Role of p38 MAPK Signaling Pathway in sTREM-1 Expression of RAW264.7 Cells Induced by Lipopolysaccharide_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

 DuJuan ¹ , GaoWeiliang ² , FengQingzhou ¹ , LiuXuan ¹

1. Intensive Care Unit, The Seventh People's Hospital of Shenzhen, Shenzhen, Guangdong, 518081, China;
2. ;

Corresponding author：

DuJuan, Email: 965662785@qq.com

Keywords：

p38 mitiogen activated protein kinase; Lipopolysaccharide; Soluble triggering receptor expressed on myeloid cell-1

DOI：

10.7507/1671-6205.2015041

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the role of the p38 MAPK signaling pathway in sTREM-1 expression of RAW264.7 cells induced by lipopolysaccharide (LPS). Methods Macrophage cell line RAW264.7 cells were cultured in vitro and induced with the same concentration of LPS at different time. The protein expressions of p38 MAPK and phosphorylation of p38 MAPK(p-p38 MAPK) were detected by Western blot. The mRNA expression of p38 MAPK was detected by RT-PCR. The level of sTREM-1 was detected by enzyme linked immunosorbent assay method.The RAW264.7 cells were treated by SB203580 at different concentration,the changes of above indexes were observed. Results The p-p38 MAPK and p38 MAPK mRNA could be inducted by LPS in a time-dependent manner. The p-p38 MAPK and p38 MAPK mRNA could be inhibited by SB203580. After SB203580 blocking p38 MAPK signal transduction pathway,the sTREM-1 expression was significantly inhibited in a dose dependent manner. Conclusion LPS can induce sTREM-1 expression in RAW264.7 cells by activating the p38 MAPK signaling pathway.

Citation： DuJuan, GaoWeiliang, FengQingzhou, LiuXuan. Role of p38 MAPK Signaling Pathway in sTREM-1 Expression of RAW264.7 Cells Induced by Lipopolysaccharide. Chinese Journal of Respiratory and Critical Care Medicine, 2015, 14(2): 161-164. doi: 10.7507/1671-6205.2015041 Copy

1.	Adly AA, Ismail EA, Andrawes NG, et al.Circulating soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) as diagnostic and prognostic marker in neonatal sepsis. Cytokine, 2014, 65:184-191.
2.	Ye W, Hu Y, Zhang R, et al.Diagnostic value of the soluble triggering receptor expressed on myeloid cells-1 in lower respiratory tract infections:a meta-analysis.Respirology,2014,19:501-507.
3.	Zaringhalam J, Akhtari Z, Eidi A, et al.Relationship between serum IL-10 level and p38MAPK enzyme activity on behavioral and cellular aspects of variation of hyperalgesia during different stages of arthritis in rats.Inflammopharmacology,2014,22:37-44.
4.	Bihari D, Prakash S, Bersten A.Low-dose vasopressin in addition to noradrenaline may lead to faster resolution of organ failure in patients with severe sepsis/septic shock.Anaesth Intensive Care, 2014,42:671-674.
5.	Ruiz-Pacheco JA, Vivanco-Cid H, Izaguirre-Hernández IY, et al.TREM-1 modulation during early stages of dengue virus infection.Immunol Lett,2014,158:183-188.
6.	Palmiere C, Bardy D, Mangin P, et al.Value of sTREM-1, procalcitonin and CRP as laboratory parameters for postmortem diagnosis of sepsis.J Infect,2013,67:545-555.
7.	Qian L, Weng XW, Chen W, et al.TREM-1 as a potential therapeutic target in neonatal sepsis.Int J Clin Exp Med,2014,7:1650-1658.
8.	Park EJ, Park SW, Kim HJ, et al.Dehydrocostuslactone inhibits LPS-induced inflammation by p38MAPK-dependent induction of hemeoxygenase-1 in vitro and improves survival of mice in CLP-induced sepsis in vivo.Int Immunopharmacol,2014,22:332-340.
9.	Liu XW, Ji EF, He P, et al.Protective effects of the p38MAPK inhibitor SB203580 on NMDA-induced injury in primary cerebral cortical neurons.Mol Med Rep,2014,10:1942-1948.
10.	Yurinskaya MM, Vinokurov MG, Grachev SV,et al.Actovegin reduces the hydrogen peroxide-induced cell apoptosis of SK-N-SH neuroblastoma by means of p38MAPK and PI-3K inhibition.Dokl Biol Sci,2014,456:215-217.
11.	Lim J, Lee KM, Shim J, et al. CD24 regulates stemness and the epithelial to mesenchymal transition through modulation of Notch1 mRNA stability by p38MAPK. Arch Biochem Biophys,2014,558:120-126.
12.	Li W, Huang H, Zhang Y, et al.Anti-inflammatory effect of tetrahydrocoptisine from Corydalis impatiens is a function of possible inhibition of TNF-α,IL-6 and NO production in lipopolysaccharide-stimulated peritoneal macrophages through inhibiting NF-κB activation and MAPK pathway.Eur J Pharmacol,2013,715:62-71.
13.	Liu WH, Chang LS.Caffeine induces matrix metalloproteinase-2 (MMP-2) and MMP-9 down-regulation in human leukemia U937 cells via Ca²⁺/ROS-mediated suppression of ERK/c-fos pathway and activation of p38 MAPK/c-jun pathway.J Cell Physiol,2010,224:775-785.
14.	Holen E, Espe M, Andersen SM, et al. A co-culture approach show that polyamine turnover is affected during inflammation in Atlantic salmon immune and liver cells and that arginine and LPS exerts opposite effects on p38MAPK signaling.Fish Shellfish Immunol.2014,37:286-298.
15.	Huang C, Xie M, He X, et al. Activity of sputum p38 MAPK is correlated with airway inflammation and reduced FEV₁ in COPD patients.Med Sci Monit,2013,19:1229-1235.
16.	Nadeem A, Siddiqui N, Alharbi NO, et al.Acute glutathione depletion leads to enhancement of airway reactivity and inflammation via p38MAPK-iNOS pathway in allergic mice.Immunopharmacol,2014,22:222-229.
17.	Prüfer S,Weber M, Sasca D, et al.Distinct signaling cascades of TREM-1,TLR and NLR in neutrophils and monocytic cells.J Innate Immun,2014,6:339-352.

1. Adly AA, Ismail EA, Andrawes NG, et al.Circulating soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) as diagnostic and prognostic marker in neonatal sepsis. Cytokine, 2014, 65:184-191.
2. Ye W, Hu Y, Zhang R, et al.Diagnostic value of the soluble triggering receptor expressed on myeloid cells-1 in lower respiratory tract infections:a meta-analysis.Respirology,2014,19:501-507.
3. Zaringhalam J, Akhtari Z, Eidi A, et al.Relationship between serum IL-10 level and p38MAPK enzyme activity on behavioral and cellular aspects of variation of hyperalgesia during different stages of arthritis in rats.Inflammopharmacology,2014,22:37-44.
4. Bihari D, Prakash S, Bersten A.Low-dose vasopressin in addition to noradrenaline may lead to faster resolution of organ failure in patients with severe sepsis/septic shock.Anaesth Intensive Care, 2014,42:671-674.
5. Ruiz-Pacheco JA, Vivanco-Cid H, Izaguirre-Hernández IY, et al.TREM-1 modulation during early stages of dengue virus infection.Immunol Lett,2014,158:183-188.
6. Palmiere C, Bardy D, Mangin P, et al.Value of sTREM-1, procalcitonin and CRP as laboratory parameters for postmortem diagnosis of sepsis.J Infect,2013,67:545-555.
7. Qian L, Weng XW, Chen W, et al.TREM-1 as a potential therapeutic target in neonatal sepsis.Int J Clin Exp Med,2014,7:1650-1658.
8. Park EJ, Park SW, Kim HJ, et al.Dehydrocostuslactone inhibits LPS-induced inflammation by p38MAPK-dependent induction of hemeoxygenase-1 in vitro and improves survival of mice in CLP-induced sepsis in vivo.Int Immunopharmacol,2014,22:332-340.
9. Liu XW, Ji EF, He P, et al.Protective effects of the p38MAPK inhibitor SB203580 on NMDA-induced injury in primary cerebral cortical neurons.Mol Med Rep,2014,10:1942-1948.
10. Yurinskaya MM, Vinokurov MG, Grachev SV,et al.Actovegin reduces the hydrogen peroxide-induced cell apoptosis of SK-N-SH neuroblastoma by means of p38MAPK and PI-3K inhibition.Dokl Biol Sci,2014,456:215-217.
11. Lim J, Lee KM, Shim J, et al. CD24 regulates stemness and the epithelial to mesenchymal transition through modulation of Notch1 mRNA stability by p38MAPK. Arch Biochem Biophys,2014,558:120-126.
12. Li W, Huang H, Zhang Y, et al.Anti-inflammatory effect of tetrahydrocoptisine from Corydalis impatiens is a function of possible inhibition of TNF-α,IL-6 and NO production in lipopolysaccharide-stimulated peritoneal macrophages through inhibiting NF-κB activation and MAPK pathway.Eur J Pharmacol,2013,715:62-71.
13. Liu WH, Chang LS.Caffeine induces matrix metalloproteinase-2 (MMP-2) and MMP-9 down-regulation in human leukemia U937 cells via Ca²⁺/ROS-mediated suppression of ERK/c-fos pathway and activation of p38 MAPK/c-jun pathway.J Cell Physiol,2010,224:775-785.
14. Holen E, Espe M, Andersen SM, et al. A co-culture approach show that polyamine turnover is affected during inflammation in Atlantic salmon immune and liver cells and that arginine and LPS exerts opposite effects on p38MAPK signaling.Fish Shellfish Immunol.2014,37:286-298.
15. Huang C, Xie M, He X, et al. Activity of sputum p38 MAPK is correlated with airway inflammation and reduced FEV₁ in COPD patients.Med Sci Monit,2013,19:1229-1235.
16. Nadeem A, Siddiqui N, Alharbi NO, et al.Acute glutathione depletion leads to enhancement of airway reactivity and inflammation via p38MAPK-iNOS pathway in allergic mice.Immunopharmacol,2014,22:222-229.
17. Prüfer S,Weber M, Sasca D, et al.Distinct signaling cascades of TREM-1,TLR and NLR in neutrophils and monocytic cells.J Innate Immun,2014,6:339-352.

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Role of p38 MAPK Signaling Pathway in sTREM-1 Expression of RAW264.7 Cells Induced by Lipopolysaccharide

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