Effects of short-time hyperoxia ventilation on lung tissue and pulmonary surfactant proteins C and D in rats_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

WANG Xiaoxia ,  LI Yulan , SHA Xiaolan , WANG Jingjing

Department of Anesthesiology, First Hospital of Lanzhou University, Lanzhou, Gansu 730000, P. R. China;

Corresponding author：

LI Yulan, Email: east_tale@aliyun.com

Keywords：

Hyperoxia; Pulmonary surfactant protein C; Pulmonary surfactant protein D

DOI：

10.7507/1671-6205.201906057

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Objective To investigate the effects of short-time hyperoxia ventilation on lung tissue and pulmonary surfactant proteins C and D (SP-C and SP-D) in rats.Methods Sixteen male Sprague-Dawley rats were randomly divided into two groups (n=8): hyperoxia group (FiO₂=0.90), air group (FiO₂=0.21). Tracheal intubations were administrated after anesthesia, and rats in two groups were exposed hyperoxia or air ventilation for 4 h. At the same time, carotid artery blood gas was analyzed after 2 h and 4 h of ventilation, then oxygenation index (OI) was calculated. Four hours later, the anterior lobe of right lung was taken to observe the pathological change and the injury level was scored. The middle lobe of right lung was prepared for making tissue homogenate, and the remaining part of the lung was used to measure the wet/dry weight (W/D) ratio. The bronchoalveolar lavage fluid (BALF) was prepared in left lung. The content of SP-C and SP-D were detected in lung tissue homogenate and BALF by ELISA.Results Comparing with hyperoxia group, the arterial partial pressure of oxygen, lung histopathology score and lung W/D ratio in air group were significantly increased (P<0.05), but OI, the content of SP-C and SP-D in lung tissue homogenate and BALF were significantly decreased (P<0.05).Conclusion Hyperoxia ventilation for 4 h in rats can cause lung injury histologically, and reduce the concentration of SP-C and SP-D apparently in the lungs.

Citation： WANG Xiaoxia, LI Yulan, SHA Xiaolan, WANG Jingjing. Effects of short-time hyperoxia ventilation on lung tissue and pulmonary surfactant proteins C and D in rats. Chinese Journal of Respiratory and Critical Care Medicine, 2021, 20(1): 32-36. doi: 10.7507/1671-6205.201906057 Copy

1.	Sabaté S, Mazo V, Canet J. Predicting postoperative pulmonary complications: implications for outcomes and costs. Curr Opin Anaesthesiol, 2014, 27(2): 201-209.
2.	Meyhoff CS, Staehr AK, Rasmussen LS. Rational use of oxygen in medical disease and anesthesia. Curr Opin Anaesthesiol, 2012, 25(3): 363-370.
3.	Barkauskas CE, Cronce MJ, Rackley CR, et al. Type 2 alveolar cells are stem cells in adult lung. J Clin Invest, 2013, 123(7): 3025-3036.
4.	Chroneos ZC, Sever-Chroneos Z, Shepherd VL. Pulmonary surfactant: an immunological perspective. Cell Physiol Biochem, 2010, 25(1): 13-26.
5.	Tantawy AA, Adly AA, Ebeid FSE, et al. Surfactant protein D as a marker for pulmonary complications in pediatric patients with sickle cell disease: Relation to lung function tests. Pediatr Pulmonol, 2019, 54(5): 610-619.
6.	Du J, Abdel-Razek O, Shi Q, et al. Surfactant protein D attenuates acute lung and kidney injuries in pneumonia-induced sepsis through modulating apoptosis, inflammation and NF-κB signaling. Sci Rep, 2018, 8: 15393-15406.
7.	Jin Y, Peng LQ, Zhao AL. Hyperoxia induces the apoptosis of alveolar epithelial cells and changes of pulmonary surfactant proteins. Eur Rev Med Pharmacol Sci, 2018, 22(2): 492-497.
8.	Suzuki S, Mihara Y, Hikasa Y, et al. Current ventilator and oxygen management during general anesthesia: a multicenter, cross-sectional observational study. Anesthesiology, 2018, 129(1): 67-76.
9.	Wolthuis EK, Choi G, Dessing MC, et al. Mechanical ventilation with lower tidal volumes and positive end-expiratory pressure prevents pulmonary inflammation in patients without preexisting lung injury. Anesthesiology, 2008, 108(1): 46-54.
10.	Ozdemir R, Demirtas G, Parlakpinar H, et al. Dexpanthenol therapy reduces lung damage in a hyperoxic lung injury in neonatal rats. J Matern Fetal Neonatal Med, 2016, 29(11): 1801-1807.
11.	柳娟, 贾兴元, 张晴, 等. 异丙酚和七氟烷对全麻病人短时间吸入纯氧诱发氧化应激反应的影响. 中华麻醉学杂志, 2010, 30(3): 297-299.
12.	Sinclair SE, Altemeier WA, Matute-Bello G, et al. Augmented lung injury due to interaction between hyperoxia and mechanical ventilation. Crit Care Med, 2004, 32(12): 2496-2501.
13.	Page D, Ablordeppey E, Wessman BT, et al. Emergency department hyperoxia is associated with increased mortality in mechanically ventilated patients: a cohort study. Crit Care, 2018, 22(1): 9.
14.	Helmerhorst HJF, Schouten LRA, Wagenaar GTM, et al. Hyperoxia provokes a time- and dose-dependent inflammatory response in mechanically ventilated mice, irrespective of tidal volumes. Intensive Care Med Exp, 2017, 5(1): 27.
15.	Ferguson ND, Fan E, Camporota L, et al. The Berlin definition of ARDS: an expanded rationale, justification, and supplementary material. Intensive Care Med, 2012, 38(10): 1573-1582.
16.	Villar J, Ambrós A, Soler JA, et al. Age, PaO₂/FIO₂, and plateau pressure score: a proposal for a simple outcome score in patients with the acute respiratory distress syndrome. Crit Care Med, 2016, 44(7): 1361-1369.
17.	Esteve F, Lopez-Delgado JC, Javierre C, et al. Evaluation of the PaO₂/FiO₂ ratio after cardiac surgery as a predictor of outcome during hospital stay. BMC Anesthesiol, 2014, 14: 83.
18.	Aboab J, Louis B, Jonson B, et al. Relation between PaO₂/FiO₂ ratio and FiO₂: a mathematical description. Intensive Care Med, 2006, 32(10): 1494-1497.
19.	Pace PW, Yao LJ, Wilson JX, et al. The effects of hyperoxia exposure on lung function and pulmonary surfactant in a rat model of acute lung injury. Exp Lung Res, 2009, 35(5): 380-398.
20.	Gupta A, Zheng SL. Genetic disorders of surfactant protein dysfunction: when to consider and how to investigate. Arch Dis Child, 2017, 102(1): 84-90.
21.	Zhang L, Zhao S, Yuan LJ, et al. Autophagy regulates hyperoxia-induced intracellular accumulation of surfactant protein C in alveolar type Ⅱ cells. Mol Cell Biochem, 2015, 408(1-2): 181-189.
22.	Jain D, Atochina-Vasserman EN, Tomer Y, et al. Surfactant protein D protects against acute hyperoxic lung injury. Am J Respir Crit Care Med, 2008, 178(8): 805-813.

1. Sabaté S, Mazo V, Canet J. Predicting postoperative pulmonary complications: implications for outcomes and costs. Curr Opin Anaesthesiol, 2014, 27(2): 201-209.
2. Meyhoff CS, Staehr AK, Rasmussen LS. Rational use of oxygen in medical disease and anesthesia. Curr Opin Anaesthesiol, 2012, 25(3): 363-370.
3. Barkauskas CE, Cronce MJ, Rackley CR, et al. Type 2 alveolar cells are stem cells in adult lung. J Clin Invest, 2013, 123(7): 3025-3036.
4. Chroneos ZC, Sever-Chroneos Z, Shepherd VL. Pulmonary surfactant: an immunological perspective. Cell Physiol Biochem, 2010, 25(1): 13-26.
5. Tantawy AA, Adly AA, Ebeid FSE, et al. Surfactant protein D as a marker for pulmonary complications in pediatric patients with sickle cell disease: Relation to lung function tests. Pediatr Pulmonol, 2019, 54(5): 610-619.
6. Du J, Abdel-Razek O, Shi Q, et al. Surfactant protein D attenuates acute lung and kidney injuries in pneumonia-induced sepsis through modulating apoptosis, inflammation and NF-κB signaling. Sci Rep, 2018, 8: 15393-15406.
7. Jin Y, Peng LQ, Zhao AL. Hyperoxia induces the apoptosis of alveolar epithelial cells and changes of pulmonary surfactant proteins. Eur Rev Med Pharmacol Sci, 2018, 22(2): 492-497.
8. Suzuki S, Mihara Y, Hikasa Y, et al. Current ventilator and oxygen management during general anesthesia: a multicenter, cross-sectional observational study. Anesthesiology, 2018, 129(1): 67-76.
9. Wolthuis EK, Choi G, Dessing MC, et al. Mechanical ventilation with lower tidal volumes and positive end-expiratory pressure prevents pulmonary inflammation in patients without preexisting lung injury. Anesthesiology, 2008, 108(1): 46-54.
10. Ozdemir R, Demirtas G, Parlakpinar H, et al. Dexpanthenol therapy reduces lung damage in a hyperoxic lung injury in neonatal rats. J Matern Fetal Neonatal Med, 2016, 29(11): 1801-1807.
11. 柳娟, 贾兴元, 张晴, 等. 异丙酚和七氟烷对全麻病人短时间吸入纯氧诱发氧化应激反应的影响. 中华麻醉学杂志, 2010, 30(3): 297-299.
12. Sinclair SE, Altemeier WA, Matute-Bello G, et al. Augmented lung injury due to interaction between hyperoxia and mechanical ventilation. Crit Care Med, 2004, 32(12): 2496-2501.
13. Page D, Ablordeppey E, Wessman BT, et al. Emergency department hyperoxia is associated with increased mortality in mechanically ventilated patients: a cohort study. Crit Care, 2018, 22(1): 9.
14. Helmerhorst HJF, Schouten LRA, Wagenaar GTM, et al. Hyperoxia provokes a time- and dose-dependent inflammatory response in mechanically ventilated mice, irrespective of tidal volumes. Intensive Care Med Exp, 2017, 5(1): 27.
15. Ferguson ND, Fan E, Camporota L, et al. The Berlin definition of ARDS: an expanded rationale, justification, and supplementary material. Intensive Care Med, 2012, 38(10): 1573-1582.
16. Villar J, Ambrós A, Soler JA, et al. Age, PaO₂/FIO₂, and plateau pressure score: a proposal for a simple outcome score in patients with the acute respiratory distress syndrome. Crit Care Med, 2016, 44(7): 1361-1369.
17. Esteve F, Lopez-Delgado JC, Javierre C, et al. Evaluation of the PaO₂/FiO₂ ratio after cardiac surgery as a predictor of outcome during hospital stay. BMC Anesthesiol, 2014, 14: 83.
18. Aboab J, Louis B, Jonson B, et al. Relation between PaO₂/FiO₂ ratio and FiO₂: a mathematical description. Intensive Care Med, 2006, 32(10): 1494-1497.
19. Pace PW, Yao LJ, Wilson JX, et al. The effects of hyperoxia exposure on lung function and pulmonary surfactant in a rat model of acute lung injury. Exp Lung Res, 2009, 35(5): 380-398.
20. Gupta A, Zheng SL. Genetic disorders of surfactant protein dysfunction: when to consider and how to investigate. Arch Dis Child, 2017, 102(1): 84-90.
21. Zhang L, Zhao S, Yuan LJ, et al. Autophagy regulates hyperoxia-induced intracellular accumulation of surfactant protein C in alveolar type Ⅱ cells. Mol Cell Biochem, 2015, 408(1-2): 181-189.
22. Jain D, Atochina-Vasserman EN, Tomer Y, et al. Surfactant protein D protects against acute hyperoxic lung injury. Am J Respir Crit Care Med, 2008, 178(8): 805-813.

Chinese Journal of Respiratory and Critical Care Medicine

Effects of short-time hyperoxia ventilation on lung tissue and pulmonary surfactant proteins C and D in rats

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