of N-acetylcysteine in ozone-induced chronic obstructive pulmonary disease model.PLoS ONE,2013,8:e80782.
10. Johnson KA.Imaging techniques for small animal imaging models of pulmonary disease:micro-CT.Toxicol Pathol,2007,35:59-64.
11. Froese AR,Ask K,Labiris R,et al.Three-dimensional computed tomography imaging in an animal model of emphysema.Eur Respir J,2007,30:1082-1089.
12. Knudsen L,Weibel ER,Gundersen HJ,et al.Assessment of air space size characteristics by intercept (chord) measurement:an accurate and efficient stereological approach.J Appl Physiol,2010,108:412-421.
13. Hazucha MJ,Folinsbee LJ,Bromberg PA.Distribution and reproducibility of spirometric response to ozone by gender and age.J Appl Physiol,2003,95:1917-1925.
14. Tager IB,Balmes J,Lurmann F,et al.Chronic exposure to ambient ozone and lung function in young adults.Epidemiology,2005,16:751-759.

Previous Article
Efficacy of Triple Aerosol Inhalation of Pulmicort Respules,Ipratropine and Ventolin in Treatment of Severe Acute Asthma Exacerbations in Adults
Next Article
肺淋巴管肌瘤病一例

Chinese Journal of Respiratory and Critical Care Medicine

The Effects of Acute and Chronic Ozone Exposure on Inflammation,Structure and Function in Murine Lung

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content