The expression and function of Kv1.3 channel in peripheral blood T lymphocytes of patients with chronic obstructive pulmonary disease_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

SHI Ying ,  MAO Shan

Department of Respiratory Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu 210006, P. R. China;

Corresponding author：

MAO Shan, Email: maoshan68@163.com

Keywords：

Chronic obstructive pulmonary disease; Kv1.3 channel; T lymphocyte

DOI：

10.7507/1671-6205.201802029

Video：

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Objective To detect the expression and function of Kv1.3 channel in peripheral blood T lymphocytes of chronic obstructive pulmonary disease (COPD) patients, and to explore the possibility of Kv1.3 channel blockers in treating chronic airway inflammation in patients with COPD.Methods T lymphocytes were isolated from peripheral blood of COPD patients and healthy controls. Then the mRNA and protein levels of Kv1.3 were detected in T cells. The effects of Kv1.3 channel inhibitors ShK on T cell proliferation and the production of interleukin 2 were detected.Results The Kv1.3 level of peripheral blood T lymphocytes in patients with COPD was significantly higher than that of healthy controls. ShK significantly inhibited the proliferation and interleukin 2 production ability of T lymphocytes.Conclusions Kv1.3 may play important roles in inflammation in COPD. Selective blocking of Kv1.3 channels may be one of the future treatment for COPD.

Citation： SHI Ying, MAO Shan. The expression and function of Kv1.3 channel in peripheral blood T lymphocytes of patients with chronic obstructive pulmonary disease. Chinese Journal of Respiratory and Critical Care Medicine, 2019, 18(2): 120-123. doi: 10.7507/1671-6205.201802029 Copy

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10.	Kazama I, Maruyama Y, Murata Y. Suppressive effects of nonsteroidal anti-inflammatory drugs diclofenac sodium, salicylate and indomethacin on delayed rectifier K⁺ -channel currents in murine thymocytes. Immunopharmacol Immunotoxicol, 2012, 34(5): 874-878.
11.	Kazama I, Maruyama Y. Differential effects of clarithromycin and azithromycin on delayed rectifier K⁺-channel currents in murine thymocytes. Pharm Biol, 2013, 51(6): 760-765.
12.	Kazama I, Maruyama Y, Matsubara M. Benidipine persistently inhibits delayed rectifier K⁺-channel currents in murine thymocytes. Immunopharmacol Immunotoxicol, 2013, 35(1): 28-33.
13.	Kazama I, Baba A, Maruyama Y. HMG-CoA reductase inhibitors pravastatin, lovastatin and simvastatin suppress delayed rectifier K⁺-channel currents in murine thymocytes. Pharmacol Rep, 2014, 66(4): 712-717.
14.	Baba A, Tachi M, Maruyama Y, et al. Suppressive effects of diltiazem and verapamil on delayed rectifier K⁺-channel currents in murine thymocytes. Pharmacol Rep, 2015, 67(5): 959-964.

1. Barnes PJ, Shapiro SD, Pauwels RA. Chronic obstructive pulmonary disease: molecular and cellular mechanisms. Eur Respir J, 2003, 22(4): 672-688.
2. Hodge S, Hodge G, Nairn J, et al. Increased airway granzyme b and perforin in current and ex-smoking COPD subjects. COPD, 2006, 3(4): 179-187.
3. Pelaia G, Gallelli L, Vatrella A,et al. Potential role of potassium channel openers in the treatment of asthma and chronic obstructive pulmonary disease. Life Sci, 2002, 70(9): 977-990.
4. Kazama I. Physiological significance of delayed rectifier K⁺ channels (Kv1.3) expressed in T lymphocytes and their pathological significance in chronic kidney disease. J Physiol Sci, 2015, 65(1): 25-35.
5. Hu L, Pennington M, Jiang Q, et al. Characterization of the functional properties of the voltage-gated potassium channel Kv1.3 in human CD4⁺T lymphocytes. J Immunol, 2007, 179(7): 4563-4570.
6. 中华医学会呼吸病分会慢性阻塞性肺部疾病学组. 慢性阻塞性肺疾病诊治指南(2013 年修订版). 中华结核和呼吸杂志, 2013, 36(4): 255-264.
7. Domagała-Kulawik J, Hoser G, Dąbrowska M, et al. CD4+/CD25+ cells in systemic inflammation in COPD. Scand J Immunol, 2011, 73(1): 59-65.
8. Wulff H, Calabresi PA, Allie R, et al. The voltage-gated Kv1.3 K⁺ channel in effector memory T cells as new target for MS. J Clin Invest, 2003, 111(11): 1703-1713.
9. Cassell D, Forman J. Linked recognition of helper and cytotoxic antigenic determinants for the generation of cytotoxic T lymphocytes. Ann NY Acad Sci, 1988, 532(1): 51-60.
10. Kazama I, Maruyama Y, Murata Y. Suppressive effects of nonsteroidal anti-inflammatory drugs diclofenac sodium, salicylate and indomethacin on delayed rectifier K⁺ -channel currents in murine thymocytes. Immunopharmacol Immunotoxicol, 2012, 34(5): 874-878.
11. Kazama I, Maruyama Y. Differential effects of clarithromycin and azithromycin on delayed rectifier K⁺-channel currents in murine thymocytes. Pharm Biol, 2013, 51(6): 760-765.
12. Kazama I, Maruyama Y, Matsubara M. Benidipine persistently inhibits delayed rectifier K⁺-channel currents in murine thymocytes. Immunopharmacol Immunotoxicol, 2013, 35(1): 28-33.
13. Kazama I, Baba A, Maruyama Y. HMG-CoA reductase inhibitors pravastatin, lovastatin and simvastatin suppress delayed rectifier K⁺-channel currents in murine thymocytes. Pharmacol Rep, 2014, 66(4): 712-717.
14. Baba A, Tachi M, Maruyama Y, et al. Suppressive effects of diltiazem and verapamil on delayed rectifier K⁺-channel currents in murine thymocytes. Pharmacol Rep, 2015, 67(5): 959-964.

Chinese Journal of Respiratory and Critical Care Medicine

The expression and function of Kv1.3 channel in peripheral blood T lymphocytes of patients with chronic obstructive pulmonary disease

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