The Effect and Mechanism of Naringenin on Production of Chemokines in Human Bronchial Epithelial Cells_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

MaoShan ,  ShiYing , GuWei

Department of Respiratory Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, 210006, China;

Corresponding author：

ShiYing, Email: sykxss@163.com

Keywords：

Naringenin; Nuclear factor kappa B; Chemokines

DOI：

10.7507/1671-6205.2015009

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Objective To investigate the effects of naringenin on the production of chemokines and its mechanism in human bronchial epithelial (HBE) cells. Methods HBE cells were divided into a control group, a TNF-αgroup, a low-dose naringenin group, a moderate-dose naringenin group and a high-dose naringenin group. The Naringenin groups were incubated with different doses of naringenin (10, 5 and 2.5μmol/L, respectively) for 2 h. Then the naringenin groups and the TNF-αgroup were incubated with TNF-α. After 24 h of incubation, the levels of eotaxin and RANTES were determined by ELISA method, and IκBαdegradtion was detected by Western blot method. After incubated with TNF-αfor 30 min, NF-κB DNA-binding activity was detected by EMSA method. Results Compared with the control group, the levels of eotaxin and RANTES were significantly increased in the HBE cells stimulated with TNF-α. Naringenin had inhibitory effects on the expression of these chemokines. Naringenin abolished IκBαdegradation and reduced the DNA-binding activity of NF-κB. Conclusion Naringenin may inhibit the production of chemokines through inhibiting NF-κB pathway.

Citation： MaoShan, ShiYing, GuWei. The Effect and Mechanism of Naringenin on Production of Chemokines in Human Bronchial Epithelial Cells. Chinese Journal of Respiratory and Critical Care Medicine, 2015, 14(1): 32-35. doi: 10.7507/1671-6205.2015009 Copy

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8.	Lebovic DI, Chao VA, Martini JF, et al.IL-1beta induction of RANTES (regulated upon activation, normal T cell expressed and secreted) chemokine gene expression in endometriotic stromal cells depends on a nuclear factor-kappaB site in the proximal promoter. J Clin Endocrinol Metab, 2001, 86:4759-4764.
9.	Huber MA, Denk A, Peter RU, et al.The IKK-2/Ikappa Balpha/NF-kappa B pathway plays a key role in the regulation of CCR3 and eotaxin-1 in fibroblasts.A critical link to dermatitis in Ikappa Balpha-deficient mice.J Biol Chem, 2002, 277:1268-1275.
10.	Barnes PJ.Transcription factors in airway diseases.Lab Invest, 2006, 86:867-872.
11.	Chen ZJ.Ubiquitin signalling in the NF-kappaB pathway.Nat Cell Biol, 2005, 7:758-765.

1. Adcock IM, Ford PA, Bhavsar P, et al.Steroid resistance in asthma:mechanisms and treatment options. Curr Allergy Asthma Rep, 2008, 8:171-178.
2. Hämäläinen M, Nieminen R, Vuorela P, et al.Anti-inflammatory effects of flavonoids:genistein, kaempferol, quercetin, and daidzein inhibit STAT-1 and NF-κB activations, whereas flavone, isorhamnetin, naringenin, and pelargonidin inhibit only NF-κB activation along with their inhibitory effect on iNOS expression and NO production in activated macrophages. Mediators Inflamm, 2007, 2007:45673.
3. Kanno S, Tomizawa A, Hiura T, et al.Inhibitory effects of naringenin on tumor growth in human cancer cell lines and sarcoma S-180-implanted mice.Biol Pharm Bull, 2005, 28:527-530.
4. Zielińska-Przyjemska M, Ignatowicz E.Citrus fruit flavonoids influence on neutrophil apoptosis and oxidative metabolism. Phytother Res, 2008, 22:1557-1562.
5. Hsiao YC, Kuo WH, Chen PN, et al.Flavanone and 2'-OH flavanone inhibit metastasis of lung cancer cells via down-regulation of proteinases activities and MAPK pathway. Chem Biol Interact, 2007, 167:193-206.
6. Shi Y, Dai J, Liu H, et al.Naringenin inhibits allergen-induced airway inflammation and airway responsiveness and inhibits NF-κB activity in a murine model of asthma.Can J Physiol Phamacol, 2009, 87:729-735.
7. Ahn KS, Aggarwal BB.Transcription factor NF-kappaB:a sensor for smoke and stress signals.Ann N Y Acad Sci, 2005, 1056:218-233.
8. Lebovic DI, Chao VA, Martini JF, et al.IL-1beta induction of RANTES (regulated upon activation, normal T cell expressed and secreted) chemokine gene expression in endometriotic stromal cells depends on a nuclear factor-kappaB site in the proximal promoter. J Clin Endocrinol Metab, 2001, 86:4759-4764.
9. Huber MA, Denk A, Peter RU, et al.The IKK-2/Ikappa Balpha/NF-kappa B pathway plays a key role in the regulation of CCR3 and eotaxin-1 in fibroblasts.A critical link to dermatitis in Ikappa Balpha-deficient mice.J Biol Chem, 2002, 277:1268-1275.
10. Barnes PJ.Transcription factors in airway diseases.Lab Invest, 2006, 86:867-872.
11. Chen ZJ.Ubiquitin signalling in the NF-kappaB pathway.Nat Cell Biol, 2005, 7:758-765.

Chinese Journal of Respiratory and Critical Care Medicine

The Effect and Mechanism of Naringenin on Production of Chemokines in Human Bronchial Epithelial Cells

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