The Significance of Platelet Mitochondrial Function in Monitoring Severity of Sepsis_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

LiFen ¹ , YangHong ¹ , ChenHui ² ,  ChenZhongqing ²

1. Intensive Care Unit, The Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong, 510630, China;
2. ;

Corresponding author：

ChenZhongqing, Email: zhongqingchen2008@163.com

Keywords：

Sepsis; Mitochondria; Adenosine triphosphate; Platelet

DOI：

10.7507/1671-6205.2015012

Video：

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Objective To explore the relationship between mitochondrial function and the severity of sepsis by detecting the platelet mitochondrial permeability transition pore, transmembrane potential and adenosine triphosphate (ATP) levels in peripheral blood. Methods According to random number table, 40 male SD rats were randomly divided into three sepsis model groups (group A, B and C) and a sham group (group D). The rats in the model groups received cecal ligation and puncture (CLP) treatment with different percent of ligated length in total length of the cecum (10% in group A, 30% in group B and 50% in group C, respectively). Twenty-four hours later, peripheral blood was collected for TNF-α, IL-1βand IL-6 levels determination, also the mitochondrial permeability transition pore, transmembrane potential and ATP content were tested in the isolated platelet. One-way ANOVA test was used to determine the relevance between above indices and the severity of sepsis. Meanwhile, 29 patients with sepsis were enrolled for clinical study. After APACHEⅡscoring, platelet samples of peripheral blood in the patients were collected for mitochondrial function determination. The relationship between mitonchondrial function and APACHEⅡscore was analyzed by Spearman method. Results Calcein fluorescence, membrane potential and ATP synthesis in platelet mitochondria of the rat sepsis model were gradually decreased with the increased severity of CLP, and the difference among these groups were all statistically significant (all P < 0.05). In clinical specimens, APACHEⅡscore was negatively correlated with ATP level of platelet mitochondria(r=-0.895, P < 0.05). Conclusion Mitochondrial function of platelet in peripheral blood can be used as an effective indicator for the severity of sepsis.

Citation： LiFen, YangHong, ChenHui, ChenZhongqing. The Significance of Platelet Mitochondrial Function in Monitoring Severity of Sepsis. Chinese Journal of Respiratory and Critical Care Medicine, 2015, 14(1): 48-51. doi: 10.7507/1671-6205.2015012 Copy

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2.	Singer M.Mitochondrial function in sepsis:acute phase versus multiple organ failure.Crit Care Med, 2007, 35(9 Suppl):S441-S448.
3.	Rocha M, Herance R, Rovira S, et al.Mitochondrial dysfunction and antioxidant therapy in sepsis.Infect Disord Drug Targets, 2012, 12:161-178.
4.	Rittirsch D, Huber-Lang MS, Flierl MA, et al.Immunodesign of experimental sepsis by cecal ligation and puncture.Nat Protoc, 2009, 4:31-36.
5.	Levy MM, Fink MP, Marshall JC, et al.2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions Conference.Crit Care Med, 2003, 31:1250-1256.
6.	Levy RJ.Mitochondrial dysfunction, bioenergetic impairment, and metabolic down-regulation in sepsis.Shock, 2007, 28:24-28.
7.	Yorns WR Jr, Hardison HH.Mitochondrial dysfunction in migraine.Semin Pediatr Neurol, 2013, 20:188-193.
8.	Boisrame-Helms J, Kremer H, Schini-Kerth V, et al.Endothelial dysfunction in sepsis.Curr Vasc Pharmacol, 2013, 11:150-160.
9.	党晓燕, 秦勇, 丁新爱, 等.NT-proBNP、血小板及胆固醇水平与脓毒症预后的相关性.临床误诊误治, 2013, 26:80-83.
10.	Fink MP.Bench-to-bedside review:High-mobility group box 1 and critical illness.Crit Care, 2007, 11:229.
11.	陈昌勤, 张召才, 严静.脓毒症和心肌线粒体损伤.中国危重病急救医学, 2007, 19:630-632.
12.	荣子杰, 杨联军, 朱立新, 等.氧化应激诱导髓核细胞凋亡中细胞内活性氧及线粒体膜电位的变化.实用医学杂志, 2014, 30:193-195.
13.	Brealey D, Brand M, Hargreav I, et al.Association between mitochondrial dysfunction and severity and outcome of septic shock.Lancet, 2002, 7:219-223.

1. Exline MC, Crouser ED.Mitochondrial dysfunction during sepsis:still more questions than answers.Crit Care Med, 2011, 39:1216-1223.
2. Singer M.Mitochondrial function in sepsis:acute phase versus multiple organ failure.Crit Care Med, 2007, 35(9 Suppl):S441-S448.
3. Rocha M, Herance R, Rovira S, et al.Mitochondrial dysfunction and antioxidant therapy in sepsis.Infect Disord Drug Targets, 2012, 12:161-178.
4. Rittirsch D, Huber-Lang MS, Flierl MA, et al.Immunodesign of experimental sepsis by cecal ligation and puncture.Nat Protoc, 2009, 4:31-36.
5. Levy MM, Fink MP, Marshall JC, et al.2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions Conference.Crit Care Med, 2003, 31:1250-1256.
6. Levy RJ.Mitochondrial dysfunction, bioenergetic impairment, and metabolic down-regulation in sepsis.Shock, 2007, 28:24-28.
7. Yorns WR Jr, Hardison HH.Mitochondrial dysfunction in migraine.Semin Pediatr Neurol, 2013, 20:188-193.
8. Boisrame-Helms J, Kremer H, Schini-Kerth V, et al.Endothelial dysfunction in sepsis.Curr Vasc Pharmacol, 2013, 11:150-160.
9. 党晓燕, 秦勇, 丁新爱, 等.NT-proBNP、血小板及胆固醇水平与脓毒症预后的相关性.临床误诊误治, 2013, 26:80-83.
10. Fink MP.Bench-to-bedside review:High-mobility group box 1 and critical illness.Crit Care, 2007, 11:229.
11. 陈昌勤, 张召才, 严静.脓毒症和心肌线粒体损伤.中国危重病急救医学, 2007, 19:630-632.
12. 荣子杰, 杨联军, 朱立新, 等.氧化应激诱导髓核细胞凋亡中细胞内活性氧及线粒体膜电位的变化.实用医学杂志, 2014, 30:193-195.
13. Brealey D, Brand M, Hargreav I, et al.Association between mitochondrial dysfunction and severity and outcome of septic shock.Lancet, 2002, 7:219-223.

Chinese Journal of Respiratory and Critical Care Medicine

The Significance of Platelet Mitochondrial Function in Monitoring Severity of Sepsis

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