Clinical significance of IL-17, IL-10, and 8-iso-PG in exhaled breath condensate of patients with acute exacerbation of chronic obstructive pulmonary disease_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

 YAN Binlin ¹ , GU Weili ² , DU Juan ¹ , ZHOU Huidong ¹

1. Department of Respiratory Medicine, The Seventh People’s Hospital of Shenzhen, Shenzhen, Guangdong 518081, P.R.China;
2. Department of Respiratory Medicine, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510120, P.R.China;

Corresponding author：

YAN Binlin, Email: yblelite@163.com

Keywords：

Acute exacerbation of chronic obstructive pulmonary disease; Exhaled breath condensate; Interleukin-17; Interleukin-10; 8-Isoprestane

DOI：

10.7507/1671-6205.201609016

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Objective To investigate the changes and clinical significance of cytokines and inflammatory species in exhaled breath condensate (EBC) in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD). Methods Thirty AECOPD patients admitted in the Department of Respiratory Medicine from March 2015 to August 2016 (smokers and passive smokers) and 21 healthy volunteers (non-smokers) were recruited in this prospective study. General information and EBC were collected from each subject. The concentrations of interleukin-17 (IL-17), IL-10, and 8-isoprestane (8-iso-PG) in EBC were measured by enzyme-linked immunosorbent assay, meanwhile lung function test was performed in the AECOPD patients. Results Both IL-17 (ng/L) and 8-iso-PG (ng/L) levels increased significantly in the AECOPD patients before and after treatment compared with the healthy controls (10.74±1.02 and 5.65±0.88 vs. 3.36±0.61, 12.35±2.25 and 9.65±1.22 vs. 6.93±1.15, P<0.05). However, IL-10 level significantly decreased in the AECOPD patients before and after treatment compared with the healthy controls (1.68±0.17 and 2.59±0.31 vs. 2.85±0.43, P<0.05). Both IL-17 and 8-iso-PG levels in the AECOPD patients were significantly lower after treatment than those before treatment (5.65±0.88 vs. 10.74±1.02, 9.65±1.22 vs. 12.35±2.25, P<0.05), but IL-10 level were significantly higher aftertreatment than those before treatment (2.59±0.31 vs. 1.68±0.17, P<0.05). FEV₁, FVC, and FEV₁%pred improved significantly after treatment (P<0.01). FEV₁, FEV₁/FVC and FEV₁%pred were not significantly correlated with IL-17, IL-10 or 8-is-PG levels. Conclusion IL-17, IL-10 and 8-iso-PG may be involved in the pathogenesis of COPD, and may be important biomarkers in monitoring airway inflammation and oxide stress during the treatment of AECOPD patients.

Citation： YAN Binlin, GU Weili, DU Juan, ZHOU Huidong. Clinical significance of IL-17, IL-10, and 8-iso-PG in exhaled breath condensate of patients with acute exacerbation of chronic obstructive pulmonary disease. Chinese Journal of Respiratory and Critical Care Medicine, 2017, 16(2): 142-146. doi: 10.7507/1671-6205.201609016 Copy

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2.	中华医学会呼吸病学分会慢性阻塞性肺疾病学组. 慢性阻塞性肺疾病诊治指南(2013 年修订版). 中华结核和呼吸杂志, 2013, 36(4): 255-264.
3.	Nefedov VB, Shergina EA, Popova LA. Evaluation of the course of chronic obstructive lung diseases according to the classifications of the European Respiratory Society and the Global Initiative on Chronic Obstructive Lung Disease. Probl Tuberk Bolezn Legk, 2006, (2): 44-47.
4.	刘霞, 许文兵, 刘亚男, 等. 呼出气一氧化氮对成人支气管哮喘治疗的指导作用. 中国呼吸与危重监护杂志, 2014, 13(2): 120-124.
5.	Zhao YF, Jiang YP, Zhou LF, et al. The value of assessment tests in patients with acute exacerbation of chronic obstructive pulmonary disease. Am J Med Sci, 2014, 347(5): 393-399.
6.	Di Stefano A, Caramori G, Gnemmi I, et al. T helper type 17-related cytokine expression is increased in the bronchial mucosa of stable chronic obstructive pulmonary disease patients. Clin Exp Immunol, 2009, 157(2): 316-324.
7.	刘仁杰, 万毅新, 王晓平, 等. 慢性阻塞性肺疾病患者血清 IL-17、IL-23 水平及临床意义. 重庆医科大学学报, 2011, 36(8): 976-978.
8.	万鹏程, 关国跃. IL-17 在慢性阻塞性肺疾病发病机制中的作用. 临床肺科杂志, 2011, 16(7): 1086-1088.
9.	何小双, 许西琳, 刘冬, 等. 慢性阻塞性肺疾病患者呼出气冷凝液中白介素 8、白介素 17 水平及其与第 1 秒用力呼气末容积占预计值百分比的关系研究. 中国全科医学, 2016, 19(1): 63-67.
10.	Tone M, Powell MJ, Tone Y, et al. IL-10 gene expression is controlled by the transcription factors Sp1 and Sp3. J Immunity, 2010, 165(1): 286-291.
11.	郝璐, 崔丽英. 支气管哮喘和 COPD 患者血清 IL-10 水平的临床研究. 临床肺科杂志, 2012, 17(2): 228-230.
12.	Macdonald M, Korman T, King P, et al. Exacerbation phenotyping in chronic obstructive pulmonary disease. Respirology, 2013, 18(8): 1280 -1281.
13.	朱峥, 周奇兴, 邹盈, 等. 白介素 8 和白介素 10 在老年慢性阻塞性肺疾病诱导痰中的表达以及药物治疗对其的影响. 国际呼吸杂志, 2012, 32(7): 493-496.
14.	李小莉, 黄平, 杜秀芳. 慢性阻塞性肺疾病治疗期间呼出气冷凝液中 8-异前列腺素、IL-6、IL-10 的变化及与气道炎症的相关性研究. 中国呼吸与危重监护杂志, 2014, 13(5): 445-448.
15.	Davidson WJ, Verity WS, Traves SL, et al. Effect of incremental exercise on airway and systemic inflammation in patients with COPD. J Appl Physiol, 2012, 112(12): 2049-2056.
16.	Montuschi P, Barnes PJ, Roberts LJ. Isoprostanes: markers and mediators of oxidative stress. FASEB J, 2004, 18(5): 1791-1800.
17.	Crooks SW, Bayley DL, Hill SL, et al. Bronchial inflammation in acute bacterial exacerbations of chronic bronchitis: the role of leukotriene B4. Eur Respir J, 2000, 15(2): 274-280.
18.	Kostikas K, Papatheodorou G, Psathakis K, et al. Oxidative stress in expired breath condensate of patients with COPD. Chest, 2003, 124(4): 1373-1380.
19.	Rahman I, Kelly F. Biomarkers in breath condensate: a promising new non-invasive technique in free radical research. Free Radic Res, 2003, 37(12): 1253-1266.
20.	陈金亮, 陈建荣, 蔡映云, 等. COPD 患者呼出气冷凝液中 8-异前列腺素的检测及其意义. 临床肺科杂志, 2011, 16(3): 341-343.
21.	郭凯, 殷少军. 慢性阻塞性肺疾病急性加重期相关生物标志物及其诊断意义. 中华诊断学电子杂志, 2015, 3(2): 133-137.
22.	姚苏梅, 陈金亮, 吕学东, 等. 社区获得性肺炎患者呼出气冷凝液和血清中 8-异前列腺素检测的临床意义. 检验医学与临床, 2016, 13(8): 1020-1024.
23.	李小莉, 黄平, 杜秀芳. COPD 治疗期间呼出气冷凝液中细胞因子的变化及意义. 临床肺科杂志, 2014, 19(7): 1222-1224.

1. Melsen WG, Rovers MM, Koeman M, et al. Estimating the attributable mortality of ventilator-associated pneumonia from randomized prvention studies. Crit Care, 2011, 39(12): 2736-2742.
2. 中华医学会呼吸病学分会慢性阻塞性肺疾病学组. 慢性阻塞性肺疾病诊治指南(2013 年修订版). 中华结核和呼吸杂志, 2013, 36(4): 255-264.
3. Nefedov VB, Shergina EA, Popova LA. Evaluation of the course of chronic obstructive lung diseases according to the classifications of the European Respiratory Society and the Global Initiative on Chronic Obstructive Lung Disease. Probl Tuberk Bolezn Legk, 2006, (2): 44-47.
4. 刘霞, 许文兵, 刘亚男, 等. 呼出气一氧化氮对成人支气管哮喘治疗的指导作用. 中国呼吸与危重监护杂志, 2014, 13(2): 120-124.
5. Zhao YF, Jiang YP, Zhou LF, et al. The value of assessment tests in patients with acute exacerbation of chronic obstructive pulmonary disease. Am J Med Sci, 2014, 347(5): 393-399.
6. Di Stefano A, Caramori G, Gnemmi I, et al. T helper type 17-related cytokine expression is increased in the bronchial mucosa of stable chronic obstructive pulmonary disease patients. Clin Exp Immunol, 2009, 157(2): 316-324.
7. 刘仁杰, 万毅新, 王晓平, 等. 慢性阻塞性肺疾病患者血清 IL-17、IL-23 水平及临床意义. 重庆医科大学学报, 2011, 36(8): 976-978.
8. 万鹏程, 关国跃. IL-17 在慢性阻塞性肺疾病发病机制中的作用. 临床肺科杂志, 2011, 16(7): 1086-1088.
9. 何小双, 许西琳, 刘冬, 等. 慢性阻塞性肺疾病患者呼出气冷凝液中白介素 8、白介素 17 水平及其与第 1 秒用力呼气末容积占预计值百分比的关系研究. 中国全科医学, 2016, 19(1): 63-67.
10. Tone M, Powell MJ, Tone Y, et al. IL-10 gene expression is controlled by the transcription factors Sp1 and Sp3. J Immunity, 2010, 165(1): 286-291.
11. 郝璐, 崔丽英. 支气管哮喘和 COPD 患者血清 IL-10 水平的临床研究. 临床肺科杂志, 2012, 17(2): 228-230.
12. Macdonald M, Korman T, King P, et al. Exacerbation phenotyping in chronic obstructive pulmonary disease. Respirology, 2013, 18(8): 1280 -1281.
13. 朱峥, 周奇兴, 邹盈, 等. 白介素 8 和白介素 10 在老年慢性阻塞性肺疾病诱导痰中的表达以及药物治疗对其的影响. 国际呼吸杂志, 2012, 32(7): 493-496.
14. 李小莉, 黄平, 杜秀芳. 慢性阻塞性肺疾病治疗期间呼出气冷凝液中 8-异前列腺素、IL-6、IL-10 的变化及与气道炎症的相关性研究. 中国呼吸与危重监护杂志, 2014, 13(5): 445-448.
15. Davidson WJ, Verity WS, Traves SL, et al. Effect of incremental exercise on airway and systemic inflammation in patients with COPD. J Appl Physiol, 2012, 112(12): 2049-2056.
16. Montuschi P, Barnes PJ, Roberts LJ. Isoprostanes: markers and mediators of oxidative stress. FASEB J, 2004, 18(5): 1791-1800.
17. Crooks SW, Bayley DL, Hill SL, et al. Bronchial inflammation in acute bacterial exacerbations of chronic bronchitis: the role of leukotriene B4. Eur Respir J, 2000, 15(2): 274-280.
18. Kostikas K, Papatheodorou G, Psathakis K, et al. Oxidative stress in expired breath condensate of patients with COPD. Chest, 2003, 124(4): 1373-1380.
19. Rahman I, Kelly F. Biomarkers in breath condensate: a promising new non-invasive technique in free radical research. Free Radic Res, 2003, 37(12): 1253-1266.
20. 陈金亮, 陈建荣, 蔡映云, 等. COPD 患者呼出气冷凝液中 8-异前列腺素的检测及其意义. 临床肺科杂志, 2011, 16(3): 341-343.
21. 郭凯, 殷少军. 慢性阻塞性肺疾病急性加重期相关生物标志物及其诊断意义. 中华诊断学电子杂志, 2015, 3(2): 133-137.
22. 姚苏梅, 陈金亮, 吕学东, 等. 社区获得性肺炎患者呼出气冷凝液和血清中 8-异前列腺素检测的临床意义. 检验医学与临床, 2016, 13(8): 1020-1024.
23. 李小莉, 黄平, 杜秀芳. COPD 治疗期间呼出气冷凝液中细胞因子的变化及意义. 临床肺科杂志, 2014, 19(7): 1222-1224.

Chinese Journal of Respiratory and Critical Care Medicine

Clinical significance of IL-17, IL-10, and 8-iso-PG in exhaled breath condensate of patients with acute exacerbation of chronic obstructive pulmonary disease

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