Effect of aquaporin 1 on the migration of endothelial progenitor cells_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

GE Haiyan ¹ , ZHU Huili ¹ ,  XIA Shijin ² , SONG Yuanlin ³ ,  BAI Chunxue ³

1. Department of Pulmonary Medicine, Huadong Hospital, Fudan University, Shanghai 200040, P. R. China;
2. Department of Geriatrics, Huadong Hospital, Fudan University, Shanghai 200040, P. R. China;
3. Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, P. R. China;

Corresponding author：

XIA Shijin, Email: xiashijinhd@163.com

Keywords：

Aquaporin 1; Endothelial progenitor cells; Cell migration; Acute lung injury

DOI：

10.7507/1671-6205.201804037

Video：

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ObjectiveTo observe the effects of aquaporin 1 (AQP1) on the proliferation and migration of endothelial progenitor-endothelial progenitor cells (EPC).MethodsBone marrow cells of AQP1 wild-type (WT) (n=6) and knockout-type (KO) mice (n=6) were isolated and differentiated into EPC in vitro. Immunofluorescence was used to detect cell surface antigens to identify EPC. Live cell kinetic imaging and quantification technology, transwell migration assays, as well as scratch test were used to compare the function of EPC between AQP1 WT and KO mice.ResultsEPC culture showed that cells were initially suspended and gradually adhered to typical mesenchymal stem cells within 7 days. After cultured on special medium for endothelial cells they were adhered and differentiated, and fusiform or polygonal, paving stone-like EPC were observed around 14 days. When cultured by special medium of EPC, CD133 and CD31 were positively detected after 7 days, and CD34 and Flk-1 were positively detected after 14 days. Positive expression of AQP1 was only detected in EPC of AQP1 WT mice. Functional studies of EPC revealed there was no significant difference in the proliferation of EPC between AQP1 WT and KO group mice. Transwell assay showed that EPC migration ability of AQP1 KO mice was significantly weaker than that of WT mice. The scratch healing ability of EPC in AQP1 KO mice was significantly lower than that of WT mice.ConclusionsEPC initially shows the characteristics of stem cells and with the prolongation of culture time, EPC gradually shows the characteristics of endothelial cells. AQP1 affects the EPC migration rather than proliferation.

Citation： GE Haiyan, ZHU Huili, XIA Shijin, SONG Yuanlin, BAI Chunxue. Effect of aquaporin 1 on the migration of endothelial progenitor cells. Chinese Journal of Respiratory and Critical Care Medicine, 2018, 17(3): 290-295. doi: 10.7507/1671-6205.201804037 Copy

1.	Herrero R, Sanchez G, Lorente JA. New insights into the mechanisms of pulmonary edema in acute lung injury. Annals of translational medicine, 2018, 6(2): 32.
2.	Rubenfeld GD, Caldwell E, Peabody E, et al. Incidence and outcomes of acute lung injury. The New England journal of medicine, 2005, 353(16): 1685-1693.
3.	Bhattacharya J, Matthay MA. Regulation and repair of the alveolar-capillary barrier in acute lung injury. Annual review of physiology, 2013, 75: 593-615.
4.	Strumwasser A, Bhargava A, Victorino GP. Attenuation of Endothelial Phosphatidylserine Exposure Decreases Ischemia-reperfusion INDUCED Changes in Microvascular Permeability. The journal of trauma and acute care surgery, 2018.
5.	Garbuzova-Davis S, Haller E, Lin R, et al. Intravenously Transplanted Human Bone Marrow Endothelial Progenitor Cells Engraft Within Brain Capillaries, Preserve Mitochondrial Morphology, and Display Pinocytotic Activity Toward Blood-Brain Barrier Repair in Ischemic Stroke Rats. Stem cells, 2017, 35(5): 1246-1258.
6.	Meamar R, Nikyar H, Dehghani L, et al. The role of endothelial progenitor cells in transient ischemic attack patients for future cerebrovascular events. Journal of research in medical sciences: the official journal of Isfahan University of Medical Sciences, 2016, 21: 47.
7.	Lin CY, Tzeng HE, Li TM, et al. WISP-3 inhibition of miR-452 promotes VEGF-A expression in chondrosarcoma cells and induces endothelial progenitor cells angiogenesis. Oncotarget, 2017, 8(24): 39571-39581.
8.	Bai C, Fukuda N, Song Y, et al. Lung fluid transport in aquaporin-1 and aquaporin-4 knockout mice. The Journal of clinical investigation, 1999, 103(4): 555-561.
9.	Song Y, Yang B, Matthay MA, et al. Role of aquaporin water channels in pleural fluid dynamics. American journal of physiology Cell physiology, 2000, 279(6): C1744-1750.
10.	Loreto C, Reggio E. Aquaporin and vascular diseases. Current neuropharmacology, 2010, 8(2): 105-111.
11.	Rutkovskiy A, Bliksoen M, Hillestad V, et al. Aquaporin-1 in cardiac endothelial cells is downregulated in ischemia, hypoxia and cardioplegia. Journal of molecular and cellular cardiology, 2013, 56: 22-33.
12.	Ran X, Wang H, Chen Y, et al. Aquaporin-1 expression and angiogenesis in rabbit chronic myocardial ischemia is decreased by acetazolamide. Heart and vessels, 2010, 25(3): 237-247.
13.	Gill SE, Yamashita CM, Veldhuizen RA. Lung remodeling associated with recovery from acute lung injury. Cell and tissue research, 2017, 367(3): 495-509.
14.	Wang T, Gross C, Desai AA, et al. Endothelial cell signaling and ventilator-induced lung injury: molecular mechanisms, genomic analyses, and therapeutic targets. American journal of physiology Lung cellular and molecular physiology, 2017, 312(4): L452-L476.
15.	Ge H, Zhu H, Xu N, et al. Increased Lung Ischemia-Reperfusion Injury in Aquaporin 1-Null Mice Is Mediated via Decreased Hypoxia-Inducible Factor 2alpha Stability. American journal of respiratory cell and molecular biology, 2016, 54(6): 882-891.
16.	Saadoun S, Papadopoulos MC, Hara-Chikuma M, et al. Impairment of angiogenesis and cell migration by targeted aquaporin-1 gene disruption. Nature, 2005, 434(7034): 786-792.
17.	Mun GI, Jang SI, Boo YC. Laminar shear stress induces the expression of aquaporin 1 in endothelial cells involved in wound healing. Biochemical and biophysical research communications, 2013, 430(2): 554-559.
18.	Yokomori H, Oda M, Yoshimura K, et al. Aquaporin-1 associated with hepatic arterial capillary proliferation on hepatic sinusoid in human cirrhotic liver. Liver international: official journal of the International Association for the Study of the Liver, 2011, 31(10): 1554-1564.
19.	Huebert RC, Vasdev MM, Shergill U, et al. Aquaporin-1 facilitates angiogenic invasion in the pathological neovasculature that accompanies cirrhosis. Hepatology, 2010, 52(1): 238-248.
20.	Zaccone V, Flore R, Santoro L, et al. Focus on biological identity of endothelial progenitors cells. European review for medical and pharmacological sciences, 2015, 19(21): 4047-4063.
21.	Huizer K, Mustafa DAM, Spelt JC, et al. Improving the characterization of endothelial progenitor cell subsets by an optimized FACS protocol. PloS one, 2017, 12(9): e0184895.

1. Herrero R, Sanchez G, Lorente JA. New insights into the mechanisms of pulmonary edema in acute lung injury. Annals of translational medicine, 2018, 6(2): 32.
2. Rubenfeld GD, Caldwell E, Peabody E, et al. Incidence and outcomes of acute lung injury. The New England journal of medicine, 2005, 353(16): 1685-1693.
3. Bhattacharya J, Matthay MA. Regulation and repair of the alveolar-capillary barrier in acute lung injury. Annual review of physiology, 2013, 75: 593-615.
4. Strumwasser A, Bhargava A, Victorino GP. Attenuation of Endothelial Phosphatidylserine Exposure Decreases Ischemia-reperfusion INDUCED Changes in Microvascular Permeability. The journal of trauma and acute care surgery, 2018.
5. Garbuzova-Davis S, Haller E, Lin R, et al. Intravenously Transplanted Human Bone Marrow Endothelial Progenitor Cells Engraft Within Brain Capillaries, Preserve Mitochondrial Morphology, and Display Pinocytotic Activity Toward Blood-Brain Barrier Repair in Ischemic Stroke Rats. Stem cells, 2017, 35(5): 1246-1258.
6. Meamar R, Nikyar H, Dehghani L, et al. The role of endothelial progenitor cells in transient ischemic attack patients for future cerebrovascular events. Journal of research in medical sciences: the official journal of Isfahan University of Medical Sciences, 2016, 21: 47.
7. Lin CY, Tzeng HE, Li TM, et al. WISP-3 inhibition of miR-452 promotes VEGF-A expression in chondrosarcoma cells and induces endothelial progenitor cells angiogenesis. Oncotarget, 2017, 8(24): 39571-39581.
8. Bai C, Fukuda N, Song Y, et al. Lung fluid transport in aquaporin-1 and aquaporin-4 knockout mice. The Journal of clinical investigation, 1999, 103(4): 555-561.
9. Song Y, Yang B, Matthay MA, et al. Role of aquaporin water channels in pleural fluid dynamics. American journal of physiology Cell physiology, 2000, 279(6): C1744-1750.
10. Loreto C, Reggio E. Aquaporin and vascular diseases. Current neuropharmacology, 2010, 8(2): 105-111.
11. Rutkovskiy A, Bliksoen M, Hillestad V, et al. Aquaporin-1 in cardiac endothelial cells is downregulated in ischemia, hypoxia and cardioplegia. Journal of molecular and cellular cardiology, 2013, 56: 22-33.
12. Ran X, Wang H, Chen Y, et al. Aquaporin-1 expression and angiogenesis in rabbit chronic myocardial ischemia is decreased by acetazolamide. Heart and vessels, 2010, 25(3): 237-247.
13. Gill SE, Yamashita CM, Veldhuizen RA. Lung remodeling associated with recovery from acute lung injury. Cell and tissue research, 2017, 367(3): 495-509.
14. Wang T, Gross C, Desai AA, et al. Endothelial cell signaling and ventilator-induced lung injury: molecular mechanisms, genomic analyses, and therapeutic targets. American journal of physiology Lung cellular and molecular physiology, 2017, 312(4): L452-L476.
15. Ge H, Zhu H, Xu N, et al. Increased Lung Ischemia-Reperfusion Injury in Aquaporin 1-Null Mice Is Mediated via Decreased Hypoxia-Inducible Factor 2alpha Stability. American journal of respiratory cell and molecular biology, 2016, 54(6): 882-891.
16. Saadoun S, Papadopoulos MC, Hara-Chikuma M, et al. Impairment of angiogenesis and cell migration by targeted aquaporin-1 gene disruption. Nature, 2005, 434(7034): 786-792.
17. Mun GI, Jang SI, Boo YC. Laminar shear stress induces the expression of aquaporin 1 in endothelial cells involved in wound healing. Biochemical and biophysical research communications, 2013, 430(2): 554-559.
18. Yokomori H, Oda M, Yoshimura K, et al. Aquaporin-1 associated with hepatic arterial capillary proliferation on hepatic sinusoid in human cirrhotic liver. Liver international: official journal of the International Association for the Study of the Liver, 2011, 31(10): 1554-1564.
19. Huebert RC, Vasdev MM, Shergill U, et al. Aquaporin-1 facilitates angiogenic invasion in the pathological neovasculature that accompanies cirrhosis. Hepatology, 2010, 52(1): 238-248.
20. Zaccone V, Flore R, Santoro L, et al. Focus on biological identity of endothelial progenitors cells. European review for medical and pharmacological sciences, 2015, 19(21): 4047-4063.
21. Huizer K, Mustafa DAM, Spelt JC, et al. Improving the characterization of endothelial progenitor cell subsets by an optimized FACS protocol. PloS one, 2017, 12(9): e0184895.

Chinese Journal of Respiratory and Critical Care Medicine

Effect of aquaporin 1 on the migration of endothelial progenitor cells

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