Expression of SIR2-related enzyme 1 in serum of patients with chronic obstructive pulmonary disease and its clinical significance_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

 GUO Guohua , XIAO Jianhong , SONG Bin , ZENG Linmiao

Department of Respiratory Medicine, Mindong Hospital of Fujian Medical University, Fuan, Fujian 355000, P. R. China;

Corresponding author：

GUO Guohua, Email: 277681575@qq.com

Keywords：

SIR2-related enzyme 1; Chronic obstructive pulmonary disease; Pulmonary function; Serum

DOI：

10.7507/1671-6205.201706042

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ObjectiveTo investigate the expression of SIR2-related enzyme 1 (SIRT1) in serum of patients with chronic obstructive pulmonary disease (COPD) and its clinical significance.MethodsForty patients with acute exacerbation of COPD (acute-COPD group) and 30 patients with stable COPD (stable-COPD group) were selected as study groups. Twenty healthy subjects were recruited as a control group. Serum SIRT1, nuclear factor (NF) -κB, and matrix metalloproteinases (MMP) -9 levels were detected by enzyme-linked immunosorbent assay and Western blot.ResultsCompared with the control group, the FEV₁%pred and FEV₁/FVC were significantly decreased in the acute COPD patients and the stable COPD patients (P<0.05). Compared with the stable-COPD group, FEV₁%pred and FEV₁/FVC were significantly decreased in the acute-COPD group (P<0.05). Compared with control group, the serum SIRT1 in the stable-COPD group and the acute-COPD group were significantly decreased, while NF-κB and MMP-9 were significantly increased (P<0.05). Compared with the stable-COPD group, SIRT1 in the acute-COPD group was significantly decreased, while NF-κB and MMP-9 were significantly increased (P<0.05). SIRT1 were positively correlated with FEV₁%pred (P<0.05) and FEV₁/FVC (P<0.05) in the COPD patients by Pearson linear correlation analysis. SIRT1 was negatively correlated with NF-κB (P<0.05) and MMP-9 (P<0.05).ConclusionIn COPD patients, decreased levels of serum SIRT1 is associates with decreased lung function, which may be used as a potential biomarker of COPD.

Citation： GUO Guohua, XIAO Jianhong, SONG Bin, ZENG Linmiao. Expression of SIR2-related enzyme 1 in serum of patients with chronic obstructive pulmonary disease and its clinical significance. Chinese Journal of Respiratory and Critical Care Medicine, 2018, 17(4): 336-340. doi: 10.7507/1671-6205.201706042 Copy

1.	Conti V, Corbi G, Simeon V, et al. Aging-related changes in oxidative stress response of human endothelial cells. Aging Clin Experim Res, 2015, 27(4): 1-7.
2.	中华医学会呼吸病学分会慢性阻塞性肺疾病学组. 慢性阻塞性肺疾病诊治指南 (2013 年修订版). 中国医学前沿杂志电子版, 2014, 6(2): 67-80.
3.	高薇. 醒脑静对慢阻肺急性加重期呼吸衰竭患者肺功能与血气分析的影响. 中医药导报, 2014(15): 51-53.
4.	张晓慧. 炎性细胞因子水平检测对慢性阻塞性肺疾病的临床意义. 海南医学院学报, 2016, 22(3): 240-242.
5.	Testa G, Cacciatore F, Bianco A, et al. Chronic obstructive pulmonary disease and long-term mortality in elderly subjects with chronic heart failure. Aging Clin Experim Res, 2017, 29(6): 1-8.
6.	Mercado N, Ito K, Barnes PJ. Accelerated ageing of the lung in COPD: new concepts. Thorax, 2015, 70(5): 482-489.
7.	李芳, 关文霞, 任飞, 等. Sirt-1 和 Hif-1α 在慢性阻塞性肺疾病患者外周血单个核细胞中的表达及其意义. 吉林大学学报 (医学版), 2015, 41(2): 356-361.
8.	Ondracek CR, Frappier V, Ringel AE, et al. Mutations that allow SIR2 orthologs to function in a NAD+-depleted environment. Cell Rep, 2017, 18(10): 2310-2319.
9.	Ganesan R, Hos NJ, Gutierrez S, et al. Salmonella typhimurium disrupts Sirt1/AMPK checkpoint control of mTOR to impair autophagy. Plos Pathogens, 2017, 13(2): e1006227.
10.	Emamgholipour S, Hosseinnezhad A, Sahraian MA, et al. Evidence for possible role of melatonin in reducing oxidative stress in multiple sclerosis through its effect on SIRT1 and antioxidant enzymes. Life Sci, 2016, 145(8): 34-41.
11.	Zhong Y, Chen AF, Zhao J, et al. Serum levels of cathepsin D, sirtuin1, and endothelial nitric oxide synthase are correlatively reduced in elderly healthy people. Aging Clin Experim Res, 2016, 28(4): 641-645.
12.	Wong SY, Tang BL. SIRT1 as a therapeutic target for Alzheimer’s disease. Rev Neurosci, 2016, 27(8): 813-825.
13.	Testa G, Staurenghi E, Zerbinati C, et al. Changes in brain oxysterols at different stages of Alzheimer’s disease: Their involvement in neuroinflammation. Redox Biol, 2016, 10(C): 24-33.
14.	Yanagisawa S, Papaioannou AI, Papaporfyriou A, et al. Decreased Serum Sirtuin-1 in COPD. Chest, 2017, 152(2): 343-352.
15.	Taka C, Hayashi R, Shimokawa K, et al. SIRT1 and FOXO1 mRNA expression in PBMC correlates to physical activity in COPD patients. Int J Chron Obstruct Pulmon Dis, 2017, 12(8): 3237-3244.
16.	伍造端, 邓立普. NF-κB 在慢性阻塞性肺疾病中的作用. 蛇志, 2015, 24(1): 60-61.
17.	Cui X, Chen Q, Dong Z, et al. Inactivation of Sirt1 in mouse livers protects against endotoxemic liver injury by acetylating and activating NF-κB. Cell Death Dis, 2016, 7(10): e2403.
18.	李敏, 王宇宏, 李巍. 基质金属蛋白酶 2, 9, 12 与慢性阻塞性肺疾病的临床研究. 中国医学创新, 2016, 13(2): 44-47.
19.	Nakamaru Y, Vuppusetty C, Wada H, et al. A protein deacetylase SIRT1 is a negative regulator of metalloproteinase-9. FASEB J, 2009, 23(9): 2810-2819.

1. Conti V, Corbi G, Simeon V, et al. Aging-related changes in oxidative stress response of human endothelial cells. Aging Clin Experim Res, 2015, 27(4): 1-7.
2. 中华医学会呼吸病学分会慢性阻塞性肺疾病学组. 慢性阻塞性肺疾病诊治指南 (2013 年修订版). 中国医学前沿杂志电子版, 2014, 6(2): 67-80.
3. 高薇. 醒脑静对慢阻肺急性加重期呼吸衰竭患者肺功能与血气分析的影响. 中医药导报, 2014(15): 51-53.
4. 张晓慧. 炎性细胞因子水平检测对慢性阻塞性肺疾病的临床意义. 海南医学院学报, 2016, 22(3): 240-242.
5. Testa G, Cacciatore F, Bianco A, et al. Chronic obstructive pulmonary disease and long-term mortality in elderly subjects with chronic heart failure. Aging Clin Experim Res, 2017, 29(6): 1-8.
6. Mercado N, Ito K, Barnes PJ. Accelerated ageing of the lung in COPD: new concepts. Thorax, 2015, 70(5): 482-489.
7. 李芳, 关文霞, 任飞, 等. Sirt-1 和 Hif-1α 在慢性阻塞性肺疾病患者外周血单个核细胞中的表达及其意义. 吉林大学学报 (医学版), 2015, 41(2): 356-361.
8. Ondracek CR, Frappier V, Ringel AE, et al. Mutations that allow SIR2 orthologs to function in a NAD+-depleted environment. Cell Rep, 2017, 18(10): 2310-2319.
9. Ganesan R, Hos NJ, Gutierrez S, et al. Salmonella typhimurium disrupts Sirt1/AMPK checkpoint control of mTOR to impair autophagy. Plos Pathogens, 2017, 13(2): e1006227.
10. Emamgholipour S, Hosseinnezhad A, Sahraian MA, et al. Evidence for possible role of melatonin in reducing oxidative stress in multiple sclerosis through its effect on SIRT1 and antioxidant enzymes. Life Sci, 2016, 145(8): 34-41.
11. Zhong Y, Chen AF, Zhao J, et al. Serum levels of cathepsin D, sirtuin1, and endothelial nitric oxide synthase are correlatively reduced in elderly healthy people. Aging Clin Experim Res, 2016, 28(4): 641-645.
12. Wong SY, Tang BL. SIRT1 as a therapeutic target for Alzheimer’s disease. Rev Neurosci, 2016, 27(8): 813-825.
13. Testa G, Staurenghi E, Zerbinati C, et al. Changes in brain oxysterols at different stages of Alzheimer’s disease: Their involvement in neuroinflammation. Redox Biol, 2016, 10(C): 24-33.
14. Yanagisawa S, Papaioannou AI, Papaporfyriou A, et al. Decreased Serum Sirtuin-1 in COPD. Chest, 2017, 152(2): 343-352.
15. Taka C, Hayashi R, Shimokawa K, et al. SIRT1 and FOXO1 mRNA expression in PBMC correlates to physical activity in COPD patients. Int J Chron Obstruct Pulmon Dis, 2017, 12(8): 3237-3244.
16. 伍造端, 邓立普. NF-κB 在慢性阻塞性肺疾病中的作用. 蛇志, 2015, 24(1): 60-61.
17. Cui X, Chen Q, Dong Z, et al. Inactivation of Sirt1 in mouse livers protects against endotoxemic liver injury by acetylating and activating NF-κB. Cell Death Dis, 2016, 7(10): e2403.
18. 李敏, 王宇宏, 李巍. 基质金属蛋白酶 2, 9, 12 与慢性阻塞性肺疾病的临床研究. 中国医学创新, 2016, 13(2): 44-47.
19. Nakamaru Y, Vuppusetty C, Wada H, et al. A protein deacetylase SIRT1 is a negative regulator of metalloproteinase-9. FASEB J, 2009, 23(9): 2810-2819.

Chinese Journal of Respiratory and Critical Care Medicine

Expression of SIR2-related enzyme 1 in serum of patients with chronic obstructive pulmonary disease and its clinical significance

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