The Expression and Significance of Senescence Marker Protein-30 in COPD Patients_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

GaoWei , WangYujie , JiangXinyu ,  YaoXin

Department of Respiratory Medicine, Jiangsu Province Hospital, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210029, China;

Corresponding author：

YaoXin, Email: yaoxin@njmu.edu.cn

Keywords：

Senescence marker protein-30; Alveolar macrophage; Chronic obstructive pulmonary disease

DOI：

10.7507/1671-6205.2015085

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To explore the expression of senescence marker protein-30 (SMP-30) in human lung tissues and the significance in the pathogenesis of chronic obstructive pulmonary disease (COPD). Methods Lung tissue specimens ( > 5 cm away from cancerous tissues) obtained by surgery resection in 20 subjects with solitary peripheral carcinoma in Jiangsu Province Hospital were investagted. The subjects were divided into three groups according to lung function and smoking history, ie. a COPD group (6 cases), a healthy smoking group (7 cases) and a healthy control group (7 cases). Immunohistochemistry and Western blot were used to determine the distribution and expression of SMP-30 in human lung tissues. Results SMP-30 protein mainly expressed in the cytoplasm of alveolar macrophages (AM). The numbers of AM and SMP-30-positive AM were significantly increased in the COPD group. Western blot analysis confirmed a significant increase in SMP-30 expression in the healthy smokers compared with the non-smokers (2.16±0.23 vs. 1.10±0.14, P < 0.01) and further enhanced in the patients with COPD compared with the healthy smoking subjects (4.62±0.97 vs. 2.16±0.23, P < 0.05). The levels of the protein in different groups were: COPD group > smoking group > control group with significant difference. Conclusion These results suggest that SMP-30 expression may be involved in the mechanism of prolonged survival and the increase in number of AM and may be involved in the pathogenesis of COPD.

Citation： GaoWei, WangYujie, JiangXinyu, YaoXin. The Expression and Significance of Senescence Marker Protein-30 in COPD Patients. Chinese Journal of Respiratory and Critical Care Medicine, 2015, 14(4): 341-344. doi: 10.7507/1671-6205.2015085 Copy

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5.	周素芳, 罗国容.衰老标记蛋白-30及其研究进展.科学技术与工程, 2004, 4:725-731.
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8.	Russell RE, Thorley A, Culpitt SV, et al.Alveolar macrophage mediated elastolysis:roles of matrix metalloproteinases, cysteine and serine proteases.Am J Physiol Lung Cell Mol Physiol, 2002, 283:L867-L873.
9.	Tetley TD.Macrophages and the pathogenesis of COPD.Chest, 2002, 121:156S-159S.
10.	Tomita K, Caramori G, Lim S, et al.Increased p21(CIP1/WAF1) and B cell lymphoma leukemia-x(L) expression and reduced apoptosis in alveolar macrophages from smokers.Am J Respir Crit Care Med, 2002, 166:724-731.
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13.	Sato T, Seyama K, Sato Y, et al.Senescence marker protein-30 protects mice lungs from oxidative stress, aging, and smoking.Am J Respir Crit Care Med, 2006, 174:530-537.
14.	Koike K, Kondo Y, Sekiya M, et al.Complete lack of vitamin C intake generates pulmonary emphysema in senescence marker protein-30 knockout mice.Am J Physiol Lung Cell Mol Physiol, 2010, 298:L784-L792.
15.	Siganaki M, Koutsopoulos AV, Neofytou E, et al.Deregulation of apoptosis mediators' p53 and bcl2 in lung tissue of COPD patients.Respir Res, 2010, 11:46.
16.	Kojima J, Araya J, Hara H, et al.Apoptosis inhibitor of macrophage(AIM) expression in alveolar macrophages in COPD. Respir Res, 2013, 14:30.
17.	Machiya J, Shibata Y, Yamauchi K, et al.Enhanced expression of MafB inhibits macrophage apoptosis induced by cigarette smoke exposure. Am J Respir Cell Mol Biol, 2007, 36:418-426.

1. Vestbo J, Hurd SS, Agusi AG, et al.Global strategy for the diagnosis management, and prevention of chronic obstructive pulmonary disease:GOLD executive summary.Am J Respir Crit Care Med, 2013, 187:347-365.
2. Brusselle GG, Joos GF, Bracke KR.New insights into the immunology of chronic obstructive pulmonary disease.Lancet, 2011, 378:1015-1026.
3. Vlahos R, Bozinovski S, Chan SP, et al.Neutralizing granulocyte/macrophage colony-stimulating factor inhibits cigarette smokeinduced lung inflammation.Am J Respir Crit Care Med, 2010, 182:34-40.
4. Fujita T, Shirasawa T, Uchida K, et al.Gene regulation of senescence marker protein-30(SMP30):coordinated up-regulation with tissue maturation and gradual down-regulation with aging.Mech Ageing Dev, 1996, 87:219-229.
5. 周素芳, 罗国容.衰老标记蛋白-30及其研究进展.科学技术与工程, 2004, 4:725-731.
6. Barnes PJ.Macrophages as orchestrators of COPD.COPD, 2004, 1:59-70.
7. Retamales I, Elliott WM, Meshi B, et al.Amplification of inflammation in emphysema and its association with latent adenoviral infection.Am J Respir Crit Care Med, 2001, 164:469-473.
8. Russell RE, Thorley A, Culpitt SV, et al.Alveolar macrophage mediated elastolysis:roles of matrix metalloproteinases, cysteine and serine proteases.Am J Physiol Lung Cell Mol Physiol, 2002, 283:L867-L873.
9. Tetley TD.Macrophages and the pathogenesis of COPD.Chest, 2002, 121:156S-159S.
10. Tomita K, Caramori G, Lim S, et al.Increased p21(CIP1/WAF1) and B cell lymphoma leukemia-x(L) expression and reduced apoptosis in alveolar macrophages from smokers.Am J Respir Crit Care Med, 2002, 166:724-731.
11. Mercado N, Ito K, Barnes PJ.Accelerated ageing of the lung in COPD:new concepts. Thorax, 2015, 70:482-489.
12. Mori T, Ishigami A, Seyama K, et al.Senescence marker protein-30 knockout mouse as a novel murine model of senile lung.Pathol Int, 2004, 54:167-173.
13. Sato T, Seyama K, Sato Y, et al.Senescence marker protein-30 protects mice lungs from oxidative stress, aging, and smoking.Am J Respir Crit Care Med, 2006, 174:530-537.
14. Koike K, Kondo Y, Sekiya M, et al.Complete lack of vitamin C intake generates pulmonary emphysema in senescence marker protein-30 knockout mice.Am J Physiol Lung Cell Mol Physiol, 2010, 298:L784-L792.
15. Siganaki M, Koutsopoulos AV, Neofytou E, et al.Deregulation of apoptosis mediators' p53 and bcl2 in lung tissue of COPD patients.Respir Res, 2010, 11:46.
16. Kojima J, Araya J, Hara H, et al.Apoptosis inhibitor of macrophage(AIM) expression in alveolar macrophages in COPD. Respir Res, 2013, 14:30.
17. Machiya J, Shibata Y, Yamauchi K, et al.Enhanced expression of MafB inhibits macrophage apoptosis induced by cigarette smoke exposure. Am J Respir Cell Mol Biol, 2007, 36:418-426.

Chinese Journal of Respiratory and Critical Care Medicine

The Expression and Significance of Senescence Marker Protein-30 in COPD Patients

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