The Establishment of Gestational Diabetes and Its Effects on Lung Development_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

NiJuan , LinYuangui , WuLan , HuangWei ,  JiangXiaoqin

Department of Anesthesiology, West China Second Hospital, Sichuan University, Chengdu, Sichuan, 610041, China;

Corresponding author：

JiangXiaoqin, Email: 1598862657@qq.com

Keywords：

Gestational diabetes; Development of lung structure; Streptozotocin; Cell proliferation; Cell apoptosis

DOI：

10.7507/1671-6205.2015048

Video：

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Objective To establish a model of fetal hyperglycemia and explore the effects of hyperglycemia on alveolar cell apoptosis,proliferation and the development of lung structure in neonatal rats. Methods Forty SD pregnant rats were randomly divided into a hyperglycemia group (STZ group) and a normal pregnancy group (N group)(n=20 in each group). The rats in STZ group and N group were intraperitoneally injected streptozotocin(STZ,40 mg/kg) or the same bulk of citrate buffer respectively at the 4.5 days after gestation. Blood glucose concentration of the pregnant rats was measured before injection,at the 6.5,11.5,16.5 and 20.5 days after gestation,respectively. The weight,survival rate,lung weight,septal thickness,radical alveolar count,alveolar cell apoptosis and proliferation of neonatal rats were recorded after birth immediately (D0) and at 7 days (D7). Results Compared with N group,the blood glucose level increased after intraperitoneal injection of STZ in STZ group (P<0.05). The Survival rate of STZ group was lower than that of N group at D0 and D7 (P=0.00). The neonatal weight,lung weight,septal thickness of STZ group were lower compared with those of N group at D0(P<0.05). However,there was no significant difference between two groups in radical alveolar count. The alveolar apoptosis index of STZ group was higher than that of N group at D0 [(11.8±1.1)% vs. (3.4±0.7)%,P=0.00]. Alveolar cell proliferation significantly increased in STZ group compared with N group at D0 and D7 (P<0.05). Conclusions The fetal hyperglycemia model is successfully established by intraperitoneal injection of STZ 40 mg/kg. Fetal hyperglycemia can increase mortality,alveolar cell proliferation and apoptosis,and affect the development of lung structure of neonatal rats.

Citation： NiJuan, LinYuangui, WuLan, HuangWei, JiangXiaoqin. The Establishment of Gestational Diabetes and Its Effects on Lung Development. Chinese Journal of Respiratory and Critical Care Medicine, 2015, 14(2): 190-193. doi: 10.7507/1671-6205.2015048 Copy

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2.	Sukanya S,Bay BH,Tay SS,et al.Frontiers in research on maternal diabetes-induced neural tube defects:past, present and future.World J Diabetes,2012,3:196-200.
3.	Mendoza Martínez Tde J, Morales Morales MP, Jiménez Perea Mde L, et al. Fetal lung maturity delayed on diabetic pregnancies.Ginecol Obstet Mex,2005,73:183-193.
4.	Weindling AM.Offspring of diabetic pregnancy:short-term outcomes.Semin Fetal Neonatal Med,2009,14:111-118.
5.	Chappell JJ,Wang X,Loeken M.Diabetes and apoptosis:neural crest cells and neural tube. Apoptosis,2009,14:1472-1483.
6.	Jiang B,Kumar SD,Loh WT,et al.Global gene expression analysis of cranial neural tubes in embryos of diabetic mice.J Neurosci Res,2008,86:3481-3493.
7.	Gäreskog M,Cederberg J,Eriksson U,et al.Maternal diabetes in vivo and high glucose concentration in vitro increases apoptosis in rat embryos.Reprod Toxicol,2007,23:63-74.
8.	李鑫,罗淑静,张凯,等.链脲佐菌素诱导孕鼠高血糖子鼠高出生体质量模型的建立及孕鼠高血糖对子鼠远期生长代谢的影响.中华妇产科杂志,2012,47:769-776.
9.	Kiss AC, Woodside B, Felício LF, et al.Impact of maternal mild hyperglycemia on maternal care and offspring development and behavior of Wistar rats.Physiol Behav,2012,107:292-300.
10.	Jian Yun X, Zhaoxia L, Yun C, et al. Changes in maternal glucose metabolism after the administration of dexamethasone for fetal lung development. Int J Endocrinol,2012,2012:652806.

1. Yang PX, Li XZ, Xu C, et al. Maternal hyperglycemia activates an ASK1-FoxO3a-caspase 8 pathway that leads to embryonic neural tube defects.Sci Signal,2013,6:74.
2. Sukanya S,Bay BH,Tay SS,et al.Frontiers in research on maternal diabetes-induced neural tube defects:past, present and future.World J Diabetes,2012,3:196-200.
3. Mendoza Martínez Tde J, Morales Morales MP, Jiménez Perea Mde L, et al. Fetal lung maturity delayed on diabetic pregnancies.Ginecol Obstet Mex,2005,73:183-193.
4. Weindling AM.Offspring of diabetic pregnancy:short-term outcomes.Semin Fetal Neonatal Med,2009,14:111-118.
5. Chappell JJ,Wang X,Loeken M.Diabetes and apoptosis:neural crest cells and neural tube. Apoptosis,2009,14:1472-1483.
6. Jiang B,Kumar SD,Loh WT,et al.Global gene expression analysis of cranial neural tubes in embryos of diabetic mice.J Neurosci Res,2008,86:3481-3493.
7. Gäreskog M,Cederberg J,Eriksson U,et al.Maternal diabetes in vivo and high glucose concentration in vitro increases apoptosis in rat embryos.Reprod Toxicol,2007,23:63-74.
8. 李鑫,罗淑静,张凯,等.链脲佐菌素诱导孕鼠高血糖子鼠高出生体质量模型的建立及孕鼠高血糖对子鼠远期生长代谢的影响.中华妇产科杂志,2012,47:769-776.
9. Kiss AC, Woodside B, Felício LF, et al.Impact of maternal mild hyperglycemia on maternal care and offspring development and behavior of Wistar rats.Physiol Behav,2012,107:292-300.
10. Jian Yun X, Zhaoxia L, Yun C, et al. Changes in maternal glucose metabolism after the administration of dexamethasone for fetal lung development. Int J Endocrinol,2012,2012:652806.

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