The Difference in Expression of Programmed Death-1 Ligand on Monocytes between Mild and Severe Septic mice_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

LiuMinlong ¹ , WangGuoen ¹ ,  ZhangJun ²

1. Intensive Care Unit, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shanxi, 710004, China;
2. ;

Corresponding author：

ZhangJun, Email: jun3z@163.com

Keywords：

Sepsis; Programmed death-1 ligand; Monocytes

DOI：

10.7507/1671-6205.2015089

Video：

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Objective To explore the expression of programmed death-1 ligand (PD-L1) on peripheral blood monocytes in septic mice, and analyze the difference between the mild and severe septic mice. Methods Firstly, thirty C57 mice were randomly divided into a sham group, a mild sepsis group and a severe sepsis group. Sepsis model was induced by cecal ligation and puncture (CLP). The severity of sepsis was distinguished by length of cecum ligated. Survival rate was recorded after CLP and compared between three groups. Then sixty C57 mice were randomly divided into a sham group, a mild sepsis group and a severe sepsis group. Peripheral blood was obtained at 6 h and 24 h to detect PD-L1 expression on monocytes in peripheral blood by flow cytometry. Tumor necrosis factor-α(TNF-α) and interleukin-10 (IL-10) in serum were detected by enzyme-linked immunosorbent assay. Results The survival rate in the mild sepsis group was higher than that in the severe sepsis group. TNF-αand IL-10 levels in the mild and severe sepsis groups were higher than those in the sham group at 6 h and 24 h (P < 0.01). PD-L1 expression on monocytes in the mild and severe sepsis groups was higher than that in the sham group at 6 h and 24 h (P < 0.05). The expression of PD-L1 in the severe sepsis group was higher than that in the mild sepsis group, however, there was no statistical difference between two groups (P > 0.05). Conclusions PD-L1 expression on monocytes is increased in septic mice. PD-L1 expression tended to increase in severe sepsis compared with the mild sepsis.

Citation： LiuMinlong, WangGuoen, ZhangJun. The Difference in Expression of Programmed Death-1 Ligand on Monocytes between Mild and Severe Septic mice. Chinese Journal of Respiratory and Critical Care Medicine, 2015, 14(4): 358-361. doi: 10.7507/1671-6205.2015089 Copy

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1. 肖永红, 沈萍, 魏泽庆, 等.Mohnarin 2010年度全国细菌耐药监测.中华医院感染学杂志, 2011, 21:4896-4902.
2. Hotchkiss RS, Monneret G, Payen D.Sepsis-induced immunosuppression:from cellar dysfunction to immunotherapy.Nat Rev Immunol, 2013, 13:862-874.
3. Okazaki T, Honjo T.PD-1 and PD-1 ligands:from discovery to clinical application.Int Immunol, 2007, 19:813-824.
4. 郭国宁, 谢谆怡, 陈永文.共抑制分子PD-1的研究进展.免疫学杂志, 2010, 26:639-642.
5. Powles T, Eder JP, Fine GD, et al.MPDL3280A(anti-PD-L1) treatment leads to clinical activity in metastatic bladder cancer.Nature, 2014, 515:558-562.
6. Said EA, Dupuy FP, Trautmann L, et al.Programmed death-1-induced interleukin-10 production by monocytes impairs CD4⁺ T cell activation during HIV infection.Nat Med, 2010, 16:452-459.
7. Huang X, Chen Y, Chung CS, et al.Identification of B7-H1 as a novel mediator of the innate immune/proinflammatory response as well as a possible myeloid cell prognostic biomarker in sepsis.J Immunol, 2014, 192:1091-1099.
8. 刘茜, 陆江阳, 王晓虹, 等.MODS小鼠脾脏树突细胞PD-1、PD-L1的表达变化及其意义.解放军医学杂志, 2010, 35:17-19.
9. Zhang Y, Li J, Lou J, et, al.Upregulation of programmed death-1 on T cells and programmed death ligand-1 on monocytes in septic shock patients.Crit Care, 2011, 15:R70.
10. Guignant C, Lepape A, Huang X, et al.Programmed death-1 levels correlate with increased mortality, nosocomial infection and immune dysfunctions in septic shock patients.Crit Care, 2011, 15:R99.
11. Rittirsch D, Huber-Lang MS, Flierl MA, et al.Immunodesign of experimental sepsis by cecal ligation and puncture.Nat Protoc, 2009, 4:31-36.
12. Singleton KD, Wischmeyer PE.Distance of cecum ligated influences mortality, tumor necrosis factor-alpha and interleukin-6 expression following cecal ligation and puncture in the rat.Eur Surg Res, 2003, 35:486-491.

Chinese Journal of Respiratory and Critical Care Medicine

The Difference in Expression of Programmed Death-1 Ligand on Monocytes between Mild and Severe Septic mice

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