Effect of ampelopsin in mice with bleomycin-induced pulmonary fibrosis_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

HUANG Haitao ^1,2 , FANF Jing ¹ , ZENG Chunhui ¹ , YIN Xijing ¹ , HONG Zhengshan ¹ , TAN Liuping ¹ ,  YANG Ke ^1,2

1. College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, P. R. China;
2. Guangxi Engineering Technology Research Center of Advantage Chinese Patent Drug and Ethnic Drug Development, Nanning, Guangxi 530020, P. R. China;

Corresponding author：

YANG Ke, Email: kyang13481020102@126.com

Keywords：

Pulmonary fibrosis; Ampelopsin; Respiratory function; In vivo; Bleomycin

DOI：

10.7507/1671-6205.201903086

Video：

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Objective This study construct a pulmonary fibrosis model in vivo to study anti-pulmonary fibrosis effect of ampelopsis.Methods We constructed a pulmonary fibrosis model by bleomycin in BALB/c mice. The mice were divided by weight random number table into a blank control group, a model control group, a dexamethasone treatment group (intervened with dexamethasone in a dose of 2.5 mg/kg), and three ampelopsis treatment groups intervened with ampelopsis in dose of 200, 100, and 50 mg/kg, respectively. Bleomycin solution (3 mg/kg) was intratracheally injected respectively on 1^st and 14^th day, except the blank group. Twenty-eight days later, the relevant indicators were collected, including respiratory function (airway resistance, dynamic lung compliance, maximal ventilator volume), level of hydroxyproline and histopathological changes in the lungs.Results After 28 days, the model control group mice had severe respiratory resistance, dynamic lung compliance and maximal ventilator volume were decreased. The high dose ampelopsis treatment could enhance respiratory function (P<0.05). Lung coefficient was lower in the treatment groups than that in the model control group (P<0.05). The hydroxyproline of the treatment groups was less than that of the model control group (P<0.05). Histopathological examination showed that the degree of fibrosis increased in the model control group (P<0.05), but decreased in the treatment groups (P<0.05).Conclusion Ampelopsis can resist bleomycin-induced pulmonary fibrosis in mice, relieve the symptoms of respiratory failure, reduce the formation of collagen, and produce anti-pulmonary fibrosis effect.

Citation： HUANG Haitao, FANF Jing, ZENG Chunhui, YIN Xijing, HONG Zhengshan, TAN Liuping, YANG Ke. Effect of ampelopsin in mice with bleomycin-induced pulmonary fibrosis. Chinese Journal of Respiratory and Critical Care Medicine, 2020, 19(3): 270-275. doi: 10.7507/1671-6205.201903086 Copy

1.	黄海涛, 唐艺文, 杨柯. 肺纤维化信号调控通路的研究进展. 国际呼吸杂志, 2018, 38(4): 311-314.
2.	徐冰珠, 康建磊. 特发性肺纤维化治疗药物的研究进展. 现代药物与临床, 2015, 30(7): 897-902.
3.	Mouratis MA, Aidinis V. Modeling pulmonary fibrosis with bleomycin. Curr Opin Pulm Med, 2011, 17(5): 355-361.
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6.	谢郁峰, 陈卓瀚, 吕华冲. 响应面分析法优化藤茶中二氢杨梅素的提取工艺. 中国实验方剂学杂志, 2012, 18(17): 46-49.
7.	张友胜, 宁正祥, 杨书珍, 等. 显齿蛇葡萄中二氢杨梅树皮素的抗氧化作用及其机制(英文). 药学学报, 2003, 38(4): 241-244.
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12.	Tan WJ, Tan QY, Wang T, et al. Calpain 1 regulates TGF-β1-induced epithelial-mesenchymal transition in human lung epithelial cells via PI3K/Akt signaling pathway. Am J Transl Res, 2017, 9(3): 1402-1409.
13.	Moore BB, Hogaboam CM. Murine models of pulmonary fibrosis. Am J Physiol Lung Cell Mol Physiol, 2008, 294(2): L152-L160.
14.	刘虹, 李辙, 吕晓希, 等. 中和 IL-17A 活性改善肺纤维化小鼠肺功能. 航天医学与医学工程, 2013, 26(1): 38-42.
15.	中华医学会呼吸病学分会肺功能专业组. 肺功能检查指南(第二部分)——肺量计检查. 中华结核和呼吸杂志, 2014, 37(7): 481-486.
16.	刘加良, 施广懿. 脉冲振荡与通气肺功能二种检测方法比较. 浙江预防医学, 2002, 14(11): 6-8.
17.	鲁惠顺, 卫木根, 胡方. 潮气量与肺顺应性、呼气第一秒容量及压力—容量的相关性. 浙江临床医学, 2001, 3(11): 812-813.
18.	方贤磊, 李杨, 聂李亚, 等. 肺功能分析在放射性肺损伤防治药物药效评价中的作用. 军事医学, 2015, 39(7): 519-522.
19.	杨秀颖, 杜冠华. 组织羟脯氨酸含量微量测定方法及应用. 中国药理学通报, 2004, 20(7): 836-837.
20.	谢伟娜, 丁奇, 孙静, 等. 百部汤对博来霉素诱导小鼠肺纤维化的抑制作用. 中国药科大学学报, 2018, 49(4): 483-489.
21.	Chen C, Wang YY, Wang YX, et al. Gentiopicroside ameliorates bleomycin-induced pulmonary fibrosis in mice via inhibiting inflammatory and fibrotic process. Biochem Biophys Res Commun, 2018, 495(4): 2396-2403.
22.	林艺凯, 马爱平, 周伟跃, 等. mTOR 信号通路在博来霉素诱导小鼠肺纤维化中的作用机制研究. 中国呼吸与危重监护杂志, 2018, 17(2): 178-182.
23.	马爱平, 高云周, 兰文斌, 等. PI3K/Akt/HIF-1α 信号通路在博来霉素诱导小鼠肺纤维化中的作用机制研究. 中国呼吸与危重监护杂志, 2016, 15(1): 34-38.

1. 黄海涛, 唐艺文, 杨柯. 肺纤维化信号调控通路的研究进展. 国际呼吸杂志, 2018, 38(4): 311-314.
2. 徐冰珠, 康建磊. 特发性肺纤维化治疗药物的研究进展. 现代药物与临床, 2015, 30(7): 897-902.
3. Mouratis MA, Aidinis V. Modeling pulmonary fibrosis with bleomycin. Curr Opin Pulm Med, 2011, 17(5): 355-361.
4. 苏素娇. 显齿蛇葡萄品质评价及其相关药效学研究[D]. 福建中医药大学, 2014.
5. 中国科学院植物研究所. 中国高等植物图鉴(第二册)补编. 北京: 科学出版社, 1983, 349-356.
6. 谢郁峰, 陈卓瀚, 吕华冲. 响应面分析法优化藤茶中二氢杨梅素的提取工艺. 中国实验方剂学杂志, 2012, 18(17): 46-49.
7. 张友胜, 宁正祥, 杨书珍, 等. 显齿蛇葡萄中二氢杨梅树皮素的抗氧化作用及其机制(英文). 药学学报, 2003, 38(4): 241-244.
8. 阎莉, 卫智权, 郑作文, 等. 广西藤茶双氢杨梅树皮素对小鼠肝细胞凋亡的影响. 中国实验方剂学杂志, 2010, 16(16): 102-104.
9. Ashcroft T, Simpson JM, Timbrell V. Simple method of estimating severity of pulmonary fibrosis on a numerical scale. J Clin Pathol, 1988, 41(4): 467.
10. Wilson MS, Wynn TA. Pulmonary fibrosis: pathogenesis, etiology and regulation. Mucosal Immunol, 2009, 2(2): 103-121.
11. 邢小燕, 李光伟. 类固醇糖尿病——糖皮质激素治疗中必须警惕的不良反应. 中华风湿病学杂志, 2009, 13(12): 801-803.
12. Tan WJ, Tan QY, Wang T, et al. Calpain 1 regulates TGF-β1-induced epithelial-mesenchymal transition in human lung epithelial cells via PI3K/Akt signaling pathway. Am J Transl Res, 2017, 9(3): 1402-1409.
13. Moore BB, Hogaboam CM. Murine models of pulmonary fibrosis. Am J Physiol Lung Cell Mol Physiol, 2008, 294(2): L152-L160.
14. 刘虹, 李辙, 吕晓希, 等. 中和 IL-17A 活性改善肺纤维化小鼠肺功能. 航天医学与医学工程, 2013, 26(1): 38-42.
15. 中华医学会呼吸病学分会肺功能专业组. 肺功能检查指南(第二部分)——肺量计检查. 中华结核和呼吸杂志, 2014, 37(7): 481-486.
16. 刘加良, 施广懿. 脉冲振荡与通气肺功能二种检测方法比较. 浙江预防医学, 2002, 14(11): 6-8.
17. 鲁惠顺, 卫木根, 胡方. 潮气量与肺顺应性、呼气第一秒容量及压力—容量的相关性. 浙江临床医学, 2001, 3(11): 812-813.
18. 方贤磊, 李杨, 聂李亚, 等. 肺功能分析在放射性肺损伤防治药物药效评价中的作用. 军事医学, 2015, 39(7): 519-522.
19. 杨秀颖, 杜冠华. 组织羟脯氨酸含量微量测定方法及应用. 中国药理学通报, 2004, 20(7): 836-837.
20. 谢伟娜, 丁奇, 孙静, 等. 百部汤对博来霉素诱导小鼠肺纤维化的抑制作用. 中国药科大学学报, 2018, 49(4): 483-489.
21. Chen C, Wang YY, Wang YX, et al. Gentiopicroside ameliorates bleomycin-induced pulmonary fibrosis in mice via inhibiting inflammatory and fibrotic process. Biochem Biophys Res Commun, 2018, 495(4): 2396-2403.
22. 林艺凯, 马爱平, 周伟跃, 等. mTOR 信号通路在博来霉素诱导小鼠肺纤维化中的作用机制研究. 中国呼吸与危重监护杂志, 2018, 17(2): 178-182.
23. 马爱平, 高云周, 兰文斌, 等. PI3K/Akt/HIF-1α 信号通路在博来霉素诱导小鼠肺纤维化中的作用机制研究. 中国呼吸与危重监护杂志, 2016, 15(1): 34-38.

Chinese Journal of Respiratory and Critical Care Medicine

Effect of ampelopsin in mice with bleomycin-induced pulmonary fibrosis

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