痰培养联合曲霉血清学GM试验对诊断侵袭性曲霉感染的临床价值研究_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

 刘利华 ¹ , 刘元元 ¹ , 高东田 ¹ , 李怀生 ²

1. ;
2. ;

Corresponding author：

刘利华, Email: 1340236848@qq.com

Keywords：

半乳甘露聚糖; 痰培养; 曲霉

DOI：

10.7507/1671-6205.2016138

Video：

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目的评价痰培养联合曲霉血清学半乳甘露聚糖(GM)试验对侵袭性曲霉感染的临床诊断价值。方法对济宁医学院附属医院2012年7月至2013年7月间疑似曲霉感染的216例住院患者进行痰培养和血清ELISA检测。以临床诊断为标准, 评估痰培养和GM试验及联合检测的敏感性、特异性、阳性预测值和阴性预测值, 采用χ²检验进行比较。应用受试者工作特征曲线(ROC曲线)比较不同检测的疾病识别能力。结果痰培养的敏感性和特异性为60.4%和69.9%, GM试验为94.3%和94.5%, 二者联合检测可将诊断的敏感性和特异性分别提高至98.1%和99.4%。联合检测的ROC曲线下面积(AUC)为0.960, 95%可信区间为0.929~0.992。结论痰真菌培养联合血清GM抗原检测能大幅提高曲霉感染病例的临床诊断效率。

Citation： 刘利华, 刘元元, 高东田, 李怀生. 痰培养联合曲霉血清学GM试验对诊断侵袭性曲霉感染的临床价值研究. Chinese Journal of Respiratory and Critical Care Medicine, 2016, 15(6): 607-609. doi: 10.7507/1671-6205.2016138 Copy

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4.	Brezillon C, Kroon PA, Faulds CB, et al. Novel ferulic acid esterases are induced by growth of Aspergillus niger on sugar-beet pulp. Appl Microbiol Biotechnol, 1996, 45:371-376.
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6.	Faulds CB, Williamson G. Release of ferulic acid from wheat bran by a ferulic acid esterase (FAE Ⅲ) from Aspergillus niger. Appl Microbiol Biotechnol, 1995, 43:1082-1087.
7.	Eraso F, Hartley RD. Monomeric and dimeric phenolic consituents of plant cell walls-possible factors influencing wall biodegradability. J Sci Food Agric, 1990, 51:163-170.
8.	Yu P, McKinnon JJ, Maenz DD, et al. The interactive effects of enriched sources of Aspergillus ferulic acid esterase and Trichoderma xylanase on the quantitative release of hydroxycinnamic acids from oat hulls. Can Ver J, 2002, 82:251-257.
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10.	Anderson G, McNellis L. Enzyme-linked antibodies:a laboratory introduction to the ELISA assay. J Chem Educ, 1998, 75:1275-1277.
11.	Scharf A, Kasel U, Wichmann G, et al. Performance of ELISA and PCR methods for the determination of allergens in food:an evaluation of six years of proficiency testing for Soy (Glycine max L.) and wheat gluten (Triticum aestivum L.). J Agric Food Chem, 2013, 61:10261-10272.

1. 杨莉.慢性阻塞性肺疾病急性加重期住院患者肺部真菌感染临床分析.中华医院感染学杂志, 2012, 22:5520-5522.
2. Goldman GH, Yu C, Wu HY, et al. Sanders MM, Differential poisoning of human and Aspergillus nidulans DNA topoisomerase I by bi-and terbenzimidazoles. Biochemistry, 1997, 36:6488-6494.
3. Yu P, McKinnon JJ, Maenz DD, et al. Enzymic release of reducing sugars from oat hulls by cellulase, as influenced by Aspergillus ferulic acid esterase and trichoderma xylanase. J Agric Food Chem, 2003, 51:218-223.
4. Brezillon C, Kroon PA, Faulds CB, et al. Novel ferulic acid esterases are induced by growth of Aspergillus niger on sugar-beet pulp. Appl Microbiol Biotechnol, 1996, 45:371-376.
5. Kroon PA, Faulds CB, Williamson G. Purification and characterization of a novel esterase induced by growth of Aspergillus niger on sugar-beet pulp. Biotechnol Appl Biochem, 1996, 23:255-262.
6. Faulds CB, Williamson G. Release of ferulic acid from wheat bran by a ferulic acid esterase (FAE Ⅲ) from Aspergillus niger. Appl Microbiol Biotechnol, 1995, 43:1082-1087.
7. Eraso F, Hartley RD. Monomeric and dimeric phenolic consituents of plant cell walls-possible factors influencing wall biodegradability. J Sci Food Agric, 1990, 51:163-170.
8. Yu P, McKinnon JJ, Maenz DD, et al. The interactive effects of enriched sources of Aspergillus ferulic acid esterase and Trichoderma xylanase on the quantitative release of hydroxycinnamic acids from oat hulls. Can Ver J, 2002, 82:251-257.
9. Brandon DL, Bates AH, Binder RG, et al. Analysis of fenbendazole residues in bovine milk by ELISA. J Agric Food Chem, 2002, 50:5791-5796.
10. Anderson G, McNellis L. Enzyme-linked antibodies:a laboratory introduction to the ELISA assay. J Chem Educ, 1998, 75:1275-1277.
11. Scharf A, Kasel U, Wichmann G, et al. Performance of ELISA and PCR methods for the determination of allergens in food:an evaluation of six years of proficiency testing for Soy (Glycine max L.) and wheat gluten (Triticum aestivum L.). J Agric Food Chem, 2013, 61:10261-10272.

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痰培养联合曲霉血清学GM试验对诊断侵袭性曲霉感染的临床价值研究

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