Effects of Smoking on Th17/Treg T Cell Subsets and Cytokines Expression in Patients with Chronic Obstructive Pulmonary Disease in Stable Phase_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

XuDan ¹ , GongLi ² , XuQian ¹ , WangJing ¹ , JiangMin ¹ , GaoZhen ¹ ,  LiFengsen ²

1. The COPD Lab of National Clinical Research Base of Traditional Chinese Medicine, Urumqi, Xinjiang, 830000, China;
2. ;

Corresponding author：

LiFengsen, Email: fengsen602@163.com

Keywords：

Chronic obstructive pulmonary disease; Smoking; Th17/Treg; Cytokines

DOI：

10.7507/1671-6205.2015003

Video：

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Objective To investigate the effects of smoking on Th17/Treg T cell subsets and cytokines expression in patients with chronic obstructive pulmonary disease (COPD) in stable stage. Methods From February 2012 to June 2013, sixty outpatients with stable COPD (20 cases of non-smokers, 40 cases of smokers) and 15 normal volunteers were recruited in the study in the Traditional Chinese Medicine Hospital affiliated to Xinjiang Medical University. Th17/Treg level in peripheral blood was detected by flow cytometry method. Cytometric bead array system was used to detect TGF-β, IL-10, IL-17A, IFN-γand other inflammatory factors in serum. Results The patients' age, duration of disease, lung function, disease severity, and other related data were comparable between the smoking COPD group and the non-smoking COPD group (P > 0.05). Th17/Treg level was increased in the smoking COPD group compared with the normal group (P < 0.05), and showed an increasing trend from the normal group to the non-smoking COPD group and the smoking COPD group. The level of IL-2 in the smoking and non-smoking COPD groups was lower than that in the normal group. Compared with the normal group, the level of TNF-αwas significantly decreased in the smoking and non-smoking COPD groups(P < 0.05). Conclusions Systemic inflammatory response continuously exists in patients with COPD even in the stable phase. Smoking can partly enhance the inflammatory reaction in COPD. The Th17/Treg T cell subsets associated cytokine regulation has gradually tended to a balance in the stable phase, and inflammatory factors related recovery speed is not consistent, suggesting that smoking may play a certain role in the recovery of balance.

Citation： XuDan, GongLi, XuQian, WangJing, JiangMin, GaoZhen, LiFengsen. Effects of Smoking on Th17/Treg T Cell Subsets and Cytokines Expression in Patients with Chronic Obstructive Pulmonary Disease in Stable Phase. Chinese Journal of Respiratory and Critical Care Medicine, 2015, 14(1): 5-9. doi: 10.7507/1671-6205.2015003 Copy

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13.	Gamble E, Grootendorst DC, Hattotuwa K, et al.Airway mucosal inflammation in COPD is similar in smokers and ex-smokers:a pooled analysis.Eur Respir J, 2007, 30:467-471.
14.	杨玉书, 刘士林.吸烟与肺部感染在慢性阻塞性肺部疾病中的发病机理探讨.临床肺科杂志, 2009, 14:796-797.
15.	Adair-Kirk TL, Atkinson JJ, Senior RM.Smoke particulates stress lung cells.Nat Med, 2008, 14:1024-1025.
16.	Mangan PR, Harrington IE, Quinn DB, et al.Transforming growth factor-beta induces development of the TH17 Lineage.Nature, 2006, 441:231-234.
17.	姜敏, 田戈, 王晶, 等.维药神香草对COPD小鼠Th1/Th2和Th17/Treg平衡的影响.世界科学技术-中医药现代化, 2013, 15:591-594.
18.	王胜军, 许化溪, 杨胜利.CD4⁺CD25⁺调节性T细胞.细胞生物学杂志, 2005, 27:61-65.
19.	Li MO, Wan YY, Flavell RA.T cell-produced transforming growth factorβ1 controls T cell tolerance and regulates Th1-and Th17-cell differentiation.Immunity, 2007, 26:579-591.

1. 任玲, 朱兰, 王长捷.健康老年人血脂与年龄、性别的关系.现代预防医学, 2003, 30:532-533.
2. Løkke A, Lange P, Scharling H, et al.Developing COPD:a 25 year follow up study of the general population.Thorax, 2006, 61:935-939.
3. 骆树新, 许西琳, 关键.吸烟与慢性阻塞性肺病肺功能相关性研究.中华实用诊断与治疗杂志, 2011, 25:621-622.
4. Harrington LE, Mangan PR, Weaver CT.Expanding the effector CD4 T-cell repertoire:the Th17 lineage.Curr Opin Immunol, 2006, 18:349-56.
5. 李鸿佳, 盖庆玲, 王立华, 等.Th17淋巴细胞在哮喘小鼠气道炎症中的初步研究.细胞与分子免疫学杂志, 2011, 27:53-55.
6. Annunziato F, Cosmi L, SantarlasciV, et al.Phenotypic and functional features of human Th17 cells.J Exp Med, 2007, 204:1849-1861.
7. 刘秀荣, 李玉青, 张朱佳子, 等.北京市疾病预防控制中心系统控烟现状调查.疾病监测, 2009, 24:57-59.
8. Higashimoto Y, Yamagata Y, Taya S, et al.Systemic inflammation in chronic obstructive pulmonary disease and asthma:similarities and differences.Respirology, 2008, 13:128-133.
9. Vemooy JH, Kucukaycan M, Jacobs JA, et al.Local and systemic inflammation in patients with chronic obstructive pulmonary disease.Am J Respir Crit Care Med, 2002, 166:1218-1224.
10. 程霞.COPD稳定期患者血清IL-6、IL-8、hs-CRP水平与吸烟关系探讨.中国临床研究, 2010, 23:273-274.
11. Fletcher C, Peto R.The natural history of chronic airflow obstruction.Br Med J, 1977, 1:1645-1648.
12. Global strategy for the diagnosis, management and prevention of chronic obstructive pulmonary disease (revised 2011)[EB/OL].http://www.goldcopd.org/guidelines-pocket-guide-to-copd-diagnosis.html.
13. Gamble E, Grootendorst DC, Hattotuwa K, et al.Airway mucosal inflammation in COPD is similar in smokers and ex-smokers:a pooled analysis.Eur Respir J, 2007, 30:467-471.
14. 杨玉书, 刘士林.吸烟与肺部感染在慢性阻塞性肺部疾病中的发病机理探讨.临床肺科杂志, 2009, 14:796-797.
15. Adair-Kirk TL, Atkinson JJ, Senior RM.Smoke particulates stress lung cells.Nat Med, 2008, 14:1024-1025.
16. Mangan PR, Harrington IE, Quinn DB, et al.Transforming growth factor-beta induces development of the TH17 Lineage.Nature, 2006, 441:231-234.
17. 姜敏, 田戈, 王晶, 等.维药神香草对COPD小鼠Th1/Th2和Th17/Treg平衡的影响.世界科学技术-中医药现代化, 2013, 15:591-594.
18. 王胜军, 许化溪, 杨胜利.CD4⁺CD25⁺调节性T细胞.细胞生物学杂志, 2005, 27:61-65.
19. Li MO, Wan YY, Flavell RA.T cell-produced transforming growth factorβ1 controls T cell tolerance and regulates Th1-and Th17-cell differentiation.Immunity, 2007, 26:579-591.

Chinese Journal of Respiratory and Critical Care Medicine

Effects of Smoking on Th17/Treg T Cell Subsets and Cytokines Expression in Patients with Chronic Obstructive Pulmonary Disease in Stable Phase

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