Bleomycin-induced Pulmonary Fibrosis in Mice Model: Analysis of Difference in Gene Expression Profile_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

XiangWeihong ¹ ,  WangLifeng ² , MiaoYunbo ² ,  FuEnqing ¹ , JinFaguang ¹

1. Departmat of Respiratory and Critical Care Medicine, Tandu Hospital, The Fourth Military Medical University, Xi'an, Shanxi, 710038, China;
2. ;

Corresponding author：

WangLifeng, Email: lfwang@fmmu.edu.cn; FuEnqing, Email: fuenqing@sina.com

Keywords：

Bioinformatics; Pulmonary fibrosis; Gene profiles; BRB-Array tools

DOI：

10.7507/1671-6205.2015061

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the molecular pathogenesis of pulmonary fibrosis induced by bleomycin in a murine model,and provide novel insights for clinical diagnosis and treatment. Methods From Gene Expression Omnibus,we downloaded microarray data extracted from experiments of bleomycin induced pulmonary fibrosis in wild-type mice. With BRB-Array Tools,differentially expressed genes at different time points during disease development were screened,selected and analyzed by DAVID software. Results BRB array analysis identified 45101 differentially expressed genes. After induction by bleomycin on 7^th day,1164 genes and 735 genes were significantly up-regulated and down-regulated (P<0.05,fold change>2),respectively. On 14^th day,731 genes and 390 genes were significantly up-regulated and down-regulated (P<0.05,fold change>2),respectively. DAVID analysis revealed that the up-regulated genes were significantly enriched in cell cycle,p53 signaling and chemokine signaling pathway,damaging reaction and collagen metabolism gene sets. While the down-regulated genes were enriched in the drug metabolism pathway gene set. Conclusions Bioinformatics methodologies are able to efficiently analyze microarray data and extract its underlying information,provide novel insights for major molecular events and shift of cell signaling pathway during pulmonary fibrosis progression,and furthermore,finding molecular markers for early diagnosis and therapeutic targets.

Citation： XiangWeihong, WangLifeng, MiaoYunbo, FuEnqing, JinFaguang. Bleomycin-induced Pulmonary Fibrosis in Mice Model: Analysis of Difference in Gene Expression Profile. Chinese Journal of Respiratory and Critical Care Medicine, 2015, 14(3): 242-249. doi: 10.7507/1671-6205.2015061 Copy

1.	Strieter RM,Mehrad B. New mechanisms of pulmonary fibrosis. Chest,2009,136:1364-1370.
2.	Barkauskas CE,Noble PW. Cellular mechanisms of tissue fibrosis. 7. New insights into the cellular mechanisms of pulmonary fibrosis. Am J Physiol Cell Physiol,2014,306:C987-C996.
3.	Fernandez IE,Eickelberg O. New cellular and molecular mechanisms of lung injury and fibrosis in idiopathic pulmonary fibrosis. Lancet,2012,380:680-688.
4.	弓孟春,张博,徐作军.特发性肺纤维化发病率和患病率及临床病程的人群研究.中华结核和呼吸杂志,2010,33:306.
5.	Renzoni E,Srihari V,Sestini P. Pathogenesis of idiopathic pulmonary fibrosis:review of recent findings. F1000Prime Rep,2014,6:69.
6.	Spagnolo P,Rossi G,Cavazza A. Pathogenesis of idiopathic pulmonary fibrosis and its clinical implications. Expert Rev Clin Immunol,2014,10:1005-1017.
7.	Williamson JD,Sadofsky LR,Hart SP. The pathogenesis of bleomycin-induced lung injury in animals and its applicability to human idiopathic pulmonary fibrosis. Exp Lung Res,2015,41:57-73.
8.	Simon R,Lam A,Li MC,et al. Analysis of gene expression data using BRB-ArrayTools. Cancer Inform,2007,3:11-17.
9.	Kaminski N,Rosas IO. Gene expression profiling as a window into idiopathic pulmonary fibrosis pathogenesis:can we identify the right target genes? Proc Am Thorac Soc,2006,3:339-344.
10.	Oga T,Matsuoka T,Yao C,et al. Prostaglandin F(2alpha) receptor signaling facilitates bleomycin-induced pulmonary fibrosis independently of transforming growth factor-beta. Nat Med,2009,15:1426-1430.
11.	Ley B,Brown KK,Collard HR. Molecular biomarkers in idiopathic pulmonary fibrosis. Am J Physiol Lung Cell Mol Physiol,2014,307:L681-L691.
12.	Peng R,Sridhar S,Tyagi G,et al. Bleomycin induces molecular changes directly relevant to idiopathic pulmonary fibrosis:a model for "active" disease. PLoS One,2013,8:e59348.
13.	高建,刘干,李俊.肺成纤维细胞在肺纤维化进程中的作用.中国药理学通报,2010,26:1125-1128.
14.	高蔚,张德平,华云峰.姜黄素对大鼠肺成纤维细胞增殖和凋亡的影响.实用医学杂志,2008,24:2393-2396.
15.	孙燕川.沙利度胺对博莱霉素致大鼠肺纤维化的干预作用及机制.学位论文,河北医科大学,2011,49.
16.	Lilja-Maula L,Syrja P,Laurila HP,et al. Comparative study of transforming growth factor-beta signalling and regulatory molecules in human and canine idiopathic pulmonary fibrosis. J Comp Pathol,2014,150:399-407.
17.	赵永强,楚海燕,滕礼,等.博莱霉素诱导肺纤维化小鼠模型的建立及特征.疑难病杂志,2013,12:705-708.

1. Strieter RM,Mehrad B. New mechanisms of pulmonary fibrosis. Chest,2009,136:1364-1370.
2. Barkauskas CE,Noble PW. Cellular mechanisms of tissue fibrosis. 7. New insights into the cellular mechanisms of pulmonary fibrosis. Am J Physiol Cell Physiol,2014,306:C987-C996.
3. Fernandez IE,Eickelberg O. New cellular and molecular mechanisms of lung injury and fibrosis in idiopathic pulmonary fibrosis. Lancet,2012,380:680-688.
4. 弓孟春,张博,徐作军.特发性肺纤维化发病率和患病率及临床病程的人群研究.中华结核和呼吸杂志,2010,33:306.
5. Renzoni E,Srihari V,Sestini P. Pathogenesis of idiopathic pulmonary fibrosis:review of recent findings. F1000Prime Rep,2014,6:69.
6. Spagnolo P,Rossi G,Cavazza A. Pathogenesis of idiopathic pulmonary fibrosis and its clinical implications. Expert Rev Clin Immunol,2014,10:1005-1017.
7. Williamson JD,Sadofsky LR,Hart SP. The pathogenesis of bleomycin-induced lung injury in animals and its applicability to human idiopathic pulmonary fibrosis. Exp Lung Res,2015,41:57-73.
8. Simon R,Lam A,Li MC,et al. Analysis of gene expression data using BRB-ArrayTools. Cancer Inform,2007,3:11-17.
9. Kaminski N,Rosas IO. Gene expression profiling as a window into idiopathic pulmonary fibrosis pathogenesis:can we identify the right target genes? Proc Am Thorac Soc,2006,3:339-344.
10. Oga T,Matsuoka T,Yao C,et al. Prostaglandin F(2alpha) receptor signaling facilitates bleomycin-induced pulmonary fibrosis independently of transforming growth factor-beta. Nat Med,2009,15:1426-1430.
11. Ley B,Brown KK,Collard HR. Molecular biomarkers in idiopathic pulmonary fibrosis. Am J Physiol Lung Cell Mol Physiol,2014,307:L681-L691.
12. Peng R,Sridhar S,Tyagi G,et al. Bleomycin induces molecular changes directly relevant to idiopathic pulmonary fibrosis:a model for "active" disease. PLoS One,2013,8:e59348.
13. 高建,刘干,李俊.肺成纤维细胞在肺纤维化进程中的作用.中国药理学通报,2010,26:1125-1128.
14. 高蔚,张德平,华云峰.姜黄素对大鼠肺成纤维细胞增殖和凋亡的影响.实用医学杂志,2008,24:2393-2396.
15. 孙燕川.沙利度胺对博莱霉素致大鼠肺纤维化的干预作用及机制.学位论文,河北医科大学,2011,49.
16. Lilja-Maula L,Syrja P,Laurila HP,et al. Comparative study of transforming growth factor-beta signalling and regulatory molecules in human and canine idiopathic pulmonary fibrosis. J Comp Pathol,2014,150:399-407.
17. 赵永强,楚海燕,滕礼,等.博莱霉素诱导肺纤维化小鼠模型的建立及特征.疑难病杂志,2013,12:705-708.

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Bleomycin-induced Pulmonary Fibrosis in Mice Model: Analysis of Difference in Gene Expression Profile

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